Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10770 and EX31_RS10775 are separated by 80 nucleotides EX31_RS10775 and EX31_RS10780 are separated by 261 nucleotides
EX31_RS10770: EX31_RS10770 - HlyD family secretion protein, at 71,536 to 72,660
_RS10770
EX31_RS10775: EX31_RS10775 - DHA2 family efflux MFS transporter permease subunit, at 72,741 to 74,294
_RS10775
EX31_RS10780: EX31_RS10780 - L,D-transpeptidase family protein, at 74,556 to 75,476
_RS10780
Position (kb) 72
73
74
75 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 71.835 kb on - strand, within EX31_RS10770 at 71.835 kb on - strand, within EX31_RS10770 at 71.835 kb on - strand, within EX31_RS10770 at 71.835 kb on - strand, within EX31_RS10770 at 71.835 kb on - strand, within EX31_RS10770 at 71.881 kb on - strand, within EX31_RS10770 at 71.885 kb on + strand, within EX31_RS10770 at 71.885 kb on + strand, within EX31_RS10770 at 71.885 kb on + strand, within EX31_RS10770 at 71.886 kb on - strand, within EX31_RS10770 at 71.886 kb on - strand, within EX31_RS10770 at 71.886 kb on - strand, within EX31_RS10770 at 71.886 kb on - strand, within EX31_RS10770 at 71.886 kb on - strand, within EX31_RS10770 at 71.899 kb on + strand, within EX31_RS10770 at 71.900 kb on - strand, within EX31_RS10770 at 71.918 kb on - strand, within EX31_RS10770 at 71.941 kb on - strand, within EX31_RS10770 at 72.132 kb on + strand, within EX31_RS10770 at 72.135 kb on - strand, within EX31_RS10770 at 72.150 kb on + strand, within EX31_RS10770 at 72.151 kb on - strand, within EX31_RS10770 at 72.151 kb on - strand, within EX31_RS10770 at 72.415 kb on + strand, within EX31_RS10770 at 72.415 kb on + strand, within EX31_RS10770 at 72.416 kb on - strand, within EX31_RS10770 at 72.416 kb on - strand, within EX31_RS10770 at 72.461 kb on - strand, within EX31_RS10770 at 72.498 kb on - strand, within EX31_RS10770 at 72.498 kb on - strand, within EX31_RS10770 at 72.617 kb on - strand at 72.833 kb on + strand at 72.833 kb on + strand at 72.834 kb on - strand at 72.940 kb on - strand, within EX31_RS10775 at 73.247 kb on - strand, within EX31_RS10775 at 73.247 kb on - strand, within EX31_RS10775 at 73.247 kb on - strand, within EX31_RS10775 at 73.259 kb on - strand, within EX31_RS10775 at 73.332 kb on - strand, within EX31_RS10775 at 73.332 kb on - strand, within EX31_RS10775 at 73.365 kb on - strand, within EX31_RS10775 at 73.387 kb on + strand, within EX31_RS10775 at 73.387 kb on + strand, within EX31_RS10775 at 73.422 kb on + strand, within EX31_RS10775 at 73.422 kb on + strand, within EX31_RS10775 at 73.423 kb on - strand, within EX31_RS10775 at 73.423 kb on - strand, within EX31_RS10775 at 73.423 kb on - strand, within EX31_RS10775 at 73.549 kb on + strand, within EX31_RS10775 at 73.631 kb on + strand, within EX31_RS10775 at 73.631 kb on + strand, within EX31_RS10775 at 73.631 kb on + strand, within EX31_RS10775 at 73.631 kb on + strand, within EX31_RS10775 at 73.631 kb on + strand, within EX31_RS10775 at 73.632 kb on - strand, within EX31_RS10775 at 73.632 kb on - strand, within EX31_RS10775 at 73.635 kb on + strand, within EX31_RS10775 at 73.636 kb on - strand, within EX31_RS10775 at 73.636 kb on - strand, within EX31_RS10775 at 73.636 kb on - strand, within EX31_RS10775 at 73.636 kb on - strand, within EX31_RS10775 at 73.691 kb on + strand, within EX31_RS10775 at 73.691 kb on + strand, within EX31_RS10775 at 73.692 kb on - strand, within EX31_RS10775 at 73.813 kb on + strand, within EX31_RS10775 at 73.892 kb on + strand, within EX31_RS10775 at 73.892 kb on + strand, within EX31_RS10775 at 73.892 kb on + strand, within EX31_RS10775 at 73.892 kb on + strand, within EX31_RS10775 at 73.892 kb on + strand, within EX31_RS10775 at 73.893 kb on - strand, within EX31_RS10775 at 73.893 kb on - strand, within EX31_RS10775 at 74.022 kb on - strand, within EX31_RS10775 at 74.270 kb on + strand at 74.292 kb on + strand at 74.292 kb on + strand at 74.293 kb on - strand at 74.393 kb on + strand at 74.401 kb on + strand at 74.404 kb on - strand at 74.407 kb on + strand at 74.407 kb on + strand at 74.408 kb on - strand at 74.437 kb on + strand at 74.458 kb on + strand at 74.458 kb on + strand at 74.458 kb on + strand at 74.458 kb on + strand at 74.458 kb on + strand at 74.458 kb on + strand at 74.458 kb on + strand at 74.458 kb on + strand at 74.458 kb on + strand at 74.458 kb on + strand at 74.458 kb on + strand at 74.458 kb on + strand at 74.458 kb on + strand at 74.459 kb on - strand at 74.459 kb on - strand at 74.459 kb on - strand at 74.468 kb on + strand at 74.468 kb on + strand at 74.468 kb on + strand at 74.468 kb on + strand at 74.469 kb on - strand at 74.469 kb on - strand at 74.469 kb on - strand at 74.473 kb on + strand at 74.473 kb on + strand at 74.473 kb on + strand at 74.473 kb on + strand at 74.473 kb on + strand at 74.607 kb on + strand at 74.607 kb on + strand at 74.607 kb on + strand at 74.607 kb on + strand at 74.607 kb on + strand at 74.608 kb on - strand at 74.609 kb on + strand at 74.609 kb on + strand at 74.609 kb on + strand at 74.609 kb on + strand at 74.634 kb on + strand at 74.684 kb on + strand, within EX31_RS10780 at 74.684 kb on + strand, within EX31_RS10780 at 74.684 kb on + strand, within EX31_RS10780 at 74.684 kb on + strand, within EX31_RS10780 at 74.684 kb on + strand, within EX31_RS10780 at 74.684 kb on + strand, within EX31_RS10780 at 74.685 kb on - strand, within EX31_RS10780 at 74.685 kb on - strand, within EX31_RS10780 at 74.765 kb on + strand, within EX31_RS10780 at 74.765 kb on + strand, within EX31_RS10780 at 74.886 kb on + strand, within EX31_RS10780 at 74.886 kb on + strand, within EX31_RS10780 at 74.886 kb on + strand, within EX31_RS10780 at 74.886 kb on + strand, within EX31_RS10780 at 74.887 kb on - strand, within EX31_RS10780 at 74.889 kb on + strand, within EX31_RS10780 at 74.889 kb on + strand, within EX31_RS10780 at 74.889 kb on + strand, within EX31_RS10780 at 74.890 kb on - strand, within EX31_RS10780 at 74.890 kb on - strand, within EX31_RS10780 at 74.890 kb on - strand, within EX31_RS10780 at 74.909 kb on + strand, within EX31_RS10780 at 74.909 kb on + strand, within EX31_RS10780 at 74.909 kb on + strand, within EX31_RS10780 at 74.987 kb on + strand, within EX31_RS10780 at 75.036 kb on + strand, within EX31_RS10780 at 75.036 kb on + strand, within EX31_RS10780 at 75.036 kb on + strand, within EX31_RS10780 at 75.111 kb on + strand, within EX31_RS10780 at 75.111 kb on + strand, within EX31_RS10780 at 75.111 kb on + strand, within EX31_RS10780 at 75.111 kb on + strand, within EX31_RS10780 at 75.111 kb on + strand, within EX31_RS10780 at 75.111 kb on + strand, within EX31_RS10780 at 75.111 kb on + strand, within EX31_RS10780 at 75.126 kb on + strand, within EX31_RS10780 at 75.126 kb on + strand, within EX31_RS10780
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 71,835 - EX31_RS10770 0.27 -0.9 71,835 - EX31_RS10770 0.27 -0.4 71,835 - EX31_RS10770 0.27 -3.4 71,835 - EX31_RS10770 0.27 -1.6 71,835 - EX31_RS10770 0.27 +0.4 71,881 - EX31_RS10770 0.31 +1.1 71,885 + EX31_RS10770 0.31 +2.8 71,885 + EX31_RS10770 0.31 -1.5 71,885 + EX31_RS10770 0.31 -0.2 71,886 - EX31_RS10770 0.31 +0.4 71,886 - EX31_RS10770 0.31 -0.7 71,886 - EX31_RS10770 0.31 +0.5 71,886 - EX31_RS10770 0.31 +0.6 71,886 - EX31_RS10770 0.31 +0.5 71,899 + EX31_RS10770 0.32 +0.6 71,900 - EX31_RS10770 0.32 -0.4 71,918 - EX31_RS10770 0.34 +0.3 71,941 - EX31_RS10770 0.36 +0.7 72,132 + EX31_RS10770 0.53 +1.6 72,135 - EX31_RS10770 0.53 -0.3 72,150 + EX31_RS10770 0.55 -0.3 72,151 - EX31_RS10770 0.55 +2.2 72,151 - EX31_RS10770 0.55 -0.8 72,415 + EX31_RS10770 0.78 +0.4 72,415 + EX31_RS10770 0.78 -1.8 72,416 - EX31_RS10770 0.78 +0.2 72,416 - EX31_RS10770 0.78 +0.6 72,461 - EX31_RS10770 0.82 +1.1 72,498 - EX31_RS10770 0.86 -1.2 72,498 - EX31_RS10770 0.86 -0.7 72,617 - -0.3 72,833 + -0.0 72,833 + +0.1 72,834 - -0.2 72,940 - EX31_RS10775 0.13 +0.2 73,247 - EX31_RS10775 0.33 -0.9 73,247 - EX31_RS10775 0.33 -1.2 73,247 - EX31_RS10775 0.33 -0.1 73,259 - EX31_RS10775 0.33 +0.6 73,332 - EX31_RS10775 0.38 -0.4 73,332 - EX31_RS10775 0.38 +1.1 73,365 - EX31_RS10775 0.40 +0.7 73,387 + EX31_RS10775 0.42 +0.4 73,387 + EX31_RS10775 0.42 -0.1 73,422 + EX31_RS10775 0.44 +1.5 73,422 + EX31_RS10775 0.44 -0.1 73,423 - EX31_RS10775 0.44 -1.1 73,423 - EX31_RS10775 0.44 +0.1 73,423 - EX31_RS10775 0.44 -0.5 73,549 + EX31_RS10775 0.52 +0.6 73,631 + EX31_RS10775 0.57 -0.8 73,631 + EX31_RS10775 0.57 +0.5 73,631 + EX31_RS10775 0.57 +0.2 73,631 + EX31_RS10775 0.57 +0.4 73,631 + EX31_RS10775 0.57 +2.6 73,632 - EX31_RS10775 0.57 +0.2 73,632 - EX31_RS10775 0.57 +2.0 73,635 + EX31_RS10775 0.58 -1.9 73,636 - EX31_RS10775 0.58 +0.1 73,636 - EX31_RS10775 0.58 +0.1 73,636 - EX31_RS10775 0.58 +0.4 73,636 - EX31_RS10775 0.58 +2.4 73,691 + EX31_RS10775 0.61 -0.7 73,691 + EX31_RS10775 0.61 +2.6 73,692 - EX31_RS10775 0.61 +0.2 73,813 + EX31_RS10775 0.69 +0.9 73,892 + EX31_RS10775 0.74 +0.7 73,892 + EX31_RS10775 0.74 -1.5 73,892 + EX31_RS10775 0.74 +1.1 73,892 + EX31_RS10775 0.74 +0.8 73,892 + EX31_RS10775 0.74 -0.9 73,893 - EX31_RS10775 0.74 -0.4 73,893 - EX31_RS10775 0.74 +1.4 74,022 - EX31_RS10775 0.82 +0.6 74,270 + +0.7 74,292 + +0.7 74,292 + -0.0 74,293 - -0.5 74,393 + -0.5 74,401 + +1.1 74,404 - -0.4 74,407 + +0.4 74,407 + +0.2 74,408 - +0.2 74,437 + -0.4 74,458 + -0.0 74,458 + +0.9 74,458 + -0.2 74,458 + +1.0 74,458 + +0.2 74,458 + +2.3 74,458 + -0.4 74,458 + +1.4 74,458 + -1.7 74,458 + +0.4 74,458 + -0.6 74,458 + +1.3 74,458 + +0.9 74,459 - +1.1 74,459 - -1.2 74,459 - -1.5 74,468 + +0.1 74,468 + -1.6 74,468 + +0.4 74,468 + -0.4 74,469 - +0.2 74,469 - -0.5 74,469 - +1.0 74,473 + -0.5 74,473 + +0.6 74,473 + +0.1 74,473 + -0.7 74,473 + -0.8 74,607 + +0.5 74,607 + -1.0 74,607 + -0.4 74,607 + -0.8 74,607 + +0.4 74,608 - -1.4 74,609 + -1.4 74,609 + -0.4 74,609 + -0.4 74,609 + +0.3 74,634 + -1.0 74,684 + EX31_RS10780 0.14 +1.1 74,684 + EX31_RS10780 0.14 -1.4 74,684 + EX31_RS10780 0.14 -0.9 74,684 + EX31_RS10780 0.14 -1.8 74,684 + EX31_RS10780 0.14 +0.6 74,684 + EX31_RS10780 0.14 -0.8 74,685 - EX31_RS10780 0.14 -0.9 74,685 - EX31_RS10780 0.14 -0.9 74,765 + EX31_RS10780 0.23 -0.9 74,765 + EX31_RS10780 0.23 +0.6 74,886 + EX31_RS10780 0.36 +0.2 74,886 + EX31_RS10780 0.36 -0.4 74,886 + EX31_RS10780 0.36 -2.2 74,886 + EX31_RS10780 0.36 -0.8 74,887 - EX31_RS10780 0.36 -1.2 74,889 + EX31_RS10780 0.36 +0.6 74,889 + EX31_RS10780 0.36 -0.9 74,889 + EX31_RS10780 0.36 +0.4 74,890 - EX31_RS10780 0.36 +1.9 74,890 - EX31_RS10780 0.36 +1.1 74,890 - EX31_RS10780 0.36 +0.1 74,909 + EX31_RS10780 0.38 +0.9 74,909 + EX31_RS10780 0.38 -1.4 74,909 + EX31_RS10780 0.38 +0.1 74,987 + EX31_RS10780 0.47 -0.5 75,036 + EX31_RS10780 0.52 -1.5 75,036 + EX31_RS10780 0.52 +0.1 75,036 + EX31_RS10780 0.52 +0.2 75,111 + EX31_RS10780 0.60 -1.2 75,111 + EX31_RS10780 0.60 +0.8 75,111 + EX31_RS10780 0.60 -1.0 75,111 + EX31_RS10780 0.60 -0.4 75,111 + EX31_RS10780 0.60 +0.8 75,111 + EX31_RS10780 0.60 +0.3 75,111 + EX31_RS10780 0.60 -1.1 75,126 + EX31_RS10780 0.62 -0.7 75,126 + EX31_RS10780 0.62 -0.1
Or see this region's nucleotide sequence