Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20650 and EX31_RS20655 are separated by 135 nucleotides EX31_RS20655 and EX31_RS20660 are separated by 37 nucleotides
EX31_RS20650: EX31_RS20650 - OFA family MFS transporter, at 771,679 to 772,929
_RS20650
EX31_RS20655: EX31_RS20655 - hypothetical protein, at 773,065 to 773,268
_RS20655
EX31_RS20660: EX31_RS20660 - glucarate dehydratase, at 773,306 to 774,652
_RS20660
Position (kb)
773
774 Strain fitness (log2 ratio)
-1
0
1
2 at 772.140 kb on - strand, within EX31_RS20650 at 772.364 kb on - strand, within EX31_RS20650 at 772.364 kb on - strand, within EX31_RS20650 at 772.364 kb on - strand, within EX31_RS20650 at 772.411 kb on + strand, within EX31_RS20650 at 772.411 kb on + strand, within EX31_RS20650 at 772.411 kb on + strand, within EX31_RS20650 at 772.411 kb on + strand, within EX31_RS20650 at 772.411 kb on + strand, within EX31_RS20650 at 772.411 kb on + strand, within EX31_RS20650 at 772.411 kb on + strand, within EX31_RS20650 at 772.412 kb on - strand, within EX31_RS20650 at 772.412 kb on - strand, within EX31_RS20650 at 772.412 kb on - strand, within EX31_RS20650 at 772.412 kb on - strand, within EX31_RS20650 at 772.412 kb on - strand, within EX31_RS20650 at 772.564 kb on - strand, within EX31_RS20650 at 772.691 kb on - strand, within EX31_RS20650 at 772.841 kb on + strand at 772.841 kb on + strand at 772.841 kb on + strand at 772.841 kb on + strand at 772.841 kb on + strand at 772.842 kb on - strand at 772.842 kb on - strand at 772.842 kb on - strand at 772.842 kb on - strand at 772.842 kb on - strand at 772.844 kb on + strand at 772.844 kb on + strand at 772.844 kb on + strand at 772.845 kb on - strand at 772.845 kb on - strand at 772.845 kb on - strand at 772.890 kb on + strand at 772.891 kb on - strand at 772.908 kb on + strand at 772.908 kb on + strand at 772.908 kb on + strand at 772.909 kb on - strand at 772.911 kb on + strand at 772.911 kb on + strand at 772.911 kb on + strand at 772.912 kb on - strand at 772.927 kb on + strand at 772.927 kb on + strand at 772.927 kb on + strand at 772.927 kb on + strand at 772.928 kb on - strand at 772.928 kb on - strand at 772.928 kb on - strand at 772.950 kb on + strand at 772.950 kb on + strand at 772.950 kb on + strand at 772.950 kb on + strand at 772.950 kb on + strand at 772.950 kb on + strand at 772.950 kb on + strand at 772.950 kb on + strand at 772.950 kb on + strand at 772.950 kb on + strand at 772.950 kb on + strand at 772.950 kb on + strand at 772.950 kb on + strand at 772.950 kb on + strand at 772.951 kb on - strand at 772.951 kb on - strand at 772.951 kb on - strand at 772.951 kb on - strand at 772.951 kb on - strand at 772.951 kb on - strand at 772.951 kb on - strand at 772.951 kb on - strand at 772.973 kb on + strand at 772.973 kb on + strand at 772.973 kb on + strand at 772.973 kb on + strand at 772.973 kb on + strand at 772.973 kb on + strand at 772.973 kb on + strand at 772.973 kb on + strand at 772.973 kb on + strand at 772.973 kb on + strand at 772.974 kb on - strand at 772.974 kb on - strand at 772.974 kb on - strand at 772.974 kb on - strand at 772.974 kb on - strand at 772.974 kb on - strand at 772.987 kb on + strand at 772.988 kb on - strand at 772.988 kb on - strand at 773.002 kb on + strand at 773.002 kb on + strand at 773.003 kb on - strand at 773.003 kb on - strand at 773.003 kb on - strand at 773.025 kb on - strand at 773.030 kb on + strand at 773.030 kb on + strand at 773.031 kb on - strand at 773.076 kb on + strand at 773.214 kb on + strand, within EX31_RS20655 at 773.232 kb on + strand, within EX31_RS20655 at 773.233 kb on - strand, within EX31_RS20655 at 773.267 kb on - strand at 773.277 kb on + strand at 773.277 kb on + strand at 773.278 kb on - strand at 773.278 kb on - strand at 773.278 kb on - strand at 773.278 kb on - strand at 773.358 kb on + strand at 773.358 kb on + strand at 773.359 kb on - strand at 773.406 kb on + strand at 773.407 kb on - strand at 773.407 kb on - strand at 773.407 kb on - strand at 773.545 kb on + strand, within EX31_RS20660 at 773.545 kb on + strand, within EX31_RS20660 at 773.656 kb on + strand, within EX31_RS20660 at 773.656 kb on + strand, within EX31_RS20660 at 773.656 kb on + strand, within EX31_RS20660 at 773.656 kb on + strand, within EX31_RS20660 at 773.656 kb on + strand, within EX31_RS20660 at 773.656 kb on + strand, within EX31_RS20660 at 773.656 kb on + strand, within EX31_RS20660 at 773.656 kb on + strand, within EX31_RS20660 at 773.656 kb on + strand, within EX31_RS20660 at 773.657 kb on - strand, within EX31_RS20660 at 773.657 kb on - strand, within EX31_RS20660 at 773.657 kb on - strand, within EX31_RS20660 at 773.657 kb on - strand, within EX31_RS20660 at 773.660 kb on + strand, within EX31_RS20660 at 773.660 kb on + strand, within EX31_RS20660 at 773.660 kb on + strand, within EX31_RS20660 at 773.660 kb on + strand, within EX31_RS20660 at 773.660 kb on + strand, within EX31_RS20660 at 773.660 kb on + strand, within EX31_RS20660 at 773.660 kb on + strand, within EX31_RS20660 at 773.660 kb on + strand, within EX31_RS20660 at 773.660 kb on + strand, within EX31_RS20660 at 773.660 kb on + strand, within EX31_RS20660 at 773.661 kb on - strand, within EX31_RS20660 at 773.661 kb on - strand, within EX31_RS20660 at 773.661 kb on - strand, within EX31_RS20660 at 773.661 kb on - strand, within EX31_RS20660 at 773.661 kb on - strand, within EX31_RS20660 at 773.661 kb on - strand, within EX31_RS20660 at 773.661 kb on - strand, within EX31_RS20660 at 773.661 kb on - strand, within EX31_RS20660 at 773.674 kb on + strand, within EX31_RS20660 at 773.776 kb on + strand, within EX31_RS20660 at 773.776 kb on + strand, within EX31_RS20660 at 773.871 kb on + strand, within EX31_RS20660 at 773.871 kb on + strand, within EX31_RS20660 at 773.872 kb on - strand, within EX31_RS20660 at 773.872 kb on - strand, within EX31_RS20660 at 773.872 kb on - strand, within EX31_RS20660 at 773.993 kb on + strand, within EX31_RS20660 at 773.994 kb on - strand, within EX31_RS20660 at 773.994 kb on - strand, within EX31_RS20660 at 774.025 kb on + strand, within EX31_RS20660 at 774.025 kb on + strand, within EX31_RS20660 at 774.025 kb on + strand, within EX31_RS20660 at 774.041 kb on - strand, within EX31_RS20660 at 774.041 kb on - strand, within EX31_RS20660 at 774.048 kb on + strand, within EX31_RS20660 at 774.048 kb on + strand, within EX31_RS20660 at 774.048 kb on + strand, within EX31_RS20660 at 774.048 kb on + strand, within EX31_RS20660 at 774.049 kb on - strand, within EX31_RS20660 at 774.052 kb on + strand, within EX31_RS20660 at 774.053 kb on - strand, within EX31_RS20660 at 774.053 kb on - strand, within EX31_RS20660 at 774.053 kb on - strand, within EX31_RS20660 at 774.053 kb on - strand, within EX31_RS20660 at 774.190 kb on + strand, within EX31_RS20660 at 774.192 kb on + strand, within EX31_RS20660 at 774.192 kb on + strand, within EX31_RS20660 at 774.192 kb on + strand, within EX31_RS20660 at 774.193 kb on - strand, within EX31_RS20660 at 774.193 kb on - strand, within EX31_RS20660 at 774.193 kb on - strand, within EX31_RS20660 at 774.193 kb on - strand, within EX31_RS20660 at 774.193 kb on - strand, within EX31_RS20660 at 774.202 kb on - strand, within EX31_RS20660
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 772,140 - EX31_RS20650 0.37 +1.2 772,364 - EX31_RS20650 0.55 +0.7 772,364 - EX31_RS20650 0.55 +1.5 772,364 - EX31_RS20650 0.55 -0.3 772,411 + EX31_RS20650 0.59 +0.7 772,411 + EX31_RS20650 0.59 -1.4 772,411 + EX31_RS20650 0.59 -0.8 772,411 + EX31_RS20650 0.59 +0.5 772,411 + EX31_RS20650 0.59 +0.1 772,411 + EX31_RS20650 0.59 -0.8 772,411 + EX31_RS20650 0.59 +1.6 772,412 - EX31_RS20650 0.59 +1.7 772,412 - EX31_RS20650 0.59 +1.1 772,412 - EX31_RS20650 0.59 -1.0 772,412 - EX31_RS20650 0.59 -0.4 772,412 - EX31_RS20650 0.59 +0.1 772,564 - EX31_RS20650 0.71 -1.4 772,691 - EX31_RS20650 0.81 +0.1 772,841 + +0.4 772,841 + -0.4 772,841 + -0.9 772,841 + +0.3 772,841 + -1.4 772,842 - +0.0 772,842 - +0.9 772,842 - +0.0 772,842 - -0.7 772,842 - -0.1 772,844 + +0.2 772,844 + -1.0 772,844 + -1.6 772,845 - +1.0 772,845 - -0.1 772,845 - -0.1 772,890 + +0.7 772,891 - +0.3 772,908 + +0.9 772,908 + +0.1 772,908 + -0.1 772,909 - +1.2 772,911 + +0.4 772,911 + -0.4 772,911 + -0.1 772,912 - +0.5 772,927 + +0.3 772,927 + +0.2 772,927 + +0.3 772,927 + -0.2 772,928 - -1.5 772,928 - -0.1 772,928 - -0.3 772,950 + +0.5 772,950 + +0.8 772,950 + +0.5 772,950 + -0.2 772,950 + +0.4 772,950 + -1.4 772,950 + +0.9 772,950 + +1.2 772,950 + -0.2 772,950 + +0.5 772,950 + +0.5 772,950 + -0.5 772,950 + -1.1 772,950 + +1.2 772,951 - -0.3 772,951 - +0.7 772,951 - -0.3 772,951 - -1.5 772,951 - -0.3 772,951 - -0.1 772,951 - +0.1 772,951 - +0.3 772,973 + +0.8 772,973 + +0.3 772,973 + +0.6 772,973 + -0.2 772,973 + +1.5 772,973 + -0.1 772,973 + -1.7 772,973 + -0.3 772,973 + -0.0 772,973 + +0.4 772,974 - -0.1 772,974 - -0.0 772,974 - -0.4 772,974 - -0.3 772,974 - +0.9 772,974 - -0.5 772,987 + +0.3 772,988 - -0.2 772,988 - +0.3 773,002 + +0.8 773,002 + +0.9 773,003 - -0.0 773,003 - +0.6 773,003 - +0.5 773,025 - +1.3 773,030 + -0.3 773,030 + +0.1 773,031 - +0.4 773,076 + -0.3 773,214 + EX31_RS20655 0.73 -0.7 773,232 + EX31_RS20655 0.82 -0.7 773,233 - EX31_RS20655 0.82 -1.2 773,267 - +1.0 773,277 + -0.3 773,277 + +1.4 773,278 - -0.5 773,278 - +0.4 773,278 - +0.2 773,278 - +0.5 773,358 + -0.0 773,358 + -1.6 773,359 - +1.4 773,406 + -0.1 773,407 - +1.0 773,407 - +0.7 773,407 - -1.3 773,545 + EX31_RS20660 0.18 -0.6 773,545 + EX31_RS20660 0.18 -0.8 773,656 + EX31_RS20660 0.26 -0.5 773,656 + EX31_RS20660 0.26 -0.6 773,656 + EX31_RS20660 0.26 -0.4 773,656 + EX31_RS20660 0.26 +0.3 773,656 + EX31_RS20660 0.26 +2.2 773,656 + EX31_RS20660 0.26 +1.0 773,656 + EX31_RS20660 0.26 -0.3 773,656 + EX31_RS20660 0.26 -0.4 773,656 + EX31_RS20660 0.26 -0.1 773,657 - EX31_RS20660 0.26 +0.3 773,657 - EX31_RS20660 0.26 -1.6 773,657 - EX31_RS20660 0.26 +0.3 773,657 - EX31_RS20660 0.26 +0.2 773,660 + EX31_RS20660 0.26 -0.5 773,660 + EX31_RS20660 0.26 -0.0 773,660 + EX31_RS20660 0.26 +0.3 773,660 + EX31_RS20660 0.26 +0.9 773,660 + EX31_RS20660 0.26 +0.6 773,660 + EX31_RS20660 0.26 -0.7 773,660 + EX31_RS20660 0.26 +0.3 773,660 + EX31_RS20660 0.26 -0.6 773,660 + EX31_RS20660 0.26 -0.1 773,660 + EX31_RS20660 0.26 +0.5 773,661 - EX31_RS20660 0.26 -0.3 773,661 - EX31_RS20660 0.26 +1.5 773,661 - EX31_RS20660 0.26 +0.8 773,661 - EX31_RS20660 0.26 -0.7 773,661 - EX31_RS20660 0.26 +0.5 773,661 - EX31_RS20660 0.26 -1.2 773,661 - EX31_RS20660 0.26 -1.2 773,661 - EX31_RS20660 0.26 -0.8 773,674 + EX31_RS20660 0.27 +0.6 773,776 + EX31_RS20660 0.35 +0.4 773,776 + EX31_RS20660 0.35 +0.1 773,871 + EX31_RS20660 0.42 -1.6 773,871 + EX31_RS20660 0.42 +0.9 773,872 - EX31_RS20660 0.42 +0.8 773,872 - EX31_RS20660 0.42 +0.1 773,872 - EX31_RS20660 0.42 +0.4 773,993 + EX31_RS20660 0.51 +0.7 773,994 - EX31_RS20660 0.51 -0.1 773,994 - EX31_RS20660 0.51 +0.1 774,025 + EX31_RS20660 0.53 -0.9 774,025 + EX31_RS20660 0.53 -1.5 774,025 + EX31_RS20660 0.53 -0.5 774,041 - EX31_RS20660 0.55 +0.2 774,041 - EX31_RS20660 0.55 -0.1 774,048 + EX31_RS20660 0.55 +0.5 774,048 + EX31_RS20660 0.55 -0.4 774,048 + EX31_RS20660 0.55 +0.7 774,048 + EX31_RS20660 0.55 -0.4 774,049 - EX31_RS20660 0.55 +1.5 774,052 + EX31_RS20660 0.55 +1.5 774,053 - EX31_RS20660 0.55 +0.0 774,053 - EX31_RS20660 0.55 +0.6 774,053 - EX31_RS20660 0.55 -0.2 774,053 - EX31_RS20660 0.55 -0.6 774,190 + EX31_RS20660 0.66 +1.2 774,192 + EX31_RS20660 0.66 -0.1 774,192 + EX31_RS20660 0.66 -0.3 774,192 + EX31_RS20660 0.66 +0.3 774,193 - EX31_RS20660 0.66 -0.0 774,193 - EX31_RS20660 0.66 -0.7 774,193 - EX31_RS20660 0.66 +0.2 774,193 - EX31_RS20660 0.66 +0.8 774,193 - EX31_RS20660 0.66 -0.1 774,202 - EX31_RS20660 0.67 -0.9
Or see this region's nucleotide sequence