Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS25895 and EX31_RS01145 are separated by 165 nucleotides EX31_RS01145 and EX31_RS01150 are separated by 46 nucleotides
EX31_RS25895: EX31_RS25895 - DinI-like family protein, at 249,420 to 249,527
_RS25895
EX31_RS01145: EX31_RS01145 - DUF3750 domain-containing protein, at 249,693 to 250,466
_RS01145
EX31_RS01150: EX31_RS01150 - oxaloacetate-decarboxylating malate dehydrogenase, at 250,513 to 252,198
_RS01150
Position (kb) 249
250 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 248.443 kb on + strand at 248.443 kb on + strand at 248.444 kb on - strand at 248.444 kb on - strand at 248.460 kb on + strand at 248.461 kb on - strand at 248.461 kb on - strand at 248.490 kb on + strand at 248.490 kb on + strand at 248.491 kb on - strand at 248.494 kb on + strand at 248.495 kb on - strand at 248.495 kb on - strand at 248.520 kb on + strand at 248.573 kb on + strand at 248.573 kb on + strand at 248.578 kb on - strand at 248.611 kb on + strand at 248.612 kb on - strand at 248.654 kb on + strand at 248.747 kb on - strand at 248.749 kb on + strand at 248.750 kb on - strand at 248.793 kb on - strand at 248.793 kb on - strand at 248.793 kb on - strand at 248.851 kb on + strand at 248.927 kb on + strand at 248.929 kb on + strand at 248.929 kb on + strand at 248.930 kb on - strand at 248.960 kb on - strand at 249.062 kb on + strand at 249.062 kb on + strand at 249.063 kb on - strand at 249.063 kb on - strand at 249.067 kb on + strand at 249.068 kb on - strand at 249.088 kb on + strand at 249.089 kb on - strand at 249.092 kb on - strand at 249.092 kb on - strand at 249.181 kb on - strand at 249.237 kb on + strand at 249.238 kb on - strand at 249.248 kb on + strand at 249.248 kb on + strand at 249.248 kb on + strand at 249.248 kb on + strand at 249.249 kb on - strand at 249.249 kb on - strand at 249.249 kb on - strand at 249.283 kb on + strand at 249.284 kb on - strand at 249.284 kb on - strand at 249.284 kb on - strand at 249.284 kb on - strand at 249.292 kb on - strand at 249.326 kb on - strand at 249.373 kb on - strand at 249.373 kb on - strand at 249.373 kb on - strand at 249.404 kb on - strand at 249.404 kb on - strand at 249.442 kb on - strand, within EX31_RS25895 at 249.473 kb on + strand, within EX31_RS25895 at 249.506 kb on + strand, within EX31_RS25895 at 249.506 kb on + strand, within EX31_RS25895 at 249.506 kb on + strand, within EX31_RS25895 at 249.507 kb on - strand, within EX31_RS25895 at 249.563 kb on + strand at 249.564 kb on - strand at 249.564 kb on - strand at 249.564 kb on - strand at 249.611 kb on + strand at 249.611 kb on + strand at 249.612 kb on - strand at 249.612 kb on - strand at 249.651 kb on + strand at 249.688 kb on - strand at 249.703 kb on + strand at 249.703 kb on + strand at 249.712 kb on + strand at 249.712 kb on + strand at 249.736 kb on + strand at 249.736 kb on + strand at 249.870 kb on - strand, within EX31_RS01145 at 249.882 kb on + strand, within EX31_RS01145 at 249.882 kb on + strand, within EX31_RS01145 at 249.939 kb on + strand, within EX31_RS01145 at 250.003 kb on + strand, within EX31_RS01145 at 250.003 kb on + strand, within EX31_RS01145 at 250.003 kb on + strand, within EX31_RS01145 at 250.003 kb on + strand, within EX31_RS01145 at 250.004 kb on - strand, within EX31_RS01145 at 250.004 kb on - strand, within EX31_RS01145 at 250.006 kb on + strand, within EX31_RS01145 at 250.006 kb on + strand, within EX31_RS01145 at 250.006 kb on + strand, within EX31_RS01145 at 250.017 kb on + strand, within EX31_RS01145 at 250.058 kb on + strand, within EX31_RS01145 at 250.058 kb on + strand, within EX31_RS01145 at 250.060 kb on + strand, within EX31_RS01145 at 250.061 kb on - strand, within EX31_RS01145 at 250.094 kb on + strand, within EX31_RS01145 at 250.095 kb on - strand, within EX31_RS01145 at 250.095 kb on - strand, within EX31_RS01145 at 250.143 kb on - strand, within EX31_RS01145 at 250.143 kb on - strand, within EX31_RS01145 at 250.146 kb on + strand, within EX31_RS01145 at 250.146 kb on + strand, within EX31_RS01145 at 250.147 kb on - strand, within EX31_RS01145 at 250.147 kb on - strand, within EX31_RS01145 at 250.147 kb on - strand, within EX31_RS01145 at 250.147 kb on - strand, within EX31_RS01145 at 250.175 kb on + strand, within EX31_RS01145 at 250.175 kb on + strand, within EX31_RS01145 at 250.176 kb on - strand, within EX31_RS01145 at 250.176 kb on - strand, within EX31_RS01145 at 250.176 kb on - strand, within EX31_RS01145 at 250.442 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 248,443 + -0.7 248,443 + -1.0 248,444 - -1.1 248,444 - +1.9 248,460 + +1.9 248,461 - -0.3 248,461 - -1.7 248,490 + -0.2 248,490 + +0.4 248,491 - -0.4 248,494 + +2.3 248,495 - -0.4 248,495 - +0.2 248,520 + +0.8 248,573 + -0.8 248,573 + -0.4 248,578 - +0.2 248,611 + -0.2 248,612 - +0.6 248,654 + -2.3 248,747 - -1.0 248,749 + -1.2 248,750 - +0.8 248,793 - +0.9 248,793 - -1.4 248,793 - +0.4 248,851 + +0.8 248,927 + +0.1 248,929 + +1.9 248,929 + -0.6 248,930 - -0.2 248,960 - -0.2 249,062 + -0.9 249,062 + +0.9 249,063 - +0.7 249,063 - -2.5 249,067 + -0.2 249,068 - -1.1 249,088 + -1.4 249,089 - -0.4 249,092 - +0.1 249,092 - +0.1 249,181 - -2.0 249,237 + +0.2 249,238 - +1.3 249,248 + -0.4 249,248 + +0.3 249,248 + -0.3 249,248 + -0.2 249,249 - +0.7 249,249 - +1.6 249,249 - +1.0 249,283 + +1.2 249,284 - +0.5 249,284 - +0.9 249,284 - -0.6 249,284 - -0.3 249,292 - +0.3 249,326 - -0.8 249,373 - +1.4 249,373 - +0.3 249,373 - -0.5 249,404 - -0.1 249,404 - -1.0 249,442 - EX31_RS25895 0.20 +0.3 249,473 + EX31_RS25895 0.49 -0.1 249,506 + EX31_RS25895 0.80 +0.0 249,506 + EX31_RS25895 0.80 -2.6 249,506 + EX31_RS25895 0.80 +0.4 249,507 - EX31_RS25895 0.81 -0.8 249,563 + -0.4 249,564 - -1.1 249,564 - -0.2 249,564 - -0.4 249,611 + +0.4 249,611 + +0.4 249,612 - -0.2 249,612 - +0.4 249,651 + -0.0 249,688 - -0.4 249,703 + -0.5 249,703 + -0.3 249,712 + +0.1 249,712 + +0.1 249,736 + -1.6 249,736 + -0.4 249,870 - EX31_RS01145 0.23 -0.3 249,882 + EX31_RS01145 0.24 +0.3 249,882 + EX31_RS01145 0.24 -0.2 249,939 + EX31_RS01145 0.32 -0.7 250,003 + EX31_RS01145 0.40 +0.0 250,003 + EX31_RS01145 0.40 +0.2 250,003 + EX31_RS01145 0.40 -0.6 250,003 + EX31_RS01145 0.40 +0.6 250,004 - EX31_RS01145 0.40 +0.2 250,004 - EX31_RS01145 0.40 -1.5 250,006 + EX31_RS01145 0.40 +1.2 250,006 + EX31_RS01145 0.40 -1.8 250,006 + EX31_RS01145 0.40 -0.1 250,017 + EX31_RS01145 0.42 +1.2 250,058 + EX31_RS01145 0.47 -0.0 250,058 + EX31_RS01145 0.47 +3.2 250,060 + EX31_RS01145 0.47 -0.7 250,061 - EX31_RS01145 0.48 +0.7 250,094 + EX31_RS01145 0.52 +0.2 250,095 - EX31_RS01145 0.52 -0.4 250,095 - EX31_RS01145 0.52 +1.1 250,143 - EX31_RS01145 0.58 +1.3 250,143 - EX31_RS01145 0.58 -0.6 250,146 + EX31_RS01145 0.59 -0.1 250,146 + EX31_RS01145 0.59 -0.3 250,147 - EX31_RS01145 0.59 +0.9 250,147 - EX31_RS01145 0.59 +2.5 250,147 - EX31_RS01145 0.59 +0.6 250,147 - EX31_RS01145 0.59 -0.7 250,175 + EX31_RS01145 0.62 -0.2 250,175 + EX31_RS01145 0.62 +2.6 250,176 - EX31_RS01145 0.62 +0.2 250,176 - EX31_RS01145 0.62 -1.3 250,176 - EX31_RS01145 0.62 -0.6 250,442 + +0.1
Or see this region's nucleotide sequence