Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23805 and EX31_RS23810 are separated by 261 nucleotides EX31_RS23810 and EX31_RS23815 are separated by 369 nucleotides
EX31_RS23805: EX31_RS23805 - D-alanyl-D-alanine endopeptidase, at 528,421 to 529,353
_RS23805
EX31_RS23810: EX31_RS23810 - hypothetical protein, at 529,615 to 530,244
_RS23810
EX31_RS23815: EX31_RS23815 - hypothetical protein, at 530,614 to 531,252
_RS23815
Position (kb) 529
530
531 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 528.699 kb on + strand, within EX31_RS23805 at 528.699 kb on + strand, within EX31_RS23805 at 528.702 kb on + strand, within EX31_RS23805 at 528.702 kb on + strand, within EX31_RS23805 at 528.702 kb on + strand, within EX31_RS23805 at 528.702 kb on + strand, within EX31_RS23805 at 528.702 kb on + strand, within EX31_RS23805 at 528.702 kb on + strand, within EX31_RS23805 at 528.703 kb on - strand, within EX31_RS23805 at 528.703 kb on - strand, within EX31_RS23805 at 528.703 kb on - strand, within EX31_RS23805 at 528.703 kb on - strand, within EX31_RS23805 at 528.703 kb on - strand, within EX31_RS23805 at 528.838 kb on + strand, within EX31_RS23805 at 528.849 kb on + strand, within EX31_RS23805 at 528.849 kb on + strand, within EX31_RS23805 at 528.849 kb on + strand, within EX31_RS23805 at 528.849 kb on + strand, within EX31_RS23805 at 528.850 kb on - strand, within EX31_RS23805 at 528.858 kb on + strand, within EX31_RS23805 at 528.858 kb on + strand, within EX31_RS23805 at 528.953 kb on + strand, within EX31_RS23805 at 528.953 kb on + strand, within EX31_RS23805 at 528.954 kb on - strand, within EX31_RS23805 at 529.001 kb on + strand, within EX31_RS23805 at 529.002 kb on - strand, within EX31_RS23805 at 529.005 kb on + strand, within EX31_RS23805 at 529.006 kb on - strand, within EX31_RS23805 at 529.006 kb on - strand, within EX31_RS23805 at 529.045 kb on + strand, within EX31_RS23805 at 529.046 kb on - strand, within EX31_RS23805 at 529.062 kb on + strand, within EX31_RS23805 at 529.063 kb on - strand, within EX31_RS23805 at 529.065 kb on + strand, within EX31_RS23805 at 529.065 kb on + strand, within EX31_RS23805 at 529.066 kb on - strand, within EX31_RS23805 at 529.155 kb on + strand, within EX31_RS23805 at 529.460 kb on - strand at 529.518 kb on - strand at 529.523 kb on - strand at 529.575 kb on + strand at 529.575 kb on + strand at 529.576 kb on - strand at 529.576 kb on - strand at 529.578 kb on - strand at 529.578 kb on - strand at 529.578 kb on - strand at 529.610 kb on + strand at 529.736 kb on + strand, within EX31_RS23810 at 529.737 kb on - strand, within EX31_RS23810 at 529.769 kb on - strand, within EX31_RS23810 at 529.769 kb on - strand, within EX31_RS23810 at 529.769 kb on - strand, within EX31_RS23810 at 529.769 kb on - strand, within EX31_RS23810 at 529.769 kb on - strand, within EX31_RS23810 at 529.773 kb on - strand, within EX31_RS23810 at 529.794 kb on + strand, within EX31_RS23810 at 530.160 kb on + strand, within EX31_RS23810 at 530.160 kb on + strand, within EX31_RS23810 at 530.161 kb on - strand, within EX31_RS23810 at 530.161 kb on - strand, within EX31_RS23810 at 530.161 kb on - strand, within EX31_RS23810 at 530.190 kb on + strand at 530.208 kb on + strand at 530.209 kb on - strand at 530.223 kb on + strand at 530.224 kb on - strand at 530.224 kb on - strand at 530.227 kb on + strand at 530.227 kb on + strand at 530.227 kb on + strand at 530.227 kb on + strand at 530.227 kb on + strand at 530.227 kb on + strand at 530.227 kb on + strand at 530.227 kb on + strand at 530.227 kb on + strand at 530.227 kb on + strand at 530.227 kb on + strand at 530.228 kb on - strand at 530.228 kb on - strand at 530.228 kb on - strand at 530.228 kb on - strand at 530.228 kb on - strand at 530.228 kb on - strand at 530.228 kb on - strand at 530.228 kb on - strand at 530.228 kb on - strand at 530.228 kb on - strand at 530.228 kb on - strand at 530.228 kb on - strand at 530.235 kb on + strand at 530.235 kb on + strand at 530.236 kb on - strand at 530.236 kb on - strand at 530.236 kb on - strand at 530.236 kb on - strand at 530.246 kb on + strand at 530.247 kb on - strand at 530.247 kb on - strand at 530.247 kb on - strand at 530.247 kb on - strand at 530.258 kb on + strand at 530.272 kb on + strand at 530.273 kb on - strand at 530.295 kb on - strand at 530.309 kb on + strand at 530.316 kb on - strand at 530.452 kb on + strand at 530.485 kb on - strand at 530.485 kb on - strand at 530.485 kb on - strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.486 kb on + strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.487 kb on - strand at 530.488 kb on + strand at 530.488 kb on + strand at 530.488 kb on + strand at 530.488 kb on + strand at 530.488 kb on + strand at 530.488 kb on + strand at 530.488 kb on + strand at 530.488 kb on + strand at 530.488 kb on + strand at 530.488 kb on + strand at 530.488 kb on + strand at 530.488 kb on + strand at 530.488 kb on + strand at 530.489 kb on - strand at 530.489 kb on - strand at 530.489 kb on - strand at 530.489 kb on - strand at 530.489 kb on - strand at 530.489 kb on - strand at 530.489 kb on - strand at 530.489 kb on - strand at 530.489 kb on - strand at 530.489 kb on - strand at 530.489 kb on - strand at 530.492 kb on + strand at 530.492 kb on + strand at 530.492 kb on + strand at 530.492 kb on + strand at 530.492 kb on + strand at 530.493 kb on - strand at 530.493 kb on - strand at 530.493 kb on - strand at 530.493 kb on - strand at 530.493 kb on - strand at 530.493 kb on - strand at 530.493 kb on - strand at 530.496 kb on + strand at 530.496 kb on + strand at 530.496 kb on + strand at 530.497 kb on - strand at 530.508 kb on + strand at 530.541 kb on + strand at 530.580 kb on + strand at 530.613 kb on + strand at 530.613 kb on + strand at 530.613 kb on + strand at 530.613 kb on + strand at 530.613 kb on + strand at 530.613 kb on + strand at 530.613 kb on + strand at 530.613 kb on + strand at 530.613 kb on + strand at 530.613 kb on + strand at 530.613 kb on + strand at 530.613 kb on + strand at 530.613 kb on + strand at 530.614 kb on - strand at 530.614 kb on - strand at 530.614 kb on - strand at 530.614 kb on - strand at 530.614 kb on - strand at 530.640 kb on - strand at 530.662 kb on + strand at 530.662 kb on + strand at 530.695 kb on + strand, within EX31_RS23815 at 530.696 kb on - strand, within EX31_RS23815 at 530.932 kb on - strand, within EX31_RS23815 at 530.999 kb on + strand, within EX31_RS23815 at 531.003 kb on + strand, within EX31_RS23815 at 531.004 kb on - strand, within EX31_RS23815 at 531.021 kb on - strand, within EX31_RS23815 at 531.021 kb on - strand, within EX31_RS23815 at 531.021 kb on - strand, within EX31_RS23815 at 531.024 kb on + strand, within EX31_RS23815 at 531.024 kb on + strand, within EX31_RS23815 at 531.174 kb on + strand, within EX31_RS23815 at 531.241 kb on + strand at 531.241 kb on + strand at 531.241 kb on + strand at 531.242 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 528,699 + EX31_RS23805 0.30 +0.0 528,699 + EX31_RS23805 0.30 +0.0 528,702 + EX31_RS23805 0.30 +0.6 528,702 + EX31_RS23805 0.30 +0.1 528,702 + EX31_RS23805 0.30 -0.2 528,702 + EX31_RS23805 0.30 +0.3 528,702 + EX31_RS23805 0.30 +1.4 528,702 + EX31_RS23805 0.30 +0.3 528,703 - EX31_RS23805 0.30 -0.2 528,703 - EX31_RS23805 0.30 +0.1 528,703 - EX31_RS23805 0.30 -1.6 528,703 - EX31_RS23805 0.30 +0.3 528,703 - EX31_RS23805 0.30 +1.5 528,838 + EX31_RS23805 0.45 +2.4 528,849 + EX31_RS23805 0.46 +3.0 528,849 + EX31_RS23805 0.46 +1.7 528,849 + EX31_RS23805 0.46 +0.4 528,849 + EX31_RS23805 0.46 +0.1 528,850 - EX31_RS23805 0.46 +1.0 528,858 + EX31_RS23805 0.47 +1.8 528,858 + EX31_RS23805 0.47 -1.7 528,953 + EX31_RS23805 0.57 +0.5 528,953 + EX31_RS23805 0.57 -0.2 528,954 - EX31_RS23805 0.57 +0.7 529,001 + EX31_RS23805 0.62 +1.0 529,002 - EX31_RS23805 0.62 +1.5 529,005 + EX31_RS23805 0.63 +0.5 529,006 - EX31_RS23805 0.63 +1.3 529,006 - EX31_RS23805 0.63 -0.2 529,045 + EX31_RS23805 0.67 +0.0 529,046 - EX31_RS23805 0.67 -1.0 529,062 + EX31_RS23805 0.69 -0.3 529,063 - EX31_RS23805 0.69 +0.0 529,065 + EX31_RS23805 0.69 +0.7 529,065 + EX31_RS23805 0.69 +1.3 529,066 - EX31_RS23805 0.69 -0.1 529,155 + EX31_RS23805 0.79 +1.3 529,460 - -0.7 529,518 - +0.0 529,523 - +1.0 529,575 + +0.2 529,575 + +0.0 529,576 - +0.6 529,576 - +0.8 529,578 - +0.3 529,578 - -0.3 529,578 - +0.8 529,610 + +0.9 529,736 + EX31_RS23810 0.19 +0.0 529,737 - EX31_RS23810 0.19 +0.5 529,769 - EX31_RS23810 0.24 -0.1 529,769 - EX31_RS23810 0.24 +0.9 529,769 - EX31_RS23810 0.24 +0.7 529,769 - EX31_RS23810 0.24 +0.1 529,769 - EX31_RS23810 0.24 -0.2 529,773 - EX31_RS23810 0.25 -0.1 529,794 + EX31_RS23810 0.28 +0.6 530,160 + EX31_RS23810 0.87 +0.9 530,160 + EX31_RS23810 0.87 -0.2 530,161 - EX31_RS23810 0.87 -0.1 530,161 - EX31_RS23810 0.87 +0.9 530,161 - EX31_RS23810 0.87 +1.9 530,190 + +0.3 530,208 + -0.4 530,209 - -0.2 530,223 + +0.7 530,224 - -0.4 530,224 - +0.1 530,227 + +0.0 530,227 + -0.5 530,227 + +0.3 530,227 + +1.5 530,227 + -0.2 530,227 + +0.3 530,227 + +1.8 530,227 + -0.9 530,227 + -0.3 530,227 + -0.2 530,227 + -0.9 530,228 - -0.5 530,228 - +0.1 530,228 - +0.5 530,228 - +0.4 530,228 - +1.0 530,228 - +0.9 530,228 - -1.7 530,228 - +0.8 530,228 - +0.3 530,228 - +0.6 530,228 - -0.4 530,228 - -0.5 530,235 + +0.8 530,235 + -1.0 530,236 - -0.7 530,236 - +0.3 530,236 - -0.2 530,236 - +0.4 530,246 + +0.4 530,247 - +0.4 530,247 - -2.1 530,247 - +0.4 530,247 - -0.0 530,258 + -0.8 530,272 + +0.3 530,273 - +0.3 530,295 - +0.0 530,309 + -0.0 530,316 - +0.2 530,452 + +0.4 530,485 - +0.6 530,485 - +0.6 530,485 - -0.6 530,486 + +0.2 530,486 + +0.7 530,486 + +1.0 530,486 + +0.4 530,486 + +0.3 530,486 + +1.3 530,486 + +0.8 530,486 + -1.5 530,486 + -1.9 530,486 + +0.3 530,486 + -0.7 530,486 + +0.1 530,486 + -1.5 530,486 + -0.4 530,486 + +0.7 530,486 + -1.0 530,486 + +0.4 530,486 + +0.8 530,486 + -0.1 530,486 + -0.2 530,486 + +0.5 530,486 + +0.4 530,486 + +0.6 530,486 + -0.5 530,486 + +0.7 530,486 + +0.7 530,487 - -0.6 530,487 - +0.7 530,487 - +0.4 530,487 - -0.4 530,487 - -1.7 530,487 - +0.3 530,487 - +0.0 530,487 - -2.2 530,487 - -0.1 530,487 - +1.2 530,487 - -0.7 530,487 - +1.3 530,487 - +0.3 530,487 - +1.6 530,487 - +0.3 530,487 - +0.1 530,487 - +0.6 530,487 - +1.7 530,487 - -0.2 530,487 - -0.4 530,487 - +1.3 530,487 - -1.2 530,487 - -0.8 530,488 + +0.3 530,488 + -0.2 530,488 + +1.4 530,488 + -1.0 530,488 + +0.1 530,488 + -0.4 530,488 + +0.5 530,488 + +1.5 530,488 + -1.5 530,488 + -0.7 530,488 + -0.7 530,488 + -0.1 530,488 + +0.4 530,489 - +0.1 530,489 - +0.5 530,489 - -0.5 530,489 - +0.0 530,489 - +0.5 530,489 - +0.7 530,489 - +0.2 530,489 - -1.7 530,489 - -1.3 530,489 - -0.5 530,489 - -0.2 530,492 + +0.4 530,492 + +0.8 530,492 + -0.2 530,492 + +0.7 530,492 + -0.6 530,493 - -0.0 530,493 - -0.5 530,493 - +1.1 530,493 - +1.2 530,493 - +0.7 530,493 - +1.1 530,493 - -0.3 530,496 + +0.2 530,496 + -0.6 530,496 + +2.4 530,497 - -0.2 530,508 + +1.2 530,541 + +0.8 530,580 + +0.4 530,613 + +0.7 530,613 + -0.5 530,613 + -0.6 530,613 + +1.5 530,613 + -0.5 530,613 + +0.8 530,613 + +0.6 530,613 + -0.1 530,613 + -0.3 530,613 + -1.1 530,613 + -0.9 530,613 + -0.8 530,613 + +1.0 530,614 - +0.8 530,614 - +0.6 530,614 - -1.3 530,614 - +1.4 530,614 - -1.3 530,640 - -1.2 530,662 + -0.4 530,662 + +0.3 530,695 + EX31_RS23815 0.13 -0.5 530,696 - EX31_RS23815 0.13 +0.5 530,932 - EX31_RS23815 0.50 -0.2 530,999 + EX31_RS23815 0.60 +0.3 531,003 + EX31_RS23815 0.61 +0.3 531,004 - EX31_RS23815 0.61 +0.2 531,021 - EX31_RS23815 0.64 -1.3 531,021 - EX31_RS23815 0.64 -0.2 531,021 - EX31_RS23815 0.64 +1.2 531,024 + EX31_RS23815 0.64 +0.9 531,024 + EX31_RS23815 0.64 -0.2 531,174 + EX31_RS23815 0.88 +1.2 531,241 + -0.0 531,241 + -0.9 531,241 + +0.5 531,242 - -0.7
Or see this region's nucleotide sequence