Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06075 and MMJJ_RS06080 are separated by 111 nucleotides MMJJ_RS06080 and MMJJ_RS06085 are separated by 23 nucleotides MMJJ_RS06085 and MMJJ_RS06090 are separated by 19 nucleotides MMJJ_RS06090 and MMJJ_RS06095 are separated by 69 nucleotides
MMJJ_RS06075: MMJJ_RS06075 - DNA-binding protein Alba, at 1,128,838 to 1,129,107
_RS06075
MMJJ_RS06080: MMJJ_RS06080 - serine/threonine protein kinase, at 1,129,219 to 1,129,896
_RS06080
MMJJ_RS06085: MMJJ_RS06085 - proteasome assembly chaperone family protein, at 1,129,920 to 1,130,672
_RS06085
MMJJ_RS06090: MMJJ_RS06090 - hypothetical protein, at 1,130,692 to 1,131,366
_RS06090
MMJJ_RS06095: MMJJ_RS06095 - formylmethanofuran--tetrahydromethanopterin N-formyltransferase, at 1,131,436 to 1,132,344
_RS06095
Position (kb)
1129
1130
1131 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1129.009 kb on - strand, within MMJJ_RS06075 at 1129.039 kb on + strand, within MMJJ_RS06075 at 1129.048 kb on + strand, within MMJJ_RS06075 at 1129.064 kb on - strand, within MMJJ_RS06075 at 1129.064 kb on - strand, within MMJJ_RS06075 at 1129.073 kb on + strand, within MMJJ_RS06075 at 1129.091 kb on - strand at 1129.092 kb on + strand at 1129.098 kb on - strand at 1129.131 kb on - strand at 1129.132 kb on + strand at 1129.145 kb on - strand at 1129.145 kb on - strand at 1129.145 kb on - strand at 1129.145 kb on - strand at 1129.146 kb on + strand at 1129.146 kb on + strand at 1129.147 kb on - strand at 1129.147 kb on - strand at 1129.147 kb on - strand at 1129.148 kb on + strand at 1129.148 kb on + strand at 1129.149 kb on - strand at 1129.149 kb on - strand at 1129.149 kb on - strand at 1129.150 kb on + strand at 1129.162 kb on + strand at 1129.195 kb on + strand at 1129.195 kb on + strand at 1129.257 kb on - strand at 1129.312 kb on - strand, within MMJJ_RS06080 at 1129.330 kb on - strand, within MMJJ_RS06080 at 1129.330 kb on - strand, within MMJJ_RS06080 at 1129.331 kb on + strand, within MMJJ_RS06080 at 1129.331 kb on + strand, within MMJJ_RS06080 at 1129.356 kb on + strand, within MMJJ_RS06080 at 1129.382 kb on + strand, within MMJJ_RS06080 at 1129.405 kb on + strand, within MMJJ_RS06080 at 1129.546 kb on - strand, within MMJJ_RS06080 at 1129.547 kb on + strand, within MMJJ_RS06080 at 1129.606 kb on - strand, within MMJJ_RS06080 at 1129.606 kb on - strand, within MMJJ_RS06080 at 1129.607 kb on + strand, within MMJJ_RS06080 at 1129.607 kb on + strand, within MMJJ_RS06080 at 1129.607 kb on + strand, within MMJJ_RS06080 at 1129.607 kb on + strand, within MMJJ_RS06080 at 1129.607 kb on + strand, within MMJJ_RS06080 at 1129.607 kb on + strand, within MMJJ_RS06080 at 1129.607 kb on + strand, within MMJJ_RS06080 at 1129.610 kb on - strand, within MMJJ_RS06080 at 1129.611 kb on + strand, within MMJJ_RS06080 at 1129.611 kb on + strand, within MMJJ_RS06080 at 1129.611 kb on + strand, within MMJJ_RS06080 at 1129.611 kb on + strand, within MMJJ_RS06080 at 1129.644 kb on + strand, within MMJJ_RS06080 at 1129.692 kb on - strand, within MMJJ_RS06080 at 1129.693 kb on + strand, within MMJJ_RS06080 at 1129.714 kb on - strand, within MMJJ_RS06080 at 1129.722 kb on + strand, within MMJJ_RS06080 at 1129.723 kb on - strand, within MMJJ_RS06080 at 1129.779 kb on + strand, within MMJJ_RS06080 at 1129.782 kb on + strand, within MMJJ_RS06080 at 1129.795 kb on - strand, within MMJJ_RS06080 at 1129.795 kb on - strand, within MMJJ_RS06080 at 1129.795 kb on - strand, within MMJJ_RS06080 at 1129.795 kb on - strand, within MMJJ_RS06080 at 1129.798 kb on + strand, within MMJJ_RS06080 at 1129.798 kb on + strand, within MMJJ_RS06080 at 1129.798 kb on + strand, within MMJJ_RS06080 at 1129.798 kb on + strand, within MMJJ_RS06080 at 1129.799 kb on - strand, within MMJJ_RS06080 at 1129.800 kb on + strand, within MMJJ_RS06080 at 1129.800 kb on + strand, within MMJJ_RS06080 at 1129.811 kb on - strand, within MMJJ_RS06080 at 1129.820 kb on - strand, within MMJJ_RS06080 at 1129.820 kb on - strand, within MMJJ_RS06080 at 1129.821 kb on + strand, within MMJJ_RS06080 at 1129.847 kb on - strand at 1129.847 kb on - strand at 1129.848 kb on + strand at 1129.861 kb on - strand at 1129.862 kb on + strand at 1129.876 kb on + strand at 1129.899 kb on + strand at 1129.899 kb on + strand at 1129.941 kb on + strand at 1129.963 kb on - strand at 1130.071 kb on + strand, within MMJJ_RS06085 at 1130.102 kb on + strand, within MMJJ_RS06085 at 1130.124 kb on - strand, within MMJJ_RS06085 at 1130.125 kb on + strand, within MMJJ_RS06085 at 1130.143 kb on + strand, within MMJJ_RS06085 at 1130.170 kb on - strand, within MMJJ_RS06085 at 1130.214 kb on - strand, within MMJJ_RS06085 at 1130.215 kb on + strand, within MMJJ_RS06085 at 1130.271 kb on - strand, within MMJJ_RS06085 at 1130.315 kb on + strand, within MMJJ_RS06085 at 1130.400 kb on + strand, within MMJJ_RS06085 at 1130.420 kb on + strand, within MMJJ_RS06085 at 1130.515 kb on - strand, within MMJJ_RS06085 at 1130.516 kb on + strand, within MMJJ_RS06085 at 1130.656 kb on - strand at 1130.657 kb on + strand at 1130.657 kb on + strand at 1130.661 kb on - strand at 1130.662 kb on + strand at 1130.694 kb on + strand at 1130.716 kb on - strand at 1130.794 kb on - strand, within MMJJ_RS06090 at 1130.855 kb on - strand, within MMJJ_RS06090 at 1130.880 kb on - strand, within MMJJ_RS06090 at 1130.959 kb on - strand, within MMJJ_RS06090 at 1130.983 kb on - strand, within MMJJ_RS06090 at 1130.984 kb on + strand, within MMJJ_RS06090 at 1130.984 kb on + strand, within MMJJ_RS06090 at 1131.022 kb on + strand, within MMJJ_RS06090 at 1131.029 kb on + strand, within MMJJ_RS06090 at 1131.040 kb on - strand, within MMJJ_RS06090 at 1131.041 kb on + strand, within MMJJ_RS06090 at 1131.041 kb on + strand, within MMJJ_RS06090 at 1131.066 kb on + strand, within MMJJ_RS06090 at 1131.066 kb on + strand, within MMJJ_RS06090 at 1131.070 kb on - strand, within MMJJ_RS06090 at 1131.070 kb on - strand, within MMJJ_RS06090 at 1131.070 kb on - strand, within MMJJ_RS06090 at 1131.071 kb on + strand, within MMJJ_RS06090 at 1131.235 kb on + strand, within MMJJ_RS06090 at 1131.296 kb on - strand, within MMJJ_RS06090 at 1131.296 kb on - strand, within MMJJ_RS06090 at 1131.297 kb on + strand, within MMJJ_RS06090 at 1131.301 kb on + strand at 1131.301 kb on + strand at 1131.303 kb on - strand at 1131.312 kb on - strand at 1131.342 kb on - strand at 1131.343 kb on + strand at 1131.369 kb on - strand at 1131.403 kb on + strand at 1131.407 kb on + strand at 1131.410 kb on - strand at 1131.428 kb on + strand at 1131.443 kb on + strand at 1131.597 kb on + strand, within MMJJ_RS06095
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE remove 1,129,009 - MMJJ_RS06075 0.63 +0.4 1,129,039 + MMJJ_RS06075 0.74 +1.7 1,129,048 + MMJJ_RS06075 0.78 -0.4 1,129,064 - MMJJ_RS06075 0.84 +0.3 1,129,064 - MMJJ_RS06075 0.84 +0.1 1,129,073 + MMJJ_RS06075 0.87 +0.9 1,129,091 - -0.4 1,129,092 + +0.6 1,129,098 - +1.2 1,129,131 - -0.9 1,129,132 + -0.0 1,129,145 - -0.1 1,129,145 - +0.4 1,129,145 - +0.7 1,129,145 - -0.4 1,129,146 + +0.2 1,129,146 + +0.2 1,129,147 - +0.5 1,129,147 - +1.9 1,129,147 - +0.5 1,129,148 + -0.6 1,129,148 + +1.1 1,129,149 - +0.6 1,129,149 - -1.1 1,129,149 - +0.3 1,129,150 + +1.5 1,129,162 + -0.5 1,129,195 + -1.4 1,129,195 + +0.3 1,129,257 - -0.2 1,129,312 - MMJJ_RS06080 0.14 +2.6 1,129,330 - MMJJ_RS06080 0.16 +0.6 1,129,330 - MMJJ_RS06080 0.16 +0.7 1,129,331 + MMJJ_RS06080 0.17 -0.8 1,129,331 + MMJJ_RS06080 0.17 +1.2 1,129,356 + MMJJ_RS06080 0.20 +0.4 1,129,382 + MMJJ_RS06080 0.24 +0.7 1,129,405 + MMJJ_RS06080 0.27 +0.4 1,129,546 - MMJJ_RS06080 0.48 -0.4 1,129,547 + MMJJ_RS06080 0.48 -0.7 1,129,606 - MMJJ_RS06080 0.57 -0.9 1,129,606 - MMJJ_RS06080 0.57 -0.2 1,129,607 + MMJJ_RS06080 0.57 -0.5 1,129,607 + MMJJ_RS06080 0.57 +0.5 1,129,607 + MMJJ_RS06080 0.57 +1.5 1,129,607 + MMJJ_RS06080 0.57 -2.3 1,129,607 + MMJJ_RS06080 0.57 +0.6 1,129,607 + MMJJ_RS06080 0.57 +0.8 1,129,607 + MMJJ_RS06080 0.57 -1.1 1,129,610 - MMJJ_RS06080 0.58 +1.5 1,129,611 + MMJJ_RS06080 0.58 -1.2 1,129,611 + MMJJ_RS06080 0.58 +0.0 1,129,611 + MMJJ_RS06080 0.58 -0.2 1,129,611 + MMJJ_RS06080 0.58 +0.1 1,129,644 + MMJJ_RS06080 0.63 +0.0 1,129,692 - MMJJ_RS06080 0.70 -0.1 1,129,693 + MMJJ_RS06080 0.70 +0.2 1,129,714 - MMJJ_RS06080 0.73 +0.1 1,129,722 + MMJJ_RS06080 0.74 -0.6 1,129,723 - MMJJ_RS06080 0.74 +0.1 1,129,779 + MMJJ_RS06080 0.83 +0.1 1,129,782 + MMJJ_RS06080 0.83 -1.3 1,129,795 - MMJJ_RS06080 0.85 -0.2 1,129,795 - MMJJ_RS06080 0.85 +0.4 1,129,795 - MMJJ_RS06080 0.85 -0.4 1,129,795 - MMJJ_RS06080 0.85 +0.8 1,129,798 + MMJJ_RS06080 0.85 -0.0 1,129,798 + MMJJ_RS06080 0.85 -0.6 1,129,798 + MMJJ_RS06080 0.85 +0.6 1,129,798 + MMJJ_RS06080 0.85 +0.2 1,129,799 - MMJJ_RS06080 0.86 +0.6 1,129,800 + MMJJ_RS06080 0.86 +1.3 1,129,800 + MMJJ_RS06080 0.86 +0.4 1,129,811 - MMJJ_RS06080 0.87 -0.1 1,129,820 - MMJJ_RS06080 0.89 +0.4 1,129,820 - MMJJ_RS06080 0.89 +1.1 1,129,821 + MMJJ_RS06080 0.89 -0.0 1,129,847 - -0.1 1,129,847 - +3.2 1,129,848 + -1.1 1,129,861 - +0.1 1,129,862 + +0.4 1,129,876 + +1.9 1,129,899 + +0.6 1,129,899 + +1.2 1,129,941 + -0.5 1,129,963 - +1.3 1,130,071 + MMJJ_RS06085 0.20 -0.7 1,130,102 + MMJJ_RS06085 0.24 +1.9 1,130,124 - MMJJ_RS06085 0.27 +0.1 1,130,125 + MMJJ_RS06085 0.27 +1.0 1,130,143 + MMJJ_RS06085 0.30 +0.6 1,130,170 - MMJJ_RS06085 0.33 -0.1 1,130,214 - MMJJ_RS06085 0.39 -1.2 1,130,215 + MMJJ_RS06085 0.39 +0.0 1,130,271 - MMJJ_RS06085 0.47 -0.1 1,130,315 + MMJJ_RS06085 0.52 +1.1 1,130,400 + MMJJ_RS06085 0.64 +1.2 1,130,420 + MMJJ_RS06085 0.66 -0.0 1,130,515 - MMJJ_RS06085 0.79 -0.9 1,130,516 + MMJJ_RS06085 0.79 +1.0 1,130,656 - -0.0 1,130,657 + +0.8 1,130,657 + -1.7 1,130,661 - +0.8 1,130,662 + +0.3 1,130,694 + -0.4 1,130,716 - -2.8 1,130,794 - MMJJ_RS06090 0.15 -0.7 1,130,855 - MMJJ_RS06090 0.24 -2.3 1,130,880 - MMJJ_RS06090 0.28 -0.8 1,130,959 - MMJJ_RS06090 0.40 +0.1 1,130,983 - MMJJ_RS06090 0.43 -0.2 1,130,984 + MMJJ_RS06090 0.43 +0.1 1,130,984 + MMJJ_RS06090 0.43 -1.0 1,131,022 + MMJJ_RS06090 0.49 -0.1 1,131,029 + MMJJ_RS06090 0.50 -0.6 1,131,040 - MMJJ_RS06090 0.52 -0.7 1,131,041 + MMJJ_RS06090 0.52 +2.3 1,131,041 + MMJJ_RS06090 0.52 -0.9 1,131,066 + MMJJ_RS06090 0.55 -1.2 1,131,066 + MMJJ_RS06090 0.55 +0.3 1,131,070 - MMJJ_RS06090 0.56 -0.9 1,131,070 - MMJJ_RS06090 0.56 -0.7 1,131,070 - MMJJ_RS06090 0.56 +0.8 1,131,071 + MMJJ_RS06090 0.56 -0.7 1,131,235 + MMJJ_RS06090 0.80 -0.6 1,131,296 - MMJJ_RS06090 0.89 -0.9 1,131,296 - MMJJ_RS06090 0.89 -0.5 1,131,297 + MMJJ_RS06090 0.90 -0.1 1,131,301 + +0.7 1,131,301 + -2.4 1,131,303 - -0.0 1,131,312 - -1.5 1,131,342 - +0.6 1,131,343 + +2.3 1,131,369 - +0.1 1,131,403 + +0.7 1,131,407 + +0.7 1,131,410 - -0.8 1,131,428 + -0.2 1,131,443 + -2.1 1,131,597 + MMJJ_RS06095 0.18 -2.5
Or see this region's nucleotide sequence