Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12470 and EX31_RS12475 are separated by 87 nucleotides EX31_RS12475 and EX31_RS12480 are separated by 154 nucleotides EX31_RS12480 and EX31_RS12485 are separated by 285 nucleotides
EX31_RS12470: EX31_RS12470 - outer membrane-stress sensor serine endopeptidase DegS, at 159,590 to 160,657
_RS12470
EX31_RS12475: EX31_RS12475 - Do family serine endopeptidase, at 160,745 to 162,112
_RS12475
EX31_RS12480: EX31_RS12480 - DUF1043 family protein, at 162,267 to 162,668
_RS12480
EX31_RS12485: EX31_RS12485 - cell division protein ZapE, at 162,954 to 164,093
_RS12485
Position (kb) 160
161
162
163 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 160.662 kb on - strand at 160.662 kb on - strand at 160.662 kb on - strand at 160.662 kb on - strand at 160.662 kb on - strand at 160.662 kb on - strand at 160.662 kb on - strand at 160.662 kb on - strand at 160.664 kb on - strand at 160.696 kb on - strand at 160.705 kb on + strand at 160.747 kb on - strand at 160.757 kb on + strand at 160.757 kb on + strand at 160.757 kb on + strand at 160.758 kb on - strand at 160.758 kb on - strand at 160.758 kb on - strand at 160.758 kb on - strand at 160.758 kb on - strand at 160.758 kb on - strand at 160.758 kb on - strand at 160.758 kb on - strand at 160.758 kb on - strand at 160.758 kb on - strand at 160.758 kb on - strand at 160.761 kb on + strand at 160.762 kb on - strand at 160.762 kb on - strand at 160.762 kb on - strand at 160.765 kb on + strand at 160.765 kb on + strand at 160.765 kb on + strand at 160.765 kb on + strand at 160.766 kb on - strand at 160.777 kb on + strand at 160.778 kb on - strand at 160.863 kb on - strand at 160.870 kb on + strand at 160.954 kb on + strand, within EX31_RS12475 at 161.033 kb on - strand, within EX31_RS12475 at 161.127 kb on - strand, within EX31_RS12475 at 161.172 kb on - strand, within EX31_RS12475 at 161.172 kb on - strand, within EX31_RS12475 at 161.172 kb on - strand, within EX31_RS12475 at 161.185 kb on + strand, within EX31_RS12475 at 161.185 kb on + strand, within EX31_RS12475 at 161.219 kb on + strand, within EX31_RS12475 at 161.220 kb on - strand, within EX31_RS12475 at 161.220 kb on - strand, within EX31_RS12475 at 161.220 kb on - strand, within EX31_RS12475 at 161.220 kb on - strand, within EX31_RS12475 at 161.426 kb on - strand, within EX31_RS12475 at 161.428 kb on + strand, within EX31_RS12475 at 161.428 kb on + strand, within EX31_RS12475 at 161.429 kb on - strand, within EX31_RS12475 at 161.429 kb on - strand, within EX31_RS12475 at 161.429 kb on - strand, within EX31_RS12475 at 161.503 kb on + strand, within EX31_RS12475 at 161.648 kb on - strand, within EX31_RS12475 at 161.728 kb on + strand, within EX31_RS12475 at 161.728 kb on + strand, within EX31_RS12475 at 161.728 kb on + strand, within EX31_RS12475 at 161.729 kb on - strand, within EX31_RS12475 at 161.729 kb on - strand, within EX31_RS12475 at 161.730 kb on + strand, within EX31_RS12475 at 161.730 kb on + strand, within EX31_RS12475 at 161.730 kb on + strand, within EX31_RS12475 at 161.730 kb on + strand, within EX31_RS12475 at 161.730 kb on + strand, within EX31_RS12475 at 161.730 kb on + strand, within EX31_RS12475 at 161.731 kb on - strand, within EX31_RS12475 at 161.731 kb on - strand, within EX31_RS12475 at 161.731 kb on - strand, within EX31_RS12475 at 161.731 kb on - strand, within EX31_RS12475 at 161.773 kb on + strand, within EX31_RS12475 at 161.773 kb on + strand, within EX31_RS12475 at 161.774 kb on - strand, within EX31_RS12475 at 161.802 kb on + strand, within EX31_RS12475 at 161.802 kb on + strand, within EX31_RS12475 at 161.802 kb on + strand, within EX31_RS12475 at 161.802 kb on + strand, within EX31_RS12475 at 161.802 kb on + strand, within EX31_RS12475 at 161.802 kb on + strand, within EX31_RS12475 at 161.802 kb on + strand, within EX31_RS12475 at 161.803 kb on - strand, within EX31_RS12475 at 161.803 kb on - strand, within EX31_RS12475 at 161.803 kb on - strand, within EX31_RS12475 at 161.803 kb on - strand, within EX31_RS12475 at 161.824 kb on + strand, within EX31_RS12475 at 161.901 kb on + strand, within EX31_RS12475 at 161.970 kb on - strand, within EX31_RS12475 at 161.983 kb on + strand at 161.983 kb on + strand at 161.983 kb on + strand at 161.983 kb on + strand at 161.983 kb on + strand at 161.983 kb on + strand at 161.983 kb on + strand at 161.983 kb on + strand at 161.983 kb on + strand at 161.983 kb on + strand at 161.983 kb on + strand at 161.984 kb on - strand at 161.984 kb on - strand at 161.984 kb on - strand at 161.984 kb on - strand at 161.984 kb on - strand at 161.984 kb on - strand at 161.984 kb on - strand at 161.984 kb on - strand at 161.984 kb on - strand at 162.026 kb on + strand at 162.062 kb on + strand at 162.119 kb on - strand at 162.150 kb on + strand at 162.151 kb on - strand at 162.151 kb on - strand at 162.159 kb on + strand at 162.159 kb on + strand at 162.159 kb on + strand at 162.159 kb on + strand at 162.159 kb on + strand at 162.159 kb on + strand at 162.159 kb on + strand at 162.159 kb on + strand at 162.160 kb on - strand at 162.160 kb on - strand at 162.160 kb on - strand at 162.160 kb on - strand at 162.160 kb on - strand at 162.160 kb on - strand at 162.215 kb on + strand at 162.215 kb on + strand at 162.215 kb on + strand at 162.215 kb on + strand at 162.215 kb on + strand at 162.215 kb on + strand at 162.215 kb on + strand at 162.215 kb on + strand at 162.215 kb on + strand at 162.215 kb on + strand at 162.215 kb on + strand at 162.215 kb on + strand at 162.215 kb on + strand at 162.215 kb on + strand at 162.215 kb on + strand at 162.215 kb on + strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.216 kb on - strand at 162.223 kb on - strand at 162.226 kb on + strand at 162.226 kb on + strand at 162.227 kb on - strand at 162.227 kb on - strand at 162.227 kb on - strand at 162.227 kb on - strand at 162.227 kb on - strand at 162.227 kb on - strand at 162.246 kb on + strand at 162.246 kb on + strand at 162.246 kb on + strand at 162.247 kb on - strand at 162.269 kb on - strand at 162.269 kb on - strand at 162.269 kb on - strand at 162.273 kb on - strand at 162.273 kb on - strand at 162.273 kb on - strand at 162.273 kb on - strand at 162.715 kb on - strand at 162.715 kb on - strand at 162.729 kb on - strand at 162.763 kb on - strand at 162.783 kb on - strand at 162.783 kb on - strand at 162.792 kb on - strand at 162.792 kb on - strand at 162.840 kb on + strand at 162.841 kb on - strand at 162.841 kb on - strand at 162.859 kb on - strand at 163.059 kb on + strand at 163.060 kb on - strand at 163.060 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 160,662 - -0.9 160,662 - +1.7 160,662 - +1.3 160,662 - -0.3 160,662 - +0.2 160,662 - +0.7 160,662 - -0.7 160,662 - -0.4 160,664 - +1.5 160,696 - +0.2 160,705 + -0.0 160,747 - -0.3 160,757 + +0.5 160,757 + -0.1 160,757 + +0.6 160,758 - +0.1 160,758 - -1.6 160,758 - +0.4 160,758 - +0.5 160,758 - -0.4 160,758 - -0.8 160,758 - -1.2 160,758 - +0.2 160,758 - +0.9 160,758 - +0.2 160,758 - +0.3 160,761 + -0.3 160,762 - +0.8 160,762 - +0.7 160,762 - -0.0 160,765 + +0.5 160,765 + -0.9 160,765 + -0.2 160,765 + -0.6 160,766 - +0.8 160,777 + -0.2 160,778 - -1.6 160,863 - -0.2 160,870 + +0.4 160,954 + EX31_RS12475 0.15 +0.4 161,033 - EX31_RS12475 0.21 +0.4 161,127 - EX31_RS12475 0.28 +0.3 161,172 - EX31_RS12475 0.31 -0.6 161,172 - EX31_RS12475 0.31 +0.6 161,172 - EX31_RS12475 0.31 -0.6 161,185 + EX31_RS12475 0.32 -0.6 161,185 + EX31_RS12475 0.32 -0.2 161,219 + EX31_RS12475 0.35 +0.3 161,220 - EX31_RS12475 0.35 -0.5 161,220 - EX31_RS12475 0.35 -1.0 161,220 - EX31_RS12475 0.35 -0.3 161,220 - EX31_RS12475 0.35 +0.5 161,426 - EX31_RS12475 0.50 -0.4 161,428 + EX31_RS12475 0.50 +0.4 161,428 + EX31_RS12475 0.50 +0.3 161,429 - EX31_RS12475 0.50 -0.1 161,429 - EX31_RS12475 0.50 -0.0 161,429 - EX31_RS12475 0.50 -1.2 161,503 + EX31_RS12475 0.55 -0.3 161,648 - EX31_RS12475 0.66 -1.4 161,728 + EX31_RS12475 0.72 -0.3 161,728 + EX31_RS12475 0.72 -0.4 161,728 + EX31_RS12475 0.72 +1.0 161,729 - EX31_RS12475 0.72 -1.5 161,729 - EX31_RS12475 0.72 -0.4 161,730 + EX31_RS12475 0.72 +0.1 161,730 + EX31_RS12475 0.72 +0.8 161,730 + EX31_RS12475 0.72 -0.4 161,730 + EX31_RS12475 0.72 +0.5 161,730 + EX31_RS12475 0.72 -0.4 161,730 + EX31_RS12475 0.72 -0.9 161,731 - EX31_RS12475 0.72 +1.1 161,731 - EX31_RS12475 0.72 +0.3 161,731 - EX31_RS12475 0.72 +0.8 161,731 - EX31_RS12475 0.72 +0.1 161,773 + EX31_RS12475 0.75 +1.0 161,773 + EX31_RS12475 0.75 -0.0 161,774 - EX31_RS12475 0.75 -1.2 161,802 + EX31_RS12475 0.77 -1.2 161,802 + EX31_RS12475 0.77 -0.5 161,802 + EX31_RS12475 0.77 -0.4 161,802 + EX31_RS12475 0.77 -0.1 161,802 + EX31_RS12475 0.77 -0.1 161,802 + EX31_RS12475 0.77 +0.4 161,802 + EX31_RS12475 0.77 -0.6 161,803 - EX31_RS12475 0.77 +0.7 161,803 - EX31_RS12475 0.77 -0.8 161,803 - EX31_RS12475 0.77 -1.0 161,803 - EX31_RS12475 0.77 +0.1 161,824 + EX31_RS12475 0.79 +0.7 161,901 + EX31_RS12475 0.85 -1.2 161,970 - EX31_RS12475 0.90 -0.1 161,983 + -0.4 161,983 + +0.1 161,983 + -1.6 161,983 + -0.1 161,983 + -0.9 161,983 + +0.2 161,983 + -0.2 161,983 + +1.0 161,983 + +0.9 161,983 + +0.5 161,983 + -0.8 161,984 - +0.3 161,984 - -0.2 161,984 - +0.0 161,984 - +0.4 161,984 - -0.1 161,984 - +1.2 161,984 - -1.5 161,984 - -0.7 161,984 - +0.5 162,026 + +1.6 162,062 + +0.2 162,119 - +0.2 162,150 + -1.0 162,151 - +0.3 162,151 - -0.8 162,159 + -0.5 162,159 + -0.9 162,159 + +1.2 162,159 + -0.6 162,159 + +0.5 162,159 + -0.2 162,159 + +0.6 162,159 + +0.6 162,160 - +0.4 162,160 - +0.1 162,160 - +0.3 162,160 - +0.3 162,160 - -0.1 162,160 - +0.7 162,215 + +4.5 162,215 + +0.2 162,215 + -0.6 162,215 + +1.7 162,215 + -0.3 162,215 + +0.3 162,215 + -0.2 162,215 + -0.4 162,215 + +0.2 162,215 + +0.7 162,215 + +0.5 162,215 + -0.4 162,215 + -0.4 162,215 + +0.4 162,215 + +0.1 162,215 + -1.1 162,216 - +0.9 162,216 - +0.1 162,216 - -0.9 162,216 - -0.4 162,216 - +0.2 162,216 - +0.9 162,216 - -0.5 162,216 - +0.4 162,216 - +0.6 162,216 - +0.5 162,216 - -0.1 162,216 - +0.3 162,216 - +0.4 162,216 - +0.4 162,216 - -0.2 162,216 - -0.7 162,216 - -0.5 162,216 - +0.5 162,216 - +0.1 162,216 - +0.2 162,223 - +1.3 162,226 + -0.0 162,226 + +0.9 162,227 - -1.1 162,227 - -0.0 162,227 - -0.2 162,227 - -0.1 162,227 - -0.3 162,227 - +0.0 162,246 + +0.9 162,246 + -0.1 162,246 + -1.3 162,247 - +0.4 162,269 - -1.7 162,269 - -1.0 162,269 - -1.5 162,273 - -0.0 162,273 - +1.2 162,273 - -0.1 162,273 - +0.6 162,715 - +1.0 162,715 - -0.1 162,729 - +0.7 162,763 - -0.5 162,783 - -0.7 162,783 - -0.4 162,792 - +0.7 162,792 - -0.1 162,840 + -0.1 162,841 - +2.9 162,841 - -0.3 162,859 - +0.1 163,059 + -0.0 163,060 - +1.0 163,060 - +0.5
Or see this region's nucleotide sequence