Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS25715 and EX31_RS25720 are separated by 873 nucleotides EX31_RS25720 and EX31_RS01545 are separated by 154 nucleotides EX31_RS01545 and EX31_RS01550 overlap by 7 nucleotides
EX31_RS25715: EX31_RS25715 - hypothetical protein, at 344,689 to 344,904
_RS25715
EX31_RS25720: EX31_RS25720 - hypothetical protein, at 345,778 to 346,185
_RS25720
EX31_RS01545: EX31_RS01545 - hypothetical protein, at 346,340 to 346,585
_RS01545
EX31_RS01550: EX31_RS01550 - hypothetical protein, at 346,579 to 347,550
_RS01550
Position (kb) 345
346
347 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 344.799 kb on + strand, within EX31_RS25715 at 344.802 kb on + strand, within EX31_RS25715 at 344.834 kb on - strand, within EX31_RS25715 at 344.890 kb on - strand at 344.892 kb on - strand at 344.892 kb on - strand at 344.892 kb on - strand at 344.892 kb on - strand at 344.983 kb on + strand at 345.033 kb on - strand at 345.071 kb on - strand at 345.088 kb on + strand at 345.089 kb on - strand at 345.089 kb on - strand at 345.109 kb on + strand at 345.188 kb on - strand at 345.204 kb on + strand at 345.205 kb on - strand at 345.250 kb on - strand at 345.253 kb on + strand at 345.253 kb on + strand at 345.254 kb on - strand at 345.254 kb on - strand at 345.297 kb on + strand at 345.297 kb on + strand at 345.297 kb on + strand at 345.297 kb on + strand at 345.297 kb on + strand at 345.298 kb on - strand at 345.298 kb on - strand at 345.298 kb on - strand at 345.298 kb on - strand at 345.298 kb on - strand at 345.299 kb on + strand at 345.299 kb on + strand at 345.299 kb on + strand at 345.299 kb on + strand at 345.299 kb on + strand at 345.299 kb on + strand at 345.299 kb on + strand at 345.300 kb on - strand at 345.300 kb on - strand at 345.300 kb on - strand at 345.300 kb on - strand at 345.300 kb on - strand at 345.300 kb on - strand at 345.300 kb on - strand at 345.300 kb on - strand at 345.300 kb on - strand at 345.300 kb on - strand at 345.300 kb on - strand at 345.300 kb on - strand at 345.300 kb on - strand at 345.310 kb on + strand at 345.311 kb on - strand at 345.336 kb on + strand at 345.338 kb on + strand at 345.592 kb on - strand at 345.593 kb on + strand at 345.593 kb on + strand at 345.593 kb on + strand at 345.593 kb on + strand at 345.593 kb on + strand at 345.594 kb on - strand at 345.594 kb on - strand at 345.594 kb on - strand at 345.594 kb on - strand at 345.594 kb on - strand at 345.594 kb on - strand at 345.699 kb on - strand at 345.703 kb on - strand at 345.770 kb on + strand at 345.796 kb on - strand at 345.796 kb on - strand at 345.796 kb on - strand at 345.840 kb on + strand, within EX31_RS25720 at 345.847 kb on + strand, within EX31_RS25720 at 345.848 kb on - strand, within EX31_RS25720 at 345.848 kb on - strand, within EX31_RS25720 at 345.953 kb on - strand, within EX31_RS25720 at 346.025 kb on - strand, within EX31_RS25720 at 346.064 kb on + strand, within EX31_RS25720 at 346.217 kb on + strand at 346.217 kb on + strand at 346.218 kb on - strand at 346.232 kb on + strand at 346.247 kb on - strand at 346.317 kb on - strand at 346.317 kb on - strand at 346.403 kb on - strand, within EX31_RS01545 at 346.423 kb on + strand, within EX31_RS01545 at 346.423 kb on + strand, within EX31_RS01545 at 346.473 kb on + strand, within EX31_RS01545 at 346.601 kb on + strand at 346.602 kb on - strand at 346.602 kb on - strand at 346.605 kb on - strand at 346.605 kb on - strand at 346.605 kb on - strand at 346.616 kb on + strand at 346.616 kb on + strand at 346.617 kb on - strand at 346.617 kb on - strand at 346.631 kb on + strand at 346.632 kb on - strand at 346.632 kb on - strand at 346.632 kb on - strand at 346.694 kb on + strand, within EX31_RS01550 at 346.695 kb on - strand, within EX31_RS01550 at 346.699 kb on - strand, within EX31_RS01550 at 346.700 kb on - strand, within EX31_RS01550 at 346.767 kb on + strand, within EX31_RS01550 at 346.768 kb on - strand, within EX31_RS01550 at 346.772 kb on - strand, within EX31_RS01550 at 346.772 kb on - strand, within EX31_RS01550 at 346.772 kb on - strand, within EX31_RS01550 at 346.772 kb on - strand, within EX31_RS01550 at 346.772 kb on - strand, within EX31_RS01550 at 346.804 kb on + strand, within EX31_RS01550 at 346.855 kb on - strand, within EX31_RS01550 at 346.855 kb on - strand, within EX31_RS01550 at 346.855 kb on - strand, within EX31_RS01550 at 346.870 kb on + strand, within EX31_RS01550 at 346.870 kb on + strand, within EX31_RS01550 at 346.897 kb on - strand, within EX31_RS01550 at 346.897 kb on - strand, within EX31_RS01550 at 346.900 kb on - strand, within EX31_RS01550 at 346.909 kb on + strand, within EX31_RS01550 at 346.924 kb on - strand, within EX31_RS01550 at 346.924 kb on - strand, within EX31_RS01550 at 346.924 kb on - strand, within EX31_RS01550 at 346.958 kb on - strand, within EX31_RS01550 at 346.995 kb on + strand, within EX31_RS01550 at 347.010 kb on + strand, within EX31_RS01550 at 347.080 kb on - strand, within EX31_RS01550 at 347.080 kb on - strand, within EX31_RS01550 at 347.131 kb on - strand, within EX31_RS01550 at 347.137 kb on + strand, within EX31_RS01550 at 347.148 kb on + strand, within EX31_RS01550 at 347.148 kb on + strand, within EX31_RS01550 at 347.150 kb on + strand, within EX31_RS01550 at 347.184 kb on + strand, within EX31_RS01550 at 347.185 kb on - strand, within EX31_RS01550
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 344,799 + EX31_RS25715 0.51 +0.3 344,802 + EX31_RS25715 0.52 +1.2 344,834 - EX31_RS25715 0.67 +1.4 344,890 - +0.9 344,892 - -1.4 344,892 - +0.2 344,892 - +0.6 344,892 - -0.1 344,983 + +0.9 345,033 - +0.9 345,071 - -0.7 345,088 + -1.4 345,089 - -0.8 345,089 - +0.0 345,109 + +0.8 345,188 - -1.0 345,204 + +0.9 345,205 - +0.4 345,250 - +1.0 345,253 + +2.5 345,253 + +0.3 345,254 - -0.7 345,254 - -2.0 345,297 + +1.3 345,297 + +0.7 345,297 + +0.5 345,297 + +1.2 345,297 + -0.8 345,298 - +0.5 345,298 - +1.6 345,298 - -1.6 345,298 - +1.3 345,298 - -1.4 345,299 + +0.7 345,299 + -0.0 345,299 + +0.1 345,299 + -1.1 345,299 + +0.6 345,299 + -0.4 345,299 + +1.0 345,300 - -0.4 345,300 - -1.0 345,300 - -0.8 345,300 - +1.0 345,300 - +1.1 345,300 - +0.3 345,300 - -0.1 345,300 - -0.1 345,300 - +0.5 345,300 - +0.7 345,300 - +0.5 345,300 - -1.7 345,300 - -0.6 345,310 + -1.6 345,311 - +0.7 345,336 + +0.8 345,338 + -0.7 345,592 - +1.1 345,593 + -0.1 345,593 + +0.9 345,593 + -0.7 345,593 + +1.3 345,593 + -0.1 345,594 - +2.2 345,594 - -0.9 345,594 - -1.0 345,594 - -0.1 345,594 - +0.1 345,594 - +1.7 345,699 - +1.9 345,703 - -0.1 345,770 + +0.5 345,796 - -0.4 345,796 - -0.7 345,796 - -1.3 345,840 + EX31_RS25720 0.15 -1.4 345,847 + EX31_RS25720 0.17 -0.9 345,848 - EX31_RS25720 0.17 -0.2 345,848 - EX31_RS25720 0.17 -0.8 345,953 - EX31_RS25720 0.43 +0.8 346,025 - EX31_RS25720 0.61 -0.9 346,064 + EX31_RS25720 0.70 -0.2 346,217 + -0.0 346,217 + +0.8 346,218 - -0.4 346,232 + +0.2 346,247 - -1.1 346,317 - +0.1 346,317 - -0.1 346,403 - EX31_RS01545 0.26 +1.2 346,423 + EX31_RS01545 0.34 -1.8 346,423 + EX31_RS01545 0.34 +1.3 346,473 + EX31_RS01545 0.54 +0.1 346,601 + +0.1 346,602 - +1.6 346,602 - -1.8 346,605 - +0.6 346,605 - -1.3 346,605 - +0.2 346,616 + +2.4 346,616 + +1.4 346,617 - +1.2 346,617 - -1.2 346,631 + +1.9 346,632 - +1.5 346,632 - -0.8 346,632 - +3.5 346,694 + EX31_RS01550 0.12 +0.2 346,695 - EX31_RS01550 0.12 -0.1 346,699 - EX31_RS01550 0.12 +1.5 346,700 - EX31_RS01550 0.12 +0.3 346,767 + EX31_RS01550 0.19 +0.3 346,768 - EX31_RS01550 0.19 +0.3 346,772 - EX31_RS01550 0.20 +0.5 346,772 - EX31_RS01550 0.20 +0.6 346,772 - EX31_RS01550 0.20 +1.7 346,772 - EX31_RS01550 0.20 +1.6 346,772 - EX31_RS01550 0.20 -1.2 346,804 + EX31_RS01550 0.23 +0.1 346,855 - EX31_RS01550 0.28 +0.4 346,855 - EX31_RS01550 0.28 -0.3 346,855 - EX31_RS01550 0.28 +0.3 346,870 + EX31_RS01550 0.30 -0.1 346,870 + EX31_RS01550 0.30 +1.2 346,897 - EX31_RS01550 0.33 +2.0 346,897 - EX31_RS01550 0.33 +0.4 346,900 - EX31_RS01550 0.33 -0.1 346,909 + EX31_RS01550 0.34 +0.6 346,924 - EX31_RS01550 0.35 +2.7 346,924 - EX31_RS01550 0.35 -0.2 346,924 - EX31_RS01550 0.35 +1.2 346,958 - EX31_RS01550 0.39 +1.4 346,995 + EX31_RS01550 0.43 +1.5 347,010 + EX31_RS01550 0.44 +0.5 347,080 - EX31_RS01550 0.52 +0.4 347,080 - EX31_RS01550 0.52 -1.4 347,131 - EX31_RS01550 0.57 +0.5 347,137 + EX31_RS01550 0.57 -0.1 347,148 + EX31_RS01550 0.59 -0.5 347,148 + EX31_RS01550 0.59 -1.6 347,150 + EX31_RS01550 0.59 +0.9 347,184 + EX31_RS01550 0.62 -1.9 347,185 - EX31_RS01550 0.62 -1.1
Or see this region's nucleotide sequence