Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS16835 and EX31_RS16840 are separated by 70 nucleotides EX31_RS16840 and EX31_RS16845 are separated by 190 nucleotides EX31_RS16845 and EX31_RS16850 are separated by 9 nucleotides EX31_RS16850 and EX31_RS16855 are separated by 219 nucleotides
EX31_RS16835: EX31_RS16835 - deoxyribonuclease V, at 21,909 to 22,589
_RS16835
EX31_RS16840: EX31_RS16840 - YjaG family protein, at 22,660 to 23,250
_RS16840
EX31_RS16845: EX31_RS16845 - DNA-binding protein HU-alpha, at 23,441 to 23,713
_RS16845
EX31_RS16850: EX31_RS16850 - DUF1481 domain-containing protein, at 23,723 to 24,418
_RS16850
EX31_RS16855: EX31_RS16855 - phosphoribosylamine--glycine ligase, at 24,638 to 25,927
_RS16855
Position (kb) 23
24 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 22.448 kb on + strand, within EX31_RS16835 at 22.448 kb on + strand, within EX31_RS16835 at 22.448 kb on + strand, within EX31_RS16835 at 22.449 kb on - strand, within EX31_RS16835 at 22.449 kb on - strand, within EX31_RS16835 at 22.470 kb on + strand, within EX31_RS16835 at 22.489 kb on + strand, within EX31_RS16835 at 22.489 kb on + strand, within EX31_RS16835 at 22.490 kb on - strand, within EX31_RS16835 at 22.514 kb on + strand, within EX31_RS16835 at 22.607 kb on + strand at 22.607 kb on + strand at 22.616 kb on - strand at 22.664 kb on + strand at 22.664 kb on + strand at 22.664 kb on + strand at 22.664 kb on + strand at 22.665 kb on - strand at 22.665 kb on - strand at 22.665 kb on - strand at 22.665 kb on - strand at 22.668 kb on + strand at 22.668 kb on + strand at 22.668 kb on + strand at 22.669 kb on - strand at 22.682 kb on + strand at 22.682 kb on + strand at 22.682 kb on + strand at 22.682 kb on + strand at 22.682 kb on + strand at 22.748 kb on - strand, within EX31_RS16840 at 22.756 kb on + strand, within EX31_RS16840 at 22.757 kb on - strand, within EX31_RS16840 at 22.820 kb on + strand, within EX31_RS16840 at 22.841 kb on + strand, within EX31_RS16840 at 22.841 kb on + strand, within EX31_RS16840 at 22.912 kb on + strand, within EX31_RS16840 at 22.912 kb on + strand, within EX31_RS16840 at 22.913 kb on - strand, within EX31_RS16840 at 22.921 kb on + strand, within EX31_RS16840 at 22.921 kb on + strand, within EX31_RS16840 at 22.921 kb on + strand, within EX31_RS16840 at 22.921 kb on + strand, within EX31_RS16840 at 22.921 kb on + strand, within EX31_RS16840 at 22.921 kb on + strand, within EX31_RS16840 at 22.921 kb on + strand, within EX31_RS16840 at 22.922 kb on - strand, within EX31_RS16840 at 22.922 kb on - strand, within EX31_RS16840 at 22.950 kb on + strand, within EX31_RS16840 at 22.950 kb on + strand, within EX31_RS16840 at 22.950 kb on + strand, within EX31_RS16840 at 22.950 kb on + strand, within EX31_RS16840 at 22.950 kb on + strand, within EX31_RS16840 at 22.951 kb on - strand, within EX31_RS16840 at 22.958 kb on + strand, within EX31_RS16840 at 23.180 kb on + strand, within EX31_RS16840 at 23.180 kb on + strand, within EX31_RS16840 at 23.180 kb on + strand, within EX31_RS16840 at 23.180 kb on + strand, within EX31_RS16840 at 23.183 kb on + strand, within EX31_RS16840 at 23.183 kb on + strand, within EX31_RS16840 at 23.248 kb on + strand at 23.248 kb on + strand at 23.249 kb on - strand at 23.268 kb on + strand at 23.268 kb on + strand at 23.269 kb on - strand at 23.360 kb on + strand at 23.360 kb on + strand at 23.360 kb on + strand at 23.361 kb on - strand at 23.387 kb on + strand at 23.428 kb on + strand at 23.428 kb on + strand at 23.429 kb on - strand at 23.429 kb on - strand at 23.435 kb on + strand at 23.436 kb on - strand at 23.440 kb on + strand at 23.440 kb on + strand at 23.440 kb on + strand at 23.441 kb on - strand at 23.441 kb on - strand at 23.441 kb on - strand at 23.441 kb on - strand at 23.441 kb on - strand at 23.441 kb on - strand at 23.466 kb on + strand at 23.466 kb on + strand at 23.521 kb on + strand, within EX31_RS16845 at 23.521 kb on + strand, within EX31_RS16845 at 23.565 kb on + strand, within EX31_RS16845 at 23.565 kb on + strand, within EX31_RS16845 at 23.571 kb on + strand, within EX31_RS16845 at 23.571 kb on + strand, within EX31_RS16845 at 23.595 kb on + strand, within EX31_RS16845 at 23.595 kb on + strand, within EX31_RS16845 at 23.707 kb on + strand at 23.708 kb on - strand at 23.711 kb on + strand at 23.711 kb on + strand at 23.711 kb on + strand at 23.782 kb on - strand at 23.782 kb on - strand at 23.790 kb on + strand at 23.790 kb on + strand at 23.791 kb on - strand at 23.838 kb on + strand, within EX31_RS16850 at 23.838 kb on + strand, within EX31_RS16850 at 23.855 kb on + strand, within EX31_RS16850 at 23.855 kb on + strand, within EX31_RS16850 at 23.856 kb on - strand, within EX31_RS16850 at 23.873 kb on - strand, within EX31_RS16850 at 23.873 kb on - strand, within EX31_RS16850 at 23.933 kb on + strand, within EX31_RS16850 at 23.933 kb on + strand, within EX31_RS16850 at 23.933 kb on + strand, within EX31_RS16850 at 23.933 kb on + strand, within EX31_RS16850 at 23.933 kb on + strand, within EX31_RS16850 at 23.933 kb on + strand, within EX31_RS16850 at 23.934 kb on - strand, within EX31_RS16850 at 23.934 kb on - strand, within EX31_RS16850 at 23.934 kb on - strand, within EX31_RS16850 at 23.934 kb on - strand, within EX31_RS16850 at 23.934 kb on - strand, within EX31_RS16850 at 23.934 kb on - strand, within EX31_RS16850 at 23.934 kb on - strand, within EX31_RS16850 at 23.936 kb on - strand, within EX31_RS16850 at 23.942 kb on + strand, within EX31_RS16850 at 23.942 kb on + strand, within EX31_RS16850 at 23.942 kb on + strand, within EX31_RS16850 at 23.942 kb on + strand, within EX31_RS16850 at 23.943 kb on - strand, within EX31_RS16850 at 23.946 kb on - strand, within EX31_RS16850 at 23.946 kb on - strand, within EX31_RS16850 at 23.962 kb on + strand, within EX31_RS16850 at 23.962 kb on + strand, within EX31_RS16850 at 23.963 kb on - strand, within EX31_RS16850 at 23.963 kb on - strand, within EX31_RS16850 at 23.963 kb on - strand, within EX31_RS16850 at 23.963 kb on - strand, within EX31_RS16850 at 23.972 kb on + strand, within EX31_RS16850 at 24.070 kb on + strand, within EX31_RS16850 at 24.071 kb on - strand, within EX31_RS16850 at 24.076 kb on + strand, within EX31_RS16850 at 24.076 kb on + strand, within EX31_RS16850 at 24.077 kb on - strand, within EX31_RS16850 at 24.077 kb on - strand, within EX31_RS16850 at 24.134 kb on + strand, within EX31_RS16850 at 24.134 kb on + strand, within EX31_RS16850 at 24.134 kb on + strand, within EX31_RS16850 at 24.135 kb on - strand, within EX31_RS16850 at 24.136 kb on + strand, within EX31_RS16850 at 24.136 kb on + strand, within EX31_RS16850 at 24.136 kb on + strand, within EX31_RS16850 at 24.136 kb on + strand, within EX31_RS16850 at 24.137 kb on - strand, within EX31_RS16850 at 24.137 kb on - strand, within EX31_RS16850 at 24.137 kb on - strand, within EX31_RS16850 at 24.137 kb on - strand, within EX31_RS16850 at 24.137 kb on - strand, within EX31_RS16850 at 24.140 kb on + strand, within EX31_RS16850 at 24.140 kb on + strand, within EX31_RS16850 at 24.140 kb on + strand, within EX31_RS16850 at 24.140 kb on + strand, within EX31_RS16850 at 24.140 kb on + strand, within EX31_RS16850 at 24.140 kb on + strand, within EX31_RS16850 at 24.141 kb on - strand, within EX31_RS16850 at 24.141 kb on - strand, within EX31_RS16850 at 24.141 kb on - strand, within EX31_RS16850 at 24.141 kb on - strand, within EX31_RS16850 at 24.228 kb on + strand, within EX31_RS16850 at 24.228 kb on + strand, within EX31_RS16850 at 24.228 kb on + strand, within EX31_RS16850 at 24.310 kb on + strand, within EX31_RS16850 at 24.311 kb on - strand, within EX31_RS16850 at 24.311 kb on - strand, within EX31_RS16850 at 24.316 kb on + strand, within EX31_RS16850 at 24.432 kb on - strand at 24.455 kb on - strand at 24.469 kb on - strand at 24.469 kb on - strand at 24.485 kb on - strand at 24.485 kb on - strand at 24.582 kb on + strand at 24.635 kb on + strand at 24.635 kb on + strand at 24.635 kb on + strand at 24.635 kb on + strand at 24.635 kb on + strand at 24.635 kb on + strand at 24.635 kb on + strand at 24.635 kb on + strand at 24.635 kb on + strand at 24.635 kb on + strand at 24.635 kb on + strand at 24.635 kb on + strand at 24.635 kb on + strand at 24.636 kb on - strand at 24.636 kb on - strand at 24.636 kb on - strand at 24.636 kb on - strand at 24.636 kb on - strand at 24.636 kb on - strand at 24.636 kb on - strand at 24.636 kb on - strand at 24.636 kb on - strand at 24.636 kb on - strand at 24.646 kb on + strand at 24.646 kb on + strand at 24.647 kb on - strand at 24.647 kb on - strand at 24.694 kb on + strand at 24.694 kb on + strand at 24.694 kb on + strand at 24.694 kb on + strand at 24.695 kb on - strand at 24.695 kb on - strand at 24.695 kb on - strand at 24.695 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 22,448 + EX31_RS16835 0.79 +1.0 22,448 + EX31_RS16835 0.79 +0.6 22,448 + EX31_RS16835 0.79 +0.5 22,449 - EX31_RS16835 0.79 -2.2 22,449 - EX31_RS16835 0.79 +0.5 22,470 + EX31_RS16835 0.82 +0.5 22,489 + EX31_RS16835 0.85 +0.0 22,489 + EX31_RS16835 0.85 -1.8 22,490 - EX31_RS16835 0.85 +0.5 22,514 + EX31_RS16835 0.89 -1.8 22,607 + -0.7 22,607 + +0.5 22,616 - -0.2 22,664 + -1.1 22,664 + -1.4 22,664 + +0.2 22,664 + -1.5 22,665 - -0.3 22,665 - +0.1 22,665 - -1.6 22,665 - -0.6 22,668 + -0.9 22,668 + -0.9 22,668 + -0.6 22,669 - +0.1 22,682 + -0.8 22,682 + -0.2 22,682 + -1.8 22,682 + +0.8 22,682 + -1.2 22,748 - EX31_RS16840 0.15 +1.3 22,756 + EX31_RS16840 0.16 +0.5 22,757 - EX31_RS16840 0.16 +1.5 22,820 + EX31_RS16840 0.27 +0.2 22,841 + EX31_RS16840 0.31 -0.7 22,841 + EX31_RS16840 0.31 -0.4 22,912 + EX31_RS16840 0.43 +0.1 22,912 + EX31_RS16840 0.43 -0.5 22,913 - EX31_RS16840 0.43 -1.0 22,921 + EX31_RS16840 0.44 +1.0 22,921 + EX31_RS16840 0.44 -0.2 22,921 + EX31_RS16840 0.44 +0.7 22,921 + EX31_RS16840 0.44 +0.5 22,921 + EX31_RS16840 0.44 +0.5 22,921 + EX31_RS16840 0.44 +1.0 22,921 + EX31_RS16840 0.44 -0.3 22,922 - EX31_RS16840 0.44 +0.2 22,922 - EX31_RS16840 0.44 +1.6 22,950 + EX31_RS16840 0.49 +0.6 22,950 + EX31_RS16840 0.49 -2.1 22,950 + EX31_RS16840 0.49 +0.6 22,950 + EX31_RS16840 0.49 -1.5 22,950 + EX31_RS16840 0.49 +1.1 22,951 - EX31_RS16840 0.49 +0.2 22,958 + EX31_RS16840 0.50 +0.8 23,180 + EX31_RS16840 0.88 +0.2 23,180 + EX31_RS16840 0.88 +0.3 23,180 + EX31_RS16840 0.88 +0.3 23,180 + EX31_RS16840 0.88 -2.7 23,183 + EX31_RS16840 0.88 +2.5 23,183 + EX31_RS16840 0.88 -0.2 23,248 + +1.1 23,248 + +0.8 23,249 - +0.5 23,268 + +0.4 23,268 + -0.8 23,269 - -1.1 23,360 + -0.3 23,360 + +0.8 23,360 + -0.2 23,361 - -0.7 23,387 + +0.4 23,428 + -1.1 23,428 + -0.5 23,429 - +0.4 23,429 - +0.3 23,435 + -0.3 23,436 - -1.0 23,440 + -0.5 23,440 + -0.7 23,440 + +0.3 23,441 - -1.1 23,441 - -0.3 23,441 - -0.5 23,441 - -1.0 23,441 - -0.1 23,441 - -0.5 23,466 + -1.0 23,466 + -1.9 23,521 + EX31_RS16845 0.29 +1.0 23,521 + EX31_RS16845 0.29 -0.3 23,565 + EX31_RS16845 0.45 -1.6 23,565 + EX31_RS16845 0.45 -0.8 23,571 + EX31_RS16845 0.48 -1.2 23,571 + EX31_RS16845 0.48 -0.2 23,595 + EX31_RS16845 0.56 -0.9 23,595 + EX31_RS16845 0.56 -1.0 23,707 + +0.2 23,708 - +0.7 23,711 + +0.2 23,711 + -0.3 23,711 + +0.3 23,782 - -0.1 23,782 - +1.9 23,790 + +0.1 23,790 + +1.8 23,791 - -0.3 23,838 + EX31_RS16850 0.17 -0.3 23,838 + EX31_RS16850 0.17 +0.5 23,855 + EX31_RS16850 0.19 +0.5 23,855 + EX31_RS16850 0.19 -0.4 23,856 - EX31_RS16850 0.19 -1.1 23,873 - EX31_RS16850 0.22 +0.1 23,873 - EX31_RS16850 0.22 -0.4 23,933 + EX31_RS16850 0.30 -2.0 23,933 + EX31_RS16850 0.30 -0.6 23,933 + EX31_RS16850 0.30 -0.8 23,933 + EX31_RS16850 0.30 +0.6 23,933 + EX31_RS16850 0.30 +0.8 23,933 + EX31_RS16850 0.30 -0.7 23,934 - EX31_RS16850 0.30 +1.4 23,934 - EX31_RS16850 0.30 -0.3 23,934 - EX31_RS16850 0.30 -0.9 23,934 - EX31_RS16850 0.30 +0.0 23,934 - EX31_RS16850 0.30 -0.8 23,934 - EX31_RS16850 0.30 -0.5 23,934 - EX31_RS16850 0.30 -0.3 23,936 - EX31_RS16850 0.31 +1.2 23,942 + EX31_RS16850 0.31 +0.4 23,942 + EX31_RS16850 0.31 -0.3 23,942 + EX31_RS16850 0.31 +0.7 23,942 + EX31_RS16850 0.31 +0.4 23,943 - EX31_RS16850 0.32 -1.2 23,946 - EX31_RS16850 0.32 -0.4 23,946 - EX31_RS16850 0.32 +1.2 23,962 + EX31_RS16850 0.34 +0.5 23,962 + EX31_RS16850 0.34 -1.1 23,963 - EX31_RS16850 0.34 +0.5 23,963 - EX31_RS16850 0.34 +0.3 23,963 - EX31_RS16850 0.34 -0.5 23,963 - EX31_RS16850 0.34 -1.5 23,972 + EX31_RS16850 0.36 +1.0 24,070 + EX31_RS16850 0.50 -1.7 24,071 - EX31_RS16850 0.50 -1.9 24,076 + EX31_RS16850 0.51 -0.4 24,076 + EX31_RS16850 0.51 +1.6 24,077 - EX31_RS16850 0.51 -2.1 24,077 - EX31_RS16850 0.51 +1.2 24,134 + EX31_RS16850 0.59 +0.9 24,134 + EX31_RS16850 0.59 +1.6 24,134 + EX31_RS16850 0.59 +2.0 24,135 - EX31_RS16850 0.59 -0.4 24,136 + EX31_RS16850 0.59 +0.5 24,136 + EX31_RS16850 0.59 -1.4 24,136 + EX31_RS16850 0.59 -0.2 24,136 + EX31_RS16850 0.59 -1.0 24,137 - EX31_RS16850 0.59 +0.2 24,137 - EX31_RS16850 0.59 +1.3 24,137 - EX31_RS16850 0.59 +0.8 24,137 - EX31_RS16850 0.59 -0.6 24,137 - EX31_RS16850 0.59 -0.5 24,140 + EX31_RS16850 0.60 +1.9 24,140 + EX31_RS16850 0.60 +0.3 24,140 + EX31_RS16850 0.60 -0.3 24,140 + EX31_RS16850 0.60 +0.7 24,140 + EX31_RS16850 0.60 +1.1 24,140 + EX31_RS16850 0.60 +2.5 24,141 - EX31_RS16850 0.60 +0.4 24,141 - EX31_RS16850 0.60 +0.2 24,141 - EX31_RS16850 0.60 -1.6 24,141 - EX31_RS16850 0.60 +0.5 24,228 + EX31_RS16850 0.73 +0.5 24,228 + EX31_RS16850 0.73 -0.8 24,228 + EX31_RS16850 0.73 -0.0 24,310 + EX31_RS16850 0.84 +0.4 24,311 - EX31_RS16850 0.84 +1.8 24,311 - EX31_RS16850 0.84 +1.1 24,316 + EX31_RS16850 0.85 +0.4 24,432 - +1.5 24,455 - +0.8 24,469 - -1.0 24,469 - +0.9 24,485 - -0.2 24,485 - -0.1 24,582 + +0.0 24,635 + +0.2 24,635 + +1.4 24,635 + +0.1 24,635 + +1.1 24,635 + -1.1 24,635 + -0.5 24,635 + +2.3 24,635 + +1.2 24,635 + +0.2 24,635 + +0.9 24,635 + -0.3 24,635 + +0.0 24,635 + +0.3 24,636 - +0.5 24,636 - +0.3 24,636 - +1.3 24,636 - -0.5 24,636 - +0.4 24,636 - +0.3 24,636 - +0.3 24,636 - +0.5 24,636 - -0.3 24,636 - -0.3 24,646 + -0.2 24,646 + -0.2 24,647 - -0.1 24,647 - +0.1 24,694 + -1.4 24,694 + -1.8 24,694 + -1.4 24,694 + -1.0 24,695 - -2.1 24,695 - -1.8 24,695 - -3.1 24,695 - -0.3
Or see this region's nucleotide sequence