Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18250 and EX31_RS18255 are separated by 104 nucleotides EX31_RS18255 and EX31_RS18260 are separated by 325 nucleotides
EX31_RS18250: EX31_RS18250 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 220,439 to 221,722
_RS18250
EX31_RS18255: EX31_RS18255 - 3-phosphoserine/phosphohydroxythreonine transaminase, at 221,827 to 222,912
_RS18255
EX31_RS18260: EX31_RS18260 - 30S ribosomal protein S12 methylthiotransferase accessory factor YcaO, at 223,238 to 225,001
_RS18260
Position (kb) 221
222
223 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 221.829 kb on - strand at 221.829 kb on - strand at 221.829 kb on - strand at 221.829 kb on - strand at 221.829 kb on - strand at 221.829 kb on - strand at 221.901 kb on - strand at 221.905 kb on - strand at 221.905 kb on - strand at 222.087 kb on - strand, within EX31_RS18255 at 222.166 kb on - strand, within EX31_RS18255 at 222.166 kb on - strand, within EX31_RS18255 at 222.177 kb on - strand, within EX31_RS18255 at 222.208 kb on - strand, within EX31_RS18255 at 222.208 kb on - strand, within EX31_RS18255 at 222.392 kb on + strand, within EX31_RS18255 at 222.401 kb on - strand, within EX31_RS18255 at 222.479 kb on + strand, within EX31_RS18255 at 222.532 kb on - strand, within EX31_RS18255 at 222.544 kb on - strand, within EX31_RS18255 at 222.544 kb on - strand, within EX31_RS18255 at 222.591 kb on + strand, within EX31_RS18255 at 222.787 kb on - strand, within EX31_RS18255 at 222.814 kb on - strand at 222.832 kb on - strand at 222.944 kb on + strand at 222.944 kb on + strand at 222.944 kb on + strand at 222.945 kb on - strand at 222.945 kb on - strand at 222.945 kb on - strand at 222.945 kb on - strand at 222.945 kb on - strand at 222.945 kb on - strand at 222.945 kb on - strand at 222.945 kb on - strand at 222.945 kb on - strand at 222.945 kb on - strand at 222.945 kb on - strand at 222.945 kb on - strand at 222.945 kb on - strand at 222.945 kb on - strand at 222.945 kb on - strand at 222.945 kb on - strand at 222.945 kb on - strand at 222.947 kb on + strand at 222.947 kb on + strand at 222.947 kb on + strand at 222.948 kb on - strand at 222.948 kb on - strand at 222.948 kb on - strand at 222.948 kb on - strand at 222.948 kb on - strand at 222.948 kb on - strand at 222.948 kb on - strand at 222.948 kb on - strand at 222.955 kb on - strand at 222.968 kb on + strand at 222.968 kb on + strand at 223.015 kb on + strand at 223.080 kb on + strand at 223.080 kb on + strand at 223.081 kb on - strand at 223.081 kb on - strand at 223.081 kb on - strand at 223.081 kb on - strand at 223.081 kb on - strand at 223.081 kb on - strand at 223.081 kb on - strand at 223.081 kb on - strand at 223.081 kb on - strand at 223.081 kb on - strand at 223.086 kb on + strand at 223.110 kb on + strand at 223.111 kb on - strand at 223.112 kb on + strand at 223.113 kb on - strand at 223.113 kb on - strand at 223.113 kb on - strand at 223.156 kb on - strand at 223.156 kb on - strand at 223.163 kb on + strand at 223.172 kb on + strand at 223.173 kb on - strand at 223.173 kb on - strand at 223.173 kb on - strand at 223.173 kb on - strand at 223.173 kb on - strand at 223.182 kb on + strand at 223.182 kb on + strand at 223.183 kb on - strand at 223.183 kb on - strand at 223.183 kb on - strand at 223.183 kb on - strand at 223.183 kb on - strand at 223.183 kb on - strand at 223.262 kb on - strand at 223.324 kb on + strand at 223.463 kb on + strand, within EX31_RS18260 at 223.484 kb on - strand, within EX31_RS18260 at 223.487 kb on + strand, within EX31_RS18260 at 223.487 kb on + strand, within EX31_RS18260 at 223.487 kb on + strand, within EX31_RS18260 at 223.487 kb on + strand, within EX31_RS18260 at 223.487 kb on + strand, within EX31_RS18260 at 223.487 kb on + strand, within EX31_RS18260 at 223.488 kb on - strand, within EX31_RS18260 at 223.488 kb on - strand, within EX31_RS18260 at 223.488 kb on - strand, within EX31_RS18260 at 223.488 kb on - strand, within EX31_RS18260 at 223.625 kb on + strand, within EX31_RS18260 at 223.626 kb on - strand, within EX31_RS18260 at 223.626 kb on - strand, within EX31_RS18260 at 223.693 kb on + strand, within EX31_RS18260 at 223.711 kb on + strand, within EX31_RS18260 at 223.712 kb on - strand, within EX31_RS18260 at 223.739 kb on + strand, within EX31_RS18260 at 223.739 kb on + strand, within EX31_RS18260 at 223.739 kb on + strand, within EX31_RS18260 at 223.740 kb on - strand, within EX31_RS18260 at 223.740 kb on - strand, within EX31_RS18260 at 223.741 kb on + strand, within EX31_RS18260 at 223.741 kb on + strand, within EX31_RS18260 at 223.769 kb on + strand, within EX31_RS18260 at 223.847 kb on + strand, within EX31_RS18260 at 223.847 kb on + strand, within EX31_RS18260
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 221,829 - -0.6 221,829 - +1.1 221,829 - -0.5 221,829 - -0.9 221,829 - -0.3 221,829 - +1.7 221,901 - -0.6 221,905 - -0.6 221,905 - -1.8 222,087 - EX31_RS18255 0.24 -2.0 222,166 - EX31_RS18255 0.31 -0.1 222,166 - EX31_RS18255 0.31 +0.1 222,177 - EX31_RS18255 0.32 +0.9 222,208 - EX31_RS18255 0.35 -1.8 222,208 - EX31_RS18255 0.35 -2.5 222,392 + EX31_RS18255 0.52 -0.4 222,401 - EX31_RS18255 0.53 +1.9 222,479 + EX31_RS18255 0.60 +0.3 222,532 - EX31_RS18255 0.65 -0.4 222,544 - EX31_RS18255 0.66 -1.3 222,544 - EX31_RS18255 0.66 -1.8 222,591 + EX31_RS18255 0.70 +2.6 222,787 - EX31_RS18255 0.88 -0.9 222,814 - -0.9 222,832 - -0.3 222,944 + -0.1 222,944 + +0.9 222,944 + -0.3 222,945 - -0.9 222,945 - -1.3 222,945 - -0.6 222,945 - +0.2 222,945 - +1.9 222,945 - +0.5 222,945 - +0.1 222,945 - +1.1 222,945 - +0.5 222,945 - +1.5 222,945 - +1.3 222,945 - -0.1 222,945 - +0.5 222,945 - +0.4 222,945 - +1.1 222,945 - +0.2 222,945 - -0.6 222,947 + +0.4 222,947 + +0.5 222,947 + +0.3 222,948 - -1.0 222,948 - -1.1 222,948 - +0.6 222,948 - +0.4 222,948 - +0.3 222,948 - +1.0 222,948 - +0.2 222,948 - -0.2 222,955 - +0.1 222,968 + -0.4 222,968 + -0.1 223,015 + +0.5 223,080 + +0.8 223,080 + +0.7 223,081 - -0.8 223,081 - +0.5 223,081 - -2.1 223,081 - -0.2 223,081 - +2.9 223,081 - +0.2 223,081 - -0.7 223,081 - +0.2 223,081 - -0.3 223,081 - -0.2 223,086 + -0.2 223,110 + +0.7 223,111 - +0.0 223,112 + +0.9 223,113 - +0.4 223,113 - -1.3 223,113 - -0.4 223,156 - +0.2 223,156 - +0.7 223,163 + +2.4 223,172 + +1.6 223,173 - +0.1 223,173 - +1.9 223,173 - -0.2 223,173 - +0.6 223,173 - +1.0 223,182 + -0.2 223,182 + -0.1 223,183 - -0.4 223,183 - +1.1 223,183 - +0.9 223,183 - -0.1 223,183 - +1.2 223,183 - -0.6 223,262 - +0.2 223,324 + -0.3 223,463 + EX31_RS18260 0.13 +1.3 223,484 - EX31_RS18260 0.14 -0.7 223,487 + EX31_RS18260 0.14 -1.1 223,487 + EX31_RS18260 0.14 +0.5 223,487 + EX31_RS18260 0.14 +1.9 223,487 + EX31_RS18260 0.14 -1.8 223,487 + EX31_RS18260 0.14 +1.1 223,487 + EX31_RS18260 0.14 -0.8 223,488 - EX31_RS18260 0.14 -0.6 223,488 - EX31_RS18260 0.14 +1.4 223,488 - EX31_RS18260 0.14 -0.9 223,488 - EX31_RS18260 0.14 -1.9 223,625 + EX31_RS18260 0.22 +0.6 223,626 - EX31_RS18260 0.22 +0.2 223,626 - EX31_RS18260 0.22 -0.4 223,693 + EX31_RS18260 0.26 -0.7 223,711 + EX31_RS18260 0.27 +1.9 223,712 - EX31_RS18260 0.27 +1.6 223,739 + EX31_RS18260 0.28 -0.1 223,739 + EX31_RS18260 0.28 -0.3 223,739 + EX31_RS18260 0.28 -0.3 223,740 - EX31_RS18260 0.28 +0.7 223,740 - EX31_RS18260 0.28 -0.4 223,741 + EX31_RS18260 0.29 -0.2 223,741 + EX31_RS18260 0.29 -0.5 223,769 + EX31_RS18260 0.30 -1.4 223,847 + EX31_RS18260 0.35 -0.9 223,847 + EX31_RS18260 0.35 -1.2
Or see this region's nucleotide sequence