Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09885 and EX31_RS09890 are separated by 191 nucleotides EX31_RS09890 and EX31_RS09895 are separated by 350 nucleotides
EX31_RS09885: EX31_RS09885 - aconitate hydratase AcnA, at 182,827 to 185,499
_RS09885
EX31_RS09890: EX31_RS09890 - HTH-type transcriptional regulator CysB, at 185,691 to 186,665
_RS09890
EX31_RS09895: EX31_RS09895 - type I DNA topoisomerase, at 187,016 to 189,613
_RS09895
Position (kb) 185
186
187 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 184.861 kb on - strand, within EX31_RS09885 at 184.861 kb on - strand, within EX31_RS09885 at 184.922 kb on + strand, within EX31_RS09885 at 184.923 kb on - strand, within EX31_RS09885 at 184.943 kb on + strand, within EX31_RS09885 at 184.943 kb on + strand, within EX31_RS09885 at 184.969 kb on - strand, within EX31_RS09885 at 184.987 kb on + strand, within EX31_RS09885 at 185.153 kb on - strand, within EX31_RS09885 at 185.221 kb on + strand, within EX31_RS09885 at 185.262 kb on - strand at 185.314 kb on + strand at 185.356 kb on + strand at 185.436 kb on - strand at 185.438 kb on + strand at 185.438 kb on + strand at 185.439 kb on - strand at 185.442 kb on - strand at 185.491 kb on - strand at 185.499 kb on + strand at 185.499 kb on + strand at 185.499 kb on + strand at 185.499 kb on + strand at 185.500 kb on - strand at 185.500 kb on - strand at 185.500 kb on - strand at 185.500 kb on - strand at 185.537 kb on - strand at 185.549 kb on + strand at 185.550 kb on - strand at 185.550 kb on - strand at 185.550 kb on - strand at 185.550 kb on - strand at 185.551 kb on + strand at 185.551 kb on + strand at 185.551 kb on + strand at 185.551 kb on + strand at 185.551 kb on + strand at 185.552 kb on - strand at 185.552 kb on - strand at 185.552 kb on - strand at 185.552 kb on - strand at 185.563 kb on + strand at 185.672 kb on - strand at 185.692 kb on + strand at 185.692 kb on + strand at 185.693 kb on - strand at 185.708 kb on + strand at 185.708 kb on + strand at 185.757 kb on - strand at 185.812 kb on + strand, within EX31_RS09890 at 185.813 kb on - strand, within EX31_RS09890 at 185.816 kb on + strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.817 kb on - strand, within EX31_RS09890 at 185.818 kb on + strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.819 kb on - strand, within EX31_RS09890 at 185.867 kb on - strand, within EX31_RS09890 at 185.867 kb on - strand, within EX31_RS09890 at 185.969 kb on - strand, within EX31_RS09890 at 185.969 kb on - strand, within EX31_RS09890 at 185.969 kb on - strand, within EX31_RS09890 at 185.969 kb on - strand, within EX31_RS09890 at 186.004 kb on - strand, within EX31_RS09890 at 186.004 kb on - strand, within EX31_RS09890 at 186.074 kb on + strand, within EX31_RS09890 at 186.074 kb on + strand, within EX31_RS09890 at 186.075 kb on - strand, within EX31_RS09890 at 186.075 kb on - strand, within EX31_RS09890 at 186.075 kb on - strand, within EX31_RS09890 at 186.077 kb on - strand, within EX31_RS09890 at 186.077 kb on - strand, within EX31_RS09890 at 186.077 kb on - strand, within EX31_RS09890 at 186.126 kb on - strand, within EX31_RS09890 at 186.176 kb on - strand, within EX31_RS09890 at 186.203 kb on - strand, within EX31_RS09890 at 186.291 kb on - strand, within EX31_RS09890 at 186.330 kb on - strand, within EX31_RS09890 at 186.363 kb on - strand, within EX31_RS09890 at 186.363 kb on - strand, within EX31_RS09890 at 186.363 kb on - strand, within EX31_RS09890 at 186.380 kb on - strand, within EX31_RS09890 at 186.380 kb on - strand, within EX31_RS09890 at 186.400 kb on + strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.441 kb on - strand, within EX31_RS09890 at 186.448 kb on - strand, within EX31_RS09890 at 186.448 kb on - strand, within EX31_RS09890 at 186.448 kb on - strand, within EX31_RS09890 at 186.448 kb on - strand, within EX31_RS09890 at 186.448 kb on - strand, within EX31_RS09890 at 186.587 kb on - strand at 186.587 kb on - strand at 186.587 kb on - strand at 186.627 kb on - strand at 186.666 kb on - strand at 186.672 kb on + strand at 186.672 kb on + strand at 186.673 kb on - strand at 186.673 kb on - strand at 186.673 kb on - strand at 186.673 kb on - strand at 186.673 kb on - strand at 186.860 kb on + strand at 186.860 kb on + strand at 186.861 kb on - strand at 186.861 kb on - strand at 186.861 kb on - strand at 186.875 kb on + strand at 186.876 kb on - strand at 186.920 kb on + strand at 186.921 kb on - strand at 186.921 kb on - strand at 186.934 kb on + strand at 186.935 kb on - strand at 186.942 kb on - strand at 187.003 kb on - strand at 187.003 kb on - strand at 187.003 kb on - strand at 187.003 kb on - strand at 187.018 kb on - strand at 187.040 kb on - strand at 187.040 kb on - strand at 187.040 kb on - strand at 187.040 kb on - strand at 187.095 kb on - strand at 187.095 kb on - strand at 187.095 kb on - strand at 187.095 kb on - strand at 187.095 kb on - strand at 187.095 kb on - strand at 187.099 kb on - strand at 187.099 kb on - strand at 187.099 kb on - strand at 187.099 kb on - strand at 187.099 kb on - strand at 187.099 kb on - strand at 187.099 kb on - strand at 187.139 kb on - strand at 187.139 kb on - strand at 187.309 kb on - strand, within EX31_RS09895 at 187.432 kb on - strand, within EX31_RS09895 at 187.432 kb on - strand, within EX31_RS09895 at 187.432 kb on - strand, within EX31_RS09895 at 187.432 kb on - strand, within EX31_RS09895 at 187.432 kb on - strand, within EX31_RS09895 at 187.436 kb on - strand, within EX31_RS09895 at 187.436 kb on - strand, within EX31_RS09895 at 187.504 kb on - strand, within EX31_RS09895
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 184,861 - EX31_RS09885 0.76 +0.6 184,861 - EX31_RS09885 0.76 +0.0 184,922 + EX31_RS09885 0.78 -0.9 184,923 - EX31_RS09885 0.78 +0.7 184,943 + EX31_RS09885 0.79 +0.8 184,943 + EX31_RS09885 0.79 +1.0 184,969 - EX31_RS09885 0.80 +1.6 184,987 + EX31_RS09885 0.81 -0.1 185,153 - EX31_RS09885 0.87 -1.4 185,221 + EX31_RS09885 0.90 -0.6 185,262 - +0.6 185,314 + +1.0 185,356 + +0.8 185,436 - -0.6 185,438 + +0.9 185,438 + +1.6 185,439 - -0.4 185,442 - -1.2 185,491 - +0.3 185,499 + -1.0 185,499 + +2.2 185,499 + -0.7 185,499 + +1.0 185,500 - -0.2 185,500 - -0.4 185,500 - -1.0 185,500 - -0.6 185,537 - -1.4 185,549 + +0.3 185,550 - +0.1 185,550 - -0.5 185,550 - +0.2 185,550 - +0.2 185,551 + +0.1 185,551 + -0.8 185,551 + +0.8 185,551 + +3.2 185,551 + +0.9 185,552 - +0.9 185,552 - +1.6 185,552 - +1.1 185,552 - -1.0 185,563 + +1.2 185,672 - +1.6 185,692 + -0.2 185,692 + +0.3 185,693 - +0.0 185,708 + -0.7 185,708 + -0.7 185,757 - -1.9 185,812 + EX31_RS09890 0.12 -0.4 185,813 - EX31_RS09890 0.13 -1.9 185,816 + EX31_RS09890 0.13 -3.0 185,817 - EX31_RS09890 0.13 -3.5 185,817 - EX31_RS09890 0.13 -2.9 185,817 - EX31_RS09890 0.13 -2.9 185,817 - EX31_RS09890 0.13 -1.5 185,817 - EX31_RS09890 0.13 -1.8 185,817 - EX31_RS09890 0.13 -3.5 185,817 - EX31_RS09890 0.13 -1.1 185,817 - EX31_RS09890 0.13 +0.6 185,817 - EX31_RS09890 0.13 -0.2 185,817 - EX31_RS09890 0.13 -2.0 185,817 - EX31_RS09890 0.13 -0.6 185,817 - EX31_RS09890 0.13 -1.6 185,817 - EX31_RS09890 0.13 +0.0 185,817 - EX31_RS09890 0.13 -0.8 185,817 - EX31_RS09890 0.13 -0.7 185,818 + EX31_RS09890 0.13 -1.6 185,819 - EX31_RS09890 0.13 +1.6 185,819 - EX31_RS09890 0.13 -2.9 185,819 - EX31_RS09890 0.13 +1.0 185,819 - EX31_RS09890 0.13 -2.1 185,819 - EX31_RS09890 0.13 -3.8 185,819 - EX31_RS09890 0.13 -4.0 185,819 - EX31_RS09890 0.13 -2.1 185,819 - EX31_RS09890 0.13 -1.3 185,819 - EX31_RS09890 0.13 +0.6 185,819 - EX31_RS09890 0.13 -0.7 185,819 - EX31_RS09890 0.13 -1.7 185,819 - EX31_RS09890 0.13 -3.6 185,819 - EX31_RS09890 0.13 -1.9 185,819 - EX31_RS09890 0.13 -0.9 185,819 - EX31_RS09890 0.13 -0.7 185,819 - EX31_RS09890 0.13 -1.2 185,819 - EX31_RS09890 0.13 -2.5 185,819 - EX31_RS09890 0.13 -2.5 185,819 - EX31_RS09890 0.13 -0.5 185,819 - EX31_RS09890 0.13 -1.6 185,819 - EX31_RS09890 0.13 -1.6 185,867 - EX31_RS09890 0.18 -0.5 185,867 - EX31_RS09890 0.18 -0.6 185,969 - EX31_RS09890 0.29 -2.2 185,969 - EX31_RS09890 0.29 -0.4 185,969 - EX31_RS09890 0.29 -1.0 185,969 - EX31_RS09890 0.29 -2.1 186,004 - EX31_RS09890 0.32 -2.1 186,004 - EX31_RS09890 0.32 -2.9 186,074 + EX31_RS09890 0.39 -0.7 186,074 + EX31_RS09890 0.39 +0.0 186,075 - EX31_RS09890 0.39 -1.6 186,075 - EX31_RS09890 0.39 -2.3 186,075 - EX31_RS09890 0.39 -2.9 186,077 - EX31_RS09890 0.40 -2.3 186,077 - EX31_RS09890 0.40 -1.6 186,077 - EX31_RS09890 0.40 -1.6 186,126 - EX31_RS09890 0.45 -2.5 186,176 - EX31_RS09890 0.50 +0.0 186,203 - EX31_RS09890 0.53 -2.8 186,291 - EX31_RS09890 0.62 -1.7 186,330 - EX31_RS09890 0.66 -1.0 186,363 - EX31_RS09890 0.69 -0.7 186,363 - EX31_RS09890 0.69 +3.2 186,363 - EX31_RS09890 0.69 -2.9 186,380 - EX31_RS09890 0.71 -0.7 186,380 - EX31_RS09890 0.71 -2.3 186,400 + EX31_RS09890 0.73 +2.6 186,405 - EX31_RS09890 0.73 -1.7 186,405 - EX31_RS09890 0.73 -1.0 186,405 - EX31_RS09890 0.73 -3.4 186,405 - EX31_RS09890 0.73 +0.3 186,405 - EX31_RS09890 0.73 -3.7 186,405 - EX31_RS09890 0.73 -2.4 186,405 - EX31_RS09890 0.73 -2.9 186,441 - EX31_RS09890 0.77 -2.2 186,448 - EX31_RS09890 0.78 -1.7 186,448 - EX31_RS09890 0.78 -1.6 186,448 - EX31_RS09890 0.78 -1.0 186,448 - EX31_RS09890 0.78 -1.3 186,448 - EX31_RS09890 0.78 -2.5 186,587 - -0.7 186,587 - -1.4 186,587 - -2.5 186,627 - -1.4 186,666 - -0.2 186,672 + -1.4 186,672 + -0.4 186,673 - -0.2 186,673 - -1.6 186,673 - -4.8 186,673 - -1.7 186,673 - -1.2 186,860 + -1.1 186,860 + +1.3 186,861 - -0.4 186,861 - +0.7 186,861 - -1.1 186,875 + -2.1 186,876 - -0.2 186,920 + +0.3 186,921 - +0.6 186,921 - -1.2 186,934 + +0.4 186,935 - +1.3 186,942 - +1.6 187,003 - -0.4 187,003 - -0.2 187,003 - -0.3 187,003 - -1.1 187,018 - -2.9 187,040 - -1.0 187,040 - -1.1 187,040 - -1.6 187,040 - -3.1 187,095 - -0.4 187,095 - -1.0 187,095 - -1.2 187,095 - +0.0 187,095 - -0.9 187,095 - -1.0 187,099 - +0.7 187,099 - +0.6 187,099 - -0.4 187,099 - -1.2 187,099 - -1.9 187,099 - -0.3 187,099 - -1.0 187,139 - +0.6 187,139 - -0.6 187,309 - EX31_RS09895 0.11 -0.1 187,432 - EX31_RS09895 0.16 +0.0 187,432 - EX31_RS09895 0.16 +0.6 187,432 - EX31_RS09895 0.16 -1.1 187,432 - EX31_RS09895 0.16 -1.0 187,432 - EX31_RS09895 0.16 +0.0 187,436 - EX31_RS09895 0.16 -1.0 187,436 - EX31_RS09895 0.16 +0.0 187,504 - EX31_RS09895 0.19 -1.0
Or see this region's nucleotide sequence