Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03945 and EX31_RS25125 are separated by 89 nucleotides EX31_RS25125 and EX31_RS03950 are separated by 216 nucleotides
EX31_RS03945: EX31_RS03945 - bifunctional aspartate kinase/homoserine dehydrogenase I, at 305,192 to 307,651
_RS03945
EX31_RS25125: EX31_RS25125 - thr operon leader peptide, at 307,741 to 307,809
_RS25125
EX31_RS03950: EX31_RS03950 - tRNA/rRNA methyltransferase, at 308,026 to 308,712
_RS03950
Position (kb) 307
308 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 306.742 kb on + strand, within EX31_RS03945 at 306.746 kb on - strand, within EX31_RS03945 at 306.876 kb on + strand, within EX31_RS03945 at 306.876 kb on + strand, within EX31_RS03945 at 306.876 kb on + strand, within EX31_RS03945 at 306.877 kb on - strand, within EX31_RS03945 at 306.877 kb on - strand, within EX31_RS03945 at 306.900 kb on + strand, within EX31_RS03945 at 306.901 kb on - strand, within EX31_RS03945 at 306.901 kb on - strand, within EX31_RS03945 at 306.952 kb on + strand, within EX31_RS03945 at 306.952 kb on + strand, within EX31_RS03945 at 306.952 kb on + strand, within EX31_RS03945 at 306.952 kb on + strand, within EX31_RS03945 at 306.952 kb on + strand, within EX31_RS03945 at 306.952 kb on + strand, within EX31_RS03945 at 306.952 kb on + strand, within EX31_RS03945 at 306.952 kb on + strand, within EX31_RS03945 at 306.952 kb on + strand, within EX31_RS03945 at 306.952 kb on + strand, within EX31_RS03945 at 306.952 kb on + strand, within EX31_RS03945 at 306.952 kb on + strand, within EX31_RS03945 at 306.953 kb on - strand, within EX31_RS03945 at 306.953 kb on - strand, within EX31_RS03945 at 306.953 kb on - strand, within EX31_RS03945 at 306.953 kb on - strand, within EX31_RS03945 at 306.953 kb on - strand, within EX31_RS03945 at 306.953 kb on - strand, within EX31_RS03945 at 306.953 kb on - strand, within EX31_RS03945 at 306.953 kb on - strand, within EX31_RS03945 at 306.953 kb on - strand, within EX31_RS03945 at 306.953 kb on - strand, within EX31_RS03945 at 306.953 kb on - strand, within EX31_RS03945 at 306.953 kb on - strand, within EX31_RS03945 at 306.953 kb on - strand, within EX31_RS03945 at 306.953 kb on - strand, within EX31_RS03945 at 306.954 kb on + strand, within EX31_RS03945 at 306.954 kb on + strand, within EX31_RS03945 at 306.954 kb on + strand, within EX31_RS03945 at 306.954 kb on + strand, within EX31_RS03945 at 306.955 kb on - strand, within EX31_RS03945 at 306.955 kb on - strand, within EX31_RS03945 at 306.955 kb on - strand, within EX31_RS03945 at 306.955 kb on - strand, within EX31_RS03945 at 306.955 kb on - strand, within EX31_RS03945 at 306.955 kb on - strand, within EX31_RS03945 at 306.955 kb on - strand, within EX31_RS03945 at 306.955 kb on - strand, within EX31_RS03945 at 306.998 kb on + strand, within EX31_RS03945 at 306.998 kb on + strand, within EX31_RS03945 at 306.998 kb on + strand, within EX31_RS03945 at 306.998 kb on + strand, within EX31_RS03945 at 306.999 kb on - strand, within EX31_RS03945 at 306.999 kb on - strand, within EX31_RS03945 at 306.999 kb on - strand, within EX31_RS03945 at 307.039 kb on + strand, within EX31_RS03945 at 307.039 kb on + strand, within EX31_RS03945 at 307.039 kb on + strand, within EX31_RS03945 at 307.039 kb on + strand, within EX31_RS03945 at 307.040 kb on - strand, within EX31_RS03945 at 307.040 kb on - strand, within EX31_RS03945 at 307.040 kb on - strand, within EX31_RS03945 at 307.173 kb on + strand, within EX31_RS03945 at 307.173 kb on + strand, within EX31_RS03945 at 307.173 kb on + strand, within EX31_RS03945 at 307.174 kb on - strand, within EX31_RS03945 at 307.174 kb on - strand, within EX31_RS03945 at 307.208 kb on - strand, within EX31_RS03945 at 307.252 kb on + strand, within EX31_RS03945 at 307.252 kb on + strand, within EX31_RS03945 at 307.252 kb on + strand, within EX31_RS03945 at 307.253 kb on - strand, within EX31_RS03945 at 307.253 kb on - strand, within EX31_RS03945 at 307.253 kb on - strand, within EX31_RS03945 at 307.298 kb on + strand, within EX31_RS03945 at 307.298 kb on + strand, within EX31_RS03945 at 307.298 kb on + strand, within EX31_RS03945 at 307.298 kb on + strand, within EX31_RS03945 at 307.298 kb on + strand, within EX31_RS03945 at 307.298 kb on + strand, within EX31_RS03945 at 307.298 kb on + strand, within EX31_RS03945 at 307.298 kb on + strand, within EX31_RS03945 at 307.298 kb on + strand, within EX31_RS03945 at 307.298 kb on + strand, within EX31_RS03945 at 307.298 kb on + strand, within EX31_RS03945 at 307.299 kb on - strand, within EX31_RS03945 at 307.299 kb on - strand, within EX31_RS03945 at 307.299 kb on - strand, within EX31_RS03945 at 307.299 kb on - strand, within EX31_RS03945 at 307.299 kb on - strand, within EX31_RS03945 at 307.330 kb on + strand, within EX31_RS03945 at 307.348 kb on + strand, within EX31_RS03945 at 307.348 kb on + strand, within EX31_RS03945 at 307.504 kb on + strand at 307.504 kb on + strand at 307.504 kb on + strand at 307.505 kb on - strand at 307.505 kb on - strand at 307.505 kb on - strand at 307.505 kb on - strand at 307.515 kb on + strand at 307.515 kb on + strand at 307.515 kb on + strand at 307.515 kb on + strand at 307.515 kb on + strand at 307.516 kb on - strand at 307.516 kb on - strand at 307.516 kb on - strand at 307.516 kb on - strand at 307.516 kb on - strand at 307.542 kb on - strand at 307.544 kb on + strand at 307.544 kb on + strand at 307.545 kb on - strand at 307.582 kb on + strand at 307.582 kb on + strand at 307.582 kb on + strand at 307.582 kb on + strand at 307.680 kb on - strand at 307.680 kb on - strand at 307.686 kb on - strand at 307.708 kb on - strand at 307.735 kb on + strand at 307.755 kb on + strand, within EX31_RS25125 at 307.765 kb on - strand, within EX31_RS25125 at 307.776 kb on + strand, within EX31_RS25125 at 307.777 kb on - strand, within EX31_RS25125 at 307.777 kb on - strand, within EX31_RS25125 at 307.777 kb on - strand, within EX31_RS25125 at 307.779 kb on + strand, within EX31_RS25125 at 307.779 kb on + strand, within EX31_RS25125 at 307.779 kb on + strand, within EX31_RS25125 at 307.779 kb on + strand, within EX31_RS25125 at 307.779 kb on + strand, within EX31_RS25125 at 307.779 kb on + strand, within EX31_RS25125 at 307.780 kb on - strand, within EX31_RS25125 at 307.780 kb on - strand, within EX31_RS25125 at 307.814 kb on - strand at 307.830 kb on + strand at 307.830 kb on + strand at 307.831 kb on - strand at 307.831 kb on - strand at 307.838 kb on + strand at 307.838 kb on + strand at 307.839 kb on - strand at 307.839 kb on - strand at 307.839 kb on - strand at 307.853 kb on + strand at 307.854 kb on - strand at 307.854 kb on - strand at 307.856 kb on + strand at 307.856 kb on + strand at 307.856 kb on + strand at 307.856 kb on + strand at 307.856 kb on + strand at 307.856 kb on + strand at 307.856 kb on + strand at 307.856 kb on + strand at 307.856 kb on + strand at 307.857 kb on - strand at 307.857 kb on - strand at 307.857 kb on - strand at 307.857 kb on - strand at 307.857 kb on - strand at 307.857 kb on - strand at 307.857 kb on - strand at 307.857 kb on - strand at 307.867 kb on + strand at 307.867 kb on + strand at 307.868 kb on - strand at 307.868 kb on - strand at 307.878 kb on - strand at 307.878 kb on - strand at 307.878 kb on - strand at 307.878 kb on - strand at 307.907 kb on + strand at 307.908 kb on - strand at 307.931 kb on + strand at 307.935 kb on + strand at 307.935 kb on + strand at 307.935 kb on + strand at 307.935 kb on + strand at 307.935 kb on + strand at 307.935 kb on + strand at 307.935 kb on + strand at 307.935 kb on + strand at 307.935 kb on + strand at 307.935 kb on + strand at 307.935 kb on + strand at 307.936 kb on - strand at 307.936 kb on - strand at 307.936 kb on - strand at 307.936 kb on - strand at 307.936 kb on - strand at 307.936 kb on - strand at 307.936 kb on - strand at 307.936 kb on - strand at 307.961 kb on - strand at 307.970 kb on - strand at 307.979 kb on - strand at 307.979 kb on - strand at 308.003 kb on + strand at 308.056 kb on + strand at 308.056 kb on + strand at 308.056 kb on + strand at 308.057 kb on - strand at 308.057 kb on - strand at 308.057 kb on - strand at 308.064 kb on + strand at 308.064 kb on + strand at 308.064 kb on + strand at 308.064 kb on + strand at 308.064 kb on + strand at 308.064 kb on + strand at 308.064 kb on + strand at 308.064 kb on + strand at 308.064 kb on + strand at 308.064 kb on + strand at 308.065 kb on - strand at 308.065 kb on - strand at 308.065 kb on - strand at 308.065 kb on - strand at 308.065 kb on - strand at 308.065 kb on - strand at 308.065 kb on - strand at 308.065 kb on - strand at 308.249 kb on - strand, within EX31_RS03950 at 308.255 kb on + strand, within EX31_RS03950 at 308.263 kb on + strand, within EX31_RS03950 at 308.264 kb on - strand, within EX31_RS03950 at 308.264 kb on - strand, within EX31_RS03950 at 308.264 kb on - strand, within EX31_RS03950 at 308.264 kb on - strand, within EX31_RS03950 at 308.450 kb on + strand, within EX31_RS03950 at 308.450 kb on + strand, within EX31_RS03950 at 308.450 kb on + strand, within EX31_RS03950 at 308.451 kb on - strand, within EX31_RS03950 at 308.451 kb on - strand, within EX31_RS03950 at 308.453 kb on + strand, within EX31_RS03950 at 308.453 kb on + strand, within EX31_RS03950 at 308.453 kb on + strand, within EX31_RS03950 at 308.454 kb on - strand, within EX31_RS03950 at 308.493 kb on + strand, within EX31_RS03950 at 308.494 kb on - strand, within EX31_RS03950 at 308.494 kb on - strand, within EX31_RS03950 at 308.616 kb on + strand, within EX31_RS03950 at 308.616 kb on + strand, within EX31_RS03950 at 308.617 kb on - strand, within EX31_RS03950 at 308.617 kb on - strand, within EX31_RS03950 at 308.692 kb on + strand at 308.697 kb on + strand at 308.698 kb on - strand at 308.698 kb on - strand at 308.698 kb on - strand at 308.700 kb on + strand at 308.701 kb on - strand at 308.701 kb on - strand at 308.701 kb on - strand at 308.712 kb on + strand at 308.712 kb on + strand at 308.712 kb on + strand at 308.712 kb on + strand at 308.712 kb on + strand at 308.712 kb on + strand at 308.713 kb on - strand at 308.753 kb on - strand at 308.787 kb on + strand at 308.787 kb on + strand at 308.787 kb on + strand at 308.787 kb on + strand at 308.787 kb on + strand at 308.788 kb on - strand at 308.788 kb on - strand at 308.788 kb on - strand at 308.788 kb on - strand at 308.798 kb on + strand at 308.799 kb on - strand at 308.799 kb on - strand at 308.801 kb on - strand at 308.801 kb on - strand at 308.801 kb on - strand at 308.801 kb on - strand at 308.801 kb on - strand at 308.801 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 306,742 + EX31_RS03945 0.63 -1.7 306,746 - EX31_RS03945 0.63 -2.2 306,876 + EX31_RS03945 0.68 -2.1 306,876 + EX31_RS03945 0.68 +0.1 306,876 + EX31_RS03945 0.68 -2.6 306,877 - EX31_RS03945 0.68 -1.8 306,877 - EX31_RS03945 0.68 -1.6 306,900 + EX31_RS03945 0.69 -3.7 306,901 - EX31_RS03945 0.69 -1.9 306,901 - EX31_RS03945 0.69 -3.6 306,952 + EX31_RS03945 0.72 -2.2 306,952 + EX31_RS03945 0.72 -1.2 306,952 + EX31_RS03945 0.72 -2.5 306,952 + EX31_RS03945 0.72 -2.7 306,952 + EX31_RS03945 0.72 -2.0 306,952 + EX31_RS03945 0.72 -2.4 306,952 + EX31_RS03945 0.72 -1.0 306,952 + EX31_RS03945 0.72 -2.9 306,952 + EX31_RS03945 0.72 +0.5 306,952 + EX31_RS03945 0.72 -0.7 306,952 + EX31_RS03945 0.72 -1.6 306,952 + EX31_RS03945 0.72 -1.0 306,953 - EX31_RS03945 0.72 -2.9 306,953 - EX31_RS03945 0.72 -2.6 306,953 - EX31_RS03945 0.72 -2.1 306,953 - EX31_RS03945 0.72 -1.4 306,953 - EX31_RS03945 0.72 -2.8 306,953 - EX31_RS03945 0.72 -0.2 306,953 - EX31_RS03945 0.72 -2.6 306,953 - EX31_RS03945 0.72 -2.9 306,953 - EX31_RS03945 0.72 +0.9 306,953 - EX31_RS03945 0.72 -2.7 306,953 - EX31_RS03945 0.72 -0.9 306,953 - EX31_RS03945 0.72 -2.1 306,953 - EX31_RS03945 0.72 -2.8 306,953 - EX31_RS03945 0.72 -3.4 306,954 + EX31_RS03945 0.72 -1.9 306,954 + EX31_RS03945 0.72 -0.6 306,954 + EX31_RS03945 0.72 -3.9 306,954 + EX31_RS03945 0.72 -0.6 306,955 - EX31_RS03945 0.72 -1.9 306,955 - EX31_RS03945 0.72 -2.8 306,955 - EX31_RS03945 0.72 -1.2 306,955 - EX31_RS03945 0.72 -1.6 306,955 - EX31_RS03945 0.72 -1.6 306,955 - EX31_RS03945 0.72 -0.9 306,955 - EX31_RS03945 0.72 +0.6 306,955 - EX31_RS03945 0.72 -2.0 306,998 + EX31_RS03945 0.73 -1.7 306,998 + EX31_RS03945 0.73 -2.6 306,998 + EX31_RS03945 0.73 -0.1 306,998 + EX31_RS03945 0.73 -2.4 306,999 - EX31_RS03945 0.73 -1.1 306,999 - EX31_RS03945 0.73 -0.6 306,999 - EX31_RS03945 0.73 -2.4 307,039 + EX31_RS03945 0.75 -2.8 307,039 + EX31_RS03945 0.75 -1.6 307,039 + EX31_RS03945 0.75 -1.6 307,039 + EX31_RS03945 0.75 -2.5 307,040 - EX31_RS03945 0.75 -2.6 307,040 - EX31_RS03945 0.75 -1.4 307,040 - EX31_RS03945 0.75 -2.5 307,173 + EX31_RS03945 0.81 -0.7 307,173 + EX31_RS03945 0.81 -2.5 307,173 + EX31_RS03945 0.81 -0.6 307,174 - EX31_RS03945 0.81 -3.6 307,174 - EX31_RS03945 0.81 -1.6 307,208 - EX31_RS03945 0.82 -0.9 307,252 + EX31_RS03945 0.84 -1.5 307,252 + EX31_RS03945 0.84 -3.7 307,252 + EX31_RS03945 0.84 -2.5 307,253 - EX31_RS03945 0.84 -2.2 307,253 - EX31_RS03945 0.84 -2.2 307,253 - EX31_RS03945 0.84 -3.4 307,298 + EX31_RS03945 0.86 -1.1 307,298 + EX31_RS03945 0.86 -0.4 307,298 + EX31_RS03945 0.86 -1.6 307,298 + EX31_RS03945 0.86 -2.5 307,298 + EX31_RS03945 0.86 -1.0 307,298 + EX31_RS03945 0.86 -2.1 307,298 + EX31_RS03945 0.86 -2.0 307,298 + EX31_RS03945 0.86 -2.6 307,298 + EX31_RS03945 0.86 -1.2 307,298 + EX31_RS03945 0.86 -3.2 307,298 + EX31_RS03945 0.86 -2.5 307,299 - EX31_RS03945 0.86 -0.9 307,299 - EX31_RS03945 0.86 -0.5 307,299 - EX31_RS03945 0.86 -0.2 307,299 - EX31_RS03945 0.86 -2.7 307,299 - EX31_RS03945 0.86 -2.6 307,330 + EX31_RS03945 0.87 -2.1 307,348 + EX31_RS03945 0.88 +0.2 307,348 + EX31_RS03945 0.88 -1.1 307,504 + -2.2 307,504 + -2.8 307,504 + -3.7 307,505 - -3.7 307,505 - -1.9 307,505 - -2.6 307,505 - -2.5 307,515 + -1.7 307,515 + -0.9 307,515 + -3.5 307,515 + -1.1 307,515 + -4.4 307,516 - -4.4 307,516 - -0.8 307,516 - -2.9 307,516 - -1.9 307,516 - -2.7 307,542 - -1.4 307,544 + -2.0 307,544 + -1.9 307,545 - -1.6 307,582 + -3.2 307,582 + -1.4 307,582 + -1.2 307,582 + -2.6 307,680 - -0.9 307,680 - +0.6 307,686 - +0.2 307,708 - +0.4 307,735 + -1.4 307,755 + EX31_RS25125 0.20 -0.4 307,765 - EX31_RS25125 0.35 -0.0 307,776 + EX31_RS25125 0.51 -3.0 307,777 - EX31_RS25125 0.52 +1.5 307,777 - EX31_RS25125 0.52 +0.3 307,777 - EX31_RS25125 0.52 -0.3 307,779 + EX31_RS25125 0.55 -1.4 307,779 + EX31_RS25125 0.55 +0.6 307,779 + EX31_RS25125 0.55 -3.3 307,779 + EX31_RS25125 0.55 -3.2 307,779 + EX31_RS25125 0.55 -1.9 307,779 + EX31_RS25125 0.55 -2.2 307,780 - EX31_RS25125 0.57 -0.5 307,780 - EX31_RS25125 0.57 -0.0 307,814 - -0.1 307,830 + -4.2 307,830 + -0.7 307,831 - +1.2 307,831 - -0.6 307,838 + -3.6 307,838 + -0.2 307,839 - +0.2 307,839 - +0.1 307,839 - +1.2 307,853 + -3.2 307,854 - +0.5 307,854 - +0.3 307,856 + -3.3 307,856 + -2.2 307,856 + -0.8 307,856 + -3.7 307,856 + -0.9 307,856 + -0.9 307,856 + +1.2 307,856 + -0.5 307,856 + -3.0 307,857 - -0.1 307,857 - -2.0 307,857 - -0.1 307,857 - +0.6 307,857 - -0.1 307,857 - +0.5 307,857 - +0.2 307,857 - -0.8 307,867 + -1.8 307,867 + -0.6 307,868 - +1.0 307,868 - -0.2 307,878 - -0.0 307,878 - -0.7 307,878 - -0.6 307,878 - +0.3 307,907 + -2.3 307,908 - +0.1 307,931 + +0.6 307,935 + +0.6 307,935 + -0.3 307,935 + -0.8 307,935 + +0.6 307,935 + +0.5 307,935 + -0.5 307,935 + -0.6 307,935 + -0.3 307,935 + -0.8 307,935 + +0.2 307,935 + +0.6 307,936 - -0.0 307,936 - +0.2 307,936 - -0.2 307,936 - +0.5 307,936 - +0.4 307,936 - +0.2 307,936 - -1.2 307,936 - +0.6 307,961 - +0.4 307,970 - +1.1 307,979 - +0.3 307,979 - +0.2 308,003 + +0.3 308,056 + -0.8 308,056 + +0.1 308,056 + +0.4 308,057 - +0.6 308,057 - -1.1 308,057 - -0.6 308,064 + +0.7 308,064 + -0.6 308,064 + +0.9 308,064 + +0.2 308,064 + -1.3 308,064 + +1.5 308,064 + -2.2 308,064 + +0.3 308,064 + +0.4 308,064 + +1.2 308,065 - +1.0 308,065 - -0.6 308,065 - +1.6 308,065 - -0.2 308,065 - +1.2 308,065 - -0.4 308,065 - -0.6 308,065 - +0.8 308,249 - EX31_RS03950 0.32 +0.4 308,255 + EX31_RS03950 0.33 +0.6 308,263 + EX31_RS03950 0.34 +1.2 308,264 - EX31_RS03950 0.35 -0.7 308,264 - EX31_RS03950 0.35 -0.4 308,264 - EX31_RS03950 0.35 +2.6 308,264 - EX31_RS03950 0.35 +1.1 308,450 + EX31_RS03950 0.62 +0.7 308,450 + EX31_RS03950 0.62 +0.1 308,450 + EX31_RS03950 0.62 +1.3 308,451 - EX31_RS03950 0.62 +0.5 308,451 - EX31_RS03950 0.62 +0.2 308,453 + EX31_RS03950 0.62 -0.5 308,453 + EX31_RS03950 0.62 +0.9 308,453 + EX31_RS03950 0.62 -0.5 308,454 - EX31_RS03950 0.62 -0.6 308,493 + EX31_RS03950 0.68 +0.8 308,494 - EX31_RS03950 0.68 +0.7 308,494 - EX31_RS03950 0.68 +0.1 308,616 + EX31_RS03950 0.86 +1.2 308,616 + EX31_RS03950 0.86 -0.2 308,617 - EX31_RS03950 0.86 +1.0 308,617 - EX31_RS03950 0.86 +0.1 308,692 + -0.6 308,697 + -0.4 308,698 - +0.3 308,698 - -0.7 308,698 - -0.2 308,700 + +0.3 308,701 - +0.4 308,701 - +0.6 308,701 - -0.6 308,712 + +0.1 308,712 + +0.3 308,712 + -0.8 308,712 + -0.2 308,712 + -1.2 308,712 + -0.6 308,713 - +0.3 308,753 - -1.0 308,787 + -0.0 308,787 + -0.3 308,787 + -0.1 308,787 + +0.6 308,787 + +1.6 308,788 - -0.5 308,788 - +0.2 308,788 - -0.3 308,788 - +0.0 308,798 + +0.6 308,799 - +0.9 308,799 - +1.2 308,801 - -0.5 308,801 - +0.8 308,801 - -0.2 308,801 - +1.6 308,801 - -1.8 308,801 - +0.6
Or see this region's nucleotide sequence