Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08850 and MMJJ_RS08855 are separated by 18 nucleotides MMJJ_RS08855 and MMJJ_RS08860 are separated by 109 nucleotides MMJJ_RS08860 and MMJJ_RS08865 overlap by 4 nucleotides MMJJ_RS08865 and MMJJ_RS08870 are separated by 131 nucleotides
MMJJ_RS08850: MMJJ_RS08850 - succinate dehydrogenase/fumarate reductase iron-sulfur subunit, at 1,631,417 to 1,632,901
_RS08850
MMJJ_RS08855: MMJJ_RS08855 - cyclic pyranopterin monophosphate synthase MoaC, at 1,632,920 to 1,633,381
_RS08855
MMJJ_RS08860: MMJJ_RS08860 - Rrf2 family transcriptional regulator, at 1,633,491 to 1,633,925
_RS08860
MMJJ_RS08865: MMJJ_RS08865 - class IV adenylate cyclase, at 1,633,922 to 1,634,485
_RS08865
MMJJ_RS08870: MMJJ_RS08870 - 2-isopropylmalate synthase, at 1,634,617 to 1,636,161
_RS08870
Position (kb)
1633
1634 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1632.597 kb on - strand, within MMJJ_RS08850 at 1632.752 kb on - strand, within MMJJ_RS08850 at 1632.752 kb on - strand, within MMJJ_RS08850 at 1632.784 kb on + strand at 1632.800 kb on - strand at 1632.899 kb on - strand at 1633.008 kb on + strand, within MMJJ_RS08855 at 1633.050 kb on + strand, within MMJJ_RS08855 at 1633.139 kb on - strand, within MMJJ_RS08855 at 1633.241 kb on - strand, within MMJJ_RS08855 at 1633.244 kb on + strand, within MMJJ_RS08855 at 1633.376 kb on + strand at 1633.376 kb on + strand at 1633.382 kb on - strand at 1633.384 kb on - strand at 1633.385 kb on + strand at 1633.415 kb on + strand at 1633.421 kb on + strand at 1633.432 kb on - strand at 1633.438 kb on - strand at 1633.439 kb on + strand at 1633.442 kb on - strand at 1633.442 kb on - strand at 1633.442 kb on - strand at 1633.442 kb on - strand at 1633.442 kb on - strand at 1633.443 kb on + strand at 1633.443 kb on + strand at 1633.443 kb on + strand at 1633.443 kb on + strand at 1633.443 kb on + strand at 1633.457 kb on + strand at 1633.460 kb on - strand at 1633.460 kb on - strand at 1633.461 kb on + strand at 1633.461 kb on + strand at 1633.461 kb on + strand at 1633.475 kb on + strand at 1633.509 kb on + strand at 1633.519 kb on - strand at 1633.519 kb on - strand at 1633.523 kb on - strand at 1633.595 kb on - strand, within MMJJ_RS08860 at 1633.599 kb on - strand, within MMJJ_RS08860 at 1633.599 kb on - strand, within MMJJ_RS08860 at 1633.599 kb on - strand, within MMJJ_RS08860 at 1633.600 kb on + strand, within MMJJ_RS08860 at 1633.602 kb on + strand, within MMJJ_RS08860 at 1633.638 kb on + strand, within MMJJ_RS08860 at 1633.684 kb on + strand, within MMJJ_RS08860 at 1633.715 kb on - strand, within MMJJ_RS08860 at 1633.716 kb on + strand, within MMJJ_RS08860 at 1633.733 kb on + strand at 1633.832 kb on - strand, within MMJJ_RS08860 at 1633.838 kb on + strand, within MMJJ_RS08860 at 1633.908 kb on + strand at 1633.922 kb on + strand at 1633.945 kb on - strand at 1633.945 kb on - strand at 1633.951 kb on + strand at 1634.036 kb on - strand, within MMJJ_RS08865 at 1634.037 kb on + strand, within MMJJ_RS08865 at 1634.050 kb on + strand, within MMJJ_RS08865 at 1634.127 kb on - strand, within MMJJ_RS08865 at 1634.128 kb on + strand, within MMJJ_RS08865 at 1634.128 kb on + strand, within MMJJ_RS08865 at 1634.129 kb on - strand, within MMJJ_RS08865 at 1634.129 kb on - strand, within MMJJ_RS08865 at 1634.129 kb on - strand, within MMJJ_RS08865 at 1634.129 kb on - strand, within MMJJ_RS08865 at 1634.129 kb on - strand, within MMJJ_RS08865 at 1634.130 kb on + strand, within MMJJ_RS08865 at 1634.130 kb on + strand, within MMJJ_RS08865 at 1634.130 kb on + strand, within MMJJ_RS08865 at 1634.130 kb on + strand, within MMJJ_RS08865 at 1634.130 kb on + strand, within MMJJ_RS08865 at 1634.138 kb on - strand, within MMJJ_RS08865 at 1634.138 kb on - strand, within MMJJ_RS08865 at 1634.139 kb on + strand, within MMJJ_RS08865 at 1634.139 kb on + strand, within MMJJ_RS08865 at 1634.139 kb on + strand, within MMJJ_RS08865 at 1634.211 kb on - strand, within MMJJ_RS08865 at 1634.212 kb on + strand, within MMJJ_RS08865 at 1634.212 kb on + strand, within MMJJ_RS08865 at 1634.217 kb on + strand, within MMJJ_RS08865 at 1634.217 kb on + strand, within MMJJ_RS08865 at 1634.222 kb on + strand, within MMJJ_RS08865 at 1634.356 kb on + strand, within MMJJ_RS08865 at 1634.451 kb on + strand at 1634.454 kb on - strand at 1634.521 kb on - strand at 1634.521 kb on - strand at 1634.521 kb on - strand at 1634.522 kb on + strand at 1634.522 kb on + strand at 1634.522 kb on + strand at 1634.590 kb on - strand at 1634.595 kb on - strand at 1634.626 kb on - strand at 1634.685 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE remove 1,632,597 - MMJJ_RS08850 0.79 -0.1 1,632,752 - MMJJ_RS08850 0.90 -1.7 1,632,752 - MMJJ_RS08850 0.90 -2.5 1,632,784 + -1.7 1,632,800 - -3.3 1,632,899 - +2.2 1,633,008 + MMJJ_RS08855 0.19 -2.0 1,633,050 + MMJJ_RS08855 0.28 -2.0 1,633,139 - MMJJ_RS08855 0.47 -3.6 1,633,241 - MMJJ_RS08855 0.69 -3.8 1,633,244 + MMJJ_RS08855 0.70 -2.7 1,633,376 + -0.5 1,633,376 + -2.5 1,633,382 - -1.9 1,633,384 - -2.9 1,633,385 + -0.9 1,633,415 + -0.6 1,633,421 + -1.1 1,633,432 - +0.2 1,633,438 - -1.5 1,633,439 + -1.7 1,633,442 - -1.3 1,633,442 - -2.5 1,633,442 - +0.1 1,633,442 - -1.0 1,633,442 - -1.7 1,633,443 + -2.7 1,633,443 + -0.1 1,633,443 + -2.5 1,633,443 + -0.1 1,633,443 + -0.1 1,633,457 + -3.3 1,633,460 - -1.7 1,633,460 - +0.2 1,633,461 + -0.9 1,633,461 + -1.1 1,633,461 + -1.0 1,633,475 + -0.6 1,633,509 + -0.6 1,633,519 - -0.4 1,633,519 - +1.2 1,633,523 - +0.2 1,633,595 - MMJJ_RS08860 0.24 +0.2 1,633,599 - MMJJ_RS08860 0.25 -1.3 1,633,599 - MMJJ_RS08860 0.25 +0.1 1,633,599 - MMJJ_RS08860 0.25 -1.2 1,633,600 + MMJJ_RS08860 0.25 -0.0 1,633,602 + MMJJ_RS08860 0.26 -1.1 1,633,638 + MMJJ_RS08860 0.34 -0.1 1,633,684 + MMJJ_RS08860 0.44 -0.7 1,633,715 - MMJJ_RS08860 0.51 -0.8 1,633,716 + MMJJ_RS08860 0.52 -1.2 1,633,733 + +0.5 1,633,832 - MMJJ_RS08860 0.78 +0.1 1,633,838 + MMJJ_RS08860 0.80 +0.3 1,633,908 + -0.3 1,633,922 + -0.1 1,633,945 - -2.7 1,633,945 - +0.5 1,633,951 + +1.5 1,634,036 - MMJJ_RS08865 0.20 +0.6 1,634,037 + MMJJ_RS08865 0.20 -1.0 1,634,050 + MMJJ_RS08865 0.23 +1.5 1,634,127 - MMJJ_RS08865 0.36 -1.4 1,634,128 + MMJJ_RS08865 0.37 +1.8 1,634,128 + MMJJ_RS08865 0.37 +0.3 1,634,129 - MMJJ_RS08865 0.37 -0.7 1,634,129 - MMJJ_RS08865 0.37 +0.3 1,634,129 - MMJJ_RS08865 0.37 -0.4 1,634,129 - MMJJ_RS08865 0.37 -0.7 1,634,129 - MMJJ_RS08865 0.37 +0.6 1,634,130 + MMJJ_RS08865 0.37 -0.9 1,634,130 + MMJJ_RS08865 0.37 -1.0 1,634,130 + MMJJ_RS08865 0.37 -1.1 1,634,130 + MMJJ_RS08865 0.37 +1.5 1,634,130 + MMJJ_RS08865 0.37 -0.5 1,634,138 - MMJJ_RS08865 0.38 -1.1 1,634,138 - MMJJ_RS08865 0.38 +0.7 1,634,139 + MMJJ_RS08865 0.38 -0.0 1,634,139 + MMJJ_RS08865 0.38 +0.0 1,634,139 + MMJJ_RS08865 0.38 -1.0 1,634,211 - MMJJ_RS08865 0.51 -0.7 1,634,212 + MMJJ_RS08865 0.51 -1.5 1,634,212 + MMJJ_RS08865 0.51 -2.1 1,634,217 + MMJJ_RS08865 0.52 -0.5 1,634,217 + MMJJ_RS08865 0.52 +0.1 1,634,222 + MMJJ_RS08865 0.53 -1.0 1,634,356 + MMJJ_RS08865 0.77 -0.4 1,634,451 + -0.1 1,634,454 - -0.4 1,634,521 - +0.7 1,634,521 - -1.3 1,634,521 - -0.7 1,634,522 + +0.7 1,634,522 + -0.2 1,634,522 + -0.4 1,634,590 - -0.5 1,634,595 - +0.3 1,634,626 - -2.7 1,634,685 - -2.7
Or see this region's nucleotide sequence