Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS13870 and EX31_RS13875 are separated by 293 nucleotides EX31_RS13875 and EX31_RS13880 are separated by 214 nucleotides
EX31_RS13870: EX31_RS13870 - pyruvate kinase PykF, at 32,961 to 34,373
_RS13870
EX31_RS13875: EX31_RS13875 - major outer membrane lipoprotein, at 34,667 to 34,903
_RS13875
EX31_RS13880: EX31_RS13880 - L,D-transpeptidase family protein, at 35,118 to 36,131
_RS13880
Position (kb) 34
35 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 33.853 kb on + strand, within EX31_RS13870 at 34.124 kb on + strand, within EX31_RS13870 at 34.128 kb on + strand, within EX31_RS13870 at 34.248 kb on + strand at 34.301 kb on + strand at 34.306 kb on + strand at 34.340 kb on + strand at 34.340 kb on + strand at 34.341 kb on - strand at 34.349 kb on + strand at 34.371 kb on + strand at 34.422 kb on + strand at 34.422 kb on + strand at 34.617 kb on + strand at 34.620 kb on + strand at 34.620 kb on + strand at 34.620 kb on + strand at 34.620 kb on + strand at 34.620 kb on + strand at 34.620 kb on + strand at 34.620 kb on + strand at 34.621 kb on - strand at 34.621 kb on - strand at 34.626 kb on + strand at 34.632 kb on + strand at 34.632 kb on + strand at 34.632 kb on + strand at 34.632 kb on + strand at 34.632 kb on + strand at 34.632 kb on + strand at 34.632 kb on + strand at 34.632 kb on + strand at 34.632 kb on + strand at 34.653 kb on + strand at 34.663 kb on + strand at 34.663 kb on + strand at 34.663 kb on + strand at 34.663 kb on + strand at 34.714 kb on + strand, within EX31_RS13875 at 34.714 kb on + strand, within EX31_RS13875 at 34.714 kb on + strand, within EX31_RS13875 at 34.714 kb on + strand, within EX31_RS13875 at 34.714 kb on + strand, within EX31_RS13875 at 34.714 kb on + strand, within EX31_RS13875 at 34.714 kb on + strand, within EX31_RS13875 at 34.714 kb on + strand, within EX31_RS13875 at 34.771 kb on + strand, within EX31_RS13875 at 34.783 kb on + strand, within EX31_RS13875 at 34.783 kb on + strand, within EX31_RS13875 at 34.783 kb on + strand, within EX31_RS13875 at 34.840 kb on + strand, within EX31_RS13875 at 34.892 kb on + strand at 34.892 kb on + strand at 34.901 kb on + strand at 34.904 kb on + strand at 34.904 kb on + strand at 34.908 kb on + strand at 34.908 kb on + strand at 34.908 kb on + strand at 34.908 kb on + strand at 34.916 kb on + strand at 34.916 kb on + strand at 34.916 kb on + strand at 34.916 kb on + strand at 35.022 kb on - strand at 35.089 kb on - strand at 35.119 kb on + strand at 35.246 kb on + strand, within EX31_RS13880 at 35.292 kb on - strand, within EX31_RS13880 at 35.292 kb on - strand, within EX31_RS13880 at 35.687 kb on + strand, within EX31_RS13880 at 35.765 kb on - strand, within EX31_RS13880 at 35.797 kb on + strand, within EX31_RS13880 at 35.798 kb on - strand, within EX31_RS13880 at 35.798 kb on - strand, within EX31_RS13880 at 35.798 kb on - strand, within EX31_RS13880 at 35.801 kb on - strand, within EX31_RS13880 at 35.832 kb on - strand, within EX31_RS13880 at 35.903 kb on - strand, within EX31_RS13880 at 35.903 kb on - strand, within EX31_RS13880 at 35.903 kb on - strand, within EX31_RS13880
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 33,853 + EX31_RS13870 0.63 +0.6 34,124 + EX31_RS13870 0.82 +0.3 34,128 + EX31_RS13870 0.83 -0.3 34,248 + -1.5 34,301 + +0.3 34,306 + +0.7 34,340 + +0.2 34,340 + -1.5 34,341 - +1.0 34,349 + +1.4 34,371 + -0.2 34,422 + -1.2 34,422 + +0.7 34,617 + -0.5 34,620 + -0.4 34,620 + +1.5 34,620 + -1.9 34,620 + -0.6 34,620 + -0.8 34,620 + -0.2 34,620 + -0.6 34,621 - -1.7 34,621 - -0.4 34,626 + -1.6 34,632 + -0.7 34,632 + -0.2 34,632 + -0.8 34,632 + -0.9 34,632 + -0.8 34,632 + -0.5 34,632 + -1.5 34,632 + -1.7 34,632 + -0.3 34,653 + -0.5 34,663 + -1.2 34,663 + +0.4 34,663 + -0.3 34,663 + +0.4 34,714 + EX31_RS13875 0.20 -0.9 34,714 + EX31_RS13875 0.20 -3.0 34,714 + EX31_RS13875 0.20 -0.6 34,714 + EX31_RS13875 0.20 -0.6 34,714 + EX31_RS13875 0.20 -0.9 34,714 + EX31_RS13875 0.20 -0.2 34,714 + EX31_RS13875 0.20 -0.8 34,714 + EX31_RS13875 0.20 -0.1 34,771 + EX31_RS13875 0.44 +0.7 34,783 + EX31_RS13875 0.49 -1.0 34,783 + EX31_RS13875 0.49 -1.1 34,783 + EX31_RS13875 0.49 -0.8 34,840 + EX31_RS13875 0.73 -0.7 34,892 + -1.5 34,892 + -0.9 34,901 + -1.0 34,904 + -0.6 34,904 + -0.4 34,908 + -1.0 34,908 + +0.2 34,908 + -0.9 34,908 + +0.2 34,916 + +1.1 34,916 + -1.3 34,916 + -0.6 34,916 + -0.3 35,022 - -0.5 35,089 - +2.3 35,119 + +0.3 35,246 + EX31_RS13880 0.13 +0.5 35,292 - EX31_RS13880 0.17 +0.6 35,292 - EX31_RS13880 0.17 +0.5 35,687 + EX31_RS13880 0.56 -0.1 35,765 - EX31_RS13880 0.64 +0.0 35,797 + EX31_RS13880 0.67 +2.1 35,798 - EX31_RS13880 0.67 -1.5 35,798 - EX31_RS13880 0.67 +0.6 35,798 - EX31_RS13880 0.67 -1.7 35,801 - EX31_RS13880 0.67 +0.6 35,832 - EX31_RS13880 0.70 -0.5 35,903 - EX31_RS13880 0.77 -1.1 35,903 - EX31_RS13880 0.77 +0.4 35,903 - EX31_RS13880 0.77 -0.8
Or see this region's nucleotide sequence