Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS08860

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS08850 and MMJJ_RS08855 are separated by 18 nucleotidesMMJJ_RS08855 and MMJJ_RS08860 are separated by 109 nucleotidesMMJJ_RS08860 and MMJJ_RS08865 overlap by 4 nucleotidesMMJJ_RS08865 and MMJJ_RS08870 are separated by 131 nucleotides MMJJ_RS08850: MMJJ_RS08850 - succinate dehydrogenase/fumarate reductase iron-sulfur subunit, at 1,631,417 to 1,632,901 _RS08850 MMJJ_RS08855: MMJJ_RS08855 - cyclic pyranopterin monophosphate synthase MoaC, at 1,632,920 to 1,633,381 _RS08855 MMJJ_RS08860: MMJJ_RS08860 - Rrf2 family transcriptional regulator, at 1,633,491 to 1,633,925 _RS08860 MMJJ_RS08865: MMJJ_RS08865 - class IV adenylate cyclase, at 1,633,922 to 1,634,485 _RS08865 MMJJ_RS08870: MMJJ_RS08870 - 2-isopropylmalate synthase, at 1,634,617 to 1,636,161 _RS08870 Position (kb) 1633 1634Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1632.597 kb on - strand, within MMJJ_RS08850at 1632.752 kb on - strand, within MMJJ_RS08850at 1632.752 kb on - strand, within MMJJ_RS08850at 1632.784 kb on + strandat 1632.800 kb on - strandat 1632.899 kb on - strandat 1633.008 kb on + strand, within MMJJ_RS08855at 1633.050 kb on + strand, within MMJJ_RS08855at 1633.139 kb on - strand, within MMJJ_RS08855at 1633.241 kb on - strand, within MMJJ_RS08855at 1633.244 kb on + strand, within MMJJ_RS08855at 1633.376 kb on + strandat 1633.376 kb on + strandat 1633.382 kb on - strandat 1633.384 kb on - strandat 1633.385 kb on + strandat 1633.415 kb on + strandat 1633.421 kb on + strandat 1633.432 kb on - strandat 1633.438 kb on - strandat 1633.439 kb on + strandat 1633.442 kb on - strandat 1633.442 kb on - strandat 1633.442 kb on - strandat 1633.442 kb on - strandat 1633.442 kb on - strandat 1633.443 kb on + strandat 1633.443 kb on + strandat 1633.443 kb on + strandat 1633.443 kb on + strandat 1633.443 kb on + strandat 1633.457 kb on + strandat 1633.460 kb on - strandat 1633.460 kb on - strandat 1633.461 kb on + strandat 1633.461 kb on + strandat 1633.461 kb on + strandat 1633.475 kb on + strandat 1633.509 kb on + strandat 1633.519 kb on - strandat 1633.519 kb on - strandat 1633.523 kb on - strandat 1633.595 kb on - strand, within MMJJ_RS08860at 1633.599 kb on - strand, within MMJJ_RS08860at 1633.599 kb on - strand, within MMJJ_RS08860at 1633.599 kb on - strand, within MMJJ_RS08860at 1633.600 kb on + strand, within MMJJ_RS08860at 1633.602 kb on + strand, within MMJJ_RS08860at 1633.638 kb on + strand, within MMJJ_RS08860at 1633.684 kb on + strand, within MMJJ_RS08860at 1633.715 kb on - strand, within MMJJ_RS08860at 1633.716 kb on + strand, within MMJJ_RS08860at 1633.733 kb on + strandat 1633.832 kb on - strand, within MMJJ_RS08860at 1633.838 kb on + strand, within MMJJ_RS08860at 1633.908 kb on + strandat 1633.922 kb on + strandat 1633.945 kb on - strandat 1633.945 kb on - strandat 1633.951 kb on + strandat 1634.036 kb on - strand, within MMJJ_RS08865at 1634.037 kb on + strand, within MMJJ_RS08865at 1634.050 kb on + strand, within MMJJ_RS08865at 1634.127 kb on - strand, within MMJJ_RS08865at 1634.128 kb on + strand, within MMJJ_RS08865at 1634.128 kb on + strand, within MMJJ_RS08865at 1634.129 kb on - strand, within MMJJ_RS08865at 1634.129 kb on - strand, within MMJJ_RS08865at 1634.129 kb on - strand, within MMJJ_RS08865at 1634.129 kb on - strand, within MMJJ_RS08865at 1634.129 kb on - strand, within MMJJ_RS08865at 1634.130 kb on + strand, within MMJJ_RS08865at 1634.130 kb on + strand, within MMJJ_RS08865at 1634.130 kb on + strand, within MMJJ_RS08865at 1634.130 kb on + strand, within MMJJ_RS08865at 1634.130 kb on + strand, within MMJJ_RS08865at 1634.138 kb on - strand, within MMJJ_RS08865at 1634.138 kb on - strand, within MMJJ_RS08865at 1634.139 kb on + strand, within MMJJ_RS08865at 1634.139 kb on + strand, within MMJJ_RS08865at 1634.139 kb on + strand, within MMJJ_RS08865at 1634.211 kb on - strand, within MMJJ_RS08865at 1634.212 kb on + strand, within MMJJ_RS08865at 1634.212 kb on + strand, within MMJJ_RS08865at 1634.217 kb on + strand, within MMJJ_RS08865at 1634.217 kb on + strand, within MMJJ_RS08865at 1634.222 kb on + strand, within MMJJ_RS08865at 1634.356 kb on + strand, within MMJJ_RS08865at 1634.451 kb on + strandat 1634.454 kb on - strandat 1634.521 kb on - strandat 1634.521 kb on - strandat 1634.521 kb on - strandat 1634.522 kb on + strandat 1634.522 kb on + strandat 1634.522 kb on + strandat 1634.590 kb on - strandat 1634.595 kb on - strandat 1634.626 kb on - strandat 1634.685 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
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1,632,597 - MMJJ_RS08850 0.79 -0.1
1,632,752 - MMJJ_RS08850 0.90 -1.7
1,632,752 - MMJJ_RS08850 0.90 -2.5
1,632,784 + -1.7
1,632,800 - -3.3
1,632,899 - +2.2
1,633,008 + MMJJ_RS08855 0.19 -2.0
1,633,050 + MMJJ_RS08855 0.28 -2.0
1,633,139 - MMJJ_RS08855 0.47 -3.6
1,633,241 - MMJJ_RS08855 0.69 -3.8
1,633,244 + MMJJ_RS08855 0.70 -2.7
1,633,376 + -0.5
1,633,376 + -2.5
1,633,382 - -1.9
1,633,384 - -2.9
1,633,385 + -0.9
1,633,415 + -0.6
1,633,421 + -1.1
1,633,432 - +0.2
1,633,438 - -1.5
1,633,439 + -1.7
1,633,442 - -1.3
1,633,442 - -2.5
1,633,442 - +0.1
1,633,442 - -1.0
1,633,442 - -1.7
1,633,443 + -2.7
1,633,443 + -0.1
1,633,443 + -2.5
1,633,443 + -0.1
1,633,443 + -0.1
1,633,457 + -3.3
1,633,460 - -1.7
1,633,460 - +0.2
1,633,461 + -0.9
1,633,461 + -1.1
1,633,461 + -1.0
1,633,475 + -0.6
1,633,509 + -0.6
1,633,519 - -0.4
1,633,519 - +1.2
1,633,523 - +0.2
1,633,595 - MMJJ_RS08860 0.24 +0.2
1,633,599 - MMJJ_RS08860 0.25 -1.3
1,633,599 - MMJJ_RS08860 0.25 +0.1
1,633,599 - MMJJ_RS08860 0.25 -1.2
1,633,600 + MMJJ_RS08860 0.25 -0.0
1,633,602 + MMJJ_RS08860 0.26 -1.1
1,633,638 + MMJJ_RS08860 0.34 -0.1
1,633,684 + MMJJ_RS08860 0.44 -0.7
1,633,715 - MMJJ_RS08860 0.51 -0.8
1,633,716 + MMJJ_RS08860 0.52 -1.2
1,633,733 + +0.5
1,633,832 - MMJJ_RS08860 0.78 +0.1
1,633,838 + MMJJ_RS08860 0.80 +0.3
1,633,908 + -0.3
1,633,922 + -0.1
1,633,945 - -2.7
1,633,945 - +0.5
1,633,951 + +1.5
1,634,036 - MMJJ_RS08865 0.20 +0.6
1,634,037 + MMJJ_RS08865 0.20 -1.0
1,634,050 + MMJJ_RS08865 0.23 +1.5
1,634,127 - MMJJ_RS08865 0.36 -1.4
1,634,128 + MMJJ_RS08865 0.37 +1.8
1,634,128 + MMJJ_RS08865 0.37 +0.3
1,634,129 - MMJJ_RS08865 0.37 -0.7
1,634,129 - MMJJ_RS08865 0.37 +0.3
1,634,129 - MMJJ_RS08865 0.37 -0.4
1,634,129 - MMJJ_RS08865 0.37 -0.7
1,634,129 - MMJJ_RS08865 0.37 +0.6
1,634,130 + MMJJ_RS08865 0.37 -0.9
1,634,130 + MMJJ_RS08865 0.37 -1.0
1,634,130 + MMJJ_RS08865 0.37 -1.1
1,634,130 + MMJJ_RS08865 0.37 +1.5
1,634,130 + MMJJ_RS08865 0.37 -0.5
1,634,138 - MMJJ_RS08865 0.38 -1.1
1,634,138 - MMJJ_RS08865 0.38 +0.7
1,634,139 + MMJJ_RS08865 0.38 -0.0
1,634,139 + MMJJ_RS08865 0.38 +0.0
1,634,139 + MMJJ_RS08865 0.38 -1.0
1,634,211 - MMJJ_RS08865 0.51 -0.7
1,634,212 + MMJJ_RS08865 0.51 -1.5
1,634,212 + MMJJ_RS08865 0.51 -2.1
1,634,217 + MMJJ_RS08865 0.52 -0.5
1,634,217 + MMJJ_RS08865 0.52 +0.1
1,634,222 + MMJJ_RS08865 0.53 -1.0
1,634,356 + MMJJ_RS08865 0.77 -0.4
1,634,451 + -0.1
1,634,454 - -0.4
1,634,521 - +0.7
1,634,521 - -1.3
1,634,521 - -0.7
1,634,522 + +0.7
1,634,522 + -0.2
1,634,522 + -0.4
1,634,590 - -0.5
1,634,595 - +0.3
1,634,626 - -2.7
1,634,685 - -2.7

Or see this region's nucleotide sequence