Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10170 and EX31_RS10175 overlap by 4 nucleotides EX31_RS10175 and EX31_RS10180 are separated by 16 nucleotides EX31_RS10180 and EX31_RS10185 are separated by 71 nucleotides
EX31_RS10170: EX31_RS10170 - gallate dioxygenase, at 246,659 to 247,921
_RS10170
EX31_RS10175: EX31_RS10175 - amidohydrolase family protein, at 247,918 to 248,751
_RS10175
EX31_RS10180: EX31_RS10180 - type II 3-dehydroquinate dehydratase, at 248,768 to 249,208
_RS10180
EX31_RS10185: EX31_RS10185 - O-antigen ligase family protein, at 249,280 to 250,533
_RS10185
Position (kb) 248
249
250 Strain fitness (log2 ratio) -1
0
1 at 247.821 kb on + strand at 247.821 kb on + strand at 247.821 kb on + strand at 247.822 kb on - strand at 247.822 kb on - strand at 247.825 kb on + strand at 247.825 kb on + strand at 247.825 kb on + strand at 247.825 kb on + strand at 247.825 kb on + strand at 247.825 kb on + strand at 247.825 kb on + strand at 247.825 kb on + strand at 247.826 kb on - strand at 247.826 kb on - strand at 247.826 kb on - strand at 247.826 kb on - strand at 247.829 kb on + strand at 247.830 kb on - strand at 247.830 kb on - strand at 248.002 kb on + strand, within EX31_RS10175 at 248.041 kb on + strand, within EX31_RS10175 at 248.063 kb on - strand, within EX31_RS10175 at 248.063 kb on - strand, within EX31_RS10175 at 248.105 kb on - strand, within EX31_RS10175 at 248.106 kb on + strand, within EX31_RS10175 at 248.106 kb on + strand, within EX31_RS10175 at 248.106 kb on + strand, within EX31_RS10175 at 248.106 kb on + strand, within EX31_RS10175 at 248.106 kb on + strand, within EX31_RS10175 at 248.107 kb on - strand, within EX31_RS10175 at 248.107 kb on - strand, within EX31_RS10175 at 248.107 kb on - strand, within EX31_RS10175 at 248.554 kb on + strand, within EX31_RS10175 at 248.775 kb on - strand at 248.775 kb on - strand at 248.838 kb on - strand, within EX31_RS10180 at 249.138 kb on + strand, within EX31_RS10180 at 249.139 kb on - strand, within EX31_RS10180 at 249.171 kb on - strand at 249.582 kb on + strand, within EX31_RS10185 at 249.621 kb on + strand, within EX31_RS10185 at 249.630 kb on + strand, within EX31_RS10185 at 249.648 kb on - strand, within EX31_RS10185 at 249.650 kb on - strand, within EX31_RS10185 at 249.651 kb on + strand, within EX31_RS10185 at 249.652 kb on - strand, within EX31_RS10185 at 249.652 kb on - strand, within EX31_RS10185 at 249.707 kb on + strand, within EX31_RS10185 at 249.838 kb on - strand, within EX31_RS10185 at 249.844 kb on - strand, within EX31_RS10185 at 250.004 kb on + strand, within EX31_RS10185 at 250.119 kb on + strand, within EX31_RS10185 at 250.124 kb on - strand, within EX31_RS10185
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 247,821 + +1.2 247,821 + -1.2 247,821 + -0.1 247,822 - +1.0 247,822 - +0.6 247,825 + -0.1 247,825 + +0.4 247,825 + -1.2 247,825 + +1.6 247,825 + +0.5 247,825 + -1.0 247,825 + +0.9 247,825 + +1.6 247,826 - +1.3 247,826 - -0.7 247,826 - -0.5 247,826 - +1.0 247,829 + +0.3 247,830 - +0.1 247,830 - +0.3 248,002 + EX31_RS10175 0.10 -0.2 248,041 + EX31_RS10175 0.15 +1.0 248,063 - EX31_RS10175 0.17 +0.9 248,063 - EX31_RS10175 0.17 -0.1 248,105 - EX31_RS10175 0.22 -0.5 248,106 + EX31_RS10175 0.23 +0.2 248,106 + EX31_RS10175 0.23 +0.5 248,106 + EX31_RS10175 0.23 +0.3 248,106 + EX31_RS10175 0.23 -0.6 248,106 + EX31_RS10175 0.23 -0.6 248,107 - EX31_RS10175 0.23 +0.4 248,107 - EX31_RS10175 0.23 +0.4 248,107 - EX31_RS10175 0.23 -0.7 248,554 + EX31_RS10175 0.76 -0.4 248,775 - +0.6 248,775 - +1.6 248,838 - EX31_RS10180 0.16 +0.7 249,138 + EX31_RS10180 0.84 -1.6 249,139 - EX31_RS10180 0.84 -0.4 249,171 - -1.6 249,582 + EX31_RS10185 0.24 +1.1 249,621 + EX31_RS10185 0.27 +1.6 249,630 + EX31_RS10185 0.28 +0.0 249,648 - EX31_RS10185 0.29 +0.1 249,650 - EX31_RS10185 0.30 -1.0 249,651 + EX31_RS10185 0.30 +0.0 249,652 - EX31_RS10185 0.30 +0.9 249,652 - EX31_RS10185 0.30 -0.6 249,707 + EX31_RS10185 0.34 -0.6 249,838 - EX31_RS10185 0.44 -0.3 249,844 - EX31_RS10185 0.45 -0.8 250,004 + EX31_RS10185 0.58 +1.1 250,119 + EX31_RS10185 0.67 +1.0 250,124 - EX31_RS10185 0.67 +0.6
Or see this region's nucleotide sequence