Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS19395 and EX31_RS19400 are separated by 12 nucleotides EX31_RS19400 and EX31_RS19405 are separated by 14 nucleotides EX31_RS19405 and EX31_RS19410 overlap by 4 nucleotides
EX31_RS19395: EX31_RS19395 - NADH-quinone oxidoreductase subunit J, at 506,394 to 506,954
_RS19395
EX31_RS19400: EX31_RS19400 - NADH-quinone oxidoreductase subunit NuoI, at 506,967 to 507,509
_RS19400
EX31_RS19405: EX31_RS19405 - NADH-quinone oxidoreductase subunit NuoH, at 507,524 to 508,501
_RS19405
EX31_RS19410: EX31_RS19410 - NADH-quinone oxidoreductase subunit NuoG, at 508,498 to 511,224
_RS19410
Position (kb) 507
508
509 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 506.745 kb on + strand, within EX31_RS19395 at 506.766 kb on - strand, within EX31_RS19395 at 506.781 kb on + strand, within EX31_RS19395 at 506.782 kb on - strand, within EX31_RS19395 at 506.810 kb on - strand, within EX31_RS19395 at 506.863 kb on + strand, within EX31_RS19395 at 506.863 kb on + strand, within EX31_RS19395 at 506.864 kb on - strand, within EX31_RS19395 at 506.937 kb on - strand at 506.938 kb on + strand at 506.939 kb on - strand at 507.068 kb on - strand, within EX31_RS19400 at 507.116 kb on - strand, within EX31_RS19400 at 507.287 kb on + strand, within EX31_RS19400 at 507.287 kb on + strand, within EX31_RS19400 at 507.306 kb on + strand, within EX31_RS19400 at 507.307 kb on - strand, within EX31_RS19400 at 507.321 kb on - strand, within EX31_RS19400 at 507.413 kb on - strand, within EX31_RS19400 at 507.451 kb on - strand, within EX31_RS19400 at 507.493 kb on - strand at 507.513 kb on + strand at 507.513 kb on + strand at 507.513 kb on + strand at 507.513 kb on + strand at 507.513 kb on + strand at 507.513 kb on + strand at 507.514 kb on - strand at 507.514 kb on - strand at 507.514 kb on - strand at 507.525 kb on + strand at 507.526 kb on - strand at 507.568 kb on + strand at 507.569 kb on - strand at 507.569 kb on - strand at 507.573 kb on - strand at 507.573 kb on - strand at 507.573 kb on - strand at 507.777 kb on + strand, within EX31_RS19405 at 507.777 kb on + strand, within EX31_RS19405 at 507.778 kb on - strand, within EX31_RS19405 at 507.778 kb on - strand, within EX31_RS19405 at 507.778 kb on - strand, within EX31_RS19405 at 508.036 kb on - strand, within EX31_RS19405 at 508.090 kb on + strand, within EX31_RS19405 at 508.157 kb on - strand, within EX31_RS19405 at 508.252 kb on + strand, within EX31_RS19405 at 508.252 kb on + strand, within EX31_RS19405 at 508.481 kb on + strand at 508.482 kb on - strand at 508.489 kb on + strand at 508.722 kb on + strand at 508.723 kb on - strand at 508.723 kb on - strand at 508.723 kb on - strand at 508.781 kb on + strand, within EX31_RS19410 at 508.782 kb on - strand, within EX31_RS19410 at 508.987 kb on - strand, within EX31_RS19410 at 509.088 kb on + strand, within EX31_RS19410 at 509.165 kb on - strand, within EX31_RS19410 at 509.165 kb on - strand, within EX31_RS19410 at 509.309 kb on + strand, within EX31_RS19410 at 509.309 kb on + strand, within EX31_RS19410 at 509.310 kb on - strand, within EX31_RS19410 at 509.310 kb on - strand, within EX31_RS19410 at 509.310 kb on - strand, within EX31_RS19410 at 509.313 kb on - strand, within EX31_RS19410 at 509.424 kb on + strand, within EX31_RS19410 at 509.425 kb on - strand, within EX31_RS19410
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 506,745 + EX31_RS19395 0.63 -0.8 506,766 - EX31_RS19395 0.66 +0.0 506,781 + EX31_RS19395 0.69 +0.0 506,782 - EX31_RS19395 0.69 -1.4 506,810 - EX31_RS19395 0.74 -0.7 506,863 + EX31_RS19395 0.84 -0.0 506,863 + EX31_RS19395 0.84 +1.0 506,864 - EX31_RS19395 0.84 +0.7 506,937 - +3.0 506,938 + -1.1 506,939 - +0.3 507,068 - EX31_RS19400 0.19 +1.0 507,116 - EX31_RS19400 0.27 +0.0 507,287 + EX31_RS19400 0.59 +2.0 507,287 + EX31_RS19400 0.59 +1.0 507,306 + EX31_RS19400 0.62 +1.5 507,307 - EX31_RS19400 0.63 +0.3 507,321 - EX31_RS19400 0.65 -0.1 507,413 - EX31_RS19400 0.82 +0.5 507,451 - EX31_RS19400 0.89 +0.5 507,493 - -1.7 507,513 + +0.0 507,513 + -0.1 507,513 + +1.8 507,513 + -0.5 507,513 + +0.7 507,513 + +0.5 507,514 - +0.5 507,514 - +1.3 507,514 - -0.3 507,525 + +1.5 507,526 - +0.4 507,568 + +1.0 507,569 - +0.0 507,569 - -0.5 507,573 - +0.0 507,573 - -0.7 507,573 - +0.7 507,777 + EX31_RS19405 0.26 +0.0 507,777 + EX31_RS19405 0.26 +0.7 507,778 - EX31_RS19405 0.26 +1.0 507,778 - EX31_RS19405 0.26 -2.4 507,778 - EX31_RS19405 0.26 +0.7 508,036 - EX31_RS19405 0.52 -0.8 508,090 + EX31_RS19405 0.58 +0.8 508,157 - EX31_RS19405 0.65 -1.7 508,252 + EX31_RS19405 0.74 +0.2 508,252 + EX31_RS19405 0.74 +0.0 508,481 + +0.5 508,482 - +1.0 508,489 + -0.9 508,722 + +1.1 508,723 - -1.4 508,723 - +2.0 508,723 - -0.5 508,781 + EX31_RS19410 0.10 -0.1 508,782 - EX31_RS19410 0.10 +2.6 508,987 - EX31_RS19410 0.18 -1.5 509,088 + EX31_RS19410 0.22 +1.0 509,165 - EX31_RS19410 0.24 +1.0 509,165 - EX31_RS19410 0.24 +1.0 509,309 + EX31_RS19410 0.30 +0.0 509,309 + EX31_RS19410 0.30 +1.5 509,310 - EX31_RS19410 0.30 +1.0 509,310 - EX31_RS19410 0.30 -1.5 509,310 - EX31_RS19410 0.30 +0.8 509,313 - EX31_RS19410 0.30 -2.2 509,424 + EX31_RS19410 0.34 +2.0 509,425 - EX31_RS19410 0.34 +1.8
Or see this region's nucleotide sequence