Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS24715 and EX31_RS24720 are separated by 207 nucleotides EX31_RS24720 and EX31_RS24725 overlap by 4 nucleotides EX31_RS24725 and EX31_RS24730 are separated by 161 nucleotides EX31_RS24730 and EX31_RS24735 are separated by 90 nucleotides
EX31_RS24715: EX31_RS24715 - L-serine ammonia-lyase, at 712,709 to 714,073
_RS24715
EX31_RS24720: EX31_RS24720 - CoA pyrophosphatase, at 714,281 to 714,883
_RS24720
EX31_RS24725: EX31_RS24725 - aminodeoxychorismate synthase component 1, at 714,880 to 716,256
_RS24725
EX31_RS24730: EX31_RS24730 - YoaH family protein, at 716,418 to 716,600
_RS24730
EX31_RS24735: EX31_RS24735 - formate-dependent phosphoribosylglycinamide formyltransferase, at 716,691 to 717,869
_RS24735
Position (kb) 714
715
716
717 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 713.902 kb on + strand, within EX31_RS24715 at 713.903 kb on - strand, within EX31_RS24715 at 713.903 kb on - strand, within EX31_RS24715 at 714.081 kb on - strand at 714.092 kb on - strand at 714.092 kb on - strand at 714.092 kb on - strand at 714.126 kb on + strand at 714.126 kb on + strand at 714.126 kb on + strand at 714.127 kb on - strand at 714.187 kb on + strand at 714.187 kb on + strand at 714.187 kb on + strand at 714.187 kb on + strand at 714.187 kb on + strand at 714.188 kb on - strand at 714.188 kb on - strand at 714.188 kb on - strand at 714.188 kb on - strand at 714.188 kb on - strand at 714.188 kb on - strand at 714.188 kb on - strand at 714.189 kb on + strand at 714.189 kb on + strand at 714.189 kb on + strand at 714.189 kb on + strand at 714.190 kb on - strand at 714.190 kb on - strand at 714.190 kb on - strand at 714.190 kb on - strand at 714.195 kb on - strand at 714.195 kb on - strand at 714.195 kb on - strand at 714.235 kb on + strand at 714.242 kb on - strand at 714.242 kb on - strand at 714.264 kb on + strand at 714.270 kb on - strand at 714.363 kb on + strand, within EX31_RS24720 at 714.363 kb on + strand, within EX31_RS24720 at 714.485 kb on + strand, within EX31_RS24720 at 714.485 kb on + strand, within EX31_RS24720 at 714.486 kb on - strand, within EX31_RS24720 at 714.486 kb on - strand, within EX31_RS24720 at 714.486 kb on - strand, within EX31_RS24720 at 714.563 kb on + strand, within EX31_RS24720 at 714.563 kb on + strand, within EX31_RS24720 at 714.563 kb on + strand, within EX31_RS24720 at 714.564 kb on - strand, within EX31_RS24720 at 714.564 kb on - strand, within EX31_RS24720 at 714.564 kb on - strand, within EX31_RS24720 at 714.564 kb on - strand, within EX31_RS24720 at 714.564 kb on - strand, within EX31_RS24720 at 714.564 kb on - strand, within EX31_RS24720 at 714.564 kb on - strand, within EX31_RS24720 at 714.565 kb on + strand, within EX31_RS24720 at 714.565 kb on + strand, within EX31_RS24720 at 714.565 kb on + strand, within EX31_RS24720 at 714.565 kb on + strand, within EX31_RS24720 at 714.565 kb on + strand, within EX31_RS24720 at 714.565 kb on + strand, within EX31_RS24720 at 714.565 kb on + strand, within EX31_RS24720 at 714.565 kb on + strand, within EX31_RS24720 at 714.566 kb on - strand, within EX31_RS24720 at 714.566 kb on - strand, within EX31_RS24720 at 714.566 kb on - strand, within EX31_RS24720 at 714.566 kb on - strand, within EX31_RS24720 at 714.566 kb on - strand, within EX31_RS24720 at 714.566 kb on - strand, within EX31_RS24720 at 714.566 kb on - strand, within EX31_RS24720 at 714.566 kb on - strand, within EX31_RS24720 at 714.566 kb on - strand, within EX31_RS24720 at 714.566 kb on - strand, within EX31_RS24720 at 714.566 kb on - strand, within EX31_RS24720 at 714.625 kb on + strand, within EX31_RS24720 at 714.626 kb on - strand, within EX31_RS24720 at 714.686 kb on - strand, within EX31_RS24720 at 714.855 kb on - strand at 714.855 kb on - strand at 714.890 kb on - strand at 714.890 kb on - strand at 714.942 kb on - strand at 715.064 kb on + strand, within EX31_RS24725 at 715.065 kb on - strand, within EX31_RS24725 at 715.065 kb on - strand, within EX31_RS24725 at 715.065 kb on - strand, within EX31_RS24725 at 715.334 kb on - strand, within EX31_RS24725 at 715.334 kb on - strand, within EX31_RS24725 at 715.642 kb on - strand, within EX31_RS24725 at 715.642 kb on - strand, within EX31_RS24725 at 715.644 kb on - strand, within EX31_RS24725 at 715.644 kb on - strand, within EX31_RS24725 at 715.644 kb on - strand, within EX31_RS24725 at 715.644 kb on - strand, within EX31_RS24725 at 715.672 kb on + strand, within EX31_RS24725 at 715.690 kb on - strand, within EX31_RS24725 at 715.690 kb on - strand, within EX31_RS24725 at 715.711 kb on + strand, within EX31_RS24725 at 715.712 kb on - strand, within EX31_RS24725 at 715.712 kb on - strand, within EX31_RS24725 at 715.762 kb on + strand, within EX31_RS24725 at 715.763 kb on - strand, within EX31_RS24725 at 715.807 kb on + strand, within EX31_RS24725 at 715.808 kb on - strand, within EX31_RS24725 at 715.808 kb on - strand, within EX31_RS24725 at 715.838 kb on + strand, within EX31_RS24725 at 715.839 kb on - strand, within EX31_RS24725 at 715.839 kb on - strand, within EX31_RS24725 at 715.839 kb on - strand, within EX31_RS24725 at 715.978 kb on + strand, within EX31_RS24725 at 716.066 kb on - strand, within EX31_RS24725 at 716.125 kb on - strand at 716.125 kb on - strand at 716.155 kb on + strand at 716.155 kb on + strand at 716.156 kb on - strand at 716.201 kb on - strand at 716.201 kb on - strand at 716.221 kb on + strand at 716.221 kb on + strand at 716.222 kb on - strand at 716.288 kb on - strand at 716.356 kb on + strand at 716.356 kb on + strand at 716.356 kb on + strand at 716.357 kb on - strand at 716.357 kb on - strand at 716.358 kb on + strand at 716.358 kb on + strand at 716.359 kb on - strand at 716.402 kb on - strand at 716.429 kb on + strand at 716.429 kb on + strand at 716.429 kb on + strand at 716.430 kb on - strand at 716.430 kb on - strand at 716.430 kb on - strand at 716.430 kb on - strand at 716.440 kb on + strand, within EX31_RS24730 at 716.441 kb on - strand, within EX31_RS24730 at 716.441 kb on - strand, within EX31_RS24730 at 716.552 kb on + strand, within EX31_RS24730 at 716.552 kb on + strand, within EX31_RS24730 at 716.552 kb on + strand, within EX31_RS24730 at 716.599 kb on - strand at 716.790 kb on - strand at 716.980 kb on - strand, within EX31_RS24735
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 713,902 + EX31_RS24715 0.87 +0.1 713,903 - EX31_RS24715 0.87 +3.4 713,903 - EX31_RS24715 0.87 -0.5 714,081 - -0.5 714,092 - -1.5 714,092 - +1.0 714,092 - +0.8 714,126 + -0.5 714,126 + -0.3 714,126 + -1.2 714,127 - -0.1 714,187 + +1.8 714,187 + +0.7 714,187 + +0.1 714,187 + -0.1 714,187 + +0.1 714,188 - +0.7 714,188 - +0.2 714,188 - +0.7 714,188 - +0.6 714,188 - +1.0 714,188 - -0.5 714,188 - -1.0 714,189 + -1.1 714,189 + -0.8 714,189 + +0.2 714,189 + -0.7 714,190 - -0.2 714,190 - +0.5 714,190 - +0.7 714,190 - +1.6 714,195 - -0.1 714,195 - +1.6 714,195 - +0.2 714,235 + -0.9 714,242 - +1.2 714,242 - +0.8 714,264 + -0.1 714,270 - +1.8 714,363 + EX31_RS24720 0.14 +0.2 714,363 + EX31_RS24720 0.14 +0.0 714,485 + EX31_RS24720 0.34 -0.4 714,485 + EX31_RS24720 0.34 -0.4 714,486 - EX31_RS24720 0.34 +0.2 714,486 - EX31_RS24720 0.34 +0.7 714,486 - EX31_RS24720 0.34 -0.0 714,563 + EX31_RS24720 0.47 +1.1 714,563 + EX31_RS24720 0.47 +0.2 714,563 + EX31_RS24720 0.47 -1.5 714,564 - EX31_RS24720 0.47 -1.5 714,564 - EX31_RS24720 0.47 -1.0 714,564 - EX31_RS24720 0.47 +0.9 714,564 - EX31_RS24720 0.47 +1.1 714,564 - EX31_RS24720 0.47 -1.8 714,564 - EX31_RS24720 0.47 -0.2 714,564 - EX31_RS24720 0.47 +0.9 714,565 + EX31_RS24720 0.47 +0.9 714,565 + EX31_RS24720 0.47 -0.5 714,565 + EX31_RS24720 0.47 +1.1 714,565 + EX31_RS24720 0.47 +0.3 714,565 + EX31_RS24720 0.47 +0.2 714,565 + EX31_RS24720 0.47 -1.2 714,565 + EX31_RS24720 0.47 -0.6 714,565 + EX31_RS24720 0.47 +0.2 714,566 - EX31_RS24720 0.47 +0.4 714,566 - EX31_RS24720 0.47 +0.7 714,566 - EX31_RS24720 0.47 -1.3 714,566 - EX31_RS24720 0.47 -0.0 714,566 - EX31_RS24720 0.47 -0.6 714,566 - EX31_RS24720 0.47 -0.3 714,566 - EX31_RS24720 0.47 -1.0 714,566 - EX31_RS24720 0.47 +0.4 714,566 - EX31_RS24720 0.47 +1.0 714,566 - EX31_RS24720 0.47 -0.5 714,566 - EX31_RS24720 0.47 -0.2 714,625 + EX31_RS24720 0.57 +0.6 714,626 - EX31_RS24720 0.57 +1.1 714,686 - EX31_RS24720 0.67 +0.5 714,855 - +0.7 714,855 - +0.6 714,890 - +0.3 714,890 - +0.4 714,942 - -0.1 715,064 + EX31_RS24725 0.13 +1.1 715,065 - EX31_RS24725 0.13 -1.5 715,065 - EX31_RS24725 0.13 -0.5 715,065 - EX31_RS24725 0.13 -1.3 715,334 - EX31_RS24725 0.33 +0.5 715,334 - EX31_RS24725 0.33 +1.7 715,642 - EX31_RS24725 0.55 +0.5 715,642 - EX31_RS24725 0.55 -0.0 715,644 - EX31_RS24725 0.55 +0.5 715,644 - EX31_RS24725 0.55 +1.2 715,644 - EX31_RS24725 0.55 -0.8 715,644 - EX31_RS24725 0.55 +1.5 715,672 + EX31_RS24725 0.58 +0.9 715,690 - EX31_RS24725 0.59 +0.8 715,690 - EX31_RS24725 0.59 +1.1 715,711 + EX31_RS24725 0.60 +1.0 715,712 - EX31_RS24725 0.60 -0.4 715,712 - EX31_RS24725 0.60 +1.2 715,762 + EX31_RS24725 0.64 +1.0 715,763 - EX31_RS24725 0.64 +1.0 715,807 + EX31_RS24725 0.67 +0.1 715,808 - EX31_RS24725 0.67 +0.8 715,808 - EX31_RS24725 0.67 +0.7 715,838 + EX31_RS24725 0.70 +0.7 715,839 - EX31_RS24725 0.70 -0.8 715,839 - EX31_RS24725 0.70 +0.5 715,839 - EX31_RS24725 0.70 +0.2 715,978 + EX31_RS24725 0.80 +2.8 716,066 - EX31_RS24725 0.86 +1.8 716,125 - -0.8 716,125 - -0.1 716,155 + +1.6 716,155 + +1.2 716,156 - +0.2 716,201 - +0.1 716,201 - +1.0 716,221 + +1.0 716,221 + -0.2 716,222 - -1.5 716,288 - +0.8 716,356 + +0.1 716,356 + -0.5 716,356 + +0.4 716,357 - +0.4 716,357 - -0.1 716,358 + +1.2 716,358 + +0.9 716,359 - -0.2 716,402 - +0.5 716,429 + +0.5 716,429 + +0.9 716,429 + -1.1 716,430 - -0.3 716,430 - +1.8 716,430 - +0.9 716,430 - +1.1 716,440 + EX31_RS24730 0.12 +0.2 716,441 - EX31_RS24730 0.13 +0.7 716,441 - EX31_RS24730 0.13 +0.2 716,552 + EX31_RS24730 0.73 -0.4 716,552 + EX31_RS24730 0.73 -0.2 716,552 + EX31_RS24730 0.73 +0.2 716,599 - +1.6 716,790 - -0.3 716,980 - EX31_RS24735 0.25 +0.2
Or see this region's nucleotide sequence