Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS17240 and EX31_RS17245 are separated by 74 nucleotides EX31_RS17245 and EX31_RS26070 are separated by 3 nucleotides EX31_RS26070 and EX31_RS17250 are separated by 170 nucleotides EX31_RS17250 and EX31_RS17255 overlap by 7 nucleotides EX31_RS17255 and EX31_RS17260 are separated by 10 nucleotides
EX31_RS17240: EX31_RS17240 - hypothetical protein, at 1,363 to 1,578
_RS17240
EX31_RS17245: EX31_RS17245 - hypothetical protein, at 1,653 to 1,916
_RS17245
EX31_RS26070: EX31_RS26070 - hypothetical protein, at 1,920 to 2,093
_RS26070
EX31_RS17250: EX31_RS17250 - DUF1493 family protein, at 2,264 to 2,596
_RS17250
EX31_RS17255: EX31_RS17255 - hypothetical protein, at 2,590 to 3,033
_RS17255
EX31_RS17260: EX31_RS17260 - Hcp family type VI secretion system effector, at 3,044 to 3,529
_RS17260
Position (kb)
2
3 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1.304 kb on + strand at 1.304 kb on + strand at 1.304 kb on + strand at 1.336 kb on + strand at 1.337 kb on - strand at 1.435 kb on + strand, within EX31_RS17240 at 1.435 kb on + strand, within EX31_RS17240 at 1.579 kb on + strand at 1.595 kb on + strand at 1.595 kb on + strand at 1.595 kb on + strand at 1.618 kb on + strand at 1.648 kb on + strand at 1.667 kb on + strand at 1.668 kb on - strand at 1.798 kb on + strand, within EX31_RS17245 at 1.798 kb on + strand, within EX31_RS17245 at 1.828 kb on + strand, within EX31_RS17245 at 1.829 kb on - strand, within EX31_RS17245 at 1.835 kb on + strand, within EX31_RS17245 at 1.835 kb on + strand, within EX31_RS17245 at 1.840 kb on + strand, within EX31_RS17245 at 1.841 kb on - strand, within EX31_RS17245 at 1.866 kb on + strand, within EX31_RS17245 at 1.880 kb on + strand, within EX31_RS17245 at 1.881 kb on - strand, within EX31_RS17245 at 1.928 kb on + strand at 1.929 kb on - strand at 1.938 kb on + strand, within EX31_RS26070 at 1.987 kb on + strand, within EX31_RS26070 at 1.988 kb on - strand, within EX31_RS26070 at 2.003 kb on + strand, within EX31_RS26070 at 2.003 kb on + strand, within EX31_RS26070 at 2.004 kb on - strand, within EX31_RS26070 at 2.060 kb on + strand, within EX31_RS26070 at 2.060 kb on + strand, within EX31_RS26070 at 2.060 kb on + strand, within EX31_RS26070 at 2.060 kb on + strand, within EX31_RS26070 at 2.060 kb on + strand, within EX31_RS26070 at 2.061 kb on - strand, within EX31_RS26070 at 2.061 kb on - strand, within EX31_RS26070 at 2.132 kb on + strand at 2.133 kb on - strand at 2.195 kb on + strand at 2.195 kb on + strand at 2.195 kb on + strand at 2.195 kb on + strand at 2.196 kb on - strand at 2.196 kb on - strand at 2.196 kb on - strand at 2.196 kb on - strand at 2.207 kb on + strand at 2.207 kb on + strand at 2.211 kb on + strand at 2.211 kb on + strand at 2.212 kb on - strand at 2.212 kb on - strand at 2.212 kb on - strand at 2.218 kb on + strand at 2.230 kb on + strand at 2.231 kb on - strand at 2.366 kb on + strand, within EX31_RS17250 at 2.372 kb on - strand, within EX31_RS17250 at 2.382 kb on - strand, within EX31_RS17250 at 2.395 kb on + strand, within EX31_RS17250 at 2.398 kb on - strand, within EX31_RS17250 at 2.439 kb on + strand, within EX31_RS17250 at 2.481 kb on + strand, within EX31_RS17250 at 2.481 kb on + strand, within EX31_RS17250 at 2.481 kb on + strand, within EX31_RS17250 at 2.482 kb on - strand, within EX31_RS17250 at 2.482 kb on - strand, within EX31_RS17250 at 2.530 kb on + strand, within EX31_RS17250 at 2.566 kb on - strand at 2.566 kb on - strand at 2.566 kb on - strand at 2.566 kb on - strand at 2.574 kb on - strand at 2.574 kb on - strand at 2.592 kb on - strand at 2.596 kb on + strand at 2.596 kb on + strand at 2.597 kb on - strand at 2.597 kb on - strand at 2.597 kb on - strand at 2.597 kb on - strand at 2.597 kb on - strand at 2.597 kb on - strand at 2.597 kb on - strand at 2.597 kb on - strand at 2.607 kb on + strand at 2.608 kb on - strand at 2.608 kb on - strand at 2.608 kb on - strand at 2.608 kb on - strand at 2.618 kb on - strand at 2.628 kb on - strand at 2.628 kb on - strand at 2.628 kb on - strand at 2.631 kb on + strand at 2.632 kb on - strand at 2.632 kb on - strand at 2.632 kb on - strand at 2.632 kb on - strand at 2.632 kb on - strand at 2.632 kb on - strand at 2.632 kb on - strand at 2.632 kb on - strand at 2.632 kb on - strand at 2.632 kb on - strand at 2.632 kb on - strand at 2.646 kb on - strand, within EX31_RS17255 at 2.646 kb on - strand, within EX31_RS17255 at 2.646 kb on - strand, within EX31_RS17255 at 2.646 kb on - strand, within EX31_RS17255 at 2.646 kb on - strand, within EX31_RS17255 at 2.656 kb on - strand, within EX31_RS17255 at 2.674 kb on - strand, within EX31_RS17255 at 2.703 kb on + strand, within EX31_RS17255 at 2.704 kb on - strand, within EX31_RS17255 at 2.704 kb on - strand, within EX31_RS17255 at 2.732 kb on + strand, within EX31_RS17255 at 2.732 kb on + strand, within EX31_RS17255 at 2.732 kb on + strand, within EX31_RS17255 at 2.732 kb on + strand, within EX31_RS17255 at 2.732 kb on + strand, within EX31_RS17255 at 2.732 kb on + strand, within EX31_RS17255 at 2.732 kb on + strand, within EX31_RS17255 at 2.732 kb on + strand, within EX31_RS17255 at 2.733 kb on - strand, within EX31_RS17255 at 2.733 kb on - strand, within EX31_RS17255 at 2.733 kb on - strand, within EX31_RS17255 at 2.733 kb on - strand, within EX31_RS17255 at 2.733 kb on - strand, within EX31_RS17255 at 2.733 kb on - strand, within EX31_RS17255 at 2.733 kb on - strand, within EX31_RS17255 at 2.733 kb on - strand, within EX31_RS17255 at 2.733 kb on - strand, within EX31_RS17255 at 2.733 kb on - strand, within EX31_RS17255 at 2.733 kb on - strand, within EX31_RS17255 at 2.800 kb on + strand, within EX31_RS17255 at 2.997 kb on - strand at 3.002 kb on + strand at 3.002 kb on + strand at 3.002 kb on + strand at 3.002 kb on + strand at 3.002 kb on + strand at 3.002 kb on + strand at 3.003 kb on - strand at 3.003 kb on - strand at 3.003 kb on - strand at 3.003 kb on - strand at 3.003 kb on - strand at 3.003 kb on - strand at 3.003 kb on - strand at 3.003 kb on - strand at 3.003 kb on - strand at 3.013 kb on + strand at 3.014 kb on - strand at 3.014 kb on - strand at 3.028 kb on - strand at 3.028 kb on - strand at 3.045 kb on + strand at 3.045 kb on + strand at 3.046 kb on - strand at 3.078 kb on + strand at 3.079 kb on - strand at 3.079 kb on - strand at 3.100 kb on - strand, within EX31_RS17260 at 3.100 kb on - strand, within EX31_RS17260 at 3.100 kb on - strand, within EX31_RS17260 at 3.120 kb on + strand, within EX31_RS17260 at 3.121 kb on - strand, within EX31_RS17260 at 3.121 kb on - strand, within EX31_RS17260 at 3.121 kb on - strand, within EX31_RS17260 at 3.121 kb on - strand, within EX31_RS17260 at 3.150 kb on - strand, within EX31_RS17260 at 3.150 kb on - strand, within EX31_RS17260 at 3.150 kb on - strand, within EX31_RS17260 at 3.150 kb on - strand, within EX31_RS17260 at 3.156 kb on - strand, within EX31_RS17260 at 3.213 kb on - strand, within EX31_RS17260 at 3.213 kb on - strand, within EX31_RS17260 at 3.225 kb on + strand, within EX31_RS17260 at 3.226 kb on - strand, within EX31_RS17260 at 3.226 kb on - strand, within EX31_RS17260 at 3.226 kb on - strand, within EX31_RS17260 at 3.226 kb on - strand, within EX31_RS17260 at 3.226 kb on - strand, within EX31_RS17260 at 3.229 kb on - strand, within EX31_RS17260 at 3.411 kb on + strand, within EX31_RS17260 at 3.424 kb on + strand, within EX31_RS17260 at 3.482 kb on - strand at 3.509 kb on + strand at 3.510 kb on - strand at 3.510 kb on - strand at 3.510 kb on - strand at 3.510 kb on - strand at 3.510 kb on - strand at 3.513 kb on + strand at 3.514 kb on - strand at 3.514 kb on - strand at 3.514 kb on - strand at 3.521 kb on + strand at 3.522 kb on - strand at 3.522 kb on - strand at 3.523 kb on + strand at 3.523 kb on + strand at 3.523 kb on + strand at 3.523 kb on + strand at 3.561 kb on + strand at 3.567 kb on - strand at 3.588 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 1,304 + -0.0 1,304 + +3.1 1,304 + +0.4 1,336 + -0.2 1,337 - +1.0 1,435 + EX31_RS17240 0.33 +1.0 1,435 + EX31_RS17240 0.33 +0.6 1,579 + -0.5 1,595 + +0.8 1,595 + -1.1 1,595 + -0.0 1,618 + -0.1 1,648 + +1.8 1,667 + +0.3 1,668 - +0.2 1,798 + EX31_RS17245 0.55 -1.0 1,798 + EX31_RS17245 0.55 +0.7 1,828 + EX31_RS17245 0.66 -0.3 1,829 - EX31_RS17245 0.67 +0.5 1,835 + EX31_RS17245 0.69 -0.6 1,835 + EX31_RS17245 0.69 +0.1 1,840 + EX31_RS17245 0.71 +0.0 1,841 - EX31_RS17245 0.71 -1.8 1,866 + EX31_RS17245 0.81 +0.8 1,880 + EX31_RS17245 0.86 -1.6 1,881 - EX31_RS17245 0.86 -2.2 1,928 + +0.8 1,929 - +0.8 1,938 + EX31_RS26070 0.10 +0.7 1,987 + EX31_RS26070 0.39 +1.8 1,988 - EX31_RS26070 0.39 -0.4 2,003 + EX31_RS26070 0.48 +1.6 2,003 + EX31_RS26070 0.48 +1.8 2,004 - EX31_RS26070 0.48 -0.7 2,060 + EX31_RS26070 0.80 +1.8 2,060 + EX31_RS26070 0.80 +1.1 2,060 + EX31_RS26070 0.80 +0.2 2,060 + EX31_RS26070 0.80 -0.2 2,060 + EX31_RS26070 0.80 -0.2 2,061 - EX31_RS26070 0.81 +1.8 2,061 - EX31_RS26070 0.81 -0.2 2,132 + +0.0 2,133 - +0.0 2,195 + -0.5 2,195 + -0.9 2,195 + -0.4 2,195 + -0.2 2,196 - +0.2 2,196 - +0.5 2,196 - +0.8 2,196 - +0.3 2,207 + +1.5 2,207 + +0.6 2,211 + -1.2 2,211 + -1.6 2,212 - +1.8 2,212 - +0.1 2,212 - +0.2 2,218 + +0.2 2,230 + +0.1 2,231 - -0.2 2,366 + EX31_RS17250 0.31 -0.2 2,372 - EX31_RS17250 0.32 -1.8 2,382 - EX31_RS17250 0.35 -1.4 2,395 + EX31_RS17250 0.39 +0.6 2,398 - EX31_RS17250 0.40 -0.8 2,439 + EX31_RS17250 0.53 -0.4 2,481 + EX31_RS17250 0.65 -0.1 2,481 + EX31_RS17250 0.65 -1.2 2,481 + EX31_RS17250 0.65 +0.5 2,482 - EX31_RS17250 0.65 -0.5 2,482 - EX31_RS17250 0.65 +0.9 2,530 + EX31_RS17250 0.80 -0.4 2,566 - -0.6 2,566 - +0.5 2,566 - +2.0 2,566 - -1.1 2,574 - +0.3 2,574 - +1.4 2,592 - -0.1 2,596 + +1.6 2,596 + +0.2 2,597 - -0.3 2,597 - +0.4 2,597 - +0.4 2,597 - -1.2 2,597 - -0.5 2,597 - +0.8 2,597 - +0.7 2,597 - +0.9 2,607 + -0.8 2,608 - +1.0 2,608 - -0.6 2,608 - +0.4 2,608 - +0.4 2,618 - +1.8 2,628 - +0.9 2,628 - +0.3 2,628 - +1.6 2,631 + +1.2 2,632 - +3.0 2,632 - -0.4 2,632 - +0.8 2,632 - +1.0 2,632 - -1.2 2,632 - +0.4 2,632 - +1.1 2,632 - -0.2 2,632 - -0.8 2,632 - +0.6 2,632 - +1.5 2,646 - EX31_RS17255 0.13 -0.6 2,646 - EX31_RS17255 0.13 -0.0 2,646 - EX31_RS17255 0.13 +2.1 2,646 - EX31_RS17255 0.13 +2.0 2,646 - EX31_RS17255 0.13 -0.9 2,656 - EX31_RS17255 0.15 +0.2 2,674 - EX31_RS17255 0.19 +0.8 2,703 + EX31_RS17255 0.25 +2.1 2,704 - EX31_RS17255 0.26 +0.6 2,704 - EX31_RS17255 0.26 -1.6 2,732 + EX31_RS17255 0.32 -2.8 2,732 + EX31_RS17255 0.32 -1.0 2,732 + EX31_RS17255 0.32 +0.6 2,732 + EX31_RS17255 0.32 -1.2 2,732 + EX31_RS17255 0.32 +0.5 2,732 + EX31_RS17255 0.32 -2.1 2,732 + EX31_RS17255 0.32 -1.0 2,732 + EX31_RS17255 0.32 +0.9 2,733 - EX31_RS17255 0.32 -1.5 2,733 - EX31_RS17255 0.32 +0.9 2,733 - EX31_RS17255 0.32 +0.4 2,733 - EX31_RS17255 0.32 -0.6 2,733 - EX31_RS17255 0.32 +0.6 2,733 - EX31_RS17255 0.32 +0.4 2,733 - EX31_RS17255 0.32 +1.8 2,733 - EX31_RS17255 0.32 +0.2 2,733 - EX31_RS17255 0.32 +0.5 2,733 - EX31_RS17255 0.32 -1.4 2,733 - EX31_RS17255 0.32 +1.2 2,800 + EX31_RS17255 0.47 +0.4 2,997 - -0.9 3,002 + -1.0 3,002 + +1.1 3,002 + +0.2 3,002 + -0.3 3,002 + +0.8 3,002 + +0.5 3,003 - +1.2 3,003 - -1.5 3,003 - -0.9 3,003 - +0.7 3,003 - -0.6 3,003 - -0.1 3,003 - -1.4 3,003 - +0.5 3,003 - -0.2 3,013 + -0.4 3,014 - -0.2 3,014 - +3.2 3,028 - +1.0 3,028 - +0.4 3,045 + +1.2 3,045 + +0.2 3,046 - -1.0 3,078 + +0.9 3,079 - +0.2 3,079 - +2.8 3,100 - EX31_RS17260 0.12 +0.8 3,100 - EX31_RS17260 0.12 +0.7 3,100 - EX31_RS17260 0.12 +0.4 3,120 + EX31_RS17260 0.16 +0.9 3,121 - EX31_RS17260 0.16 -0.2 3,121 - EX31_RS17260 0.16 -1.0 3,121 - EX31_RS17260 0.16 +0.3 3,121 - EX31_RS17260 0.16 -0.8 3,150 - EX31_RS17260 0.22 -0.2 3,150 - EX31_RS17260 0.22 +0.2 3,150 - EX31_RS17260 0.22 +0.8 3,150 - EX31_RS17260 0.22 +1.2 3,156 - EX31_RS17260 0.23 +1.8 3,213 - EX31_RS17260 0.35 -0.0 3,213 - EX31_RS17260 0.35 +0.4 3,225 + EX31_RS17260 0.37 -0.4 3,226 - EX31_RS17260 0.37 +1.4 3,226 - EX31_RS17260 0.37 +0.5 3,226 - EX31_RS17260 0.37 +1.1 3,226 - EX31_RS17260 0.37 -1.6 3,226 - EX31_RS17260 0.37 +1.1 3,229 - EX31_RS17260 0.38 +0.7 3,411 + EX31_RS17260 0.76 +0.0 3,424 + EX31_RS17260 0.78 -1.8 3,482 - -1.6 3,509 + +0.3 3,510 - -2.0 3,510 - +0.2 3,510 - -0.4 3,510 - +1.0 3,510 - -0.2 3,513 + +2.3 3,514 - +0.2 3,514 - +0.5 3,514 - -0.4 3,521 + -0.1 3,522 - +1.1 3,522 - -0.2 3,523 + -0.4 3,523 + +0.1 3,523 + +1.2 3,523 + +1.8 3,561 + +0.4 3,567 - +0.7 3,588 - +0.8
Or see this region's nucleotide sequence