Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01950 and EX31_RS01955 overlap by 4 nucleotides EX31_RS01955 and EX31_RS01960 are separated by 39 nucleotides EX31_RS01960 and EX31_RS01965 are separated by 39 nucleotides EX31_RS01965 and EX31_RS01970 are separated by 195 nucleotides
EX31_RS01950: EX31_RS01950 - DUF2235 domain-containing protein, at 427,940 to 429,523
_RS01950
EX31_RS01955: EX31_RS01955 - DUF2931 family protein, at 429,520 to 430,167
_RS01955
EX31_RS01960: EX31_RS01960 - DUF2931 family protein, at 430,207 to 430,854
_RS01960
EX31_RS01965: EX31_RS01965 - DUF2931 family protein, at 430,894 to 431,544
_RS01965
EX31_RS01970: EX31_RS01970 - hypothetical protein, at 431,740 to 432,225
_RS01970
Position (kb) 430
431 Strain fitness (log2 ratio) -1
0
1
2
3 at 429.289 kb on + strand, within EX31_RS01950 at 429.291 kb on + strand, within EX31_RS01950 at 429.330 kb on + strand, within EX31_RS01950 at 429.347 kb on + strand, within EX31_RS01950 at 429.347 kb on + strand, within EX31_RS01950 at 429.347 kb on + strand, within EX31_RS01950 at 429.381 kb on + strand at 429.385 kb on + strand at 429.577 kb on - strand at 429.636 kb on + strand, within EX31_RS01955 at 429.636 kb on + strand, within EX31_RS01955 at 429.697 kb on + strand, within EX31_RS01955 at 429.704 kb on - strand, within EX31_RS01955 at 429.834 kb on + strand, within EX31_RS01955 at 429.867 kb on - strand, within EX31_RS01955 at 430.030 kb on - strand, within EX31_RS01955 at 430.287 kb on + strand, within EX31_RS01960 at 430.405 kb on - strand, within EX31_RS01960 at 430.448 kb on - strand, within EX31_RS01960 at 430.474 kb on - strand, within EX31_RS01960 at 430.628 kb on - strand, within EX31_RS01960 at 430.755 kb on + strand, within EX31_RS01960 at 430.756 kb on - strand, within EX31_RS01960 at 430.871 kb on - strand at 430.875 kb on - strand at 431.003 kb on - strand, within EX31_RS01965 at 431.013 kb on + strand, within EX31_RS01965 at 431.014 kb on - strand, within EX31_RS01965 at 431.064 kb on + strand, within EX31_RS01965 at 431.164 kb on - strand, within EX31_RS01965 at 431.334 kb on - strand, within EX31_RS01965 at 431.337 kb on - strand, within EX31_RS01965 at 431.381 kb on - strand, within EX31_RS01965 at 431.400 kb on - strand, within EX31_RS01965
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 429,289 + EX31_RS01950 0.85 +3.5 429,291 + EX31_RS01950 0.85 +1.0 429,330 + EX31_RS01950 0.88 -1.4 429,347 + EX31_RS01950 0.89 +0.3 429,347 + EX31_RS01950 0.89 +0.6 429,347 + EX31_RS01950 0.89 +1.9 429,381 + -0.3 429,385 + -0.6 429,577 - +0.8 429,636 + EX31_RS01955 0.18 +1.2 429,636 + EX31_RS01955 0.18 +0.6 429,697 + EX31_RS01955 0.27 +0.3 429,704 - EX31_RS01955 0.28 +0.9 429,834 + EX31_RS01955 0.48 +0.3 429,867 - EX31_RS01955 0.54 +1.3 430,030 - EX31_RS01955 0.79 -0.4 430,287 + EX31_RS01960 0.12 -0.8 430,405 - EX31_RS01960 0.31 -1.3 430,448 - EX31_RS01960 0.37 -0.3 430,474 - EX31_RS01960 0.41 -0.3 430,628 - EX31_RS01960 0.65 -0.1 430,755 + EX31_RS01960 0.85 -0.1 430,756 - EX31_RS01960 0.85 -0.8 430,871 - +0.8 430,875 - +1.9 431,003 - EX31_RS01965 0.17 -0.3 431,013 + EX31_RS01965 0.18 -0.8 431,014 - EX31_RS01965 0.18 +0.7 431,064 + EX31_RS01965 0.26 -0.3 431,164 - EX31_RS01965 0.41 -0.5 431,334 - EX31_RS01965 0.68 +0.9 431,337 - EX31_RS01965 0.68 +2.9 431,381 - EX31_RS01965 0.75 +0.4 431,400 - EX31_RS01965 0.78 +1.4
Or see this region's nucleotide sequence