Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11670 and EX31_RS11675 are separated by 8 nucleotides EX31_RS11675 and EX31_RS11680 are separated by 87 nucleotides EX31_RS11680 and EX31_RS11685 are separated by 108 nucleotides EX31_RS11685 and EX31_RS11690 are separated by 25 nucleotides
EX31_RS11670: EX31_RS11670 - Holliday junction branch migration DNA helicase RuvB, at 278,120 to 279,127
_RS11670
EX31_RS11675: EX31_RS11675 - Holliday junction branch migration protein RuvA, at 279,136 to 279,750
_RS11675
EX31_RS11680: EX31_RS11680 - crossover junction endodeoxyribonuclease RuvC, at 279,838 to 280,359
_RS11680
EX31_RS11685: EX31_RS11685 - YebC/PmpR family DNA-binding transcriptional regulator, at 280,468 to 281,211
_RS11685
EX31_RS11690: EX31_RS11690 - dihydroneopterin triphosphate diphosphatase, at 281,237 to 281,683
_RS11690
Position (kb) 279
280
281 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 278.875 kb on + strand, within EX31_RS11670 at 278.876 kb on - strand, within EX31_RS11670 at 278.880 kb on - strand, within EX31_RS11670 at 279.163 kb on - strand at 279.322 kb on - strand, within EX31_RS11675 at 279.418 kb on - strand, within EX31_RS11675 at 279.418 kb on - strand, within EX31_RS11675 at 279.418 kb on - strand, within EX31_RS11675 at 279.422 kb on - strand, within EX31_RS11675 at 279.664 kb on - strand, within EX31_RS11675 at 279.736 kb on + strand at 279.737 kb on - strand at 279.787 kb on + strand at 279.787 kb on + strand at 279.787 kb on + strand at 279.788 kb on - strand at 279.788 kb on - strand at 279.788 kb on - strand at 279.788 kb on - strand at 279.788 kb on - strand at 279.788 kb on - strand at 279.788 kb on - strand at 279.788 kb on - strand at 279.788 kb on - strand at 279.790 kb on - strand at 279.790 kb on - strand at 279.790 kb on - strand at 279.790 kb on - strand at 279.790 kb on - strand at 279.840 kb on - strand at 279.866 kb on - strand at 279.866 kb on - strand at 279.988 kb on - strand, within EX31_RS11680 at 280.239 kb on + strand, within EX31_RS11680 at 280.409 kb on - strand at 280.409 kb on - strand at 280.409 kb on - strand at 280.411 kb on - strand at 280.411 kb on - strand at 280.411 kb on - strand at 280.411 kb on - strand at 280.411 kb on - strand at 280.411 kb on - strand at 280.434 kb on - strand at 280.469 kb on + strand at 280.470 kb on - strand at 280.512 kb on - strand at 280.512 kb on - strand at 280.566 kb on + strand, within EX31_RS11685 at 280.567 kb on - strand, within EX31_RS11685 at 280.567 kb on - strand, within EX31_RS11685 at 280.817 kb on + strand, within EX31_RS11685 at 280.818 kb on - strand, within EX31_RS11685 at 280.943 kb on + strand, within EX31_RS11685 at 280.953 kb on - strand, within EX31_RS11685 at 280.953 kb on - strand, within EX31_RS11685 at 280.953 kb on - strand, within EX31_RS11685 at 280.953 kb on - strand, within EX31_RS11685 at 280.953 kb on - strand, within EX31_RS11685 at 281.185 kb on - strand at 281.197 kb on - strand at 281.197 kb on - strand at 281.197 kb on - strand at 281.197 kb on - strand at 281.197 kb on - strand at 281.211 kb on + strand at 281.215 kb on + strand at 281.216 kb on - strand at 281.216 kb on - strand at 281.216 kb on - strand at 281.238 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 278,875 + EX31_RS11670 0.75 -0.3 278,876 - EX31_RS11670 0.75 -0.9 278,880 - EX31_RS11670 0.75 +2.1 279,163 - -1.5 279,322 - EX31_RS11675 0.30 -1.3 279,418 - EX31_RS11675 0.46 -0.4 279,418 - EX31_RS11675 0.46 -1.7 279,418 - EX31_RS11675 0.46 +1.4 279,422 - EX31_RS11675 0.47 +0.1 279,664 - EX31_RS11675 0.86 +0.3 279,736 + +2.3 279,737 - -1.3 279,787 + -0.6 279,787 + +0.3 279,787 + +1.0 279,788 - -0.1 279,788 - +1.7 279,788 - -0.1 279,788 - +0.4 279,788 - -0.3 279,788 - -0.5 279,788 - -1.1 279,788 - -0.8 279,788 - +1.2 279,790 - -2.9 279,790 - +0.5 279,790 - -0.3 279,790 - +0.9 279,790 - +0.1 279,840 - -1.1 279,866 - +0.3 279,866 - +0.7 279,988 - EX31_RS11680 0.29 -2.3 280,239 + EX31_RS11680 0.77 +0.7 280,409 - +0.9 280,409 - +1.3 280,409 - +0.1 280,411 - +1.3 280,411 - -0.2 280,411 - +0.1 280,411 - +0.1 280,411 - -0.1 280,411 - +0.9 280,434 - +0.1 280,469 + +3.3 280,470 - +0.0 280,512 - -1.4 280,512 - +0.3 280,566 + EX31_RS11685 0.13 -1.3 280,567 - EX31_RS11685 0.13 +1.4 280,567 - EX31_RS11685 0.13 +0.4 280,817 + EX31_RS11685 0.47 +0.3 280,818 - EX31_RS11685 0.47 +2.7 280,943 + EX31_RS11685 0.64 +0.3 280,953 - EX31_RS11685 0.65 -0.6 280,953 - EX31_RS11685 0.65 +0.5 280,953 - EX31_RS11685 0.65 -0.3 280,953 - EX31_RS11685 0.65 -0.8 280,953 - EX31_RS11685 0.65 -0.4 281,185 - -0.8 281,197 - -1.4 281,197 - -0.6 281,197 - -1.6 281,197 - -0.1 281,197 - -1.3 281,211 + -0.3 281,215 + +0.1 281,216 - +0.1 281,216 - -0.7 281,216 - -2.0 281,238 + +1.3
Or see this region's nucleotide sequence