Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22155 and EX31_RS22160 are separated by 18 nucleotides EX31_RS22160 and EX31_RS22165 are separated by 5 nucleotides EX31_RS22165 and EX31_RS22170 are separated by 276 nucleotides
EX31_RS22155: EX31_RS22155 - 1-deoxy-D-xylulose-5-phosphate synthase, at 178,649 to 180,511
_RS22155
EX31_RS22160: EX31_RS22160 - (2E,6E)-farnesyl diphosphate synthase, at 180,530 to 181,459
_RS22160
EX31_RS22165: EX31_RS22165 - exodeoxyribonuclease VII small subunit, at 181,465 to 181,707
_RS22165
EX31_RS22170: EX31_RS22170 - tRNA 4-thiouridine(8) synthase ThiI, at 181,984 to 183,432
_RS22170
Position (kb) 180
181
182 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 180.523 kb on - strand at 180.523 kb on - strand at 180.526 kb on - strand at 180.526 kb on - strand at 180.526 kb on - strand at 180.532 kb on - strand at 180.532 kb on - strand at 181.708 kb on - strand at 181.719 kb on - strand at 181.719 kb on - strand at 181.719 kb on - strand at 181.719 kb on - strand at 181.750 kb on - strand at 181.750 kb on - strand at 181.842 kb on - strand at 181.848 kb on + strand at 181.848 kb on + strand at 181.848 kb on + strand at 181.848 kb on + strand at 181.849 kb on - strand at 181.849 kb on - strand at 181.849 kb on - strand at 181.849 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.867 kb on - strand at 181.867 kb on - strand at 181.870 kb on - strand at 181.870 kb on - strand at 181.876 kb on - strand at 181.876 kb on - strand at 181.915 kb on + strand at 181.916 kb on - strand at 181.916 kb on - strand at 181.916 kb on - strand at 181.918 kb on + strand at 181.918 kb on + strand at 181.919 kb on - strand at 181.926 kb on + strand at 181.926 kb on + strand at 181.926 kb on + strand at 181.927 kb on - strand at 181.935 kb on - strand at 181.946 kb on + strand at 181.946 kb on + strand at 181.946 kb on + strand at 181.947 kb on - strand at 181.947 kb on - strand at 181.947 kb on - strand at 181.947 kb on - strand at 181.947 kb on - strand at 181.950 kb on - strand at 181.969 kb on + strand at 181.969 kb on + strand at 181.992 kb on + strand at 181.992 kb on + strand at 181.993 kb on - strand at 181.998 kb on + strand at 182.052 kb on + strand at 182.053 kb on - strand at 182.053 kb on - strand at 182.064 kb on + strand at 182.064 kb on + strand at 182.064 kb on + strand at 182.064 kb on + strand at 182.064 kb on + strand at 182.064 kb on + strand at 182.145 kb on + strand, within EX31_RS22170 at 182.145 kb on + strand, within EX31_RS22170 at 182.145 kb on + strand, within EX31_RS22170 at 182.206 kb on + strand, within EX31_RS22170 at 182.328 kb on + strand, within EX31_RS22170 at 182.354 kb on + strand, within EX31_RS22170 at 182.354 kb on + strand, within EX31_RS22170 at 182.355 kb on - strand, within EX31_RS22170 at 182.362 kb on + strand, within EX31_RS22170 at 182.362 kb on + strand, within EX31_RS22170 at 182.362 kb on + strand, within EX31_RS22170 at 182.362 kb on + strand, within EX31_RS22170 at 182.363 kb on - strand, within EX31_RS22170 at 182.455 kb on - strand, within EX31_RS22170
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 180,523 - +1.3 180,523 - +0.3 180,526 - -0.6 180,526 - -1.0 180,526 - +1.5 180,532 - +1.1 180,532 - +0.4 181,708 - -0.5 181,719 - +0.4 181,719 - +0.1 181,719 - -0.3 181,719 - -0.1 181,750 - -0.2 181,750 - +0.5 181,842 - +1.1 181,848 + +1.3 181,848 + +0.8 181,848 + +3.6 181,848 + -1.6 181,849 - -0.7 181,849 - +0.3 181,849 - +1.1 181,849 - -1.7 181,851 - +0.4 181,851 - -0.3 181,851 - +0.1 181,851 - -0.5 181,851 - +0.1 181,851 - -0.7 181,851 - -1.1 181,851 - -0.8 181,851 - -0.2 181,851 - -0.4 181,851 - +0.6 181,851 - -0.6 181,851 - -1.8 181,867 - +0.4 181,867 - +0.1 181,870 - -0.5 181,870 - -1.3 181,876 - +0.4 181,876 - +1.8 181,915 + +0.2 181,916 - -1.0 181,916 - +0.2 181,916 - -0.3 181,918 + +0.0 181,918 + -0.7 181,919 - -0.4 181,926 + -0.5 181,926 + +1.6 181,926 + -0.6 181,927 - +1.6 181,935 - +1.1 181,946 + +0.6 181,946 + -1.5 181,946 + +2.3 181,947 - -0.1 181,947 - +0.3 181,947 - +2.1 181,947 - -0.9 181,947 - -1.1 181,950 - +3.1 181,969 + -0.3 181,969 + -0.3 181,992 + +1.3 181,992 + -0.6 181,993 - -0.9 181,998 + +0.8 182,052 + -0.7 182,053 - +0.1 182,053 - -0.2 182,064 + +0.5 182,064 + -0.1 182,064 + -0.2 182,064 + -0.2 182,064 + +0.3 182,064 + -1.3 182,145 + EX31_RS22170 0.11 -0.5 182,145 + EX31_RS22170 0.11 -1.9 182,145 + EX31_RS22170 0.11 -1.9 182,206 + EX31_RS22170 0.15 +1.6 182,328 + EX31_RS22170 0.24 -0.1 182,354 + EX31_RS22170 0.26 -1.8 182,354 + EX31_RS22170 0.26 +3.6 182,355 - EX31_RS22170 0.26 -2.4 182,362 + EX31_RS22170 0.26 +0.1 182,362 + EX31_RS22170 0.26 -0.1 182,362 + EX31_RS22170 0.26 -2.6 182,362 + EX31_RS22170 0.26 +1.5 182,363 - EX31_RS22170 0.26 -1.6 182,455 - EX31_RS22170 0.33 -0.5
Or see this region's nucleotide sequence