Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09390 and EX31_RS25290 are separated by 63 nucleotides EX31_RS25290 and EX31_RS09395 are separated by 183 nucleotides EX31_RS09395 and EX31_RS09400 are separated by 170 nucleotides
EX31_RS09390: EX31_RS09390 - phosphate starvation-inducible protein PhoH, at 82,159 to 82,947
_RS09390
EX31_RS25290: EX31_RS25290 - hypothetical protein, at 83,011 to 83,424
_RS25290
EX31_RS09395: EX31_RS09395 - N-acetylmuramoyl-L-alanine amidase, at 83,608 to 84,756
_RS09395
EX31_RS09400: EX31_RS09400 - deferrochelatase/peroxidase EfeB, at 84,927 to 86,222
_RS09400
Position (kb)
83
84
85 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 82.721 kb on - strand, within EX31_RS09390 at 82.728 kb on + strand, within EX31_RS09390 at 82.728 kb on + strand, within EX31_RS09390 at 82.748 kb on + strand, within EX31_RS09390 at 82.749 kb on - strand, within EX31_RS09390 at 82.749 kb on - strand, within EX31_RS09390 at 82.749 kb on - strand, within EX31_RS09390 at 82.749 kb on - strand, within EX31_RS09390 at 82.838 kb on - strand, within EX31_RS09390 at 82.841 kb on - strand, within EX31_RS09390 at 82.841 kb on - strand, within EX31_RS09390 at 82.878 kb on + strand at 82.948 kb on - strand at 82.958 kb on + strand at 82.959 kb on - strand at 82.959 kb on - strand at 82.975 kb on - strand at 82.981 kb on - strand at 82.988 kb on - strand at 82.988 kb on - strand at 83.038 kb on + strand at 83.039 kb on - strand at 83.149 kb on + strand, within EX31_RS25290 at 83.202 kb on + strand, within EX31_RS25290 at 83.306 kb on - strand, within EX31_RS25290 at 83.318 kb on - strand, within EX31_RS25290 at 83.353 kb on + strand, within EX31_RS25290 at 83.389 kb on - strand at 83.443 kb on + strand at 83.444 kb on - strand at 83.444 kb on - strand at 83.444 kb on - strand at 83.462 kb on - strand at 83.462 kb on - strand at 83.485 kb on - strand at 83.486 kb on + strand at 83.487 kb on - strand at 83.487 kb on - strand at 83.487 kb on - strand at 83.487 kb on - strand at 83.487 kb on - strand at 83.487 kb on - strand at 83.497 kb on - strand at 83.497 kb on - strand at 83.497 kb on - strand at 83.498 kb on + strand at 83.499 kb on - strand at 83.499 kb on - strand at 83.557 kb on + strand at 83.558 kb on - strand at 83.623 kb on - strand at 83.645 kb on + strand at 83.646 kb on - strand at 83.829 kb on + strand, within EX31_RS09395 at 83.830 kb on - strand, within EX31_RS09395 at 83.832 kb on + strand, within EX31_RS09395 at 83.832 kb on + strand, within EX31_RS09395 at 83.833 kb on - strand, within EX31_RS09395 at 83.835 kb on + strand, within EX31_RS09395 at 83.835 kb on + strand, within EX31_RS09395 at 83.836 kb on - strand, within EX31_RS09395 at 83.836 kb on - strand, within EX31_RS09395 at 83.913 kb on + strand, within EX31_RS09395 at 83.918 kb on + strand, within EX31_RS09395 at 83.918 kb on + strand, within EX31_RS09395 at 83.948 kb on + strand, within EX31_RS09395 at 83.949 kb on - strand, within EX31_RS09395 at 83.984 kb on + strand, within EX31_RS09395 at 84.126 kb on - strand, within EX31_RS09395 at 84.136 kb on - strand, within EX31_RS09395 at 84.139 kb on + strand, within EX31_RS09395 at 84.236 kb on + strand, within EX31_RS09395 at 84.237 kb on - strand, within EX31_RS09395 at 84.237 kb on - strand, within EX31_RS09395 at 84.485 kb on - strand, within EX31_RS09395 at 84.555 kb on + strand, within EX31_RS09395 at 84.589 kb on - strand, within EX31_RS09395 at 84.602 kb on + strand, within EX31_RS09395 at 84.602 kb on + strand, within EX31_RS09395 at 84.602 kb on + strand, within EX31_RS09395 at 84.603 kb on - strand, within EX31_RS09395 at 84.684 kb on - strand at 84.757 kb on - strand at 84.846 kb on - strand at 84.924 kb on - strand at 84.955 kb on + strand at 85.017 kb on - strand at 85.017 kb on - strand at 85.018 kb on + strand at 85.018 kb on + strand at 85.018 kb on + strand at 85.018 kb on + strand at 85.018 kb on + strand at 85.018 kb on + strand at 85.019 kb on - strand at 85.019 kb on - strand at 85.019 kb on - strand at 85.019 kb on - strand at 85.019 kb on - strand at 85.145 kb on - strand, within EX31_RS09400 at 85.145 kb on - strand, within EX31_RS09400 at 85.145 kb on - strand, within EX31_RS09400 at 85.394 kb on - strand, within EX31_RS09400 at 85.559 kb on + strand, within EX31_RS09400 at 85.559 kb on + strand, within EX31_RS09400 at 85.591 kb on - strand, within EX31_RS09400 at 85.604 kb on - strand, within EX31_RS09400 at 85.638 kb on - strand, within EX31_RS09400 at 85.743 kb on - strand, within EX31_RS09400
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 82,721 - EX31_RS09390 0.71 +0.9 82,728 + EX31_RS09390 0.72 +0.8 82,728 + EX31_RS09390 0.72 +3.2 82,748 + EX31_RS09390 0.75 +0.6 82,749 - EX31_RS09390 0.75 +0.2 82,749 - EX31_RS09390 0.75 -0.2 82,749 - EX31_RS09390 0.75 -0.7 82,749 - EX31_RS09390 0.75 -2.0 82,838 - EX31_RS09390 0.86 +0.6 82,841 - EX31_RS09390 0.86 -0.0 82,841 - EX31_RS09390 0.86 +1.1 82,878 + +2.4 82,948 - +0.8 82,958 + -0.3 82,959 - +1.0 82,959 - -1.4 82,975 - -1.1 82,981 - -1.4 82,988 - +1.6 82,988 - -1.0 83,038 + -0.0 83,039 - +1.6 83,149 + EX31_RS25290 0.33 +0.7 83,202 + EX31_RS25290 0.46 +2.6 83,306 - EX31_RS25290 0.71 -0.2 83,318 - EX31_RS25290 0.74 +1.2 83,353 + EX31_RS25290 0.83 -0.2 83,389 - +0.5 83,443 + -0.9 83,444 - -1.6 83,444 - -1.6 83,444 - +0.6 83,462 - +0.9 83,462 - +0.2 83,485 - +0.4 83,486 + -1.6 83,487 - -0.2 83,487 - +0.7 83,487 - -0.1 83,487 - -0.7 83,487 - +0.1 83,487 - -0.5 83,497 - -0.4 83,497 - -0.0 83,497 - +0.2 83,498 + +0.6 83,499 - +0.3 83,499 - -0.2 83,557 + +0.8 83,558 - +1.6 83,623 - +2.3 83,645 + -0.7 83,646 - +0.7 83,829 + EX31_RS09395 0.19 -0.2 83,830 - EX31_RS09395 0.19 -0.0 83,832 + EX31_RS09395 0.19 -0.0 83,832 + EX31_RS09395 0.19 -0.0 83,833 - EX31_RS09395 0.20 +0.7 83,835 + EX31_RS09395 0.20 -0.3 83,835 + EX31_RS09395 0.20 -0.7 83,836 - EX31_RS09395 0.20 -0.4 83,836 - EX31_RS09395 0.20 -0.8 83,913 + EX31_RS09395 0.27 -1.0 83,918 + EX31_RS09395 0.27 +0.4 83,918 + EX31_RS09395 0.27 -0.3 83,948 + EX31_RS09395 0.30 +1.0 83,949 - EX31_RS09395 0.30 -0.7 83,984 + EX31_RS09395 0.33 +0.2 84,126 - EX31_RS09395 0.45 -0.3 84,136 - EX31_RS09395 0.46 +0.6 84,139 + EX31_RS09395 0.46 -0.0 84,236 + EX31_RS09395 0.55 +1.3 84,237 - EX31_RS09395 0.55 -0.1 84,237 - EX31_RS09395 0.55 +1.1 84,485 - EX31_RS09395 0.76 -1.7 84,555 + EX31_RS09395 0.82 -1.1 84,589 - EX31_RS09395 0.85 -0.4 84,602 + EX31_RS09395 0.87 +0.6 84,602 + EX31_RS09395 0.87 -2.0 84,602 + EX31_RS09395 0.87 +0.2 84,603 - EX31_RS09395 0.87 +0.4 84,684 - +0.6 84,757 - +1.1 84,846 - -0.3 84,924 - -0.0 84,955 + +1.0 85,017 - +0.4 85,017 - +0.4 85,018 + +0.7 85,018 + +0.3 85,018 + +0.1 85,018 + -0.9 85,018 + -0.5 85,018 + -2.0 85,019 - +1.6 85,019 - +1.6 85,019 - +0.2 85,019 - +1.0 85,019 - +0.7 85,145 - EX31_RS09400 0.17 +0.4 85,145 - EX31_RS09400 0.17 +1.3 85,145 - EX31_RS09400 0.17 +1.6 85,394 - EX31_RS09400 0.36 -0.9 85,559 + EX31_RS09400 0.49 +0.2 85,559 + EX31_RS09400 0.49 -0.5 85,591 - EX31_RS09400 0.51 +1.3 85,604 - EX31_RS09400 0.52 +0.8 85,638 - EX31_RS09400 0.55 -0.2 85,743 - EX31_RS09400 0.63 -0.7
Or see this region's nucleotide sequence