Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12400 and EX31_RS12405 are separated by 101 nucleotides EX31_RS12405 and EX31_RS12410 are separated by 6 nucleotides EX31_RS12410 and EX31_RS12415 overlap by 23 nucleotides EX31_RS12415 and EX31_RS12420 overlap by 4 nucleotides
EX31_RS12400: EX31_RS12400 - RNA polymerase factor sigma-54, at 148,362 to 149,795
_RS12400
EX31_RS12405: EX31_RS12405 - LPS export ABC transporter ATP-binding protein, at 149,897 to 150,622
_RS12405
EX31_RS12410: EX31_RS12410 - lipopolysaccharide ABC transporter substrate-binding protein LptA, at 150,629 to 151,186
_RS12410
EX31_RS12415: EX31_RS12415 - LPS export ABC transporter periplasmic protein LptC, at 151,164 to 151,733
_RS12415
EX31_RS12420: EX31_RS12420 - 3-deoxy-manno-octulosonate-8-phosphatase KdsC, at 151,730 to 152,296
_RS12420
Position (kb) 150
151
152 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 149.713 kb on - strand at 149.740 kb on - strand at 149.740 kb on - strand at 149.740 kb on - strand at 149.740 kb on - strand at 149.795 kb on + strand at 149.795 kb on + strand at 149.796 kb on - strand at 149.796 kb on - strand at 149.796 kb on - strand at 149.796 kb on - strand at 149.796 kb on - strand at 149.796 kb on - strand at 149.796 kb on - strand at 149.796 kb on - strand at 149.796 kb on - strand at 149.796 kb on - strand at 149.796 kb on - strand at 149.796 kb on - strand at 149.796 kb on - strand at 149.806 kb on - strand at 149.863 kb on + strand at 149.863 kb on + strand at 149.863 kb on + strand at 149.863 kb on + strand at 149.863 kb on + strand at 149.863 kb on + strand at 149.863 kb on + strand at 149.863 kb on + strand at 149.864 kb on - strand at 149.864 kb on - strand at 149.864 kb on - strand at 149.864 kb on - strand at 149.864 kb on - strand at 151.257 kb on - strand, within EX31_RS12415 at 151.277 kb on - strand, within EX31_RS12415 at 151.277 kb on - strand, within EX31_RS12415 at 151.277 kb on - strand, within EX31_RS12415 at 151.277 kb on - strand, within EX31_RS12415 at 151.277 kb on - strand, within EX31_RS12415 at 151.277 kb on - strand, within EX31_RS12415 at 151.734 kb on - strand at 151.763 kb on - strand at 151.763 kb on - strand at 151.763 kb on - strand at 151.800 kb on - strand, within EX31_RS12420 at 151.800 kb on - strand, within EX31_RS12420 at 151.829 kb on - strand, within EX31_RS12420 at 151.834 kb on - strand, within EX31_RS12420 at 151.834 kb on - strand, within EX31_RS12420 at 151.834 kb on - strand, within EX31_RS12420 at 151.834 kb on - strand, within EX31_RS12420 at 151.834 kb on - strand, within EX31_RS12420 at 151.834 kb on - strand, within EX31_RS12420 at 151.834 kb on - strand, within EX31_RS12420 at 151.834 kb on - strand, within EX31_RS12420 at 151.834 kb on - strand, within EX31_RS12420 at 151.834 kb on - strand, within EX31_RS12420 at 151.834 kb on - strand, within EX31_RS12420 at 151.834 kb on - strand, within EX31_RS12420 at 151.890 kb on - strand, within EX31_RS12420 at 151.890 kb on - strand, within EX31_RS12420 at 151.890 kb on - strand, within EX31_RS12420 at 151.933 kb on - strand, within EX31_RS12420 at 151.933 kb on - strand, within EX31_RS12420 at 151.933 kb on - strand, within EX31_RS12420 at 151.933 kb on - strand, within EX31_RS12420 at 151.933 kb on - strand, within EX31_RS12420 at 151.933 kb on - strand, within EX31_RS12420 at 151.933 kb on - strand, within EX31_RS12420 at 151.964 kb on - strand, within EX31_RS12420 at 151.964 kb on - strand, within EX31_RS12420 at 151.964 kb on - strand, within EX31_RS12420 at 151.964 kb on - strand, within EX31_RS12420 at 151.964 kb on - strand, within EX31_RS12420 at 151.968 kb on - strand, within EX31_RS12420 at 151.968 kb on - strand, within EX31_RS12420 at 151.968 kb on - strand, within EX31_RS12420 at 151.968 kb on - strand, within EX31_RS12420 at 151.968 kb on - strand, within EX31_RS12420 at 151.968 kb on - strand, within EX31_RS12420 at 151.994 kb on - strand, within EX31_RS12420 at 152.011 kb on - strand, within EX31_RS12420 at 152.011 kb on - strand, within EX31_RS12420 at 152.011 kb on - strand, within EX31_RS12420 at 152.011 kb on - strand, within EX31_RS12420 at 152.011 kb on - strand, within EX31_RS12420 at 152.011 kb on - strand, within EX31_RS12420 at 152.011 kb on - strand, within EX31_RS12420 at 152.011 kb on - strand, within EX31_RS12420 at 152.011 kb on - strand, within EX31_RS12420 at 152.011 kb on - strand, within EX31_RS12420 at 152.022 kb on - strand, within EX31_RS12420 at 152.022 kb on - strand, within EX31_RS12420 at 152.036 kb on - strand, within EX31_RS12420 at 152.075 kb on - strand, within EX31_RS12420 at 152.170 kb on - strand, within EX31_RS12420 at 152.170 kb on - strand, within EX31_RS12420 at 152.170 kb on - strand, within EX31_RS12420 at 152.170 kb on - strand, within EX31_RS12420 at 152.170 kb on - strand, within EX31_RS12420 at 152.170 kb on - strand, within EX31_RS12420 at 152.170 kb on - strand, within EX31_RS12420 at 152.170 kb on - strand, within EX31_RS12420 at 152.170 kb on - strand, within EX31_RS12420 at 152.170 kb on - strand, within EX31_RS12420
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 149,713 - -1.6 149,740 - -3.7 149,740 - -1.3 149,740 - -1.9 149,740 - -2.4 149,795 + -0.1 149,795 + -2.1 149,796 - -0.7 149,796 - -2.3 149,796 - -0.8 149,796 - +1.1 149,796 - -1.1 149,796 - +0.5 149,796 - -1.0 149,796 - -1.1 149,796 - +0.9 149,796 - -1.1 149,796 - -0.4 149,796 - -1.0 149,796 - -0.9 149,806 - -0.9 149,863 + -1.2 149,863 + -1.9 149,863 + -1.4 149,863 + +0.0 149,863 + -0.2 149,863 + -0.6 149,863 + -0.1 149,863 + -1.0 149,864 - +0.7 149,864 - -0.1 149,864 - -0.0 149,864 - +0.5 149,864 - +1.5 151,257 - EX31_RS12415 0.16 -0.1 151,277 - EX31_RS12415 0.20 +1.6 151,277 - EX31_RS12415 0.20 +0.1 151,277 - EX31_RS12415 0.20 -0.3 151,277 - EX31_RS12415 0.20 +0.9 151,277 - EX31_RS12415 0.20 -0.5 151,277 - EX31_RS12415 0.20 -0.6 151,734 - +0.7 151,763 - +0.0 151,763 - -0.3 151,763 - -0.3 151,800 - EX31_RS12420 0.12 -0.7 151,800 - EX31_RS12420 0.12 +0.7 151,829 - EX31_RS12420 0.17 -0.4 151,834 - EX31_RS12420 0.18 -0.7 151,834 - EX31_RS12420 0.18 +0.4 151,834 - EX31_RS12420 0.18 +1.0 151,834 - EX31_RS12420 0.18 -0.5 151,834 - EX31_RS12420 0.18 -0.4 151,834 - EX31_RS12420 0.18 +0.7 151,834 - EX31_RS12420 0.18 -2.6 151,834 - EX31_RS12420 0.18 +1.9 151,834 - EX31_RS12420 0.18 +0.1 151,834 - EX31_RS12420 0.18 +0.4 151,834 - EX31_RS12420 0.18 +1.4 151,834 - EX31_RS12420 0.18 +0.1 151,890 - EX31_RS12420 0.28 +1.5 151,890 - EX31_RS12420 0.28 +0.8 151,890 - EX31_RS12420 0.28 -0.1 151,933 - EX31_RS12420 0.36 -0.1 151,933 - EX31_RS12420 0.36 +0.8 151,933 - EX31_RS12420 0.36 +0.2 151,933 - EX31_RS12420 0.36 +0.5 151,933 - EX31_RS12420 0.36 +0.8 151,933 - EX31_RS12420 0.36 +0.0 151,933 - EX31_RS12420 0.36 +0.8 151,964 - EX31_RS12420 0.41 -1.3 151,964 - EX31_RS12420 0.41 +1.1 151,964 - EX31_RS12420 0.41 +0.6 151,964 - EX31_RS12420 0.41 +0.6 151,964 - EX31_RS12420 0.41 -0.7 151,968 - EX31_RS12420 0.42 +0.8 151,968 - EX31_RS12420 0.42 -0.8 151,968 - EX31_RS12420 0.42 -0.6 151,968 - EX31_RS12420 0.42 +1.3 151,968 - EX31_RS12420 0.42 +0.6 151,968 - EX31_RS12420 0.42 +1.8 151,994 - EX31_RS12420 0.47 -0.6 152,011 - EX31_RS12420 0.50 +0.2 152,011 - EX31_RS12420 0.50 +0.1 152,011 - EX31_RS12420 0.50 -1.5 152,011 - EX31_RS12420 0.50 -0.0 152,011 - EX31_RS12420 0.50 -0.6 152,011 - EX31_RS12420 0.50 +0.7 152,011 - EX31_RS12420 0.50 +0.2 152,011 - EX31_RS12420 0.50 -0.1 152,011 - EX31_RS12420 0.50 +0.9 152,011 - EX31_RS12420 0.50 +0.1 152,022 - EX31_RS12420 0.51 +0.4 152,022 - EX31_RS12420 0.51 -0.6 152,036 - EX31_RS12420 0.54 -0.5 152,075 - EX31_RS12420 0.61 +0.2 152,170 - EX31_RS12420 0.78 -0.5 152,170 - EX31_RS12420 0.78 +0.6 152,170 - EX31_RS12420 0.78 +0.3 152,170 - EX31_RS12420 0.78 +0.2 152,170 - EX31_RS12420 0.78 +1.4 152,170 - EX31_RS12420 0.78 -0.6 152,170 - EX31_RS12420 0.78 +0.9 152,170 - EX31_RS12420 0.78 +0.6 152,170 - EX31_RS12420 0.78 -0.2 152,170 - EX31_RS12420 0.78 -2.2
Or see this region's nucleotide sequence