Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03205 and EX31_RS03210 are separated by 103 nucleotides EX31_RS03210 and EX31_RS03215 overlap by 4 nucleotides EX31_RS03215 and EX31_RS03220 are separated by 1 nucleotides
EX31_RS03205: EX31_RS03205 - glutamate-1-semialdehyde 2,1-aminomutase, at 126,659 to 127,939
_RS03205
EX31_RS03210: EX31_RS03210 - Fe(3+)-hydroxamate ABC transporter permease FhuB, at 128,043 to 130,031
_RS03210
EX31_RS03215: EX31_RS03215 - Fe(3+)-hydroxamate ABC transporter substrate-binding protein FhuD, at 130,028 to 130,930
_RS03215
EX31_RS03220: EX31_RS03220 - Fe3+-hydroxamate ABC transporter ATP-binding protein FhuC, at 130,932 to 131,744
_RS03220
Position (kb)
128
129
130
131 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 127.982 kb on + strand at 127.982 kb on + strand at 127.982 kb on + strand at 127.982 kb on + strand at 127.982 kb on + strand at 127.982 kb on + strand at 127.983 kb on - strand at 128.011 kb on + strand at 128.011 kb on + strand at 128.012 kb on - strand at 128.012 kb on - strand at 128.183 kb on + strand at 128.387 kb on + strand, within EX31_RS03210 at 128.391 kb on + strand, within EX31_RS03210 at 128.587 kb on - strand, within EX31_RS03210 at 128.591 kb on + strand, within EX31_RS03210 at 128.591 kb on + strand, within EX31_RS03210 at 128.591 kb on + strand, within EX31_RS03210 at 128.592 kb on - strand, within EX31_RS03210 at 128.592 kb on - strand, within EX31_RS03210 at 128.592 kb on - strand, within EX31_RS03210 at 128.819 kb on + strand, within EX31_RS03210 at 128.820 kb on - strand, within EX31_RS03210 at 128.820 kb on - strand, within EX31_RS03210 at 128.878 kb on - strand, within EX31_RS03210 at 129.045 kb on - strand, within EX31_RS03210 at 129.069 kb on + strand, within EX31_RS03210 at 129.261 kb on - strand, within EX31_RS03210 at 129.304 kb on - strand, within EX31_RS03210 at 129.502 kb on + strand, within EX31_RS03210 at 129.502 kb on + strand, within EX31_RS03210 at 129.502 kb on + strand, within EX31_RS03210 at 129.503 kb on - strand, within EX31_RS03210 at 129.503 kb on - strand, within EX31_RS03210 at 129.767 kb on + strand, within EX31_RS03210 at 129.780 kb on - strand, within EX31_RS03210 at 129.780 kb on - strand, within EX31_RS03210 at 129.784 kb on - strand, within EX31_RS03210 at 129.786 kb on + strand, within EX31_RS03210 at 129.786 kb on + strand, within EX31_RS03210 at 129.955 kb on + strand at 129.955 kb on + strand at 129.956 kb on - strand at 130.095 kb on + strand at 130.214 kb on + strand, within EX31_RS03215 at 130.229 kb on - strand, within EX31_RS03215 at 130.479 kb on - strand, within EX31_RS03215 at 130.502 kb on - strand, within EX31_RS03215 at 130.502 kb on - strand, within EX31_RS03215 at 130.637 kb on + strand, within EX31_RS03215 at 130.649 kb on - strand, within EX31_RS03215 at 130.728 kb on + strand, within EX31_RS03215 at 130.728 kb on + strand, within EX31_RS03215 at 130.728 kb on + strand, within EX31_RS03215 at 130.729 kb on - strand, within EX31_RS03215 at 130.729 kb on - strand, within EX31_RS03215 at 130.732 kb on + strand, within EX31_RS03215 at 130.733 kb on - strand, within EX31_RS03215 at 130.922 kb on - strand at 130.930 kb on + strand at 130.930 kb on + strand at 130.930 kb on + strand at 130.931 kb on - strand at 130.931 kb on - strand at 130.931 kb on - strand at 130.931 kb on - strand at 130.931 kb on - strand at 130.936 kb on + strand at 130.997 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 127,982 + +0.7 127,982 + -1.0 127,982 + -0.4 127,982 + -0.7 127,982 + +0.6 127,982 + -0.6 127,983 - +0.9 128,011 + -0.7 128,011 + +0.1 128,012 - -0.8 128,012 - -0.3 128,183 + -0.6 128,387 + EX31_RS03210 0.17 +0.4 128,391 + EX31_RS03210 0.17 +0.5 128,587 - EX31_RS03210 0.27 +0.8 128,591 + EX31_RS03210 0.28 -1.6 128,591 + EX31_RS03210 0.28 -0.6 128,591 + EX31_RS03210 0.28 -1.3 128,592 - EX31_RS03210 0.28 -0.8 128,592 - EX31_RS03210 0.28 +1.8 128,592 - EX31_RS03210 0.28 -0.7 128,819 + EX31_RS03210 0.39 -0.3 128,820 - EX31_RS03210 0.39 -0.7 128,820 - EX31_RS03210 0.39 -0.5 128,878 - EX31_RS03210 0.42 +0.1 129,045 - EX31_RS03210 0.50 -2.1 129,069 + EX31_RS03210 0.52 +2.2 129,261 - EX31_RS03210 0.61 -0.3 129,304 - EX31_RS03210 0.63 -0.2 129,502 + EX31_RS03210 0.73 -1.7 129,502 + EX31_RS03210 0.73 -0.4 129,502 + EX31_RS03210 0.73 +0.2 129,503 - EX31_RS03210 0.73 -0.2 129,503 - EX31_RS03210 0.73 -1.5 129,767 + EX31_RS03210 0.87 -0.1 129,780 - EX31_RS03210 0.87 +0.0 129,780 - EX31_RS03210 0.87 +0.9 129,784 - EX31_RS03210 0.88 -1.5 129,786 + EX31_RS03210 0.88 -0.6 129,786 + EX31_RS03210 0.88 +1.6 129,955 + -1.1 129,955 + +0.2 129,956 - -1.2 130,095 + +0.1 130,214 + EX31_RS03215 0.21 +0.2 130,229 - EX31_RS03215 0.22 +0.8 130,479 - EX31_RS03215 0.50 +0.3 130,502 - EX31_RS03215 0.52 +1.0 130,502 - EX31_RS03215 0.52 +0.5 130,637 + EX31_RS03215 0.67 -0.4 130,649 - EX31_RS03215 0.69 -0.4 130,728 + EX31_RS03215 0.78 +0.2 130,728 + EX31_RS03215 0.78 -0.6 130,728 + EX31_RS03215 0.78 -1.7 130,729 - EX31_RS03215 0.78 -0.0 130,729 - EX31_RS03215 0.78 -0.4 130,732 + EX31_RS03215 0.78 -0.6 130,733 - EX31_RS03215 0.78 +0.2 130,922 - -0.6 130,930 + -1.6 130,930 + -1.9 130,930 + +0.2 130,931 - -0.5 130,931 - +0.3 130,931 - -1.2 130,931 - +0.7 130,931 - -0.3 130,936 + +1.4 130,997 - -0.4
Or see this region's nucleotide sequence