Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20185 and EX31_RS20190 are separated by 48 nucleotides EX31_RS20190 and EX31_RS20195 are separated by 56 nucleotides
EX31_RS20185: EX31_RS20185 - phosphoenolpyruvate-protein phosphotransferase PtsI, at 665,114 to 666,841
_RS20185
EX31_RS20190: EX31_RS20190 - PTS glucose transporter subunit IIA, at 666,890 to 667,399
_RS20190
EX31_RS20195: EX31_RS20195 - HAMP domain-containing protein, at 667,456 to 668,805
_RS20195
Position (kb)
666
667
668 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 666.062 kb on + strand, within EX31_RS20185 at 666.062 kb on + strand, within EX31_RS20185 at 666.064 kb on + strand, within EX31_RS20185 at 666.105 kb on + strand, within EX31_RS20185 at 666.105 kb on + strand, within EX31_RS20185 at 666.143 kb on + strand, within EX31_RS20185 at 666.143 kb on + strand, within EX31_RS20185 at 666.144 kb on - strand, within EX31_RS20185 at 666.196 kb on + strand, within EX31_RS20185 at 666.490 kb on + strand, within EX31_RS20185 at 666.490 kb on + strand, within EX31_RS20185 at 666.490 kb on + strand, within EX31_RS20185 at 666.491 kb on - strand, within EX31_RS20185 at 666.533 kb on + strand, within EX31_RS20185 at 666.533 kb on + strand, within EX31_RS20185 at 666.555 kb on + strand, within EX31_RS20185 at 666.568 kb on + strand, within EX31_RS20185 at 666.568 kb on + strand, within EX31_RS20185 at 666.578 kb on - strand, within EX31_RS20185 at 666.646 kb on + strand, within EX31_RS20185 at 666.646 kb on + strand, within EX31_RS20185 at 666.646 kb on + strand, within EX31_RS20185 at 666.657 kb on + strand, within EX31_RS20185 at 666.672 kb on + strand at 666.694 kb on + strand at 666.694 kb on + strand at 666.781 kb on + strand at 666.839 kb on + strand at 666.856 kb on + strand at 666.856 kb on + strand at 666.871 kb on + strand at 666.878 kb on + strand at 666.949 kb on + strand, within EX31_RS20190 at 667.045 kb on + strand, within EX31_RS20190 at 667.046 kb on - strand, within EX31_RS20190 at 667.315 kb on + strand, within EX31_RS20190 at 667.354 kb on + strand at 667.397 kb on + strand at 667.397 kb on + strand at 667.397 kb on + strand at 667.397 kb on + strand at 667.416 kb on + strand at 667.416 kb on + strand at 667.416 kb on + strand at 667.522 kb on + strand at 667.522 kb on + strand at 667.523 kb on - strand at 667.523 kb on - strand at 667.523 kb on - strand at 667.523 kb on - strand at 667.523 kb on - strand at 667.523 kb on - strand at 667.627 kb on - strand, within EX31_RS20195 at 667.639 kb on - strand, within EX31_RS20195 at 667.639 kb on - strand, within EX31_RS20195 at 667.639 kb on - strand, within EX31_RS20195 at 667.639 kb on - strand, within EX31_RS20195 at 667.639 kb on - strand, within EX31_RS20195 at 667.686 kb on - strand, within EX31_RS20195 at 667.686 kb on - strand, within EX31_RS20195 at 667.686 kb on - strand, within EX31_RS20195 at 667.686 kb on - strand, within EX31_RS20195 at 667.686 kb on - strand, within EX31_RS20195 at 667.716 kb on + strand, within EX31_RS20195 at 667.717 kb on - strand, within EX31_RS20195 at 667.717 kb on - strand, within EX31_RS20195 at 667.717 kb on - strand, within EX31_RS20195 at 667.717 kb on - strand, within EX31_RS20195 at 667.717 kb on - strand, within EX31_RS20195 at 667.717 kb on - strand, within EX31_RS20195 at 667.749 kb on + strand, within EX31_RS20195 at 667.749 kb on + strand, within EX31_RS20195 at 667.750 kb on - strand, within EX31_RS20195 at 667.791 kb on + strand, within EX31_RS20195 at 667.792 kb on - strand, within EX31_RS20195 at 667.805 kb on + strand, within EX31_RS20195 at 667.806 kb on - strand, within EX31_RS20195 at 667.806 kb on - strand, within EX31_RS20195 at 667.812 kb on + strand, within EX31_RS20195 at 667.812 kb on + strand, within EX31_RS20195 at 667.812 kb on + strand, within EX31_RS20195 at 667.813 kb on - strand, within EX31_RS20195 at 667.813 kb on - strand, within EX31_RS20195 at 667.916 kb on + strand, within EX31_RS20195 at 667.948 kb on - strand, within EX31_RS20195 at 668.005 kb on + strand, within EX31_RS20195 at 668.006 kb on - strand, within EX31_RS20195 at 668.006 kb on - strand, within EX31_RS20195 at 668.006 kb on - strand, within EX31_RS20195 at 668.006 kb on - strand, within EX31_RS20195 at 668.006 kb on - strand, within EX31_RS20195 at 668.006 kb on - strand, within EX31_RS20195 at 668.006 kb on - strand, within EX31_RS20195 at 668.158 kb on - strand, within EX31_RS20195 at 668.158 kb on - strand, within EX31_RS20195 at 668.158 kb on - strand, within EX31_RS20195 at 668.158 kb on - strand, within EX31_RS20195 at 668.158 kb on - strand, within EX31_RS20195 at 668.214 kb on + strand, within EX31_RS20195 at 668.231 kb on - strand, within EX31_RS20195 at 668.263 kb on - strand, within EX31_RS20195 at 668.263 kb on - strand, within EX31_RS20195 at 668.302 kb on + strand, within EX31_RS20195 at 668.303 kb on - strand, within EX31_RS20195 at 668.318 kb on - strand, within EX31_RS20195 at 668.359 kb on - strand, within EX31_RS20195 at 668.391 kb on - strand, within EX31_RS20195
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 666,062 + EX31_RS20185 0.55 +0.6 666,062 + EX31_RS20185 0.55 +0.4 666,064 + EX31_RS20185 0.55 -1.2 666,105 + EX31_RS20185 0.57 +0.5 666,105 + EX31_RS20185 0.57 +1.3 666,143 + EX31_RS20185 0.60 +0.9 666,143 + EX31_RS20185 0.60 -0.2 666,144 - EX31_RS20185 0.60 +1.7 666,196 + EX31_RS20185 0.63 +0.2 666,490 + EX31_RS20185 0.80 -0.6 666,490 + EX31_RS20185 0.80 -1.9 666,490 + EX31_RS20185 0.80 +1.0 666,491 - EX31_RS20185 0.80 -0.8 666,533 + EX31_RS20185 0.82 +0.7 666,533 + EX31_RS20185 0.82 +0.3 666,555 + EX31_RS20185 0.83 +0.2 666,568 + EX31_RS20185 0.84 +2.2 666,568 + EX31_RS20185 0.84 +1.3 666,578 - EX31_RS20185 0.85 +1.0 666,646 + EX31_RS20185 0.89 +0.7 666,646 + EX31_RS20185 0.89 +0.3 666,646 + EX31_RS20185 0.89 -0.8 666,657 + EX31_RS20185 0.89 -0.4 666,672 + -0.8 666,694 + -0.2 666,694 + +0.5 666,781 + +0.7 666,839 + -0.2 666,856 + -0.0 666,856 + -0.4 666,871 + -0.2 666,878 + -0.8 666,949 + EX31_RS20190 0.12 -0.2 667,045 + EX31_RS20190 0.30 +0.6 667,046 - EX31_RS20190 0.31 -0.8 667,315 + EX31_RS20190 0.83 +0.0 667,354 + +1.7 667,397 + +0.1 667,397 + +0.6 667,397 + -0.1 667,397 + +0.4 667,416 + +1.4 667,416 + -0.8 667,416 + +1.0 667,522 + -0.1 667,522 + +1.4 667,523 - -1.5 667,523 - +0.2 667,523 - -1.1 667,523 - -0.0 667,523 - -0.5 667,523 - -0.4 667,627 - EX31_RS20195 0.13 +1.2 667,639 - EX31_RS20195 0.14 +0.3 667,639 - EX31_RS20195 0.14 +0.4 667,639 - EX31_RS20195 0.14 +0.8 667,639 - EX31_RS20195 0.14 -0.3 667,639 - EX31_RS20195 0.14 +0.9 667,686 - EX31_RS20195 0.17 -1.6 667,686 - EX31_RS20195 0.17 +0.3 667,686 - EX31_RS20195 0.17 +0.9 667,686 - EX31_RS20195 0.17 -0.1 667,686 - EX31_RS20195 0.17 -0.7 667,716 + EX31_RS20195 0.19 +1.3 667,717 - EX31_RS20195 0.19 -0.2 667,717 - EX31_RS20195 0.19 -0.3 667,717 - EX31_RS20195 0.19 +0.6 667,717 - EX31_RS20195 0.19 -0.4 667,717 - EX31_RS20195 0.19 +1.1 667,717 - EX31_RS20195 0.19 -0.2 667,749 + EX31_RS20195 0.22 +0.4 667,749 + EX31_RS20195 0.22 +0.4 667,750 - EX31_RS20195 0.22 -0.6 667,791 + EX31_RS20195 0.25 +0.6 667,792 - EX31_RS20195 0.25 -0.0 667,805 + EX31_RS20195 0.26 +1.4 667,806 - EX31_RS20195 0.26 +1.0 667,806 - EX31_RS20195 0.26 -0.4 667,812 + EX31_RS20195 0.26 -0.2 667,812 + EX31_RS20195 0.26 -3.1 667,812 + EX31_RS20195 0.26 -0.2 667,813 - EX31_RS20195 0.26 -0.2 667,813 - EX31_RS20195 0.26 +0.9 667,916 + EX31_RS20195 0.34 -2.2 667,948 - EX31_RS20195 0.36 -0.9 668,005 + EX31_RS20195 0.41 +0.4 668,006 - EX31_RS20195 0.41 +1.5 668,006 - EX31_RS20195 0.41 +1.0 668,006 - EX31_RS20195 0.41 -1.0 668,006 - EX31_RS20195 0.41 -0.3 668,006 - EX31_RS20195 0.41 +1.8 668,006 - EX31_RS20195 0.41 +0.9 668,006 - EX31_RS20195 0.41 +0.6 668,158 - EX31_RS20195 0.52 +0.1 668,158 - EX31_RS20195 0.52 +1.4 668,158 - EX31_RS20195 0.52 +1.0 668,158 - EX31_RS20195 0.52 +0.1 668,158 - EX31_RS20195 0.52 +0.5 668,214 + EX31_RS20195 0.56 +2.2 668,231 - EX31_RS20195 0.57 -0.4 668,263 - EX31_RS20195 0.60 -0.2 668,263 - EX31_RS20195 0.60 -0.2 668,302 + EX31_RS20195 0.63 +1.8 668,303 - EX31_RS20195 0.63 +0.8 668,318 - EX31_RS20195 0.64 +1.9 668,359 - EX31_RS20195 0.67 +0.2 668,391 - EX31_RS20195 0.69 -0.2
Or see this region's nucleotide sequence