Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20865 and EX31_RS20870 are separated by 37 nucleotides EX31_RS20870 and EX31_RS20880 are separated by 632 nucleotides EX31_RS20880 and EX31_RS25605 are separated by 14 nucleotides EX31_RS25605 and EX31_RS20885 are separated by 274 nucleotides EX31_RS20885 and EX31_RS20890 overlap by 4 nucleotides
EX31_RS20865: EX31_RS20865 - bacteriocin immunity protein, at 820,671 to 820,925
_RS20865
EX31_RS20870: EX31_RS20870 - colicin, at 820,963 to 821,226
_RS20870
EX31_RS20880: EX31_RS20880 - hypothetical protein, at 821,859 to 823,301
_RS20880
EX31_RS25605: EX31_RS25605 - transposase, at 823,316 to 823,566
_RS25605
EX31_RS20885: EX31_RS20885 - conjugal transfer protein TraD, at 823,841 to 824,125
_RS20885
EX31_RS20890: EX31_RS20890 - hypothetical protein, at 824,122 to 824,379
_RS20890
Position (kb) 821
822
823
824 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 820.897 kb on + strand, within EX31_RS20865 at 820.911 kb on - strand at 820.911 kb on - strand at 820.999 kb on - strand, within EX31_RS20870 at 821.022 kb on - strand, within EX31_RS20870 at 821.026 kb on - strand, within EX31_RS20870 at 821.026 kb on - strand, within EX31_RS20870 at 821.096 kb on + strand, within EX31_RS20870 at 821.096 kb on + strand, within EX31_RS20870 at 821.100 kb on + strand, within EX31_RS20870 at 821.100 kb on + strand, within EX31_RS20870 at 821.100 kb on + strand, within EX31_RS20870 at 821.101 kb on - strand, within EX31_RS20870 at 821.101 kb on - strand, within EX31_RS20870 at 821.101 kb on - strand, within EX31_RS20870 at 821.140 kb on + strand, within EX31_RS20870 at 821.150 kb on + strand, within EX31_RS20870 at 821.150 kb on + strand, within EX31_RS20870 at 821.191 kb on - strand, within EX31_RS20870 at 821.365 kb on + strand at 821.366 kb on - strand at 821.366 kb on - strand at 821.366 kb on - strand at 821.366 kb on - strand at 821.408 kb on + strand at 821.409 kb on - strand at 821.409 kb on - strand at 821.454 kb on - strand at 821.485 kb on - strand at 821.561 kb on - strand at 821.561 kb on - strand at 821.561 kb on - strand at 821.563 kb on + strand at 821.563 kb on + strand at 821.563 kb on + strand at 821.563 kb on + strand at 821.564 kb on - strand at 821.564 kb on - strand at 821.564 kb on - strand at 821.573 kb on - strand at 821.592 kb on + strand at 821.592 kb on + strand at 821.593 kb on - strand at 821.593 kb on - strand at 821.593 kb on - strand at 821.606 kb on + strand at 821.628 kb on + strand at 821.628 kb on + strand at 821.635 kb on + strand at 821.648 kb on - strand at 821.648 kb on - strand at 821.661 kb on - strand at 821.671 kb on + strand at 821.671 kb on + strand at 821.671 kb on + strand at 821.671 kb on + strand at 821.671 kb on + strand at 821.672 kb on - strand at 821.672 kb on - strand at 821.673 kb on + strand at 821.673 kb on + strand at 821.673 kb on + strand at 821.673 kb on + strand at 821.673 kb on + strand at 821.673 kb on + strand at 821.673 kb on + strand at 821.673 kb on + strand at 821.673 kb on + strand at 821.674 kb on - strand at 821.674 kb on - strand at 821.674 kb on - strand at 821.674 kb on - strand at 821.674 kb on - strand at 821.675 kb on + strand at 821.675 kb on + strand at 821.675 kb on + strand at 821.675 kb on + strand at 821.675 kb on + strand at 821.676 kb on - strand at 821.676 kb on - strand at 821.676 kb on - strand at 821.676 kb on - strand at 821.749 kb on + strand at 821.749 kb on + strand at 821.749 kb on + strand at 821.749 kb on + strand at 821.749 kb on + strand at 821.749 kb on + strand at 821.797 kb on - strand at 821.855 kb on - strand at 821.856 kb on + strand at 822.090 kb on - strand, within EX31_RS20880 at 822.245 kb on + strand, within EX31_RS20880 at 822.245 kb on + strand, within EX31_RS20880 at 822.597 kb on - strand, within EX31_RS20880 at 822.940 kb on + strand, within EX31_RS20880 at 822.955 kb on - strand, within EX31_RS20880 at 822.983 kb on + strand, within EX31_RS20880 at 822.983 kb on + strand, within EX31_RS20880 at 822.984 kb on - strand, within EX31_RS20880 at 822.985 kb on + strand, within EX31_RS20880 at 822.986 kb on - strand, within EX31_RS20880 at 823.331 kb on - strand at 823.366 kb on + strand, within EX31_RS25605 at 823.451 kb on + strand, within EX31_RS25605 at 823.483 kb on + strand, within EX31_RS25605 at 823.483 kb on + strand, within EX31_RS25605 at 823.484 kb on - strand, within EX31_RS25605 at 823.486 kb on - strand, within EX31_RS25605 at 823.488 kb on + strand, within EX31_RS25605 at 823.488 kb on + strand, within EX31_RS25605 at 823.544 kb on + strand at 823.544 kb on + strand at 823.545 kb on - strand at 823.592 kb on - strand at 823.592 kb on - strand at 823.634 kb on + strand at 823.645 kb on + strand at 823.646 kb on - strand at 823.648 kb on + strand at 823.794 kb on - strand at 823.794 kb on - strand at 823.842 kb on + strand at 823.842 kb on + strand at 823.842 kb on + strand at 823.843 kb on - strand at 823.875 kb on + strand, within EX31_RS20885 at 823.875 kb on + strand, within EX31_RS20885 at 823.875 kb on + strand, within EX31_RS20885 at 823.876 kb on - strand, within EX31_RS20885 at 823.876 kb on - strand, within EX31_RS20885 at 823.931 kb on - strand, within EX31_RS20885 at 823.934 kb on - strand, within EX31_RS20885 at 823.934 kb on - strand, within EX31_RS20885 at 823.965 kb on - strand, within EX31_RS20885 at 823.968 kb on + strand, within EX31_RS20885 at 823.969 kb on - strand, within EX31_RS20885 at 823.972 kb on + strand, within EX31_RS20885 at 823.973 kb on - strand, within EX31_RS20885 at 823.973 kb on - strand, within EX31_RS20885 at 824.074 kb on - strand, within EX31_RS20885 at 824.235 kb on + strand, within EX31_RS20890 at 824.236 kb on - strand, within EX31_RS20890
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 820,897 + EX31_RS20865 0.89 +0.3 820,911 - +0.3 820,911 - -0.2 820,999 - EX31_RS20870 0.14 +2.5 821,022 - EX31_RS20870 0.22 +1.6 821,026 - EX31_RS20870 0.24 +0.6 821,026 - EX31_RS20870 0.24 +0.4 821,096 + EX31_RS20870 0.50 +0.1 821,096 + EX31_RS20870 0.50 +1.6 821,100 + EX31_RS20870 0.52 -0.6 821,100 + EX31_RS20870 0.52 +0.9 821,100 + EX31_RS20870 0.52 +2.0 821,101 - EX31_RS20870 0.52 +0.3 821,101 - EX31_RS20870 0.52 +0.6 821,101 - EX31_RS20870 0.52 +1.7 821,140 + EX31_RS20870 0.67 +1.6 821,150 + EX31_RS20870 0.71 +1.1 821,150 + EX31_RS20870 0.71 -1.5 821,191 - EX31_RS20870 0.86 +0.2 821,365 + +0.8 821,366 - +0.8 821,366 - +0.9 821,366 - -0.0 821,366 - -0.3 821,408 + -0.6 821,409 - -0.3 821,409 - -0.4 821,454 - +0.3 821,485 - +0.6 821,561 - -0.0 821,561 - +1.0 821,561 - +0.2 821,563 + -0.6 821,563 + +0.6 821,563 + -0.6 821,563 + +0.4 821,564 - -0.6 821,564 - -1.8 821,564 - +0.4 821,573 - +1.5 821,592 + +0.5 821,592 + +0.6 821,593 - +0.3 821,593 - -0.1 821,593 - +0.2 821,606 + -0.6 821,628 + +0.5 821,628 + +1.3 821,635 + +0.4 821,648 - -1.3 821,648 - -1.0 821,661 - +4.4 821,671 + -0.4 821,671 + +0.8 821,671 + +0.5 821,671 + +1.1 821,671 + -0.3 821,672 - -0.1 821,672 - +0.4 821,673 + +0.8 821,673 + +0.6 821,673 + +0.2 821,673 + +0.7 821,673 + +0.4 821,673 + +0.0 821,673 + -0.2 821,673 + +0.1 821,673 + +1.5 821,674 - +1.5 821,674 - -0.1 821,674 - -0.7 821,674 - +1.7 821,674 - -0.1 821,675 + +0.4 821,675 + +0.7 821,675 + +0.2 821,675 + -0.5 821,675 + +0.4 821,676 - +0.3 821,676 - -0.5 821,676 - -0.4 821,676 - +1.9 821,749 + +0.7 821,749 + +0.6 821,749 + +0.2 821,749 + +1.1 821,749 + +0.4 821,749 + +0.2 821,797 - +0.8 821,855 - +0.8 821,856 + -0.5 822,090 - EX31_RS20880 0.16 -0.6 822,245 + EX31_RS20880 0.27 +1.1 822,245 + EX31_RS20880 0.27 +0.5 822,597 - EX31_RS20880 0.51 +0.3 822,940 + EX31_RS20880 0.75 +0.4 822,955 - EX31_RS20880 0.76 -1.1 822,983 + EX31_RS20880 0.78 +1.2 822,983 + EX31_RS20880 0.78 +1.8 822,984 - EX31_RS20880 0.78 +0.4 822,985 + EX31_RS20880 0.78 +0.5 822,986 - EX31_RS20880 0.78 -1.6 823,331 - +1.0 823,366 + EX31_RS25605 0.20 +0.4 823,451 + EX31_RS25605 0.54 +0.4 823,483 + EX31_RS25605 0.67 +0.4 823,483 + EX31_RS25605 0.67 +0.7 823,484 - EX31_RS25605 0.67 -0.9 823,486 - EX31_RS25605 0.68 -1.1 823,488 + EX31_RS25605 0.69 +0.9 823,488 + EX31_RS25605 0.69 +0.9 823,544 + +1.8 823,544 + +1.4 823,545 - +2.2 823,592 - -1.5 823,592 - +0.5 823,634 + +2.1 823,645 + -0.1 823,646 - -2.5 823,648 + -1.6 823,794 - +1.0 823,794 - +0.4 823,842 + -2.3 823,842 + -0.1 823,842 + +0.1 823,843 - -0.1 823,875 + EX31_RS20885 0.12 +1.0 823,875 + EX31_RS20885 0.12 +1.9 823,875 + EX31_RS20885 0.12 +0.5 823,876 - EX31_RS20885 0.12 -0.6 823,876 - EX31_RS20885 0.12 -0.1 823,931 - EX31_RS20885 0.32 -0.2 823,934 - EX31_RS20885 0.33 -0.1 823,934 - EX31_RS20885 0.33 +0.8 823,965 - EX31_RS20885 0.44 +1.6 823,968 + EX31_RS20885 0.45 +1.6 823,969 - EX31_RS20885 0.45 -0.6 823,972 + EX31_RS20885 0.46 +0.9 823,973 - EX31_RS20885 0.46 -0.2 823,973 - EX31_RS20885 0.46 -1.1 824,074 - EX31_RS20885 0.82 +0.5 824,235 + EX31_RS20890 0.44 +0.7 824,236 - EX31_RS20890 0.44 -0.1
Or see this region's nucleotide sequence