Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS19500 and EX31_RS19505 are separated by 50 nucleotides EX31_RS19505 and EX31_RS19510 are separated by 138 nucleotides EX31_RS19510 and EX31_RS19515 are separated by 354 nucleotides
EX31_RS19500: EX31_RS19500 - PTS ascorbate transporter subunit IIC, at 530,872 to 532,248
_RS19500
EX31_RS19505: EX31_RS19505 - PTS sugar transporter subunit IIB, at 532,299 to 532,580
_RS19505
EX31_RS19510: EX31_RS19510 - PTS sugar transporter subunit IIA, at 532,719 to 533,162
_RS19510
EX31_RS19515: EX31_RS19515 - LacI family DNA-binding transcriptional regulator, at 533,517 to 534,533
_RS19515
Position (kb) 532
533
534 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 531.734 kb on + strand, within EX31_RS19500 at 531.734 kb on + strand, within EX31_RS19500 at 531.734 kb on + strand, within EX31_RS19500 at 531.734 kb on + strand, within EX31_RS19500 at 531.735 kb on - strand, within EX31_RS19500 at 531.735 kb on - strand, within EX31_RS19500 at 531.735 kb on - strand, within EX31_RS19500 at 531.735 kb on - strand, within EX31_RS19500 at 531.735 kb on - strand, within EX31_RS19500 at 531.735 kb on - strand, within EX31_RS19500 at 531.735 kb on - strand, within EX31_RS19500 at 531.846 kb on + strand, within EX31_RS19500 at 531.847 kb on - strand, within EX31_RS19500 at 531.847 kb on - strand, within EX31_RS19500 at 531.847 kb on - strand, within EX31_RS19500 at 531.847 kb on - strand, within EX31_RS19500 at 531.847 kb on - strand, within EX31_RS19500 at 531.847 kb on - strand, within EX31_RS19500 at 531.847 kb on - strand, within EX31_RS19500 at 531.856 kb on - strand, within EX31_RS19500 at 531.857 kb on + strand, within EX31_RS19500 at 531.857 kb on + strand, within EX31_RS19500 at 531.857 kb on + strand, within EX31_RS19500 at 531.858 kb on - strand, within EX31_RS19500 at 531.858 kb on - strand, within EX31_RS19500 at 531.858 kb on - strand, within EX31_RS19500 at 531.858 kb on - strand, within EX31_RS19500 at 531.858 kb on - strand, within EX31_RS19500 at 531.888 kb on - strand, within EX31_RS19500 at 531.888 kb on - strand, within EX31_RS19500 at 531.888 kb on - strand, within EX31_RS19500 at 531.891 kb on + strand, within EX31_RS19500 at 531.892 kb on - strand, within EX31_RS19500 at 531.947 kb on - strand, within EX31_RS19500 at 531.947 kb on - strand, within EX31_RS19500 at 532.009 kb on - strand, within EX31_RS19500 at 532.009 kb on - strand, within EX31_RS19500 at 532.011 kb on + strand, within EX31_RS19500 at 532.058 kb on - strand, within EX31_RS19500 at 532.070 kb on - strand, within EX31_RS19500 at 532.096 kb on + strand, within EX31_RS19500 at 532.097 kb on - strand, within EX31_RS19500 at 532.109 kb on - strand, within EX31_RS19500 at 532.193 kb on - strand at 532.210 kb on + strand at 532.211 kb on - strand at 532.212 kb on + strand at 532.249 kb on - strand at 532.249 kb on - strand at 532.249 kb on - strand at 532.368 kb on - strand, within EX31_RS19505 at 532.370 kb on + strand, within EX31_RS19505 at 532.371 kb on - strand, within EX31_RS19505 at 532.380 kb on + strand, within EX31_RS19505 at 532.381 kb on - strand, within EX31_RS19505 at 532.536 kb on - strand, within EX31_RS19505 at 532.584 kb on - strand at 532.653 kb on - strand at 532.653 kb on - strand at 532.708 kb on - strand at 532.711 kb on - strand at 532.720 kb on + strand at 532.732 kb on - strand at 532.734 kb on - strand at 532.771 kb on - strand, within EX31_RS19510 at 532.829 kb on + strand, within EX31_RS19510 at 532.830 kb on - strand, within EX31_RS19510 at 532.870 kb on + strand, within EX31_RS19510 at 532.870 kb on + strand, within EX31_RS19510 at 532.871 kb on - strand, within EX31_RS19510 at 532.871 kb on - strand, within EX31_RS19510 at 533.000 kb on - strand, within EX31_RS19510 at 533.031 kb on - strand, within EX31_RS19510 at 533.042 kb on - strand, within EX31_RS19510 at 533.052 kb on - strand, within EX31_RS19510 at 533.162 kb on + strand at 533.162 kb on + strand at 533.162 kb on + strand at 533.163 kb on - strand at 533.163 kb on - strand at 533.163 kb on - strand at 533.163 kb on - strand at 533.163 kb on - strand at 533.185 kb on + strand at 533.235 kb on + strand at 533.235 kb on + strand at 533.235 kb on + strand at 533.235 kb on + strand at 533.235 kb on + strand at 533.236 kb on - strand at 533.246 kb on + strand at 533.253 kb on - strand at 533.265 kb on - strand at 533.279 kb on + strand at 533.320 kb on + strand at 533.365 kb on + strand at 533.366 kb on - strand at 533.369 kb on + strand at 533.370 kb on - strand at 533.375 kb on + strand at 533.377 kb on + strand at 533.377 kb on + strand at 533.377 kb on + strand at 533.377 kb on + strand at 533.377 kb on + strand at 533.377 kb on + strand at 533.377 kb on + strand at 533.377 kb on + strand at 533.377 kb on + strand at 533.378 kb on - strand at 533.378 kb on - strand at 533.378 kb on - strand at 533.378 kb on - strand at 533.404 kb on + strand at 533.464 kb on + strand at 533.464 kb on + strand at 533.476 kb on + strand at 533.476 kb on + strand at 533.477 kb on - strand at 533.477 kb on - strand at 533.477 kb on - strand at 533.477 kb on - strand at 533.500 kb on + strand at 533.500 kb on + strand at 533.500 kb on + strand at 533.500 kb on + strand at 533.500 kb on + strand at 533.500 kb on + strand at 533.501 kb on - strand at 533.501 kb on - strand at 533.525 kb on + strand at 533.645 kb on - strand, within EX31_RS19515 at 533.645 kb on - strand, within EX31_RS19515 at 533.729 kb on + strand, within EX31_RS19515 at 533.729 kb on + strand, within EX31_RS19515 at 533.730 kb on - strand, within EX31_RS19515 at 533.730 kb on - strand, within EX31_RS19515 at 533.849 kb on + strand, within EX31_RS19515 at 533.849 kb on + strand, within EX31_RS19515 at 533.889 kb on + strand, within EX31_RS19515 at 533.889 kb on + strand, within EX31_RS19515 at 533.890 kb on - strand, within EX31_RS19515 at 533.938 kb on + strand, within EX31_RS19515 at 533.938 kb on + strand, within EX31_RS19515 at 533.938 kb on + strand, within EX31_RS19515 at 533.939 kb on - strand, within EX31_RS19515 at 533.939 kb on - strand, within EX31_RS19515 at 534.027 kb on + strand, within EX31_RS19515 at 534.027 kb on + strand, within EX31_RS19515 at 534.028 kb on - strand, within EX31_RS19515 at 534.147 kb on - strand, within EX31_RS19515 at 534.147 kb on - strand, within EX31_RS19515
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 531,734 + EX31_RS19500 0.63 -0.3 531,734 + EX31_RS19500 0.63 +1.0 531,734 + EX31_RS19500 0.63 -1.5 531,734 + EX31_RS19500 0.63 +1.9 531,735 - EX31_RS19500 0.63 -0.2 531,735 - EX31_RS19500 0.63 +0.2 531,735 - EX31_RS19500 0.63 +0.8 531,735 - EX31_RS19500 0.63 -1.9 531,735 - EX31_RS19500 0.63 +0.2 531,735 - EX31_RS19500 0.63 -0.2 531,735 - EX31_RS19500 0.63 +0.3 531,846 + EX31_RS19500 0.71 +0.3 531,847 - EX31_RS19500 0.71 +0.7 531,847 - EX31_RS19500 0.71 +1.1 531,847 - EX31_RS19500 0.71 +0.5 531,847 - EX31_RS19500 0.71 +0.2 531,847 - EX31_RS19500 0.71 +1.0 531,847 - EX31_RS19500 0.71 +1.3 531,847 - EX31_RS19500 0.71 +0.7 531,856 - EX31_RS19500 0.71 -0.8 531,857 + EX31_RS19500 0.72 +1.1 531,857 + EX31_RS19500 0.72 -0.7 531,857 + EX31_RS19500 0.72 +1.0 531,858 - EX31_RS19500 0.72 -0.3 531,858 - EX31_RS19500 0.72 +0.8 531,858 - EX31_RS19500 0.72 -0.4 531,858 - EX31_RS19500 0.72 +0.1 531,858 - EX31_RS19500 0.72 +0.0 531,888 - EX31_RS19500 0.74 +0.1 531,888 - EX31_RS19500 0.74 -0.5 531,888 - EX31_RS19500 0.74 +2.4 531,891 + EX31_RS19500 0.74 +1.6 531,892 - EX31_RS19500 0.74 -0.1 531,947 - EX31_RS19500 0.78 +1.0 531,947 - EX31_RS19500 0.78 +0.3 532,009 - EX31_RS19500 0.83 +1.8 532,009 - EX31_RS19500 0.83 -0.0 532,011 + EX31_RS19500 0.83 +0.9 532,058 - EX31_RS19500 0.86 +0.3 532,070 - EX31_RS19500 0.87 +0.1 532,096 + EX31_RS19500 0.89 +2.5 532,097 - EX31_RS19500 0.89 +0.7 532,109 - EX31_RS19500 0.90 +0.4 532,193 - +0.5 532,210 + +1.0 532,211 - +0.4 532,212 + -0.2 532,249 - -2.9 532,249 - -1.4 532,249 - +1.5 532,368 - EX31_RS19505 0.24 +0.4 532,370 + EX31_RS19505 0.25 +0.8 532,371 - EX31_RS19505 0.26 -0.5 532,380 + EX31_RS19505 0.29 +3.5 532,381 - EX31_RS19505 0.29 +0.2 532,536 - EX31_RS19505 0.84 +0.2 532,584 - +1.1 532,653 - -0.5 532,653 - +0.1 532,708 - -0.3 532,711 - +0.6 532,720 + +1.5 532,732 - -0.5 532,734 - +0.5 532,771 - EX31_RS19510 0.12 -0.7 532,829 + EX31_RS19510 0.25 +0.1 532,830 - EX31_RS19510 0.25 -0.2 532,870 + EX31_RS19510 0.34 -0.6 532,870 + EX31_RS19510 0.34 -0.1 532,871 - EX31_RS19510 0.34 +0.5 532,871 - EX31_RS19510 0.34 -0.5 533,000 - EX31_RS19510 0.63 +0.8 533,031 - EX31_RS19510 0.70 -0.2 533,042 - EX31_RS19510 0.73 -0.8 533,052 - EX31_RS19510 0.75 +0.6 533,162 + +1.5 533,162 + -1.2 533,162 + -0.2 533,163 - +0.5 533,163 - -0.9 533,163 - -2.0 533,163 - +0.6 533,163 - +1.1 533,185 + +1.6 533,235 + +0.4 533,235 + +1.1 533,235 + +0.0 533,235 + +1.0 533,235 + -1.3 533,236 - -0.3 533,246 + -0.6 533,253 - -0.1 533,265 - +0.9 533,279 + -1.1 533,320 + -0.1 533,365 + -0.1 533,366 - +0.5 533,369 + +0.5 533,370 - +2.9 533,375 + -0.7 533,377 + +0.9 533,377 + +0.1 533,377 + -0.8 533,377 + +0.2 533,377 + -1.2 533,377 + +0.1 533,377 + -3.0 533,377 + -2.2 533,377 + +1.4 533,378 - -1.2 533,378 - +0.7 533,378 - +1.4 533,378 - +0.0 533,404 + +0.4 533,464 + +0.1 533,464 + -0.3 533,476 + +0.1 533,476 + +1.0 533,477 - +1.4 533,477 - +0.6 533,477 - +1.9 533,477 - +0.9 533,500 + -2.1 533,500 + -1.2 533,500 + +0.3 533,500 + +0.4 533,500 + -0.9 533,500 + -0.1 533,501 - +0.9 533,501 - -0.2 533,525 + +1.2 533,645 - EX31_RS19515 0.13 +1.1 533,645 - EX31_RS19515 0.13 +1.0 533,729 + EX31_RS19515 0.21 -2.0 533,729 + EX31_RS19515 0.21 -2.5 533,730 - EX31_RS19515 0.21 -0.4 533,730 - EX31_RS19515 0.21 +0.9 533,849 + EX31_RS19515 0.33 -0.8 533,849 + EX31_RS19515 0.33 +0.2 533,889 + EX31_RS19515 0.37 +0.6 533,889 + EX31_RS19515 0.37 -0.9 533,890 - EX31_RS19515 0.37 +0.7 533,938 + EX31_RS19515 0.41 +0.9 533,938 + EX31_RS19515 0.41 -0.9 533,938 + EX31_RS19515 0.41 -0.3 533,939 - EX31_RS19515 0.41 -0.6 533,939 - EX31_RS19515 0.41 -0.6 534,027 + EX31_RS19515 0.50 +0.6 534,027 + EX31_RS19515 0.50 +0.6 534,028 - EX31_RS19515 0.50 +0.6 534,147 - EX31_RS19515 0.62 +0.1 534,147 - EX31_RS19515 0.62 +0.4
Or see this region's nucleotide sequence