Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03310 and EX31_RS03315 are separated by 384 nucleotides EX31_RS03315 and EX31_RS03320 are separated by 1 nucleotides EX31_RS03320 and EX31_RS03325 overlap by 4 nucleotides
EX31_RS03310: EX31_RS03310 - hypothetical protein, at 152,918 to 153,250
_RS03310
EX31_RS03315: EX31_RS03315 - type VI secretion system tube protein Hcp, at 153,635 to 154,126
_RS03315
EX31_RS03320: EX31_RS03320 - hypothetical protein, at 154,128 to 154,628
_RS03320
EX31_RS03325: EX31_RS03325 - DUF2778 domain-containing protein, at 154,625 to 154,984
_RS03325
Position (kb) 153
154
155 Strain fitness (log2 ratio) -2
-1
0
1
2 at 152.689 kb on + strand at 152.690 kb on - strand at 152.690 kb on - strand at 152.690 kb on - strand at 152.760 kb on + strand at 152.812 kb on - strand at 152.841 kb on + strand at 152.888 kb on - strand at 152.947 kb on + strand at 152.947 kb on + strand at 152.947 kb on + strand at 153.026 kb on + strand, within EX31_RS03310 at 153.119 kb on + strand, within EX31_RS03310 at 153.190 kb on - strand, within EX31_RS03310 at 153.214 kb on + strand, within EX31_RS03310 at 153.215 kb on - strand, within EX31_RS03310 at 153.267 kb on + strand at 153.272 kb on + strand at 153.288 kb on + strand at 153.327 kb on - strand at 153.467 kb on + strand at 153.554 kb on - strand at 153.591 kb on + strand at 153.592 kb on - strand at 153.632 kb on + strand at 153.633 kb on - strand at 153.636 kb on + strand at 153.637 kb on - strand at 153.676 kb on + strand at 153.677 kb on - strand at 153.817 kb on + strand, within EX31_RS03315 at 153.818 kb on - strand, within EX31_RS03315 at 153.818 kb on - strand, within EX31_RS03315 at 153.907 kb on + strand, within EX31_RS03315 at 153.908 kb on - strand, within EX31_RS03315 at 153.916 kb on - strand, within EX31_RS03315 at 154.007 kb on - strand, within EX31_RS03315 at 154.010 kb on + strand, within EX31_RS03315 at 154.066 kb on + strand, within EX31_RS03315 at 154.066 kb on + strand, within EX31_RS03315 at 154.081 kb on + strand at 154.081 kb on + strand at 154.108 kb on + strand at 154.130 kb on - strand at 154.130 kb on - strand at 154.175 kb on + strand at 154.178 kb on + strand, within EX31_RS03320 at 154.208 kb on + strand, within EX31_RS03320 at 154.235 kb on + strand, within EX31_RS03320 at 154.235 kb on + strand, within EX31_RS03320 at 154.236 kb on - strand, within EX31_RS03320 at 154.236 kb on - strand, within EX31_RS03320 at 154.237 kb on + strand, within EX31_RS03320 at 154.237 kb on + strand, within EX31_RS03320 at 154.238 kb on - strand, within EX31_RS03320 at 154.238 kb on - strand, within EX31_RS03320 at 154.267 kb on + strand, within EX31_RS03320 at 154.267 kb on + strand, within EX31_RS03320 at 154.267 kb on + strand, within EX31_RS03320 at 154.268 kb on - strand, within EX31_RS03320 at 154.268 kb on - strand, within EX31_RS03320 at 154.284 kb on + strand, within EX31_RS03320 at 154.306 kb on - strand, within EX31_RS03320 at 154.320 kb on - strand, within EX31_RS03320 at 154.346 kb on + strand, within EX31_RS03320 at 154.347 kb on - strand, within EX31_RS03320 at 154.374 kb on - strand, within EX31_RS03320 at 154.456 kb on - strand, within EX31_RS03320 at 154.471 kb on + strand, within EX31_RS03320 at 154.471 kb on + strand, within EX31_RS03320 at 154.471 kb on + strand, within EX31_RS03320 at 154.471 kb on + strand, within EX31_RS03320 at 154.471 kb on + strand, within EX31_RS03320 at 154.472 kb on - strand, within EX31_RS03320 at 154.472 kb on - strand, within EX31_RS03320 at 154.472 kb on - strand, within EX31_RS03320 at 154.472 kb on - strand, within EX31_RS03320 at 154.472 kb on - strand, within EX31_RS03320 at 154.472 kb on - strand, within EX31_RS03320 at 154.472 kb on - strand, within EX31_RS03320 at 154.472 kb on - strand, within EX31_RS03320 at 154.498 kb on + strand, within EX31_RS03320 at 154.564 kb on - strand, within EX31_RS03320 at 154.768 kb on + strand, within EX31_RS03325 at 154.769 kb on - strand, within EX31_RS03325 at 154.769 kb on - strand, within EX31_RS03325 at 154.835 kb on - strand, within EX31_RS03325 at 154.895 kb on + strand, within EX31_RS03325 at 154.916 kb on - strand, within EX31_RS03325 at 155.089 kb on - strand at 155.092 kb on + strand at 155.093 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 152,689 + -0.4 152,690 - +0.1 152,690 - +0.8 152,690 - +0.6 152,760 + +0.3 152,812 - -1.3 152,841 + -0.8 152,888 - +2.4 152,947 + +0.4 152,947 + +1.4 152,947 + +0.3 153,026 + EX31_RS03310 0.32 -1.7 153,119 + EX31_RS03310 0.60 -0.9 153,190 - EX31_RS03310 0.82 -1.0 153,214 + EX31_RS03310 0.89 -1.2 153,215 - EX31_RS03310 0.89 +0.8 153,267 + -1.0 153,272 + +0.8 153,288 + +0.5 153,327 - -0.4 153,467 + +1.1 153,554 - +0.4 153,591 + +1.8 153,592 - -1.1 153,632 + -0.0 153,633 - +0.5 153,636 + -0.6 153,637 - +1.2 153,676 + +0.3 153,677 - +1.2 153,817 + EX31_RS03315 0.37 -1.9 153,818 - EX31_RS03315 0.37 -0.2 153,818 - EX31_RS03315 0.37 -0.8 153,907 + EX31_RS03315 0.55 +0.2 153,908 - EX31_RS03315 0.55 -0.6 153,916 - EX31_RS03315 0.57 -2.3 154,007 - EX31_RS03315 0.76 +2.7 154,010 + EX31_RS03315 0.76 +0.1 154,066 + EX31_RS03315 0.88 -0.7 154,066 + EX31_RS03315 0.88 +0.3 154,081 + +0.2 154,081 + -0.0 154,108 + -0.5 154,130 - +0.6 154,130 - -2.1 154,175 + -0.3 154,178 + EX31_RS03320 0.10 -0.9 154,208 + EX31_RS03320 0.16 +1.6 154,235 + EX31_RS03320 0.21 +1.2 154,235 + EX31_RS03320 0.21 -1.4 154,236 - EX31_RS03320 0.22 +0.2 154,236 - EX31_RS03320 0.22 -1.4 154,237 + EX31_RS03320 0.22 +0.3 154,237 + EX31_RS03320 0.22 +0.7 154,238 - EX31_RS03320 0.22 +0.4 154,238 - EX31_RS03320 0.22 +1.1 154,267 + EX31_RS03320 0.28 -0.0 154,267 + EX31_RS03320 0.28 -0.1 154,267 + EX31_RS03320 0.28 -1.6 154,268 - EX31_RS03320 0.28 +1.4 154,268 - EX31_RS03320 0.28 +1.9 154,284 + EX31_RS03320 0.31 +0.7 154,306 - EX31_RS03320 0.36 -0.1 154,320 - EX31_RS03320 0.38 -1.2 154,346 + EX31_RS03320 0.44 +0.9 154,347 - EX31_RS03320 0.44 +0.3 154,374 - EX31_RS03320 0.49 +1.1 154,456 - EX31_RS03320 0.65 +1.1 154,471 + EX31_RS03320 0.68 -1.0 154,471 + EX31_RS03320 0.68 -1.7 154,471 + EX31_RS03320 0.68 +0.1 154,471 + EX31_RS03320 0.68 +0.3 154,471 + EX31_RS03320 0.68 -2.0 154,472 - EX31_RS03320 0.69 +1.0 154,472 - EX31_RS03320 0.69 -1.1 154,472 - EX31_RS03320 0.69 -0.5 154,472 - EX31_RS03320 0.69 -0.3 154,472 - EX31_RS03320 0.69 -0.3 154,472 - EX31_RS03320 0.69 +0.2 154,472 - EX31_RS03320 0.69 -0.3 154,472 - EX31_RS03320 0.69 +0.1 154,498 + EX31_RS03320 0.74 +0.5 154,564 - EX31_RS03320 0.87 -1.0 154,768 + EX31_RS03325 0.40 +0.9 154,769 - EX31_RS03325 0.40 -0.6 154,769 - EX31_RS03325 0.40 +1.1 154,835 - EX31_RS03325 0.58 -0.0 154,895 + EX31_RS03325 0.75 -0.9 154,916 - EX31_RS03325 0.81 -0.0 155,089 - +1.7 155,092 + +1.5 155,093 - -0.2
Or see this region's nucleotide sequence