Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11280 and EX31_RS11285 are separated by 10 nucleotides EX31_RS11285 and EX31_RS11290 are separated by 127 nucleotides EX31_RS11290 and EX31_RS11295 are separated by 21 nucleotides
EX31_RS11280: EX31_RS11280 - flagellar basal body P-ring protein FlgI, at 189,142 to 190,248
_RS11280
EX31_RS11285: EX31_RS11285 - flagellar basal body L-ring protein FlgH, at 190,259 to 190,963
_RS11285
EX31_RS11290: EX31_RS11290 - flagellar basal-body rod protein FlgG, at 191,091 to 191,873
_RS11290
EX31_RS11295: EX31_RS11295 - flagellar basal body rod protein FlgF, at 191,895 to 192,650
_RS11295
Position (kb) 190
191 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 189.274 kb on + strand, within EX31_RS11280 at 189.552 kb on + strand, within EX31_RS11280 at 189.553 kb on - strand, within EX31_RS11280 at 189.669 kb on + strand, within EX31_RS11280 at 189.669 kb on + strand, within EX31_RS11280 at 189.705 kb on + strand, within EX31_RS11280 at 189.745 kb on - strand, within EX31_RS11280 at 189.766 kb on - strand, within EX31_RS11280 at 189.837 kb on - strand, within EX31_RS11280 at 189.903 kb on + strand, within EX31_RS11280 at 190.047 kb on + strand, within EX31_RS11280 at 190.048 kb on - strand, within EX31_RS11280 at 190.173 kb on + strand at 190.260 kb on + strand at 190.260 kb on + strand at 190.261 kb on - strand at 190.262 kb on + strand at 190.262 kb on + strand at 190.262 kb on + strand at 190.263 kb on - strand at 190.373 kb on - strand, within EX31_RS11285 at 190.582 kb on + strand, within EX31_RS11285 at 190.623 kb on + strand, within EX31_RS11285 at 190.624 kb on - strand, within EX31_RS11285 at 190.741 kb on + strand, within EX31_RS11285 at 190.742 kb on - strand, within EX31_RS11285 at 190.742 kb on - strand, within EX31_RS11285 at 190.742 kb on - strand, within EX31_RS11285 at 190.779 kb on + strand, within EX31_RS11285 at 190.780 kb on - strand, within EX31_RS11285 at 190.885 kb on + strand, within EX31_RS11285 at 190.886 kb on - strand, within EX31_RS11285 at 190.887 kb on + strand, within EX31_RS11285 at 190.887 kb on + strand, within EX31_RS11285 at 190.888 kb on - strand, within EX31_RS11285 at 190.888 kb on - strand, within EX31_RS11285 at 190.888 kb on - strand, within EX31_RS11285 at 190.888 kb on - strand, within EX31_RS11285 at 190.888 kb on - strand, within EX31_RS11285 at 190.888 kb on - strand, within EX31_RS11285 at 190.907 kb on + strand at 190.952 kb on - strand at 191.141 kb on + strand at 191.141 kb on + strand at 191.142 kb on - strand at 191.142 kb on - strand at 191.208 kb on - strand, within EX31_RS11290 at 191.221 kb on + strand, within EX31_RS11290 at 191.222 kb on - strand, within EX31_RS11290 at 191.222 kb on - strand, within EX31_RS11290 at 191.231 kb on - strand, within EX31_RS11290 at 191.534 kb on - strand, within EX31_RS11290 at 191.534 kb on - strand, within EX31_RS11290 at 191.534 kb on - strand, within EX31_RS11290 at 191.645 kb on + strand, within EX31_RS11290 at 191.646 kb on - strand, within EX31_RS11290 at 191.771 kb on + strand, within EX31_RS11290 at 191.772 kb on - strand, within EX31_RS11290 at 191.811 kb on + strand at 191.812 kb on - strand at 191.812 kb on - strand at 191.856 kb on - strand at 191.857 kb on + strand at 191.857 kb on + strand at 191.857 kb on + strand at 191.858 kb on - strand at 191.858 kb on - strand at 191.858 kb on - strand at 191.858 kb on - strand at 191.858 kb on - strand at 191.858 kb on - strand at 191.858 kb on - strand at 191.858 kb on - strand at 191.858 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 189,274 + EX31_RS11280 0.12 -0.7 189,552 + EX31_RS11280 0.37 +1.8 189,553 - EX31_RS11280 0.37 +0.3 189,669 + EX31_RS11280 0.48 +1.1 189,669 + EX31_RS11280 0.48 -1.7 189,705 + EX31_RS11280 0.51 +0.5 189,745 - EX31_RS11280 0.54 +1.8 189,766 - EX31_RS11280 0.56 +0.6 189,837 - EX31_RS11280 0.63 +2.4 189,903 + EX31_RS11280 0.69 +1.6 190,047 + EX31_RS11280 0.82 -1.5 190,048 - EX31_RS11280 0.82 +1.2 190,173 + +0.1 190,260 + -0.5 190,260 + -0.4 190,261 - -1.8 190,262 + +0.3 190,262 + +0.4 190,262 + -1.8 190,263 - -0.4 190,373 - EX31_RS11285 0.16 -0.2 190,582 + EX31_RS11285 0.46 +0.1 190,623 + EX31_RS11285 0.52 +2.6 190,624 - EX31_RS11285 0.52 -0.7 190,741 + EX31_RS11285 0.68 +0.6 190,742 - EX31_RS11285 0.69 -0.2 190,742 - EX31_RS11285 0.69 +3.6 190,742 - EX31_RS11285 0.69 +0.1 190,779 + EX31_RS11285 0.74 +0.7 190,780 - EX31_RS11285 0.74 -0.4 190,885 + EX31_RS11285 0.89 +0.7 190,886 - EX31_RS11285 0.89 +0.7 190,887 + EX31_RS11285 0.89 +0.5 190,887 + EX31_RS11285 0.89 +0.5 190,888 - EX31_RS11285 0.89 +0.3 190,888 - EX31_RS11285 0.89 +1.0 190,888 - EX31_RS11285 0.89 -0.4 190,888 - EX31_RS11285 0.89 +2.3 190,888 - EX31_RS11285 0.89 +2.1 190,888 - EX31_RS11285 0.89 -1.1 190,907 + -0.4 190,952 - +1.0 191,141 + +0.1 191,141 + -0.3 191,142 - -0.0 191,142 - -1.5 191,208 - EX31_RS11290 0.15 -0.1 191,221 + EX31_RS11290 0.17 +0.4 191,222 - EX31_RS11290 0.17 +0.6 191,222 - EX31_RS11290 0.17 -0.3 191,231 - EX31_RS11290 0.18 -0.1 191,534 - EX31_RS11290 0.57 -0.2 191,534 - EX31_RS11290 0.57 +1.0 191,534 - EX31_RS11290 0.57 +0.1 191,645 + EX31_RS11290 0.71 -0.7 191,646 - EX31_RS11290 0.71 -0.4 191,771 + EX31_RS11290 0.87 -0.9 191,772 - EX31_RS11290 0.87 +1.2 191,811 + +2.4 191,812 - +0.0 191,812 - +0.5 191,856 - +1.0 191,857 + +0.5 191,857 + +0.3 191,857 + -1.2 191,858 - -0.1 191,858 - +2.3 191,858 - -2.1 191,858 - -0.2 191,858 - -0.8 191,858 - +0.5 191,858 - +1.0 191,858 - -0.4 191,858 - +1.1
Or see this region's nucleotide sequence