Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03475 and EX31_RS03480 are separated by 13 nucleotides EX31_RS03480 and EX31_RS03485 are separated by 163 nucleotides
EX31_RS03475: EX31_RS03475 - pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase, at 185,961 to 187,859
_RS03475
EX31_RS03480: EX31_RS03480 - pyruvate dehydrogenase (acetyl-transferring), homodimeric type, at 187,873 to 190,536
_RS03480
EX31_RS03485: EX31_RS03485 - pyruvate dehydrogenase complex transcriptional repressor PdhR, at 190,700 to 191,470
_RS03485
Position (kb) 187
188
189
190
191 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 187.731 kb on - strand at 187.854 kb on - strand at 187.875 kb on - strand at 187.875 kb on - strand at 187.937 kb on - strand at 187.995 kb on - strand at 187.995 kb on - strand at 188.094 kb on - strand at 188.094 kb on - strand at 188.097 kb on + strand at 188.098 kb on - strand at 188.098 kb on - strand at 188.098 kb on - strand at 188.174 kb on + strand, within EX31_RS03480 at 188.175 kb on - strand, within EX31_RS03480 at 188.267 kb on + strand, within EX31_RS03480 at 188.282 kb on - strand, within EX31_RS03480 at 188.320 kb on + strand, within EX31_RS03480 at 188.321 kb on - strand, within EX31_RS03480 at 188.394 kb on - strand, within EX31_RS03480 at 188.394 kb on - strand, within EX31_RS03480 at 188.472 kb on - strand, within EX31_RS03480 at 188.472 kb on - strand, within EX31_RS03480 at 188.474 kb on + strand, within EX31_RS03480 at 188.475 kb on - strand, within EX31_RS03480 at 188.502 kb on - strand, within EX31_RS03480 at 188.505 kb on + strand, within EX31_RS03480 at 188.506 kb on - strand, within EX31_RS03480 at 188.644 kb on - strand, within EX31_RS03480 at 188.644 kb on - strand, within EX31_RS03480 at 188.644 kb on - strand, within EX31_RS03480 at 188.739 kb on - strand, within EX31_RS03480 at 188.742 kb on - strand, within EX31_RS03480 at 188.742 kb on - strand, within EX31_RS03480 at 188.747 kb on + strand, within EX31_RS03480 at 188.747 kb on + strand, within EX31_RS03480 at 188.748 kb on - strand, within EX31_RS03480 at 188.781 kb on - strand, within EX31_RS03480 at 188.868 kb on - strand, within EX31_RS03480 at 188.870 kb on + strand, within EX31_RS03480 at 188.871 kb on - strand, within EX31_RS03480 at 188.871 kb on - strand, within EX31_RS03480 at 188.871 kb on - strand, within EX31_RS03480 at 189.251 kb on - strand, within EX31_RS03480 at 189.251 kb on - strand, within EX31_RS03480 at 189.279 kb on - strand, within EX31_RS03480 at 189.328 kb on - strand, within EX31_RS03480 at 189.484 kb on - strand, within EX31_RS03480 at 189.539 kb on + strand, within EX31_RS03480 at 189.544 kb on - strand, within EX31_RS03480 at 189.926 kb on + strand, within EX31_RS03480 at 189.927 kb on - strand, within EX31_RS03480 at 189.927 kb on - strand, within EX31_RS03480 at 190.121 kb on + strand, within EX31_RS03480 at 190.281 kb on + strand at 190.297 kb on - strand at 190.350 kb on + strand at 190.351 kb on - strand at 190.358 kb on + strand at 190.359 kb on - strand at 190.550 kb on - strand at 190.560 kb on - strand at 190.562 kb on + strand at 190.563 kb on - strand at 190.563 kb on - strand at 190.563 kb on - strand at 190.563 kb on - strand at 190.563 kb on - strand at 190.652 kb on - strand at 190.652 kb on - strand at 190.652 kb on - strand at 190.652 kb on - strand at 190.652 kb on - strand at 190.652 kb on - strand at 190.652 kb on - strand at 190.652 kb on - strand at 190.734 kb on - strand at 190.734 kb on - strand at 190.824 kb on - strand, within EX31_RS03485 at 190.824 kb on - strand, within EX31_RS03485 at 190.824 kb on - strand, within EX31_RS03485 at 190.906 kb on - strand, within EX31_RS03485 at 190.955 kb on - strand, within EX31_RS03485 at 190.955 kb on - strand, within EX31_RS03485 at 190.985 kb on - strand, within EX31_RS03485 at 191.001 kb on - strand, within EX31_RS03485 at 191.014 kb on - strand, within EX31_RS03485 at 191.014 kb on - strand, within EX31_RS03485 at 191.014 kb on - strand, within EX31_RS03485 at 191.133 kb on + strand, within EX31_RS03485 at 191.134 kb on - strand, within EX31_RS03485 at 191.134 kb on - strand, within EX31_RS03485 at 191.205 kb on - strand, within EX31_RS03485 at 191.205 kb on - strand, within EX31_RS03485 at 191.243 kb on - strand, within EX31_RS03485 at 191.243 kb on - strand, within EX31_RS03485 at 191.243 kb on - strand, within EX31_RS03485 at 191.243 kb on - strand, within EX31_RS03485 at 191.243 kb on - strand, within EX31_RS03485 at 191.250 kb on - strand, within EX31_RS03485 at 191.250 kb on - strand, within EX31_RS03485 at 191.250 kb on - strand, within EX31_RS03485 at 191.250 kb on - strand, within EX31_RS03485 at 191.258 kb on - strand, within EX31_RS03485 at 191.258 kb on - strand, within EX31_RS03485 at 191.258 kb on - strand, within EX31_RS03485 at 191.258 kb on - strand, within EX31_RS03485 at 191.283 kb on - strand, within EX31_RS03485 at 191.283 kb on - strand, within EX31_RS03485 at 191.334 kb on - strand, within EX31_RS03485 at 191.334 kb on - strand, within EX31_RS03485 at 191.334 kb on - strand, within EX31_RS03485 at 191.444 kb on - strand at 191.444 kb on - strand at 191.444 kb on - strand at 191.444 kb on - strand at 191.444 kb on - strand at 191.444 kb on - strand at 191.444 kb on - strand at 191.444 kb on - strand at 191.444 kb on - strand at 191.444 kb on - strand at 191.444 kb on - strand at 191.464 kb on - strand at 191.464 kb on - strand at 191.464 kb on - strand at 191.464 kb on - strand at 191.464 kb on - strand at 191.464 kb on - strand at 191.464 kb on - strand at 191.464 kb on - strand at 191.464 kb on - strand at 191.471 kb on - strand at 191.471 kb on - strand at 191.471 kb on - strand at 191.471 kb on - strand at 191.471 kb on - strand at 191.471 kb on - strand at 191.471 kb on - strand at 191.471 kb on - strand at 191.471 kb on - strand at 191.471 kb on - strand at 191.471 kb on - strand at 191.471 kb on - strand at 191.471 kb on - strand at 191.471 kb on - strand at 191.471 kb on - strand at 191.472 kb on + strand at 191.472 kb on + strand at 191.472 kb on + strand at 191.472 kb on + strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.473 kb on - strand at 191.492 kb on + strand at 191.505 kb on - strand at 191.526 kb on - strand at 191.526 kb on - strand at 191.529 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 187,731 - -1.0 187,854 - -1.7 187,875 - +0.2 187,875 - +0.2 187,937 - -0.6 187,995 - +2.8 187,995 - -1.3 188,094 - -1.6 188,094 - -1.4 188,097 + -0.6 188,098 - +1.2 188,098 - -0.8 188,098 - -0.8 188,174 + EX31_RS03480 0.11 -0.1 188,175 - EX31_RS03480 0.11 +0.2 188,267 + EX31_RS03480 0.15 +1.6 188,282 - EX31_RS03480 0.15 -0.4 188,320 + EX31_RS03480 0.17 +1.2 188,321 - EX31_RS03480 0.17 -0.8 188,394 - EX31_RS03480 0.20 +2.2 188,394 - EX31_RS03480 0.20 +1.2 188,472 - EX31_RS03480 0.22 +2.2 188,472 - EX31_RS03480 0.22 +1.2 188,474 + EX31_RS03480 0.23 +0.6 188,475 - EX31_RS03480 0.23 +1.6 188,502 - EX31_RS03480 0.24 +0.6 188,505 + EX31_RS03480 0.24 -0.1 188,506 - EX31_RS03480 0.24 +0.4 188,644 - EX31_RS03480 0.29 +0.8 188,644 - EX31_RS03480 0.29 -0.4 188,644 - EX31_RS03480 0.29 -0.8 188,739 - EX31_RS03480 0.33 +2.2 188,742 - EX31_RS03480 0.33 +0.6 188,742 - EX31_RS03480 0.33 +0.5 188,747 + EX31_RS03480 0.33 -1.3 188,747 + EX31_RS03480 0.33 +2.2 188,748 - EX31_RS03480 0.33 -1.7 188,781 - EX31_RS03480 0.34 +0.2 188,868 - EX31_RS03480 0.37 +0.2 188,870 + EX31_RS03480 0.37 +0.2 188,871 - EX31_RS03480 0.37 -2.4 188,871 - EX31_RS03480 0.37 -0.1 188,871 - EX31_RS03480 0.37 -0.4 189,251 - EX31_RS03480 0.52 +0.2 189,251 - EX31_RS03480 0.52 -0.1 189,279 - EX31_RS03480 0.53 -1.5 189,328 - EX31_RS03480 0.55 +0.6 189,484 - EX31_RS03480 0.60 +0.9 189,539 + EX31_RS03480 0.63 +1.2 189,544 - EX31_RS03480 0.63 -0.6 189,926 + EX31_RS03480 0.77 +0.2 189,927 - EX31_RS03480 0.77 -0.1 189,927 - EX31_RS03480 0.77 -0.8 190,121 + EX31_RS03480 0.84 +2.2 190,281 + -0.1 190,297 - +0.2 190,350 + +3.2 190,351 - -0.4 190,358 + +0.2 190,359 - -1.0 190,550 - +0.3 190,560 - -0.5 190,562 + -1.7 190,563 - -1.8 190,563 - +0.1 190,563 - +1.5 190,563 - +0.8 190,563 - +0.2 190,652 - +0.5 190,652 - -0.7 190,652 - -0.7 190,652 - +0.2 190,652 - +0.2 190,652 - +0.5 190,652 - +0.5 190,652 - -0.4 190,734 - -0.4 190,734 - +0.2 190,824 - EX31_RS03485 0.16 +0.0 190,824 - EX31_RS03485 0.16 +0.5 190,824 - EX31_RS03485 0.16 +1.0 190,906 - EX31_RS03485 0.27 -0.0 190,955 - EX31_RS03485 0.33 +0.6 190,955 - EX31_RS03485 0.33 +0.7 190,985 - EX31_RS03485 0.37 +0.3 191,001 - EX31_RS03485 0.39 -0.6 191,014 - EX31_RS03485 0.41 +0.5 191,014 - EX31_RS03485 0.41 +0.6 191,014 - EX31_RS03485 0.41 +0.0 191,133 + EX31_RS03485 0.56 +1.4 191,134 - EX31_RS03485 0.56 +0.2 191,134 - EX31_RS03485 0.56 -0.3 191,205 - EX31_RS03485 0.65 -0.8 191,205 - EX31_RS03485 0.65 +0.1 191,243 - EX31_RS03485 0.70 -0.1 191,243 - EX31_RS03485 0.70 +0.1 191,243 - EX31_RS03485 0.70 -0.9 191,243 - EX31_RS03485 0.70 -0.3 191,243 - EX31_RS03485 0.70 -0.2 191,250 - EX31_RS03485 0.71 +0.0 191,250 - EX31_RS03485 0.71 -1.0 191,250 - EX31_RS03485 0.71 +0.9 191,250 - EX31_RS03485 0.71 -0.2 191,258 - EX31_RS03485 0.72 -0.1 191,258 - EX31_RS03485 0.72 +0.7 191,258 - EX31_RS03485 0.72 -0.3 191,258 - EX31_RS03485 0.72 +0.9 191,283 - EX31_RS03485 0.76 -0.6 191,283 - EX31_RS03485 0.76 +0.1 191,334 - EX31_RS03485 0.82 -1.3 191,334 - EX31_RS03485 0.82 +0.3 191,334 - EX31_RS03485 0.82 +0.2 191,444 - -0.4 191,444 - +0.4 191,444 - +0.3 191,444 - -1.7 191,444 - -0.7 191,444 - +0.9 191,444 - -0.7 191,444 - -0.7 191,444 - +0.7 191,444 - +0.3 191,444 - +0.5 191,464 - -0.2 191,464 - -0.3 191,464 - +0.1 191,464 - -0.2 191,464 - +0.1 191,464 - -0.4 191,464 - +0.4 191,464 - +0.0 191,464 - -1.2 191,471 - +0.6 191,471 - +0.0 191,471 - -0.0 191,471 - -0.0 191,471 - -0.8 191,471 - -0.1 191,471 - +0.2 191,471 - -0.3 191,471 - +0.8 191,471 - +0.2 191,471 - +0.6 191,471 - +0.5 191,471 - -0.2 191,471 - -0.2 191,471 - -1.3 191,472 + +0.2 191,472 + +1.2 191,472 + +0.6 191,472 + +0.2 191,473 - +1.1 191,473 - +0.5 191,473 - -0.0 191,473 - -0.0 191,473 - +0.9 191,473 - +0.4 191,473 - +0.1 191,473 - -0.3 191,473 - +0.2 191,473 - -0.8 191,473 - -0.1 191,473 - -0.3 191,473 - +0.9 191,473 - +0.5 191,473 - +0.6 191,473 - +0.1 191,473 - +0.2 191,473 - +1.1 191,473 - +0.4 191,473 - -0.7 191,473 - +1.6 191,473 - +0.7 191,473 - -0.5 191,473 - -1.1 191,473 - +0.6 191,473 - -1.6 191,473 - +0.1 191,473 - +0.2 191,473 - -0.6 191,473 - -0.1 191,473 - -0.0 191,473 - -0.1 191,473 - -0.6 191,473 - +0.0 191,473 - -0.0 191,473 - -0.6 191,473 - +0.3 191,473 - +0.3 191,473 - -0.6 191,473 - +0.4 191,473 - +0.6 191,473 - +0.7 191,473 - -1.1 191,473 - -1.5 191,473 - -0.0 191,473 - -1.2 191,492 + +2.2 191,505 - +0.7 191,526 - +0.5 191,526 - +0.2 191,529 - -0.1
Or see this region's nucleotide sequence