Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04450 and MMJJ_RS04455 are separated by 89 nucleotides MMJJ_RS04455 and MMJJ_RS04460 are separated by 72 nucleotides MMJJ_RS04460 and MMJJ_RS04465 are separated by 11 nucleotides
MMJJ_RS04450: MMJJ_RS04450 - diphthamide biosynthesis enzyme Dph2, at 828,864 to 829,793
_RS04450
MMJJ_RS04455: MMJJ_RS04455 - phosphomethylpyrimidine synthase, at 829,883 to 831,163
_RS04455
MMJJ_RS04460: MMJJ_RS04460 - L-threonylcarbamoyladenylate synthase, at 831,236 to 831,856
_RS04460
MMJJ_RS04465: MMJJ_RS04465 - MTAP family purine nucleoside phosphorylase, at 831,868 to 832,629
_RS04465
Position (kb) 829
830
831
832 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 828.922 kb on - strand at 829.004 kb on - strand, within MMJJ_RS04450 at 829.009 kb on - strand, within MMJJ_RS04450 at 829.010 kb on + strand, within MMJJ_RS04450 at 829.010 kb on + strand, within MMJJ_RS04450 at 829.010 kb on + strand, within MMJJ_RS04450 at 829.030 kb on + strand, within MMJJ_RS04450 at 829.044 kb on - strand, within MMJJ_RS04450 at 829.044 kb on - strand, within MMJJ_RS04450 at 829.045 kb on + strand, within MMJJ_RS04450 at 829.068 kb on + strand, within MMJJ_RS04450 at 829.068 kb on + strand, within MMJJ_RS04450 at 829.068 kb on + strand, within MMJJ_RS04450 at 829.070 kb on + strand, within MMJJ_RS04450 at 829.079 kb on - strand, within MMJJ_RS04450 at 829.090 kb on + strand, within MMJJ_RS04450 at 829.180 kb on + strand, within MMJJ_RS04450 at 829.182 kb on - strand, within MMJJ_RS04450 at 829.183 kb on + strand, within MMJJ_RS04450 at 829.259 kb on - strand, within MMJJ_RS04450 at 829.260 kb on + strand, within MMJJ_RS04450 at 829.290 kb on - strand, within MMJJ_RS04450 at 829.296 kb on + strand, within MMJJ_RS04450 at 829.297 kb on - strand, within MMJJ_RS04450 at 829.297 kb on - strand, within MMJJ_RS04450 at 829.298 kb on + strand, within MMJJ_RS04450 at 829.307 kb on - strand, within MMJJ_RS04450 at 829.307 kb on - strand at 829.308 kb on + strand, within MMJJ_RS04450 at 829.308 kb on + strand, within MMJJ_RS04450 at 829.384 kb on + strand, within MMJJ_RS04450 at 829.572 kb on + strand, within MMJJ_RS04450 at 829.765 kb on + strand at 829.772 kb on - strand at 829.773 kb on + strand at 829.794 kb on + strand at 829.794 kb on + strand at 829.796 kb on - strand at 829.797 kb on + strand at 829.813 kb on + strand at 829.816 kb on - strand at 829.828 kb on + strand at 829.828 kb on + strand at 829.828 kb on + strand at 829.828 kb on + strand at 829.828 kb on + strand at 829.832 kb on + strand at 829.832 kb on + strand at 829.834 kb on - strand at 829.834 kb on - strand at 829.835 kb on + strand at 829.838 kb on + strand at 829.849 kb on - strand at 829.869 kb on + strand at 829.871 kb on + strand at 830.174 kb on + strand at 830.249 kb on - strand, within MMJJ_RS04455 at 830.858 kb on - strand, within MMJJ_RS04455 at 830.904 kb on - strand, within MMJJ_RS04455 at 831.135 kb on - strand at 831.136 kb on + strand at 831.164 kb on - strand at 831.165 kb on + strand at 831.165 kb on + strand at 831.166 kb on - strand at 831.166 kb on - strand at 831.166 kb on - strand at 831.166 kb on - strand at 831.188 kb on + strand at 831.190 kb on - strand at 831.193 kb on + strand at 831.193 kb on + strand at 831.246 kb on + strand at 831.254 kb on - strand at 831.477 kb on + strand, within MMJJ_RS04460 at 831.731 kb on + strand, within MMJJ_RS04460 at 831.830 kb on + strand at 831.856 kb on - strand at 831.856 kb on - strand at 831.869 kb on - strand at 831.870 kb on + strand at 831.870 kb on + strand at 831.927 kb on + strand at 831.931 kb on + strand at 831.931 kb on + strand at 831.933 kb on - strand at 831.933 kb on - strand at 831.934 kb on + strand at 832.014 kb on - strand, within MMJJ_RS04465 at 832.081 kb on - strand, within MMJJ_RS04465 at 832.082 kb on + strand, within MMJJ_RS04465 at 832.087 kb on - strand, within MMJJ_RS04465 at 832.088 kb on + strand, within MMJJ_RS04465
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE remove 828,922 - +1.6 829,004 - MMJJ_RS04450 0.15 -0.5 829,009 - MMJJ_RS04450 0.16 +1.0 829,010 + MMJJ_RS04450 0.16 -0.3 829,010 + MMJJ_RS04450 0.16 -1.5 829,010 + MMJJ_RS04450 0.16 +2.1 829,030 + MMJJ_RS04450 0.18 +0.9 829,044 - MMJJ_RS04450 0.19 -0.1 829,044 - MMJJ_RS04450 0.19 +2.7 829,045 + MMJJ_RS04450 0.19 +1.4 829,068 + MMJJ_RS04450 0.22 +1.3 829,068 + MMJJ_RS04450 0.22 -0.1 829,068 + MMJJ_RS04450 0.22 +0.1 829,070 + MMJJ_RS04450 0.22 +0.8 829,079 - MMJJ_RS04450 0.23 +1.3 829,090 + MMJJ_RS04450 0.24 -1.1 829,180 + MMJJ_RS04450 0.34 +0.5 829,182 - MMJJ_RS04450 0.34 +1.0 829,183 + MMJJ_RS04450 0.34 -0.3 829,259 - MMJJ_RS04450 0.42 -0.1 829,260 + MMJJ_RS04450 0.43 +1.0 829,290 - MMJJ_RS04450 0.46 +0.3 829,296 + MMJJ_RS04450 0.46 +1.1 829,297 - MMJJ_RS04450 0.47 -0.7 829,297 - MMJJ_RS04450 0.47 +1.2 829,298 + MMJJ_RS04450 0.47 +0.2 829,307 - MMJJ_RS04450 0.48 +3.1 829,307 - +0.9 829,308 + MMJJ_RS04450 0.48 -1.1 829,308 + MMJJ_RS04450 0.48 +1.1 829,384 + MMJJ_RS04450 0.56 +1.0 829,572 + MMJJ_RS04450 0.76 +0.6 829,765 + -1.3 829,772 - +0.5 829,773 + +0.3 829,794 + -2.7 829,794 + -0.4 829,796 - -0.1 829,797 + +0.7 829,813 + +2.8 829,816 - -0.3 829,828 + -1.0 829,828 + +1.9 829,828 + +0.6 829,828 + -1.2 829,828 + -0.2 829,832 + -0.1 829,832 + -3.7 829,834 - -2.4 829,834 - -2.4 829,835 + +1.9 829,838 + -1.7 829,849 - -1.1 829,869 + -2.1 829,871 + -2.4 830,174 + -1.1 830,249 - MMJJ_RS04455 0.29 -1.1 830,858 - MMJJ_RS04455 0.76 -2.1 830,904 - MMJJ_RS04455 0.80 -1.1 831,135 - -4.8 831,136 + -4.2 831,164 - -1.0 831,165 + +0.1 831,165 + +0.4 831,166 - +0.7 831,166 - +0.3 831,166 - -0.3 831,166 - -0.4 831,188 + -1.7 831,190 - -0.1 831,193 + +0.1 831,193 + -1.7 831,246 + -0.1 831,254 - -0.4 831,477 + MMJJ_RS04460 0.39 -2.1 831,731 + MMJJ_RS04460 0.80 -3.1 831,830 + -2.1 831,856 - +0.9 831,856 - -2.1 831,869 - -0.4 831,870 + -1.4 831,870 + +0.2 831,927 + +0.5 831,931 + -1.5 831,931 + -1.0 831,933 - -0.9 831,933 - -1.8 831,934 + +1.6 832,014 - MMJJ_RS04465 0.19 -1.7 832,081 - MMJJ_RS04465 0.28 +0.3 832,082 + MMJJ_RS04465 0.28 +0.3 832,087 - MMJJ_RS04465 0.29 -2.9 832,088 + MMJJ_RS04465 0.29 -1.2
Or see this region's nucleotide sequence