Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS17720 and EX31_RS17725 are separated by 123 nucleotides EX31_RS17725 and EX31_RS17730 are separated by 18 nucleotides
EX31_RS17720: EX31_RS17720 - porin, at 96,700 to 98,052
_RS17720
EX31_RS17725: EX31_RS17725 - glycoside hydrolase family 32 protein, at 98,176 to 99,609
_RS17725
EX31_RS17730: EX31_RS17730 - MFS transporter, at 99,628 to 100,869
_RS17730
Position (kb) 98
99
100 Strain fitness (log2 ratio) -2
-1
0
1
2 at 97.244 kb on + strand, within EX31_RS17720 at 97.245 kb on - strand, within EX31_RS17720 at 97.245 kb on - strand, within EX31_RS17720 at 97.245 kb on - strand, within EX31_RS17720 at 97.264 kb on - strand, within EX31_RS17720 at 97.267 kb on + strand, within EX31_RS17720 at 97.283 kb on + strand, within EX31_RS17720 at 97.284 kb on - strand, within EX31_RS17720 at 97.287 kb on + strand, within EX31_RS17720 at 97.287 kb on + strand, within EX31_RS17720 at 97.287 kb on + strand, within EX31_RS17720 at 97.287 kb on + strand, within EX31_RS17720 at 97.288 kb on - strand, within EX31_RS17720 at 97.288 kb on - strand, within EX31_RS17720 at 97.288 kb on - strand, within EX31_RS17720 at 97.288 kb on - strand, within EX31_RS17720 at 97.318 kb on + strand, within EX31_RS17720 at 97.318 kb on + strand, within EX31_RS17720 at 97.320 kb on + strand, within EX31_RS17720 at 97.321 kb on - strand, within EX31_RS17720 at 97.321 kb on - strand, within EX31_RS17720 at 97.359 kb on + strand, within EX31_RS17720 at 97.415 kb on + strand, within EX31_RS17720 at 97.415 kb on + strand, within EX31_RS17720 at 97.416 kb on - strand, within EX31_RS17720 at 97.416 kb on - strand, within EX31_RS17720 at 97.416 kb on - strand, within EX31_RS17720 at 97.416 kb on - strand, within EX31_RS17720 at 97.416 kb on - strand, within EX31_RS17720 at 97.416 kb on - strand, within EX31_RS17720 at 97.416 kb on - strand, within EX31_RS17720 at 97.419 kb on + strand, within EX31_RS17720 at 97.420 kb on - strand, within EX31_RS17720 at 97.420 kb on - strand, within EX31_RS17720 at 97.420 kb on - strand, within EX31_RS17720 at 97.421 kb on + strand, within EX31_RS17720 at 97.421 kb on + strand, within EX31_RS17720 at 97.422 kb on - strand, within EX31_RS17720 at 97.422 kb on - strand, within EX31_RS17720 at 97.440 kb on - strand, within EX31_RS17720 at 97.479 kb on + strand, within EX31_RS17720 at 97.480 kb on - strand, within EX31_RS17720 at 97.480 kb on - strand, within EX31_RS17720 at 97.501 kb on - strand, within EX31_RS17720 at 97.523 kb on - strand, within EX31_RS17720 at 97.592 kb on - strand, within EX31_RS17720 at 97.593 kb on + strand, within EX31_RS17720 at 97.625 kb on + strand, within EX31_RS17720 at 97.672 kb on - strand, within EX31_RS17720 at 97.672 kb on - strand, within EX31_RS17720 at 97.684 kb on - strand, within EX31_RS17720 at 97.698 kb on + strand, within EX31_RS17720 at 97.777 kb on - strand, within EX31_RS17720 at 97.807 kb on - strand, within EX31_RS17720 at 97.807 kb on - strand, within EX31_RS17720 at 97.807 kb on - strand, within EX31_RS17720 at 97.854 kb on + strand, within EX31_RS17720 at 97.854 kb on + strand, within EX31_RS17720 at 97.861 kb on + strand, within EX31_RS17720 at 97.865 kb on - strand, within EX31_RS17720 at 97.986 kb on + strand at 97.987 kb on - strand at 98.011 kb on - strand at 98.014 kb on + strand at 98.014 kb on + strand at 98.015 kb on - strand at 98.015 kb on - strand at 98.015 kb on - strand at 98.015 kb on - strand at 98.028 kb on + strand at 98.028 kb on + strand at 98.028 kb on + strand at 98.029 kb on - strand at 98.029 kb on - strand at 98.029 kb on - strand at 98.029 kb on - strand at 98.069 kb on - strand at 98.071 kb on + strand at 98.072 kb on - strand at 98.076 kb on - strand at 98.078 kb on - strand at 98.080 kb on + strand at 98.080 kb on + strand at 98.081 kb on - strand at 98.081 kb on - strand at 98.103 kb on + strand at 98.104 kb on - strand at 98.264 kb on + strand at 98.267 kb on - strand at 98.306 kb on - strand at 98.306 kb on - strand at 98.339 kb on - strand, within EX31_RS17725 at 98.386 kb on + strand, within EX31_RS17725 at 98.386 kb on + strand, within EX31_RS17725 at 98.387 kb on - strand, within EX31_RS17725 at 98.387 kb on - strand, within EX31_RS17725 at 98.393 kb on + strand, within EX31_RS17725 at 98.444 kb on + strand, within EX31_RS17725 at 98.444 kb on + strand, within EX31_RS17725 at 98.445 kb on - strand, within EX31_RS17725 at 98.445 kb on - strand, within EX31_RS17725 at 98.445 kb on - strand, within EX31_RS17725 at 98.469 kb on + strand, within EX31_RS17725 at 98.575 kb on - strand, within EX31_RS17725 at 98.727 kb on + strand, within EX31_RS17725 at 98.728 kb on - strand, within EX31_RS17725 at 98.728 kb on - strand, within EX31_RS17725 at 98.787 kb on - strand, within EX31_RS17725 at 98.787 kb on - strand, within EX31_RS17725 at 98.886 kb on + strand, within EX31_RS17725 at 98.886 kb on + strand, within EX31_RS17725 at 98.887 kb on - strand, within EX31_RS17725 at 98.950 kb on - strand, within EX31_RS17725 at 98.979 kb on + strand, within EX31_RS17725 at 98.980 kb on - strand, within EX31_RS17725 at 99.050 kb on + strand, within EX31_RS17725 at 99.050 kb on + strand, within EX31_RS17725 at 99.051 kb on - strand, within EX31_RS17725 at 99.051 kb on - strand, within EX31_RS17725 at 99.058 kb on + strand, within EX31_RS17725 at 99.058 kb on + strand, within EX31_RS17725 at 99.059 kb on - strand, within EX31_RS17725 at 99.059 kb on - strand, within EX31_RS17725 at 99.059 kb on - strand, within EX31_RS17725 at 99.059 kb on - strand, within EX31_RS17725 at 99.098 kb on + strand, within EX31_RS17725 at 99.098 kb on + strand, within EX31_RS17725 at 99.099 kb on - strand, within EX31_RS17725 at 99.099 kb on - strand, within EX31_RS17725 at 99.099 kb on - strand, within EX31_RS17725 at 99.099 kb on - strand, within EX31_RS17725 at 99.106 kb on - strand, within EX31_RS17725 at 99.106 kb on - strand, within EX31_RS17725 at 99.192 kb on + strand, within EX31_RS17725 at 99.192 kb on + strand, within EX31_RS17725 at 99.264 kb on + strand, within EX31_RS17725 at 99.275 kb on + strand, within EX31_RS17725 at 99.438 kb on - strand, within EX31_RS17725 at 99.438 kb on - strand, within EX31_RS17725 at 99.452 kb on + strand, within EX31_RS17725 at 99.465 kb on - strand, within EX31_RS17725 at 99.501 kb on + strand at 99.600 kb on + strand at 99.601 kb on - strand at 99.601 kb on - strand at 99.601 kb on - strand at 99.601 kb on - strand at 99.601 kb on - strand at 99.601 kb on - strand at 99.602 kb on + strand at 99.633 kb on + strand at 99.636 kb on + strand at 99.637 kb on - strand at 99.650 kb on - strand at 99.693 kb on + strand at 99.693 kb on + strand at 99.700 kb on - strand at 99.709 kb on - strand at 99.738 kb on - strand at 99.756 kb on - strand, within EX31_RS17730 at 99.764 kb on + strand, within EX31_RS17730 at 99.764 kb on + strand, within EX31_RS17730 at 99.814 kb on - strand, within EX31_RS17730 at 99.833 kb on + strand, within EX31_RS17730 at 99.834 kb on - strand, within EX31_RS17730 at 99.864 kb on + strand, within EX31_RS17730 at 99.865 kb on - strand, within EX31_RS17730 at 99.875 kb on + strand, within EX31_RS17730 at 99.876 kb on - strand, within EX31_RS17730 at 99.885 kb on + strand, within EX31_RS17730 at 99.892 kb on + strand, within EX31_RS17730 at 99.928 kb on - strand, within EX31_RS17730 at 99.930 kb on + strand, within EX31_RS17730 at 99.931 kb on - strand, within EX31_RS17730 at 99.939 kb on + strand, within EX31_RS17730 at 99.940 kb on - strand, within EX31_RS17730 at 99.940 kb on - strand, within EX31_RS17730 at 100.089 kb on + strand, within EX31_RS17730 at 100.090 kb on - strand, within EX31_RS17730 at 100.100 kb on + strand, within EX31_RS17730 at 100.100 kb on + strand, within EX31_RS17730 at 100.100 kb on + strand, within EX31_RS17730 at 100.100 kb on + strand, within EX31_RS17730 at 100.100 kb on + strand, within EX31_RS17730 at 100.100 kb on + strand, within EX31_RS17730 at 100.100 kb on + strand, within EX31_RS17730 at 100.100 kb on + strand, within EX31_RS17730 at 100.101 kb on - strand, within EX31_RS17730 at 100.101 kb on - strand, within EX31_RS17730 at 100.101 kb on - strand, within EX31_RS17730 at 100.101 kb on - strand, within EX31_RS17730 at 100.101 kb on - strand, within EX31_RS17730 at 100.101 kb on - strand, within EX31_RS17730 at 100.101 kb on - strand, within EX31_RS17730 at 100.166 kb on + strand, within EX31_RS17730 at 100.166 kb on + strand, within EX31_RS17730 at 100.175 kb on + strand, within EX31_RS17730 at 100.176 kb on - strand, within EX31_RS17730 at 100.176 kb on - strand, within EX31_RS17730 at 100.176 kb on - strand, within EX31_RS17730 at 100.179 kb on + strand, within EX31_RS17730 at 100.179 kb on + strand, within EX31_RS17730 at 100.179 kb on + strand, within EX31_RS17730 at 100.384 kb on - strand, within EX31_RS17730 at 100.488 kb on - strand, within EX31_RS17730 at 100.488 kb on - strand, within EX31_RS17730 at 100.498 kb on + strand, within EX31_RS17730 at 100.499 kb on - strand, within EX31_RS17730 at 100.499 kb on - strand, within EX31_RS17730 at 100.532 kb on + strand, within EX31_RS17730 at 100.533 kb on - strand, within EX31_RS17730 at 100.533 kb on - strand, within EX31_RS17730 at 100.566 kb on + strand, within EX31_RS17730 at 100.567 kb on - strand, within EX31_RS17730 at 100.595 kb on + strand, within EX31_RS17730 at 100.596 kb on - strand, within EX31_RS17730
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 97,244 + EX31_RS17720 0.40 +0.3 97,245 - EX31_RS17720 0.40 +0.5 97,245 - EX31_RS17720 0.40 +1.0 97,245 - EX31_RS17720 0.40 -1.5 97,264 - EX31_RS17720 0.42 +0.9 97,267 + EX31_RS17720 0.42 +0.1 97,283 + EX31_RS17720 0.43 -1.6 97,284 - EX31_RS17720 0.43 +0.4 97,287 + EX31_RS17720 0.43 +0.1 97,287 + EX31_RS17720 0.43 +1.2 97,287 + EX31_RS17720 0.43 +1.1 97,287 + EX31_RS17720 0.43 +0.7 97,288 - EX31_RS17720 0.43 +0.7 97,288 - EX31_RS17720 0.43 +0.7 97,288 - EX31_RS17720 0.43 -0.4 97,288 - EX31_RS17720 0.43 +0.4 97,318 + EX31_RS17720 0.46 -1.3 97,318 + EX31_RS17720 0.46 +0.8 97,320 + EX31_RS17720 0.46 +1.5 97,321 - EX31_RS17720 0.46 +0.4 97,321 - EX31_RS17720 0.46 -0.0 97,359 + EX31_RS17720 0.49 +0.2 97,415 + EX31_RS17720 0.53 +0.5 97,415 + EX31_RS17720 0.53 +2.0 97,416 - EX31_RS17720 0.53 -0.5 97,416 - EX31_RS17720 0.53 +1.0 97,416 - EX31_RS17720 0.53 +0.7 97,416 - EX31_RS17720 0.53 -1.2 97,416 - EX31_RS17720 0.53 +0.6 97,416 - EX31_RS17720 0.53 +1.3 97,416 - EX31_RS17720 0.53 -2.0 97,419 + EX31_RS17720 0.53 -0.9 97,420 - EX31_RS17720 0.53 +1.2 97,420 - EX31_RS17720 0.53 +0.1 97,420 - EX31_RS17720 0.53 +1.5 97,421 + EX31_RS17720 0.53 -0.2 97,421 + EX31_RS17720 0.53 +0.1 97,422 - EX31_RS17720 0.53 -0.3 97,422 - EX31_RS17720 0.53 +0.5 97,440 - EX31_RS17720 0.55 +0.0 97,479 + EX31_RS17720 0.58 -0.3 97,480 - EX31_RS17720 0.58 -0.2 97,480 - EX31_RS17720 0.58 +1.7 97,501 - EX31_RS17720 0.59 +1.5 97,523 - EX31_RS17720 0.61 +0.2 97,592 - EX31_RS17720 0.66 -0.1 97,593 + EX31_RS17720 0.66 -0.5 97,625 + EX31_RS17720 0.68 +0.9 97,672 - EX31_RS17720 0.72 -0.6 97,672 - EX31_RS17720 0.72 -0.3 97,684 - EX31_RS17720 0.73 -0.3 97,698 + EX31_RS17720 0.74 -0.1 97,777 - EX31_RS17720 0.80 -1.2 97,807 - EX31_RS17720 0.82 -1.5 97,807 - EX31_RS17720 0.82 +1.2 97,807 - EX31_RS17720 0.82 +1.1 97,854 + EX31_RS17720 0.85 +2.4 97,854 + EX31_RS17720 0.85 +0.9 97,861 + EX31_RS17720 0.86 -1.1 97,865 - EX31_RS17720 0.86 -0.6 97,986 + -0.2 97,987 - -0.5 98,011 - -0.6 98,014 + -0.4 98,014 + -1.5 98,015 - +1.2 98,015 - +0.5 98,015 - +0.8 98,015 - -0.1 98,028 + -0.3 98,028 + +0.5 98,028 + -0.7 98,029 - -0.7 98,029 - -1.1 98,029 - +0.9 98,029 - +0.1 98,069 - -2.0 98,071 + +0.9 98,072 - +1.0 98,076 - -0.4 98,078 - +0.5 98,080 + +0.9 98,080 + +0.1 98,081 - +0.8 98,081 - -0.9 98,103 + -1.6 98,104 - -0.2 98,264 + +0.4 98,267 - -0.3 98,306 - -0.2 98,306 - +0.4 98,339 - EX31_RS17725 0.11 -0.2 98,386 + EX31_RS17725 0.15 +0.5 98,386 + EX31_RS17725 0.15 +0.3 98,387 - EX31_RS17725 0.15 +0.0 98,387 - EX31_RS17725 0.15 +1.5 98,393 + EX31_RS17725 0.15 +2.7 98,444 + EX31_RS17725 0.19 +0.3 98,444 + EX31_RS17725 0.19 -0.1 98,445 - EX31_RS17725 0.19 +0.1 98,445 - EX31_RS17725 0.19 +0.2 98,445 - EX31_RS17725 0.19 +1.0 98,469 + EX31_RS17725 0.20 -0.3 98,575 - EX31_RS17725 0.28 -0.4 98,727 + EX31_RS17725 0.38 -0.6 98,728 - EX31_RS17725 0.38 +0.4 98,728 - EX31_RS17725 0.38 +1.9 98,787 - EX31_RS17725 0.43 +1.3 98,787 - EX31_RS17725 0.43 -0.5 98,886 + EX31_RS17725 0.50 -0.0 98,886 + EX31_RS17725 0.50 +2.1 98,887 - EX31_RS17725 0.50 -1.3 98,950 - EX31_RS17725 0.54 +0.9 98,979 + EX31_RS17725 0.56 +1.3 98,980 - EX31_RS17725 0.56 +1.2 99,050 + EX31_RS17725 0.61 +1.5 99,050 + EX31_RS17725 0.61 +0.3 99,051 - EX31_RS17725 0.61 +1.5 99,051 - EX31_RS17725 0.61 -0.1 99,058 + EX31_RS17725 0.62 +0.0 99,058 + EX31_RS17725 0.62 +1.7 99,059 - EX31_RS17725 0.62 -0.2 99,059 - EX31_RS17725 0.62 +0.4 99,059 - EX31_RS17725 0.62 +0.7 99,059 - EX31_RS17725 0.62 +0.5 99,098 + EX31_RS17725 0.64 +0.1 99,098 + EX31_RS17725 0.64 -0.1 99,099 - EX31_RS17725 0.64 +1.0 99,099 - EX31_RS17725 0.64 -0.5 99,099 - EX31_RS17725 0.64 +0.0 99,099 - EX31_RS17725 0.64 -0.3 99,106 - EX31_RS17725 0.65 +0.9 99,106 - EX31_RS17725 0.65 -1.6 99,192 + EX31_RS17725 0.71 -2.1 99,192 + EX31_RS17725 0.71 +1.1 99,264 + EX31_RS17725 0.76 +0.1 99,275 + EX31_RS17725 0.77 +0.7 99,438 - EX31_RS17725 0.88 +0.9 99,438 - EX31_RS17725 0.88 +0.5 99,452 + EX31_RS17725 0.89 +0.9 99,465 - EX31_RS17725 0.90 +0.4 99,501 + -1.0 99,600 + +0.4 99,601 - +0.9 99,601 - -0.2 99,601 - +0.5 99,601 - -1.3 99,601 - -0.4 99,601 - +0.5 99,602 + +1.0 99,633 + +0.4 99,636 + +1.3 99,637 - -0.4 99,650 - -2.2 99,693 + -1.5 99,693 + +0.4 99,700 - +1.1 99,709 - +0.9 99,738 - +0.6 99,756 - EX31_RS17730 0.10 +0.7 99,764 + EX31_RS17730 0.11 -1.0 99,764 + EX31_RS17730 0.11 +1.1 99,814 - EX31_RS17730 0.15 -1.6 99,833 + EX31_RS17730 0.17 -0.1 99,834 - EX31_RS17730 0.17 +0.6 99,864 + EX31_RS17730 0.19 -1.8 99,865 - EX31_RS17730 0.19 -0.6 99,875 + EX31_RS17730 0.20 +0.2 99,876 - EX31_RS17730 0.20 +0.4 99,885 + EX31_RS17730 0.21 -0.1 99,892 + EX31_RS17730 0.21 -0.3 99,928 - EX31_RS17730 0.24 -1.5 99,930 + EX31_RS17730 0.24 +0.4 99,931 - EX31_RS17730 0.24 +0.4 99,939 + EX31_RS17730 0.25 +0.2 99,940 - EX31_RS17730 0.25 -1.6 99,940 - EX31_RS17730 0.25 +2.5 100,089 + EX31_RS17730 0.37 -1.1 100,090 - EX31_RS17730 0.37 -1.5 100,100 + EX31_RS17730 0.38 -0.6 100,100 + EX31_RS17730 0.38 +0.3 100,100 + EX31_RS17730 0.38 +0.9 100,100 + EX31_RS17730 0.38 +0.3 100,100 + EX31_RS17730 0.38 -1.1 100,100 + EX31_RS17730 0.38 -1.3 100,100 + EX31_RS17730 0.38 +0.7 100,100 + EX31_RS17730 0.38 +0.1 100,101 - EX31_RS17730 0.38 +1.7 100,101 - EX31_RS17730 0.38 +0.7 100,101 - EX31_RS17730 0.38 +0.9 100,101 - EX31_RS17730 0.38 +0.4 100,101 - EX31_RS17730 0.38 +1.1 100,101 - EX31_RS17730 0.38 -1.8 100,101 - EX31_RS17730 0.38 +1.4 100,166 + EX31_RS17730 0.43 +0.7 100,166 + EX31_RS17730 0.43 +0.6 100,175 + EX31_RS17730 0.44 +0.8 100,176 - EX31_RS17730 0.44 -0.9 100,176 - EX31_RS17730 0.44 +0.0 100,176 - EX31_RS17730 0.44 -0.2 100,179 + EX31_RS17730 0.44 +1.1 100,179 + EX31_RS17730 0.44 -0.1 100,179 + EX31_RS17730 0.44 -1.6 100,384 - EX31_RS17730 0.61 -1.0 100,488 - EX31_RS17730 0.69 +1.1 100,488 - EX31_RS17730 0.69 +2.3 100,498 + EX31_RS17730 0.70 -1.3 100,499 - EX31_RS17730 0.70 +0.1 100,499 - EX31_RS17730 0.70 +2.3 100,532 + EX31_RS17730 0.73 -0.8 100,533 - EX31_RS17730 0.73 -0.1 100,533 - EX31_RS17730 0.73 +0.1 100,566 + EX31_RS17730 0.76 +0.5 100,567 - EX31_RS17730 0.76 +0.5 100,595 + EX31_RS17730 0.78 +0.4 100,596 - EX31_RS17730 0.78 -0.9
Or see this region's nucleotide sequence