Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12240 and EX31_RS12245 overlap by 4 nucleotides EX31_RS12245 and EX31_RS12250 are separated by 7 nucleotides EX31_RS12250 and EX31_RS12255 overlap by 11 nucleotides
EX31_RS12240: EX31_RS12240 - rod shape-determining protein MreC, at 115,797 to 116,804
_RS12240
EX31_RS12245: EX31_RS12245 - rod shape-determining protein MreD, at 116,801 to 117,289
_RS12245
EX31_RS12250: EX31_RS12250 - septum formation inhibitor Maf, at 117,297 to 117,890
_RS12250
EX31_RS12255: EX31_RS12255 - ribonuclease G, at 117,880 to 119,349
_RS12255
Position (kb) 116
117
118 Strain fitness (log2 ratio) -2
-1
0
1
2 at 116.599 kb on + strand, within EX31_RS12240 at 116.599 kb on + strand, within EX31_RS12240 at 116.850 kb on + strand, within EX31_RS12245 at 117.296 kb on + strand at 117.296 kb on + strand at 117.296 kb on + strand at 117.296 kb on + strand at 117.296 kb on + strand at 117.296 kb on + strand at 117.297 kb on - strand at 117.297 kb on - strand at 117.297 kb on - strand at 117.309 kb on + strand at 117.309 kb on + strand at 117.309 kb on + strand at 117.310 kb on - strand at 117.310 kb on - strand at 117.343 kb on + strand at 117.343 kb on + strand at 117.426 kb on + strand, within EX31_RS12250 at 117.426 kb on + strand, within EX31_RS12250 at 117.449 kb on + strand, within EX31_RS12250 at 117.506 kb on + strand, within EX31_RS12250 at 117.506 kb on + strand, within EX31_RS12250 at 117.507 kb on - strand, within EX31_RS12250 at 117.569 kb on + strand, within EX31_RS12250 at 117.570 kb on - strand, within EX31_RS12250 at 117.570 kb on - strand, within EX31_RS12250 at 117.570 kb on - strand, within EX31_RS12250 at 117.576 kb on - strand, within EX31_RS12250 at 117.647 kb on + strand, within EX31_RS12250 at 117.647 kb on + strand, within EX31_RS12250 at 117.647 kb on + strand, within EX31_RS12250 at 117.647 kb on + strand, within EX31_RS12250 at 117.647 kb on + strand, within EX31_RS12250 at 117.647 kb on + strand, within EX31_RS12250 at 117.647 kb on + strand, within EX31_RS12250 at 117.647 kb on + strand, within EX31_RS12250 at 117.647 kb on + strand, within EX31_RS12250 at 117.648 kb on - strand, within EX31_RS12250 at 117.683 kb on - strand, within EX31_RS12250 at 117.705 kb on + strand, within EX31_RS12250 at 117.705 kb on + strand, within EX31_RS12250 at 117.705 kb on + strand, within EX31_RS12250 at 117.705 kb on + strand, within EX31_RS12250 at 117.705 kb on + strand, within EX31_RS12250 at 117.705 kb on + strand, within EX31_RS12250 at 117.705 kb on + strand, within EX31_RS12250 at 117.705 kb on + strand, within EX31_RS12250 at 117.705 kb on + strand, within EX31_RS12250 at 117.705 kb on + strand, within EX31_RS12250 at 117.705 kb on + strand, within EX31_RS12250 at 117.706 kb on - strand, within EX31_RS12250 at 117.706 kb on - strand, within EX31_RS12250 at 117.706 kb on - strand, within EX31_RS12250 at 117.706 kb on - strand, within EX31_RS12250 at 117.706 kb on - strand, within EX31_RS12250 at 117.706 kb on - strand, within EX31_RS12250 at 117.706 kb on - strand, within EX31_RS12250 at 117.706 kb on - strand, within EX31_RS12250 at 117.706 kb on - strand, within EX31_RS12250 at 117.706 kb on - strand, within EX31_RS12250 at 117.706 kb on - strand, within EX31_RS12250 at 117.706 kb on - strand, within EX31_RS12250 at 117.706 kb on - strand, within EX31_RS12250 at 117.706 kb on - strand, within EX31_RS12250 at 117.707 kb on + strand, within EX31_RS12250 at 117.707 kb on + strand, within EX31_RS12250 at 117.707 kb on + strand, within EX31_RS12250 at 117.707 kb on + strand, within EX31_RS12250 at 117.707 kb on + strand, within EX31_RS12250 at 117.707 kb on + strand, within EX31_RS12250 at 117.707 kb on + strand, within EX31_RS12250 at 117.708 kb on - strand, within EX31_RS12250 at 117.708 kb on - strand, within EX31_RS12250 at 117.708 kb on - strand, within EX31_RS12250 at 117.745 kb on - strand, within EX31_RS12250 at 117.750 kb on - strand, within EX31_RS12250 at 117.795 kb on + strand, within EX31_RS12250 at 117.795 kb on + strand, within EX31_RS12250 at 117.795 kb on + strand, within EX31_RS12250 at 117.795 kb on + strand, within EX31_RS12250 at 117.795 kb on + strand, within EX31_RS12250 at 117.795 kb on + strand, within EX31_RS12250 at 117.795 kb on + strand, within EX31_RS12250 at 117.795 kb on + strand, within EX31_RS12250 at 117.795 kb on + strand, within EX31_RS12250 at 117.795 kb on + strand, within EX31_RS12250 at 117.795 kb on + strand, within EX31_RS12250 at 117.798 kb on + strand, within EX31_RS12250 at 117.798 kb on + strand, within EX31_RS12250 at 117.869 kb on + strand at 117.869 kb on + strand at 117.870 kb on - strand at 117.870 kb on - strand at 117.893 kb on + strand at 117.893 kb on + strand at 117.894 kb on - strand at 117.931 kb on + strand at 117.932 kb on - strand at 117.999 kb on + strand at 117.999 kb on + strand at 117.999 kb on + strand at 117.999 kb on + strand at 118.003 kb on + strand at 118.003 kb on + strand at 118.003 kb on + strand at 118.004 kb on - strand at 118.004 kb on - strand at 118.006 kb on - strand at 118.014 kb on + strand at 118.015 kb on - strand at 118.082 kb on + strand, within EX31_RS12255 at 118.082 kb on + strand, within EX31_RS12255 at 118.082 kb on + strand, within EX31_RS12255 at 118.083 kb on - strand, within EX31_RS12255 at 118.248 kb on + strand, within EX31_RS12255 at 118.248 kb on + strand, within EX31_RS12255 at 118.249 kb on - strand, within EX31_RS12255 at 118.249 kb on - strand, within EX31_RS12255 at 118.249 kb on - strand, within EX31_RS12255 at 118.249 kb on - strand, within EX31_RS12255 at 118.264 kb on + strand, within EX31_RS12255 at 118.264 kb on + strand, within EX31_RS12255 at 118.265 kb on - strand, within EX31_RS12255
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 116,599 + EX31_RS12240 0.80 +0.9 116,599 + EX31_RS12240 0.80 +0.1 116,850 + EX31_RS12245 0.10 +0.1 117,296 + +0.0 117,296 + -1.2 117,296 + +0.3 117,296 + -0.1 117,296 + +0.7 117,296 + +0.1 117,297 - +2.2 117,297 - +1.5 117,297 - +0.1 117,309 + -0.3 117,309 + +0.0 117,309 + +0.7 117,310 - -1.3 117,310 - -0.6 117,343 + +0.5 117,343 + -0.9 117,426 + EX31_RS12250 0.22 -0.2 117,426 + EX31_RS12250 0.22 -0.5 117,449 + EX31_RS12250 0.26 +0.0 117,506 + EX31_RS12250 0.35 +0.4 117,506 + EX31_RS12250 0.35 -0.4 117,507 - EX31_RS12250 0.35 -0.0 117,569 + EX31_RS12250 0.46 -1.8 117,570 - EX31_RS12250 0.46 +0.8 117,570 - EX31_RS12250 0.46 +1.1 117,570 - EX31_RS12250 0.46 -0.6 117,576 - EX31_RS12250 0.47 -0.4 117,647 + EX31_RS12250 0.59 +1.1 117,647 + EX31_RS12250 0.59 +0.7 117,647 + EX31_RS12250 0.59 -0.2 117,647 + EX31_RS12250 0.59 +1.1 117,647 + EX31_RS12250 0.59 +0.6 117,647 + EX31_RS12250 0.59 -1.0 117,647 + EX31_RS12250 0.59 +0.3 117,647 + EX31_RS12250 0.59 -2.5 117,647 + EX31_RS12250 0.59 +0.5 117,648 - EX31_RS12250 0.59 -0.5 117,683 - EX31_RS12250 0.65 +1.5 117,705 + EX31_RS12250 0.69 -1.5 117,705 + EX31_RS12250 0.69 -0.5 117,705 + EX31_RS12250 0.69 -0.5 117,705 + EX31_RS12250 0.69 +0.4 117,705 + EX31_RS12250 0.69 +1.5 117,705 + EX31_RS12250 0.69 -0.5 117,705 + EX31_RS12250 0.69 -0.6 117,705 + EX31_RS12250 0.69 +1.1 117,705 + EX31_RS12250 0.69 -0.4 117,705 + EX31_RS12250 0.69 +1.1 117,705 + EX31_RS12250 0.69 +0.7 117,706 - EX31_RS12250 0.69 -1.0 117,706 - EX31_RS12250 0.69 -1.4 117,706 - EX31_RS12250 0.69 -1.3 117,706 - EX31_RS12250 0.69 +0.7 117,706 - EX31_RS12250 0.69 -0.5 117,706 - EX31_RS12250 0.69 +0.5 117,706 - EX31_RS12250 0.69 -0.5 117,706 - EX31_RS12250 0.69 -0.1 117,706 - EX31_RS12250 0.69 -1.1 117,706 - EX31_RS12250 0.69 -0.8 117,706 - EX31_RS12250 0.69 -0.2 117,706 - EX31_RS12250 0.69 -0.4 117,706 - EX31_RS12250 0.69 +0.1 117,706 - EX31_RS12250 0.69 -0.3 117,707 + EX31_RS12250 0.69 +0.8 117,707 + EX31_RS12250 0.69 -0.2 117,707 + EX31_RS12250 0.69 -0.3 117,707 + EX31_RS12250 0.69 +0.6 117,707 + EX31_RS12250 0.69 -0.6 117,707 + EX31_RS12250 0.69 +0.9 117,707 + EX31_RS12250 0.69 +1.2 117,708 - EX31_RS12250 0.69 -1.5 117,708 - EX31_RS12250 0.69 -1.2 117,708 - EX31_RS12250 0.69 -1.0 117,745 - EX31_RS12250 0.75 -0.3 117,750 - EX31_RS12250 0.76 -1.2 117,795 + EX31_RS12250 0.84 -0.2 117,795 + EX31_RS12250 0.84 -0.8 117,795 + EX31_RS12250 0.84 +0.4 117,795 + EX31_RS12250 0.84 +0.7 117,795 + EX31_RS12250 0.84 +1.3 117,795 + EX31_RS12250 0.84 -1.9 117,795 + EX31_RS12250 0.84 -0.9 117,795 + EX31_RS12250 0.84 -0.3 117,795 + EX31_RS12250 0.84 -0.2 117,795 + EX31_RS12250 0.84 +0.7 117,795 + EX31_RS12250 0.84 +0.7 117,798 + EX31_RS12250 0.84 +0.1 117,798 + EX31_RS12250 0.84 +0.7 117,869 + +0.5 117,869 + +0.6 117,870 - +1.0 117,870 - -0.7 117,893 + -0.1 117,893 + +1.0 117,894 - +1.5 117,931 + +0.6 117,932 - -0.9 117,999 + +0.1 117,999 + +1.1 117,999 + -0.0 117,999 + -1.2 118,003 + +0.6 118,003 + -0.0 118,003 + +0.5 118,004 - -0.1 118,004 - +1.1 118,006 - -2.2 118,014 + +0.3 118,015 - +0.5 118,082 + EX31_RS12255 0.14 +0.1 118,082 + EX31_RS12255 0.14 -0.1 118,082 + EX31_RS12255 0.14 -0.2 118,083 - EX31_RS12255 0.14 +1.9 118,248 + EX31_RS12255 0.25 +0.3 118,248 + EX31_RS12255 0.25 +1.4 118,249 - EX31_RS12255 0.25 +1.2 118,249 - EX31_RS12255 0.25 -0.7 118,249 - EX31_RS12255 0.25 +0.0 118,249 - EX31_RS12255 0.25 -0.6 118,264 + EX31_RS12255 0.26 +0.1 118,264 + EX31_RS12255 0.26 -0.1 118,265 - EX31_RS12255 0.26 +1.3
Or see this region's nucleotide sequence