Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS15135 and EX31_RS15140 are separated by 13 nucleotides EX31_RS15140 and EX31_RS15145 overlap by 4 nucleotides
EX31_RS15135: EX31_RS15135 - uroporphyrinogen-III C-methyltransferase, at 42,126 to 43,277
_RS15135
EX31_RS15140: EX31_RS15140 - uroporphyrinogen-III synthase, at 43,291 to 44,040
_RS15140
EX31_RS15145: EX31_RS15145 - hydroxymethylbilane synthase, at 44,037 to 44,978
_RS15145
Position (kb) 43
44
45 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 42.300 kb on - strand, within EX31_RS15135 at 42.300 kb on - strand, within EX31_RS15135 at 42.300 kb on - strand, within EX31_RS15135 at 42.300 kb on - strand, within EX31_RS15135 at 42.300 kb on - strand, within EX31_RS15135 at 42.350 kb on - strand, within EX31_RS15135 at 42.407 kb on + strand, within EX31_RS15135 at 42.408 kb on - strand, within EX31_RS15135 at 42.421 kb on + strand, within EX31_RS15135 at 42.421 kb on + strand, within EX31_RS15135 at 42.421 kb on + strand, within EX31_RS15135 at 42.421 kb on + strand, within EX31_RS15135 at 42.421 kb on + strand, within EX31_RS15135 at 42.421 kb on + strand, within EX31_RS15135 at 42.422 kb on - strand, within EX31_RS15135 at 42.422 kb on - strand, within EX31_RS15135 at 42.422 kb on - strand, within EX31_RS15135 at 42.422 kb on - strand, within EX31_RS15135 at 42.461 kb on + strand, within EX31_RS15135 at 42.462 kb on - strand, within EX31_RS15135 at 42.462 kb on - strand, within EX31_RS15135 at 42.495 kb on - strand, within EX31_RS15135 at 42.587 kb on + strand, within EX31_RS15135 at 42.588 kb on - strand, within EX31_RS15135 at 42.588 kb on - strand, within EX31_RS15135 at 42.588 kb on - strand, within EX31_RS15135 at 42.588 kb on - strand, within EX31_RS15135 at 42.611 kb on + strand, within EX31_RS15135 at 42.664 kb on - strand, within EX31_RS15135 at 42.900 kb on + strand, within EX31_RS15135 at 42.900 kb on + strand, within EX31_RS15135 at 42.900 kb on + strand, within EX31_RS15135 at 42.900 kb on + strand, within EX31_RS15135 at 42.900 kb on + strand, within EX31_RS15135 at 42.900 kb on + strand, within EX31_RS15135 at 42.901 kb on - strand, within EX31_RS15135 at 42.901 kb on - strand, within EX31_RS15135 at 42.901 kb on - strand, within EX31_RS15135 at 42.901 kb on - strand, within EX31_RS15135 at 42.901 kb on - strand, within EX31_RS15135 at 42.909 kb on + strand, within EX31_RS15135 at 42.909 kb on + strand, within EX31_RS15135 at 42.909 kb on + strand, within EX31_RS15135 at 42.909 kb on + strand, within EX31_RS15135 at 42.910 kb on - strand, within EX31_RS15135 at 42.910 kb on - strand, within EX31_RS15135 at 43.087 kb on + strand, within EX31_RS15135 at 43.090 kb on + strand, within EX31_RS15135 at 43.091 kb on - strand, within EX31_RS15135 at 43.091 kb on - strand, within EX31_RS15135 at 43.091 kb on - strand, within EX31_RS15135 at 43.091 kb on - strand, within EX31_RS15135 at 43.119 kb on + strand, within EX31_RS15135 at 43.119 kb on + strand, within EX31_RS15135 at 43.120 kb on - strand, within EX31_RS15135 at 43.120 kb on - strand, within EX31_RS15135 at 43.120 kb on - strand, within EX31_RS15135 at 43.120 kb on - strand, within EX31_RS15135 at 43.120 kb on - strand, within EX31_RS15135 at 43.120 kb on - strand, within EX31_RS15135 at 43.128 kb on + strand, within EX31_RS15135 at 43.128 kb on + strand, within EX31_RS15135 at 43.293 kb on - strand at 43.300 kb on + strand at 43.300 kb on + strand at 43.301 kb on - strand at 43.301 kb on - strand at 43.301 kb on - strand at 43.301 kb on - strand at 43.301 kb on - strand at 43.306 kb on + strand at 43.306 kb on + strand at 43.306 kb on + strand at 43.306 kb on + strand at 43.306 kb on + strand at 43.306 kb on + strand at 43.306 kb on + strand at 43.306 kb on + strand at 43.307 kb on - strand at 43.307 kb on - strand at 43.307 kb on - strand at 43.307 kb on - strand at 43.307 kb on - strand at 43.307 kb on - strand at 43.307 kb on - strand at 43.307 kb on - strand at 43.307 kb on - strand at 43.307 kb on - strand at 44.060 kb on - strand at 44.060 kb on - strand at 44.993 kb on - strand at 45.014 kb on - strand at 45.025 kb on + strand at 45.025 kb on + strand at 45.026 kb on - strand at 45.026 kb on - strand at 45.026 kb on - strand at 45.026 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 42,300 - EX31_RS15135 0.15 +0.1 42,300 - EX31_RS15135 0.15 -0.6 42,300 - EX31_RS15135 0.15 +2.7 42,300 - EX31_RS15135 0.15 -0.1 42,300 - EX31_RS15135 0.15 +1.7 42,350 - EX31_RS15135 0.19 +0.5 42,407 + EX31_RS15135 0.24 +0.1 42,408 - EX31_RS15135 0.24 -1.4 42,421 + EX31_RS15135 0.26 -1.2 42,421 + EX31_RS15135 0.26 +0.4 42,421 + EX31_RS15135 0.26 -2.0 42,421 + EX31_RS15135 0.26 -0.5 42,421 + EX31_RS15135 0.26 -1.3 42,421 + EX31_RS15135 0.26 +0.5 42,422 - EX31_RS15135 0.26 -0.4 42,422 - EX31_RS15135 0.26 -0.3 42,422 - EX31_RS15135 0.26 -0.2 42,422 - EX31_RS15135 0.26 -0.3 42,461 + EX31_RS15135 0.29 -0.8 42,462 - EX31_RS15135 0.29 +0.4 42,462 - EX31_RS15135 0.29 +0.0 42,495 - EX31_RS15135 0.32 +1.2 42,587 + EX31_RS15135 0.40 -0.2 42,588 - EX31_RS15135 0.40 -0.0 42,588 - EX31_RS15135 0.40 -0.1 42,588 - EX31_RS15135 0.40 +1.7 42,588 - EX31_RS15135 0.40 +0.0 42,611 + EX31_RS15135 0.42 -0.9 42,664 - EX31_RS15135 0.47 +0.4 42,900 + EX31_RS15135 0.67 +0.7 42,900 + EX31_RS15135 0.67 -0.2 42,900 + EX31_RS15135 0.67 +1.3 42,900 + EX31_RS15135 0.67 +0.6 42,900 + EX31_RS15135 0.67 -0.3 42,900 + EX31_RS15135 0.67 -0.3 42,901 - EX31_RS15135 0.67 -0.3 42,901 - EX31_RS15135 0.67 -0.3 42,901 - EX31_RS15135 0.67 +0.3 42,901 - EX31_RS15135 0.67 -0.2 42,901 - EX31_RS15135 0.67 +0.1 42,909 + EX31_RS15135 0.68 +0.0 42,909 + EX31_RS15135 0.68 -0.4 42,909 + EX31_RS15135 0.68 +1.0 42,909 + EX31_RS15135 0.68 +0.7 42,910 - EX31_RS15135 0.68 -0.9 42,910 - EX31_RS15135 0.68 -0.6 43,087 + EX31_RS15135 0.83 -0.7 43,090 + EX31_RS15135 0.84 -0.5 43,091 - EX31_RS15135 0.84 +0.2 43,091 - EX31_RS15135 0.84 +0.1 43,091 - EX31_RS15135 0.84 +0.2 43,091 - EX31_RS15135 0.84 +0.3 43,119 + EX31_RS15135 0.86 -0.6 43,119 + EX31_RS15135 0.86 +1.3 43,120 - EX31_RS15135 0.86 +0.2 43,120 - EX31_RS15135 0.86 +1.2 43,120 - EX31_RS15135 0.86 +1.1 43,120 - EX31_RS15135 0.86 +1.3 43,120 - EX31_RS15135 0.86 +0.3 43,120 - EX31_RS15135 0.86 +0.3 43,128 + EX31_RS15135 0.87 +0.6 43,128 + EX31_RS15135 0.87 +0.1 43,293 - +0.5 43,300 + +0.2 43,300 + +0.2 43,301 - -0.5 43,301 - -3.4 43,301 - -0.0 43,301 - +0.1 43,301 - +0.8 43,306 + +0.3 43,306 + +0.7 43,306 + -1.4 43,306 + +1.1 43,306 + -1.3 43,306 + -0.9 43,306 + +2.2 43,306 + -0.5 43,307 - +0.8 43,307 - -0.3 43,307 - -1.0 43,307 - +0.2 43,307 - -0.4 43,307 - -0.4 43,307 - +1.4 43,307 - -0.7 43,307 - -0.5 43,307 - +0.6 44,060 - -0.2 44,060 - +1.1 44,993 - +0.6 45,014 - -0.6 45,025 + +1.0 45,025 + -0.1 45,026 - +0.6 45,026 - +1.0 45,026 - -0.8 45,026 - +0.6
Or see this region's nucleotide sequence