Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS07290 and EX31_RS07295 are separated by 63 nucleotides EX31_RS07295 and EX31_RS07300 are separated by 94 nucleotides EX31_RS07300 and EX31_RS07305 are separated by 2 nucleotides
EX31_RS07290: EX31_RS07290 - C4-dicarboxylic acid transporter DauA, at 260,968 to 262,635
_RS07290
EX31_RS07295: EX31_RS07295 - 3-deoxy-8-phosphooctulonate synthase, at 262,699 to 263,553
_RS07295
EX31_RS07300: EX31_RS07300 - tetratricopeptide repeat-containing protein, at 263,648 to 264,457
_RS07300
EX31_RS07305: EX31_RS07305 - siroheme synthase, at 264,460 to 264,849
_RS07305
Position (kb)
262
263
264 Strain fitness (log2 ratio)
-1
0
1
2 at 262.220 kb on + strand, within EX31_RS07290 at 262.328 kb on + strand, within EX31_RS07290 at 262.389 kb on + strand, within EX31_RS07290 at 262.390 kb on - strand, within EX31_RS07290 at 262.390 kb on - strand, within EX31_RS07290 at 262.442 kb on + strand, within EX31_RS07290 at 262.503 kb on + strand at 262.633 kb on + strand at 262.634 kb on - strand at 262.691 kb on - strand at 262.691 kb on - strand at 262.701 kb on - strand at 262.701 kb on - strand at 263.639 kb on - strand at 263.660 kb on - strand at 263.660 kb on - strand at 263.785 kb on - strand, within EX31_RS07300 at 263.812 kb on - strand, within EX31_RS07300 at 263.838 kb on - strand, within EX31_RS07300 at 264.008 kb on - strand, within EX31_RS07300 at 264.008 kb on - strand, within EX31_RS07300 at 264.083 kb on - strand, within EX31_RS07300 at 264.083 kb on - strand, within EX31_RS07300 at 264.206 kb on - strand, within EX31_RS07300 at 264.206 kb on - strand, within EX31_RS07300 at 264.345 kb on - strand, within EX31_RS07300 at 264.431 kb on - strand at 264.458 kb on - strand at 264.458 kb on - strand at 264.458 kb on - strand at 264.458 kb on - strand at 264.458 kb on - strand at 264.458 kb on - strand at 264.458 kb on - strand at 264.462 kb on - strand at 264.470 kb on - strand at 264.479 kb on - strand at 264.532 kb on - strand, within EX31_RS07305
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 262,220 + EX31_RS07290 0.75 +0.8 262,328 + EX31_RS07290 0.82 +1.1 262,389 + EX31_RS07290 0.85 -0.7 262,390 - EX31_RS07290 0.85 +0.6 262,390 - EX31_RS07290 0.85 +1.9 262,442 + EX31_RS07290 0.88 +0.5 262,503 + -0.6 262,633 + -1.7 262,634 - -0.3 262,691 - -0.6 262,691 - -1.4 262,701 - +0.6 262,701 - -0.7 263,639 - -1.4 263,660 - -0.3 263,660 - -1.0 263,785 - EX31_RS07300 0.17 +0.7 263,812 - EX31_RS07300 0.20 +0.3 263,838 - EX31_RS07300 0.23 +0.9 264,008 - EX31_RS07300 0.44 -0.6 264,008 - EX31_RS07300 0.44 +1.4 264,083 - EX31_RS07300 0.54 +0.6 264,083 - EX31_RS07300 0.54 -0.7 264,206 - EX31_RS07300 0.69 -1.6 264,206 - EX31_RS07300 0.69 -0.8 264,345 - EX31_RS07300 0.86 -0.7 264,431 - -1.6 264,458 - +0.8 264,458 - +1.1 264,458 - -0.7 264,458 - +0.4 264,458 - +0.1 264,458 - +1.0 264,458 - +0.3 264,462 - +0.7 264,470 - +0.8 264,479 - -0.3 264,532 - EX31_RS07305 0.18 +1.3
Or see this region's nucleotide sequence