Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01740 and EX31_RS01745 are separated by 108 nucleotides EX31_RS01745 and EX31_RS01750 are separated by 103 nucleotides EX31_RS01750 and EX31_RS01755 are separated by 3 nucleotides EX31_RS01755 and EX31_RS01760 overlap by 1 nucleotides
EX31_RS01740: EX31_RS01740 - helix-turn-helix domain-containing protein, at 378,017 to 378,256
_RS01740
EX31_RS01745: EX31_RS01745 - helix-turn-helix transcriptional regulator, at 378,365 to 379,084
_RS01745
EX31_RS01750: EX31_RS01750 - hexulose-6-phosphate synthase, at 379,188 to 379,430
_RS01750
EX31_RS01755: EX31_RS01755 - type II toxin-antitoxin system HicB family antitoxin, at 379,434 to 379,892
_RS01755
EX31_RS01760: EX31_RS01760 - hypothetical protein, at 379,892 to 380,287
_RS01760
Position (kb)
379
380 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 378.341 kb on + strand at 378.341 kb on + strand at 378.351 kb on + strand at 378.351 kb on + strand at 378.353 kb on + strand at 379.085 kb on + strand at 379.112 kb on + strand at 379.112 kb on + strand at 379.112 kb on + strand at 379.112 kb on + strand at 379.112 kb on + strand at 379.113 kb on - strand at 379.131 kb on + strand at 379.131 kb on + strand at 379.131 kb on + strand at 379.131 kb on + strand at 379.132 kb on - strand at 379.168 kb on + strand at 379.168 kb on + strand at 379.168 kb on + strand at 379.168 kb on + strand at 379.172 kb on + strand at 379.180 kb on + strand at 379.180 kb on + strand at 379.180 kb on + strand at 379.180 kb on + strand at 379.180 kb on + strand at 379.229 kb on - strand, within EX31_RS01750 at 379.232 kb on + strand, within EX31_RS01750 at 379.258 kb on + strand, within EX31_RS01750 at 379.275 kb on + strand, within EX31_RS01750 at 379.275 kb on + strand, within EX31_RS01750 at 379.275 kb on + strand, within EX31_RS01750 at 379.323 kb on + strand, within EX31_RS01750 at 379.325 kb on + strand, within EX31_RS01750 at 379.340 kb on + strand, within EX31_RS01750 at 379.340 kb on + strand, within EX31_RS01750 at 379.389 kb on + strand, within EX31_RS01750 at 379.389 kb on + strand, within EX31_RS01750 at 379.389 kb on + strand, within EX31_RS01750 at 379.396 kb on + strand, within EX31_RS01750 at 379.396 kb on + strand, within EX31_RS01750 at 379.649 kb on + strand, within EX31_RS01755 at 379.655 kb on + strand, within EX31_RS01755 at 379.692 kb on + strand, within EX31_RS01755 at 379.715 kb on + strand, within EX31_RS01755 at 379.715 kb on + strand, within EX31_RS01755 at 379.715 kb on + strand, within EX31_RS01755 at 379.754 kb on + strand, within EX31_RS01755 at 379.787 kb on + strand, within EX31_RS01755 at 379.789 kb on + strand, within EX31_RS01755 at 379.972 kb on + strand, within EX31_RS01760 at 379.972 kb on + strand, within EX31_RS01760 at 379.972 kb on + strand, within EX31_RS01760 at 380.049 kb on + strand, within EX31_RS01760 at 380.094 kb on + strand, within EX31_RS01760 at 380.104 kb on + strand, within EX31_RS01760 at 380.115 kb on + strand, within EX31_RS01760 at 380.201 kb on + strand, within EX31_RS01760 at 380.205 kb on + strand, within EX31_RS01760 at 380.205 kb on + strand, within EX31_RS01760 at 380.205 kb on + strand, within EX31_RS01760 at 380.205 kb on + strand, within EX31_RS01760 at 380.205 kb on + strand, within EX31_RS01760 at 380.205 kb on + strand, within EX31_RS01760 at 380.205 kb on + strand, within EX31_RS01760 at 380.205 kb on + strand, within EX31_RS01760 at 380.205 kb on + strand, within EX31_RS01760 at 380.205 kb on + strand, within EX31_RS01760 at 380.245 kb on + strand, within EX31_RS01760 at 380.245 kb on + strand, within EX31_RS01760 at 380.326 kb on + strand at 380.326 kb on + strand at 380.326 kb on + strand at 380.326 kb on + strand at 380.327 kb on - strand at 380.327 kb on - strand at 380.327 kb on - strand at 380.341 kb on + strand at 380.379 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 378,341 + -1.3 378,341 + +0.6 378,351 + +0.6 378,351 + -0.3 378,353 + +0.5 379,085 + -0.4 379,112 + -0.4 379,112 + +0.2 379,112 + +0.7 379,112 + +1.1 379,112 + -1.6 379,113 - +0.3 379,131 + +3.5 379,131 + -0.2 379,131 + -0.9 379,131 + +0.3 379,132 - -0.2 379,168 + -0.0 379,168 + -0.0 379,168 + +1.5 379,168 + -2.3 379,172 + -0.1 379,180 + +1.2 379,180 + -1.2 379,180 + -1.0 379,180 + -0.1 379,180 + -0.4 379,229 - EX31_RS01750 0.17 +2.2 379,232 + EX31_RS01750 0.18 +1.7 379,258 + EX31_RS01750 0.29 -0.5 379,275 + EX31_RS01750 0.36 +1.5 379,275 + EX31_RS01750 0.36 +0.9 379,275 + EX31_RS01750 0.36 +0.3 379,323 + EX31_RS01750 0.56 +2.0 379,325 + EX31_RS01750 0.56 +1.1 379,340 + EX31_RS01750 0.63 +0.3 379,340 + EX31_RS01750 0.63 +0.2 379,389 + EX31_RS01750 0.83 +0.9 379,389 + EX31_RS01750 0.83 +1.4 379,389 + EX31_RS01750 0.83 -0.1 379,396 + EX31_RS01750 0.86 +1.1 379,396 + EX31_RS01750 0.86 +0.5 379,649 + EX31_RS01755 0.47 +1.6 379,655 + EX31_RS01755 0.48 -0.3 379,692 + EX31_RS01755 0.56 +0.5 379,715 + EX31_RS01755 0.61 +0.0 379,715 + EX31_RS01755 0.61 +0.0 379,715 + EX31_RS01755 0.61 +0.1 379,754 + EX31_RS01755 0.70 -0.7 379,787 + EX31_RS01755 0.77 -1.4 379,789 + EX31_RS01755 0.77 -0.1 379,972 + EX31_RS01760 0.20 +0.2 379,972 + EX31_RS01760 0.20 +1.1 379,972 + EX31_RS01760 0.20 +0.3 380,049 + EX31_RS01760 0.40 +0.5 380,094 + EX31_RS01760 0.51 +0.9 380,104 + EX31_RS01760 0.54 +0.7 380,115 + EX31_RS01760 0.56 +0.1 380,201 + EX31_RS01760 0.78 +0.2 380,205 + EX31_RS01760 0.79 -0.3 380,205 + EX31_RS01760 0.79 +1.1 380,205 + EX31_RS01760 0.79 +2.1 380,205 + EX31_RS01760 0.79 -0.1 380,205 + EX31_RS01760 0.79 +0.1 380,205 + EX31_RS01760 0.79 +1.9 380,205 + EX31_RS01760 0.79 +0.2 380,205 + EX31_RS01760 0.79 +0.0 380,205 + EX31_RS01760 0.79 +0.3 380,205 + EX31_RS01760 0.79 +0.6 380,245 + EX31_RS01760 0.89 +0.0 380,245 + EX31_RS01760 0.89 +1.0 380,326 + +0.7 380,326 + +0.7 380,326 + +0.8 380,326 + -0.9 380,327 - -2.3 380,327 - -1.4 380,327 - +1.1 380,341 + -1.6 380,379 - +0.8
Or see this region's nucleotide sequence