Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12450 and EX31_RS12455 overlap by 4 nucleotides EX31_RS12455 and EX31_RS12460 are separated by 168 nucleotides EX31_RS12460 and EX31_RS12465 are separated by 67 nucleotides EX31_RS12465 and EX31_RS12470 are separated by 76 nucleotides
EX31_RS12450: EX31_RS12450 - phospholipid-binding protein MlaC, at 156,817 to 157,452
_RS12450
EX31_RS12455: EX31_RS12455 - lipid asymmetry maintenance protein MlaB, at 157,449 to 157,763
_RS12455
EX31_RS12460: EX31_RS12460 - BolA family transcriptional regulator, at 157,932 to 158,186
_RS12460
EX31_RS12465: EX31_RS12465 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 158,254 to 159,513
_RS12465
EX31_RS12470: EX31_RS12470 - outer membrane-stress sensor serine endopeptidase DegS, at 159,590 to 160,657
_RS12470
Position (kb) 158
159
160 Strain fitness (log2 ratio) -2
-1
0
1
2 at 157.275 kb on + strand, within EX31_RS12450 at 157.306 kb on + strand, within EX31_RS12450 at 157.306 kb on + strand, within EX31_RS12450 at 157.306 kb on + strand, within EX31_RS12450 at 157.306 kb on + strand, within EX31_RS12450 at 157.306 kb on + strand, within EX31_RS12450 at 157.306 kb on + strand, within EX31_RS12450 at 157.307 kb on - strand, within EX31_RS12450 at 157.307 kb on - strand, within EX31_RS12450 at 157.307 kb on - strand, within EX31_RS12450 at 157.307 kb on - strand, within EX31_RS12450 at 157.307 kb on - strand, within EX31_RS12450 at 157.307 kb on - strand, within EX31_RS12450 at 157.365 kb on + strand, within EX31_RS12450 at 157.365 kb on + strand, within EX31_RS12450 at 157.365 kb on + strand, within EX31_RS12450 at 157.365 kb on + strand, within EX31_RS12450 at 157.366 kb on - strand, within EX31_RS12450 at 157.366 kb on - strand, within EX31_RS12450 at 157.366 kb on - strand, within EX31_RS12450 at 157.507 kb on + strand, within EX31_RS12455 at 157.507 kb on + strand, within EX31_RS12455 at 157.507 kb on + strand, within EX31_RS12455 at 157.508 kb on - strand, within EX31_RS12455 at 157.508 kb on - strand, within EX31_RS12455 at 157.630 kb on + strand, within EX31_RS12455 at 157.659 kb on - strand, within EX31_RS12455 at 157.703 kb on + strand, within EX31_RS12455 at 157.703 kb on + strand, within EX31_RS12455 at 157.703 kb on + strand, within EX31_RS12455 at 157.703 kb on + strand, within EX31_RS12455 at 157.704 kb on - strand, within EX31_RS12455 at 157.704 kb on - strand, within EX31_RS12455 at 157.704 kb on - strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.710 kb on + strand, within EX31_RS12455 at 157.711 kb on - strand, within EX31_RS12455 at 157.711 kb on - strand, within EX31_RS12455 at 157.711 kb on - strand, within EX31_RS12455 at 157.711 kb on - strand, within EX31_RS12455 at 157.711 kb on - strand, within EX31_RS12455 at 157.711 kb on - strand, within EX31_RS12455 at 157.711 kb on - strand, within EX31_RS12455 at 157.711 kb on - strand, within EX31_RS12455 at 157.712 kb on + strand, within EX31_RS12455 at 157.712 kb on + strand, within EX31_RS12455 at 157.713 kb on - strand, within EX31_RS12455 at 157.713 kb on - strand, within EX31_RS12455 at 157.713 kb on - strand, within EX31_RS12455 at 157.727 kb on + strand, within EX31_RS12455 at 157.727 kb on + strand, within EX31_RS12455 at 157.728 kb on - strand, within EX31_RS12455 at 157.761 kb on + strand at 157.761 kb on + strand at 157.761 kb on + strand at 157.761 kb on + strand at 157.761 kb on + strand at 157.762 kb on - strand at 157.765 kb on + strand at 157.765 kb on + strand at 157.765 kb on + strand at 157.765 kb on + strand at 157.765 kb on + strand at 157.765 kb on + strand at 157.765 kb on + strand at 157.766 kb on - strand at 157.766 kb on - strand at 157.766 kb on - strand at 157.766 kb on - strand at 157.766 kb on - strand at 157.766 kb on - strand at 157.767 kb on + strand at 157.767 kb on + strand at 157.767 kb on + strand at 157.767 kb on + strand at 157.767 kb on + strand at 157.767 kb on + strand at 157.767 kb on + strand at 157.767 kb on + strand at 157.768 kb on - strand at 157.768 kb on - strand at 157.768 kb on - strand at 157.768 kb on - strand at 157.768 kb on - strand at 157.768 kb on - strand at 157.787 kb on + strand at 157.805 kb on + strand at 157.805 kb on + strand at 157.805 kb on + strand at 157.805 kb on + strand at 157.805 kb on + strand at 157.805 kb on + strand at 157.805 kb on + strand at 157.805 kb on + strand at 157.805 kb on + strand at 157.805 kb on + strand at 157.806 kb on - strand at 157.806 kb on - strand at 157.806 kb on - strand at 157.806 kb on - strand at 157.806 kb on - strand at 157.806 kb on - strand at 157.806 kb on - strand at 157.889 kb on + strand at 157.889 kb on + strand at 157.889 kb on + strand at 157.905 kb on + strand at 157.923 kb on + strand at 157.923 kb on + strand at 157.931 kb on + strand at 157.949 kb on + strand at 157.994 kb on + strand, within EX31_RS12460 at 157.994 kb on + strand, within EX31_RS12460 at 158.097 kb on + strand, within EX31_RS12460 at 158.097 kb on + strand, within EX31_RS12460 at 158.097 kb on + strand, within EX31_RS12460 at 158.129 kb on + strand, within EX31_RS12460 at 158.129 kb on + strand, within EX31_RS12460 at 158.129 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.139 kb on + strand, within EX31_RS12460 at 158.182 kb on + strand at 159.533 kb on + strand at 159.534 kb on - strand at 159.534 kb on - strand at 159.604 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 157,275 + EX31_RS12450 0.72 -0.2 157,306 + EX31_RS12450 0.77 +0.7 157,306 + EX31_RS12450 0.77 +0.2 157,306 + EX31_RS12450 0.77 +0.8 157,306 + EX31_RS12450 0.77 +0.4 157,306 + EX31_RS12450 0.77 +0.3 157,306 + EX31_RS12450 0.77 +1.1 157,307 - EX31_RS12450 0.77 +1.1 157,307 - EX31_RS12450 0.77 -0.9 157,307 - EX31_RS12450 0.77 -0.9 157,307 - EX31_RS12450 0.77 +0.3 157,307 - EX31_RS12450 0.77 +0.9 157,307 - EX31_RS12450 0.77 -0.1 157,365 + EX31_RS12450 0.86 -0.1 157,365 + EX31_RS12450 0.86 -0.6 157,365 + EX31_RS12450 0.86 +1.7 157,365 + EX31_RS12450 0.86 +0.1 157,366 - EX31_RS12450 0.86 +0.7 157,366 - EX31_RS12450 0.86 +0.5 157,366 - EX31_RS12450 0.86 +1.6 157,507 + EX31_RS12455 0.18 -0.1 157,507 + EX31_RS12455 0.18 -1.1 157,507 + EX31_RS12455 0.18 +1.2 157,508 - EX31_RS12455 0.19 +1.1 157,508 - EX31_RS12455 0.19 +1.1 157,630 + EX31_RS12455 0.57 +0.1 157,659 - EX31_RS12455 0.67 +0.4 157,703 + EX31_RS12455 0.81 +0.4 157,703 + EX31_RS12455 0.81 +0.8 157,703 + EX31_RS12455 0.81 +0.6 157,703 + EX31_RS12455 0.81 -0.3 157,704 - EX31_RS12455 0.81 +0.3 157,704 - EX31_RS12455 0.81 +1.8 157,704 - EX31_RS12455 0.81 -0.3 157,710 + EX31_RS12455 0.83 -0.3 157,710 + EX31_RS12455 0.83 -0.0 157,710 + EX31_RS12455 0.83 +0.1 157,710 + EX31_RS12455 0.83 +0.4 157,710 + EX31_RS12455 0.83 -0.1 157,710 + EX31_RS12455 0.83 +0.7 157,710 + EX31_RS12455 0.83 +1.3 157,710 + EX31_RS12455 0.83 -0.8 157,710 + EX31_RS12455 0.83 +0.5 157,710 + EX31_RS12455 0.83 +0.2 157,710 + EX31_RS12455 0.83 +0.6 157,710 + EX31_RS12455 0.83 +0.0 157,710 + EX31_RS12455 0.83 +0.5 157,710 + EX31_RS12455 0.83 +0.2 157,710 + EX31_RS12455 0.83 +0.4 157,711 - EX31_RS12455 0.83 -1.4 157,711 - EX31_RS12455 0.83 +0.2 157,711 - EX31_RS12455 0.83 -0.1 157,711 - EX31_RS12455 0.83 -1.1 157,711 - EX31_RS12455 0.83 +1.2 157,711 - EX31_RS12455 0.83 +0.1 157,711 - EX31_RS12455 0.83 +0.5 157,711 - EX31_RS12455 0.83 -0.5 157,712 + EX31_RS12455 0.83 -0.3 157,712 + EX31_RS12455 0.83 -0.8 157,713 - EX31_RS12455 0.84 -0.1 157,713 - EX31_RS12455 0.84 +0.7 157,713 - EX31_RS12455 0.84 +0.7 157,727 + EX31_RS12455 0.88 -0.3 157,727 + EX31_RS12455 0.88 +0.4 157,728 - EX31_RS12455 0.89 -0.3 157,761 + +0.4 157,761 + +0.5 157,761 + +0.8 157,761 + -0.4 157,761 + -1.1 157,762 - -0.5 157,765 + +0.8 157,765 + +0.6 157,765 + +0.3 157,765 + -0.2 157,765 + +0.4 157,765 + -0.7 157,765 + +0.2 157,766 - -2.0 157,766 - -0.3 157,766 - +0.4 157,766 - -1.1 157,766 - +0.2 157,766 - +0.5 157,767 + +1.7 157,767 + +0.1 157,767 + +0.2 157,767 + +0.2 157,767 + +0.4 157,767 + +0.3 157,767 + +0.6 157,767 + +1.1 157,768 - +0.7 157,768 - +0.5 157,768 - +1.0 157,768 - -0.2 157,768 - +0.7 157,768 - -0.2 157,787 + +0.2 157,805 + -0.4 157,805 + -0.6 157,805 + +0.0 157,805 + -0.1 157,805 + -0.7 157,805 + +1.1 157,805 + +0.2 157,805 + +0.3 157,805 + -1.9 157,805 + -0.3 157,806 - +1.2 157,806 - +1.1 157,806 - -0.4 157,806 - +0.3 157,806 - -0.5 157,806 - +0.8 157,806 - -1.3 157,889 + +0.2 157,889 + -0.6 157,889 + +0.4 157,905 + -0.1 157,923 + -0.2 157,923 + -1.4 157,931 + -0.9 157,949 + -0.9 157,994 + EX31_RS12460 0.24 -0.6 157,994 + EX31_RS12460 0.24 -1.3 158,097 + EX31_RS12460 0.65 -0.8 158,097 + EX31_RS12460 0.65 -0.1 158,097 + EX31_RS12460 0.65 +0.7 158,129 + EX31_RS12460 0.77 -0.7 158,129 + EX31_RS12460 0.77 +0.7 158,129 + EX31_RS12460 0.77 -0.9 158,139 + EX31_RS12460 0.81 -1.5 158,139 + EX31_RS12460 0.81 -0.8 158,139 + EX31_RS12460 0.81 -1.6 158,139 + EX31_RS12460 0.81 -0.0 158,139 + EX31_RS12460 0.81 -0.0 158,139 + EX31_RS12460 0.81 +1.0 158,139 + EX31_RS12460 0.81 -0.1 158,139 + EX31_RS12460 0.81 -0.1 158,139 + EX31_RS12460 0.81 +0.5 158,139 + EX31_RS12460 0.81 +0.2 158,139 + EX31_RS12460 0.81 -1.1 158,182 + -1.1 159,533 + -0.2 159,534 - -0.8 159,534 - -1.1 159,604 - +0.5
Or see this region's nucleotide sequence