Strain Fitness in Methanococcus maripaludis S2 around MMP_RS03450

Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse2; 5 generations

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS03435 and MMP_RS03440 are separated by 57 nucleotidesMMP_RS03440 and MMP_RS03445 are separated by 75 nucleotidesMMP_RS03445 and MMP_RS03450 are separated by 74 nucleotidesMMP_RS03450 and MMP_RS03455 are separated by 97 nucleotides MMP_RS03435: MMP_RS03435 - ketol-acid reductoisomerase, at 645,729 to 646,721 _RS03435 MMP_RS03440: MMP_RS03440 - MoaD/ThiS family protein, at 646,779 to 646,973 _RS03440 MMP_RS03445: MMP_RS03445 - methanogenesis marker 17 protein, at 647,049 to 647,600 _RS03445 MMP_RS03450: MMP_RS03450 - glycine synthesis protein GlyXS (from data), at 647,675 to 647,947 _RS03450 MMP_RS03455: MMP_RS03455 - radical SAM protein, at 648,045 to 648,938 _RS03455 Position (kb) 647 648Strain fitness (log2 ratio) -2 -1 0 1at 646.720 kb on + strandat 646.870 kb on - strand, within MMP_RS03440at 646.979 kb on - strandat 646.983 kb on - strandat 646.984 kb on + strandat 646.987 kb on - strandat 646.997 kb on + strandat 647.001 kb on - strandat 647.002 kb on + strandat 647.002 kb on + strandat 647.004 kb on + strandat 647.018 kb on - strandat 647.055 kb on - strandat 647.407 kb on - strand, within MMP_RS03445at 647.695 kb on - strandat 647.797 kb on - strand, within MMP_RS03450at 647.797 kb on - strand, within MMP_RS03450at 647.852 kb on - strand, within MMP_RS03450at 647.951 kb on - strandat 647.951 kb on - strandat 648.008 kb on - strandat 648.008 kb on - strandat 648.009 kb on + strandat 648.015 kb on - strandat 648.086 kb on - strandat 648.087 kb on + strandat 648.117 kb on - strandat 648.186 kb on + strandat 648.261 kb on - strandat 648.262 kb on + strandat 648.262 kb on + strandat 648.508 kb on + strandat 648.718 kb on - strandat 648.902 kb on - strandat 648.903 kb on + strandat 648.905 kb on - strandat 648.927 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse2; 5 generations
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646,720 + +0.0
646,870 - MMP_RS03440 0.47 +0.2
646,979 - -0.4
646,983 - +0.0
646,984 + -0.1
646,987 - -0.2
646,997 + -0.5
647,001 - -1.9
647,002 + -0.2
647,002 + -0.2
647,004 + +0.1
647,018 - -1.4
647,055 - -1.2
647,407 - MMP_RS03445 0.65 -1.3
647,695 - -0.0
647,797 - MMP_RS03450 0.45 +0.5
647,797 - MMP_RS03450 0.45 +0.3
647,852 - MMP_RS03450 0.65 +0.1
647,951 - -0.5
647,951 - +1.0
648,008 - +1.0
648,008 - +0.5
648,009 + +0.8
648,015 - +0.7
648,086 - +0.1
648,087 + -0.2
648,117 - -0.7
648,186 + -2.6
648,261 - -0.8
648,262 + -0.9
648,262 + -1.9
648,508 + -1.9
648,718 - -2.3
648,902 - -1.2
648,903 + -1.6
648,905 - -0.9
648,927 + -0.2

Or see this region's nucleotide sequence