Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS15400 and EX31_RS15405 overlap by 1 nucleotides EX31_RS15405 and EX31_RS15410 are separated by 106 nucleotides
EX31_RS15400: EX31_RS15400 - nitrogenase cofactor biosynthesis protein NifB, at 31,247 to 32,641
_RS15400
EX31_RS15405: EX31_RS15405 - nitrogen fixation protein NifQ, at 32,641 to 33,156
_RS15405
EX31_RS15410: EX31_RS15410 - IS4 family transposase, at 33,263 to 34,585
_RS15410
Position (kb) 32
33
34 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 31.754 kb on + strand, within EX31_RS15400 at 31.769 kb on + strand, within EX31_RS15400 at 31.784 kb on + strand, within EX31_RS15400 at 31.785 kb on - strand, within EX31_RS15400 at 31.785 kb on - strand, within EX31_RS15400 at 31.787 kb on - strand, within EX31_RS15400 at 31.787 kb on - strand, within EX31_RS15400 at 31.809 kb on + strand, within EX31_RS15400 at 31.809 kb on + strand, within EX31_RS15400 at 31.809 kb on + strand, within EX31_RS15400 at 31.860 kb on + strand, within EX31_RS15400 at 31.860 kb on + strand, within EX31_RS15400 at 31.860 kb on + strand, within EX31_RS15400 at 31.860 kb on + strand, within EX31_RS15400 at 31.909 kb on + strand, within EX31_RS15400 at 31.909 kb on + strand, within EX31_RS15400 at 31.909 kb on + strand, within EX31_RS15400 at 31.909 kb on + strand, within EX31_RS15400 at 31.909 kb on + strand, within EX31_RS15400 at 31.909 kb on + strand, within EX31_RS15400 at 31.909 kb on + strand, within EX31_RS15400 at 31.909 kb on + strand, within EX31_RS15400 at 31.909 kb on + strand, within EX31_RS15400 at 31.909 kb on + strand, within EX31_RS15400 at 31.909 kb on + strand, within EX31_RS15400 at 31.909 kb on + strand, within EX31_RS15400 at 31.909 kb on + strand, within EX31_RS15400 at 31.909 kb on + strand, within EX31_RS15400 at 31.910 kb on - strand, within EX31_RS15400 at 31.910 kb on - strand, within EX31_RS15400 at 31.910 kb on - strand, within EX31_RS15400 at 31.910 kb on - strand, within EX31_RS15400 at 31.910 kb on - strand, within EX31_RS15400 at 31.910 kb on - strand, within EX31_RS15400 at 31.910 kb on - strand, within EX31_RS15400 at 31.910 kb on - strand, within EX31_RS15400 at 31.910 kb on - strand, within EX31_RS15400 at 31.910 kb on - strand, within EX31_RS15400 at 31.957 kb on + strand, within EX31_RS15400 at 32.066 kb on - strand, within EX31_RS15400 at 32.183 kb on + strand, within EX31_RS15400 at 32.224 kb on + strand, within EX31_RS15400 at 32.224 kb on + strand, within EX31_RS15400 at 32.224 kb on + strand, within EX31_RS15400 at 32.224 kb on + strand, within EX31_RS15400 at 32.224 kb on + strand, within EX31_RS15400 at 32.224 kb on + strand, within EX31_RS15400 at 32.224 kb on + strand, within EX31_RS15400 at 32.224 kb on + strand, within EX31_RS15400 at 32.224 kb on + strand, within EX31_RS15400 at 32.224 kb on + strand, within EX31_RS15400 at 32.224 kb on + strand, within EX31_RS15400 at 32.224 kb on + strand, within EX31_RS15400 at 32.225 kb on - strand, within EX31_RS15400 at 32.225 kb on - strand, within EX31_RS15400 at 32.225 kb on - strand, within EX31_RS15400 at 32.225 kb on - strand, within EX31_RS15400 at 32.225 kb on - strand, within EX31_RS15400 at 32.225 kb on - strand, within EX31_RS15400 at 32.229 kb on + strand, within EX31_RS15400 at 32.229 kb on + strand, within EX31_RS15400 at 32.229 kb on + strand, within EX31_RS15400 at 32.229 kb on + strand, within EX31_RS15400 at 32.230 kb on - strand, within EX31_RS15400 at 32.230 kb on - strand, within EX31_RS15400 at 32.329 kb on + strand, within EX31_RS15400 at 32.329 kb on + strand, within EX31_RS15400 at 32.329 kb on + strand, within EX31_RS15400 at 32.330 kb on - strand, within EX31_RS15400 at 32.330 kb on - strand, within EX31_RS15400 at 32.330 kb on - strand, within EX31_RS15400 at 32.336 kb on + strand, within EX31_RS15400 at 32.336 kb on + strand, within EX31_RS15400 at 32.336 kb on + strand, within EX31_RS15400 at 32.337 kb on - strand, within EX31_RS15400 at 32.337 kb on - strand, within EX31_RS15400 at 32.380 kb on + strand, within EX31_RS15400 at 32.390 kb on + strand, within EX31_RS15400 at 32.391 kb on - strand, within EX31_RS15400 at 32.391 kb on - strand, within EX31_RS15400 at 32.478 kb on + strand, within EX31_RS15400 at 32.565 kb on + strand at 32.640 kb on - strand at 32.748 kb on - strand, within EX31_RS15405 at 32.748 kb on - strand, within EX31_RS15405 at 32.789 kb on + strand, within EX31_RS15405 at 32.789 kb on + strand, within EX31_RS15405 at 32.790 kb on - strand, within EX31_RS15405 at 32.831 kb on + strand, within EX31_RS15405 at 32.832 kb on - strand, within EX31_RS15405 at 32.832 kb on - strand, within EX31_RS15405 at 33.033 kb on + strand, within EX31_RS15405 at 33.055 kb on + strand, within EX31_RS15405 at 33.112 kb on - strand at 33.155 kb on - strand at 33.179 kb on + strand at 33.179 kb on + strand at 33.180 kb on - strand at 33.180 kb on - strand at 33.180 kb on - strand at 33.180 kb on - strand at 33.203 kb on + strand at 33.203 kb on + strand at 33.203 kb on + strand at 33.203 kb on + strand at 33.203 kb on + strand at 33.203 kb on + strand at 33.203 kb on + strand at 33.203 kb on + strand at 33.203 kb on + strand at 33.203 kb on + strand at 33.203 kb on + strand at 33.204 kb on - strand at 33.204 kb on - strand at 33.204 kb on - strand at 33.204 kb on - strand at 33.204 kb on - strand at 33.204 kb on - strand at 33.204 kb on - strand at 33.204 kb on - strand at 33.204 kb on - strand at 33.204 kb on - strand at 33.204 kb on - strand at 33.206 kb on + strand at 33.207 kb on - strand at 33.207 kb on - strand at 33.254 kb on + strand at 33.255 kb on - strand at 33.255 kb on - strand at 33.268 kb on + strand at 33.268 kb on + strand at 33.268 kb on + strand at 33.269 kb on - strand at 33.269 kb on - strand at 33.269 kb on - strand at 33.269 kb on - strand at 33.330 kb on + strand at 33.330 kb on + strand at 33.330 kb on + strand at 33.330 kb on + strand at 33.330 kb on + strand at 33.330 kb on + strand at 33.331 kb on - strand at 33.331 kb on - strand at 33.331 kb on - strand at 33.331 kb on - strand at 33.359 kb on + strand at 33.360 kb on - strand at 33.378 kb on + strand at 33.379 kb on - strand at 33.434 kb on + strand, within EX31_RS15410 at 33.443 kb on + strand, within EX31_RS15410 at 33.443 kb on + strand, within EX31_RS15410 at 33.444 kb on - strand, within EX31_RS15410 at 33.444 kb on - strand, within EX31_RS15410 at 33.444 kb on - strand, within EX31_RS15410 at 33.447 kb on - strand, within EX31_RS15410 at 33.490 kb on - strand, within EX31_RS15410 at 33.490 kb on - strand, within EX31_RS15410 at 33.536 kb on - strand, within EX31_RS15410 at 33.536 kb on - strand, within EX31_RS15410 at 33.542 kb on - strand, within EX31_RS15410 at 33.542 kb on - strand, within EX31_RS15410 at 33.544 kb on - strand, within EX31_RS15410 at 33.544 kb on - strand, within EX31_RS15410 at 33.571 kb on - strand, within EX31_RS15410 at 33.571 kb on - strand, within EX31_RS15410 at 33.648 kb on - strand, within EX31_RS15410 at 33.670 kb on + strand, within EX31_RS15410 at 33.670 kb on + strand, within EX31_RS15410 at 33.670 kb on + strand, within EX31_RS15410 at 33.670 kb on + strand, within EX31_RS15410 at 33.671 kb on - strand, within EX31_RS15410 at 33.671 kb on - strand, within EX31_RS15410 at 33.671 kb on - strand, within EX31_RS15410 at 33.671 kb on - strand, within EX31_RS15410 at 33.671 kb on - strand, within EX31_RS15410 at 33.671 kb on - strand, within EX31_RS15410 at 33.671 kb on - strand, within EX31_RS15410 at 33.671 kb on - strand, within EX31_RS15410 at 33.672 kb on + strand, within EX31_RS15410 at 33.673 kb on - strand, within EX31_RS15410 at 33.673 kb on - strand, within EX31_RS15410 at 33.673 kb on - strand, within EX31_RS15410 at 33.728 kb on + strand, within EX31_RS15410 at 33.728 kb on + strand, within EX31_RS15410 at 33.728 kb on + strand, within EX31_RS15410 at 33.729 kb on - strand, within EX31_RS15410 at 33.729 kb on - strand, within EX31_RS15410 at 33.834 kb on - strand, within EX31_RS15410 at 33.859 kb on + strand, within EX31_RS15410 at 33.859 kb on + strand, within EX31_RS15410 at 33.859 kb on + strand, within EX31_RS15410 at 33.859 kb on + strand, within EX31_RS15410 at 33.859 kb on + strand, within EX31_RS15410 at 33.859 kb on + strand, within EX31_RS15410 at 33.860 kb on - strand, within EX31_RS15410 at 33.860 kb on - strand, within EX31_RS15410 at 33.860 kb on - strand, within EX31_RS15410 at 33.860 kb on - strand, within EX31_RS15410 at 33.860 kb on - strand, within EX31_RS15410 at 33.860 kb on - strand, within EX31_RS15410 at 33.860 kb on - strand, within EX31_RS15410 at 33.860 kb on - strand, within EX31_RS15410 at 33.860 kb on - strand, within EX31_RS15410 at 33.925 kb on + strand, within EX31_RS15410 at 33.925 kb on + strand, within EX31_RS15410 at 33.926 kb on - strand, within EX31_RS15410 at 33.926 kb on - strand, within EX31_RS15410 at 33.939 kb on - strand, within EX31_RS15410 at 33.939 kb on - strand, within EX31_RS15410 at 33.939 kb on - strand, within EX31_RS15410 at 33.939 kb on - strand, within EX31_RS15410 at 33.947 kb on + strand, within EX31_RS15410 at 33.948 kb on - strand, within EX31_RS15410 at 33.976 kb on + strand, within EX31_RS15410 at 33.976 kb on + strand, within EX31_RS15410 at 33.977 kb on - strand, within EX31_RS15410 at 33.979 kb on + strand, within EX31_RS15410 at 33.979 kb on + strand, within EX31_RS15410 at 33.979 kb on + strand, within EX31_RS15410 at 33.979 kb on + strand, within EX31_RS15410 at 33.980 kb on - strand, within EX31_RS15410 at 33.980 kb on - strand, within EX31_RS15410 at 33.980 kb on - strand, within EX31_RS15410 at 33.980 kb on - strand, within EX31_RS15410 at 33.980 kb on - strand, within EX31_RS15410 at 33.980 kb on - strand, within EX31_RS15410 at 33.980 kb on - strand, within EX31_RS15410 at 34.038 kb on + strand, within EX31_RS15410 at 34.038 kb on + strand, within EX31_RS15410 at 34.038 kb on + strand, within EX31_RS15410 at 34.039 kb on - strand, within EX31_RS15410
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 31,754 + EX31_RS15400 0.36 +0.8 31,769 + EX31_RS15400 0.37 -0.1 31,784 + EX31_RS15400 0.38 -0.1 31,785 - EX31_RS15400 0.39 +1.5 31,785 - EX31_RS15400 0.39 +1.0 31,787 - EX31_RS15400 0.39 -0.3 31,787 - EX31_RS15400 0.39 -1.2 31,809 + EX31_RS15400 0.40 +0.5 31,809 + EX31_RS15400 0.40 +0.4 31,809 + EX31_RS15400 0.40 -0.9 31,860 + EX31_RS15400 0.44 -1.0 31,860 + EX31_RS15400 0.44 +0.8 31,860 + EX31_RS15400 0.44 +0.6 31,860 + EX31_RS15400 0.44 +3.0 31,909 + EX31_RS15400 0.47 +1.2 31,909 + EX31_RS15400 0.47 +1.0 31,909 + EX31_RS15400 0.47 -0.7 31,909 + EX31_RS15400 0.47 +0.5 31,909 + EX31_RS15400 0.47 -1.2 31,909 + EX31_RS15400 0.47 +0.9 31,909 + EX31_RS15400 0.47 -0.4 31,909 + EX31_RS15400 0.47 -0.3 31,909 + EX31_RS15400 0.47 -0.4 31,909 + EX31_RS15400 0.47 -1.0 31,909 + EX31_RS15400 0.47 +0.2 31,909 + EX31_RS15400 0.47 +0.9 31,909 + EX31_RS15400 0.47 +0.4 31,909 + EX31_RS15400 0.47 +1.0 31,910 - EX31_RS15400 0.48 +2.0 31,910 - EX31_RS15400 0.48 -2.2 31,910 - EX31_RS15400 0.48 -0.3 31,910 - EX31_RS15400 0.48 +1.0 31,910 - EX31_RS15400 0.48 +1.9 31,910 - EX31_RS15400 0.48 -1.7 31,910 - EX31_RS15400 0.48 -0.2 31,910 - EX31_RS15400 0.48 -0.7 31,910 - EX31_RS15400 0.48 +2.0 31,910 - EX31_RS15400 0.48 -0.8 31,957 + EX31_RS15400 0.51 +1.8 32,066 - EX31_RS15400 0.59 +1.5 32,183 + EX31_RS15400 0.67 -0.3 32,224 + EX31_RS15400 0.70 +0.7 32,224 + EX31_RS15400 0.70 +0.2 32,224 + EX31_RS15400 0.70 -0.1 32,224 + EX31_RS15400 0.70 -0.4 32,224 + EX31_RS15400 0.70 +1.0 32,224 + EX31_RS15400 0.70 +0.1 32,224 + EX31_RS15400 0.70 +0.5 32,224 + EX31_RS15400 0.70 +1.6 32,224 + EX31_RS15400 0.70 +0.3 32,224 + EX31_RS15400 0.70 -0.2 32,224 + EX31_RS15400 0.70 +0.7 32,224 + EX31_RS15400 0.70 -0.1 32,225 - EX31_RS15400 0.70 +1.4 32,225 - EX31_RS15400 0.70 -0.3 32,225 - EX31_RS15400 0.70 -0.1 32,225 - EX31_RS15400 0.70 +0.0 32,225 - EX31_RS15400 0.70 -1.3 32,225 - EX31_RS15400 0.70 +0.3 32,229 + EX31_RS15400 0.70 -1.4 32,229 + EX31_RS15400 0.70 -0.4 32,229 + EX31_RS15400 0.70 -0.6 32,229 + EX31_RS15400 0.70 -0.3 32,230 - EX31_RS15400 0.70 -0.8 32,230 - EX31_RS15400 0.70 +0.7 32,329 + EX31_RS15400 0.78 +1.7 32,329 + EX31_RS15400 0.78 +2.0 32,329 + EX31_RS15400 0.78 -2.3 32,330 - EX31_RS15400 0.78 +1.4 32,330 - EX31_RS15400 0.78 +0.5 32,330 - EX31_RS15400 0.78 +0.7 32,336 + EX31_RS15400 0.78 -0.6 32,336 + EX31_RS15400 0.78 +0.5 32,336 + EX31_RS15400 0.78 -0.3 32,337 - EX31_RS15400 0.78 +0.0 32,337 - EX31_RS15400 0.78 -0.3 32,380 + EX31_RS15400 0.81 -0.2 32,390 + EX31_RS15400 0.82 -0.6 32,391 - EX31_RS15400 0.82 +0.8 32,391 - EX31_RS15400 0.82 -0.7 32,478 + EX31_RS15400 0.88 -0.1 32,565 + -0.0 32,640 - +0.3 32,748 - EX31_RS15405 0.21 +0.0 32,748 - EX31_RS15405 0.21 +0.4 32,789 + EX31_RS15405 0.29 +1.2 32,789 + EX31_RS15405 0.29 -0.2 32,790 - EX31_RS15405 0.29 +0.4 32,831 + EX31_RS15405 0.37 -1.2 32,832 - EX31_RS15405 0.37 -0.8 32,832 - EX31_RS15405 0.37 -0.2 33,033 + EX31_RS15405 0.76 -1.0 33,055 + EX31_RS15405 0.80 -1.4 33,112 - +0.3 33,155 - -0.5 33,179 + +0.6 33,179 + -0.4 33,180 - +0.3 33,180 - +1.5 33,180 - -0.6 33,180 - +0.1 33,203 + +1.0 33,203 + -0.6 33,203 + -2.3 33,203 + +0.8 33,203 + +1.6 33,203 + -1.2 33,203 + -1.2 33,203 + -0.6 33,203 + +1.5 33,203 + +0.5 33,203 + +0.1 33,204 - +0.5 33,204 - -0.2 33,204 - -1.7 33,204 - +2.0 33,204 - +1.3 33,204 - +1.7 33,204 - +0.1 33,204 - -1.8 33,204 - -0.3 33,204 - -1.0 33,204 - +2.0 33,206 + +0.3 33,207 - -0.6 33,207 - -1.2 33,254 + +1.7 33,255 - +1.0 33,255 - +0.3 33,268 + +1.0 33,268 + +2.2 33,268 + +0.4 33,269 - -1.4 33,269 - -0.3 33,269 - +0.5 33,269 - -0.6 33,330 + +0.9 33,330 + -2.0 33,330 + +0.8 33,330 + -0.8 33,330 + +0.0 33,330 + -0.2 33,331 - -0.2 33,331 - -1.0 33,331 - +0.1 33,331 - +0.0 33,359 + -0.3 33,360 - +0.4 33,378 + +0.0 33,379 - +0.3 33,434 + EX31_RS15410 0.13 +0.1 33,443 + EX31_RS15410 0.14 +0.0 33,443 + EX31_RS15410 0.14 +4.0 33,444 - EX31_RS15410 0.14 -0.2 33,444 - EX31_RS15410 0.14 -0.9 33,444 - EX31_RS15410 0.14 +1.0 33,447 - EX31_RS15410 0.14 -0.4 33,490 - EX31_RS15410 0.17 +1.3 33,490 - EX31_RS15410 0.17 -0.1 33,536 - EX31_RS15410 0.21 +0.4 33,536 - EX31_RS15410 0.21 -1.7 33,542 - EX31_RS15410 0.21 +1.2 33,542 - EX31_RS15410 0.21 -0.3 33,544 - EX31_RS15410 0.21 +1.3 33,544 - EX31_RS15410 0.21 -0.3 33,571 - EX31_RS15410 0.23 +0.2 33,571 - EX31_RS15410 0.23 +0.6 33,648 - EX31_RS15410 0.29 +1.0 33,670 + EX31_RS15410 0.31 +2.7 33,670 + EX31_RS15410 0.31 +4.2 33,670 + EX31_RS15410 0.31 +1.5 33,670 + EX31_RS15410 0.31 -0.8 33,671 - EX31_RS15410 0.31 +0.7 33,671 - EX31_RS15410 0.31 -0.1 33,671 - EX31_RS15410 0.31 +0.0 33,671 - EX31_RS15410 0.31 +1.1 33,671 - EX31_RS15410 0.31 +0.8 33,671 - EX31_RS15410 0.31 +0.5 33,671 - EX31_RS15410 0.31 +0.2 33,671 - EX31_RS15410 0.31 +0.1 33,672 + EX31_RS15410 0.31 +3.0 33,673 - EX31_RS15410 0.31 -0.5 33,673 - EX31_RS15410 0.31 -0.1 33,673 - EX31_RS15410 0.31 +2.4 33,728 + EX31_RS15410 0.35 +1.4 33,728 + EX31_RS15410 0.35 +1.8 33,728 + EX31_RS15410 0.35 -0.3 33,729 - EX31_RS15410 0.35 -0.7 33,729 - EX31_RS15410 0.35 +0.2 33,834 - EX31_RS15410 0.43 -0.3 33,859 + EX31_RS15410 0.45 -1.2 33,859 + EX31_RS15410 0.45 +1.0 33,859 + EX31_RS15410 0.45 +0.0 33,859 + EX31_RS15410 0.45 -0.5 33,859 + EX31_RS15410 0.45 +0.0 33,859 + EX31_RS15410 0.45 +1.0 33,860 - EX31_RS15410 0.45 -0.7 33,860 - EX31_RS15410 0.45 +0.0 33,860 - EX31_RS15410 0.45 +0.0 33,860 - EX31_RS15410 0.45 +0.9 33,860 - EX31_RS15410 0.45 +0.5 33,860 - EX31_RS15410 0.45 +0.5 33,860 - EX31_RS15410 0.45 +0.8 33,860 - EX31_RS15410 0.45 -0.2 33,860 - EX31_RS15410 0.45 -0.2 33,925 + EX31_RS15410 0.50 -0.3 33,925 + EX31_RS15410 0.50 -0.9 33,926 - EX31_RS15410 0.50 -3.6 33,926 - EX31_RS15410 0.50 +1.0 33,939 - EX31_RS15410 0.51 +0.9 33,939 - EX31_RS15410 0.51 +0.4 33,939 - EX31_RS15410 0.51 -0.5 33,939 - EX31_RS15410 0.51 -0.2 33,947 + EX31_RS15410 0.52 +0.5 33,948 - EX31_RS15410 0.52 -0.3 33,976 + EX31_RS15410 0.54 +0.1 33,976 + EX31_RS15410 0.54 +2.1 33,977 - EX31_RS15410 0.54 +0.3 33,979 + EX31_RS15410 0.54 -2.2 33,979 + EX31_RS15410 0.54 +1.8 33,979 + EX31_RS15410 0.54 -0.6 33,979 + EX31_RS15410 0.54 -1.0 33,980 - EX31_RS15410 0.54 -0.4 33,980 - EX31_RS15410 0.54 +0.8 33,980 - EX31_RS15410 0.54 +1.3 33,980 - EX31_RS15410 0.54 +0.3 33,980 - EX31_RS15410 0.54 -0.0 33,980 - EX31_RS15410 0.54 +1.6 33,980 - EX31_RS15410 0.54 +0.3 34,038 + EX31_RS15410 0.59 +0.6 34,038 + EX31_RS15410 0.59 +0.5 34,038 + EX31_RS15410 0.59 +0.9 34,039 - EX31_RS15410 0.59 -0.6
Or see this region's nucleotide sequence