Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03185 and EX31_RS03190 are separated by 325 nucleotides EX31_RS03190 and EX31_RS03195 are separated by 107 nucleotides EX31_RS03195 and EX31_RS03200 are separated by 103 nucleotides
EX31_RS03185: EX31_RS03185 - vitamin B12 ABC transporter substrate-binding protein BtuF, at 123,070 to 123,882
_RS03185
EX31_RS03190: EX31_RS03190 - hypothetical protein, at 124,208 to 124,453
_RS03190
EX31_RS03195: EX31_RS03195 - iron-sulfur cluster insertion protein ErpA, at 124,561 to 124,905
_RS03195
EX31_RS03200: EX31_RS03200 - H(+)/Cl(-) exchange transporter ClcA, at 125,009 to 126,472
_RS03200
Position (kb) 124
125 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 123.579 kb on + strand, within EX31_RS03185 at 123.615 kb on + strand, within EX31_RS03185 at 123.689 kb on + strand, within EX31_RS03185 at 123.689 kb on + strand, within EX31_RS03185 at 123.714 kb on + strand, within EX31_RS03185 at 123.715 kb on - strand, within EX31_RS03185 at 123.715 kb on - strand, within EX31_RS03185 at 123.825 kb on + strand at 123.826 kb on - strand at 123.826 kb on - strand at 124.014 kb on - strand at 124.014 kb on - strand at 124.083 kb on + strand at 124.186 kb on + strand at 124.186 kb on + strand at 124.186 kb on + strand at 124.186 kb on + strand at 124.187 kb on - strand at 124.187 kb on - strand at 124.187 kb on - strand at 124.187 kb on - strand at 124.200 kb on + strand at 124.200 kb on + strand at 124.201 kb on - strand at 124.201 kb on - strand at 124.206 kb on + strand at 124.207 kb on - strand at 124.207 kb on - strand at 124.221 kb on + strand at 124.221 kb on + strand at 124.222 kb on - strand at 124.222 kb on - strand at 124.381 kb on + strand, within EX31_RS03190 at 124.381 kb on + strand, within EX31_RS03190 at 124.399 kb on + strand, within EX31_RS03190 at 124.399 kb on + strand, within EX31_RS03190 at 124.400 kb on - strand, within EX31_RS03190 at 124.475 kb on - strand at 124.559 kb on - strand at 124.559 kb on - strand at 124.562 kb on + strand at 124.562 kb on + strand at 124.563 kb on - strand at 124.563 kb on - strand at 124.563 kb on - strand at 124.563 kb on - strand at 124.563 kb on - strand at 124.567 kb on - strand at 124.569 kb on + strand at 124.569 kb on + strand at 124.570 kb on - strand at 124.570 kb on - strand at 124.570 kb on - strand at 124.975 kb on - strand at 124.978 kb on - strand at 124.978 kb on - strand at 124.978 kb on - strand at 124.978 kb on - strand at 124.978 kb on - strand at 124.978 kb on - strand at 124.978 kb on - strand at 124.978 kb on - strand at 124.978 kb on - strand at 124.978 kb on - strand at 124.979 kb on + strand at 124.979 kb on + strand at 124.979 kb on + strand at 124.980 kb on - strand at 124.980 kb on - strand at 124.980 kb on - strand at 124.980 kb on - strand at 124.980 kb on - strand at 124.980 kb on - strand at 124.980 kb on - strand at 124.980 kb on - strand at 124.980 kb on - strand at 124.987 kb on - strand at 124.991 kb on + strand at 124.991 kb on + strand at 124.991 kb on + strand at 124.991 kb on + strand at 124.991 kb on + strand at 124.991 kb on + strand at 124.991 kb on + strand at 124.991 kb on + strand at 124.991 kb on + strand at 124.992 kb on - strand at 124.992 kb on - strand at 124.992 kb on - strand at 124.992 kb on - strand at 124.992 kb on - strand at 124.992 kb on - strand at 124.992 kb on - strand at 124.992 kb on - strand at 124.992 kb on - strand at 124.992 kb on - strand at 124.992 kb on - strand at 124.992 kb on - strand at 124.992 kb on - strand at 124.994 kb on - strand at 124.994 kb on - strand at 124.994 kb on - strand at 125.002 kb on + strand at 125.003 kb on - strand at 125.003 kb on - strand at 125.003 kb on - strand at 125.014 kb on + strand at 125.014 kb on + strand at 125.015 kb on - strand at 125.015 kb on - strand at 125.044 kb on + strand at 125.045 kb on - strand at 125.045 kb on - strand at 125.108 kb on + strand at 125.108 kb on + strand at 125.133 kb on + strand at 125.133 kb on + strand at 125.133 kb on + strand at 125.133 kb on + strand at 125.134 kb on - strand at 125.134 kb on - strand at 125.134 kb on - strand at 125.185 kb on + strand, within EX31_RS03200 at 125.186 kb on - strand, within EX31_RS03200 at 125.186 kb on - strand, within EX31_RS03200 at 125.186 kb on - strand, within EX31_RS03200 at 125.186 kb on - strand, within EX31_RS03200 at 125.186 kb on - strand, within EX31_RS03200 at 125.186 kb on - strand, within EX31_RS03200 at 125.211 kb on + strand, within EX31_RS03200 at 125.211 kb on + strand, within EX31_RS03200 at 125.212 kb on - strand, within EX31_RS03200 at 125.277 kb on - strand, within EX31_RS03200 at 125.322 kb on + strand, within EX31_RS03200 at 125.323 kb on - strand, within EX31_RS03200 at 125.437 kb on + strand, within EX31_RS03200 at 125.437 kb on + strand, within EX31_RS03200 at 125.484 kb on + strand, within EX31_RS03200 at 125.484 kb on + strand, within EX31_RS03200 at 125.633 kb on - strand, within EX31_RS03200 at 125.703 kb on + strand, within EX31_RS03200 at 125.703 kb on + strand, within EX31_RS03200 at 125.704 kb on - strand, within EX31_RS03200 at 125.704 kb on - strand, within EX31_RS03200 at 125.704 kb on - strand, within EX31_RS03200 at 125.704 kb on - strand, within EX31_RS03200 at 125.704 kb on - strand, within EX31_RS03200 at 125.704 kb on - strand, within EX31_RS03200 at 125.704 kb on - strand, within EX31_RS03200 at 125.704 kb on - strand, within EX31_RS03200 at 125.704 kb on - strand, within EX31_RS03200 at 125.704 kb on - strand, within EX31_RS03200 at 125.704 kb on - strand, within EX31_RS03200 at 125.716 kb on + strand, within EX31_RS03200 at 125.716 kb on + strand, within EX31_RS03200 at 125.717 kb on - strand, within EX31_RS03200 at 125.717 kb on - strand, within EX31_RS03200 at 125.776 kb on + strand, within EX31_RS03200 at 125.896 kb on + strand, within EX31_RS03200 at 125.896 kb on + strand, within EX31_RS03200
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 123,579 + EX31_RS03185 0.63 +0.5 123,615 + EX31_RS03185 0.67 -0.1 123,689 + EX31_RS03185 0.76 +0.8 123,689 + EX31_RS03185 0.76 -0.5 123,714 + EX31_RS03185 0.79 +2.2 123,715 - EX31_RS03185 0.79 +1.7 123,715 - EX31_RS03185 0.79 +1.2 123,825 + -0.3 123,826 - -1.8 123,826 - -0.1 124,014 - +0.7 124,014 - -0.1 124,083 + -1.0 124,186 + -0.3 124,186 + -0.3 124,186 + -0.4 124,186 + -0.6 124,187 - +0.4 124,187 - -0.0 124,187 - +0.3 124,187 - -0.6 124,200 + +0.6 124,200 + +0.4 124,201 - -0.7 124,201 - +1.3 124,206 + +1.1 124,207 - +0.1 124,207 - -0.5 124,221 + +0.0 124,221 + +0.7 124,222 - -0.9 124,222 - +2.2 124,381 + EX31_RS03190 0.70 +0.2 124,381 + EX31_RS03190 0.70 +0.6 124,399 + EX31_RS03190 0.78 +1.2 124,399 + EX31_RS03190 0.78 -0.7 124,400 - EX31_RS03190 0.78 +1.5 124,475 - +0.1 124,559 - +0.4 124,559 - +0.5 124,562 + -0.7 124,562 + -0.1 124,563 - +0.9 124,563 - +0.4 124,563 - +0.1 124,563 - +0.1 124,563 - +0.6 124,567 - -0.3 124,569 + -1.1 124,569 + -0.6 124,570 - -0.1 124,570 - -1.2 124,570 - -0.6 124,975 - +0.1 124,978 - -0.1 124,978 - +0.0 124,978 - -0.0 124,978 - +0.3 124,978 - -2.6 124,978 - -0.2 124,978 - -0.2 124,978 - +0.4 124,978 - -0.4 124,978 - +0.2 124,979 + -2.2 124,979 + -2.1 124,979 + +0.2 124,980 - +0.7 124,980 - -0.0 124,980 - +0.0 124,980 - +0.7 124,980 - -0.5 124,980 - -1.8 124,980 - +0.6 124,980 - -0.4 124,980 - -0.1 124,987 - -0.3 124,991 + -1.9 124,991 + -0.2 124,991 + -1.8 124,991 + -1.2 124,991 + -0.8 124,991 + +1.8 124,991 + -0.6 124,991 + -1.1 124,991 + -0.1 124,992 - -0.8 124,992 - -1.3 124,992 - +0.1 124,992 - -2.6 124,992 - +0.5 124,992 - -2.1 124,992 - +0.0 124,992 - +2.2 124,992 - +0.4 124,992 - -0.1 124,992 - +0.6 124,992 - +0.0 124,992 - -0.1 124,994 - +0.1 124,994 - +1.3 124,994 - +1.6 125,002 + -1.5 125,003 - +0.4 125,003 - -0.0 125,003 - +0.2 125,014 + +1.1 125,014 + +0.5 125,015 - -0.1 125,015 - +0.1 125,044 + +0.2 125,045 - -0.7 125,045 - +0.6 125,108 + +0.5 125,108 + +1.2 125,133 + -0.2 125,133 + -0.2 125,133 + -2.1 125,133 + +1.0 125,134 - -1.2 125,134 - +0.1 125,134 - -1.9 125,185 + EX31_RS03200 0.12 +0.9 125,186 - EX31_RS03200 0.12 -0.7 125,186 - EX31_RS03200 0.12 -2.5 125,186 - EX31_RS03200 0.12 +0.6 125,186 - EX31_RS03200 0.12 -0.7 125,186 - EX31_RS03200 0.12 +0.2 125,186 - EX31_RS03200 0.12 -0.5 125,211 + EX31_RS03200 0.14 +0.2 125,211 + EX31_RS03200 0.14 -1.8 125,212 - EX31_RS03200 0.14 +0.2 125,277 - EX31_RS03200 0.18 -0.1 125,322 + EX31_RS03200 0.21 +0.1 125,323 - EX31_RS03200 0.21 +0.1 125,437 + EX31_RS03200 0.29 +2.0 125,437 + EX31_RS03200 0.29 +0.9 125,484 + EX31_RS03200 0.32 -0.9 125,484 + EX31_RS03200 0.32 +2.8 125,633 - EX31_RS03200 0.43 -0.1 125,703 + EX31_RS03200 0.47 +1.2 125,703 + EX31_RS03200 0.47 +0.2 125,704 - EX31_RS03200 0.47 +0.4 125,704 - EX31_RS03200 0.47 +0.7 125,704 - EX31_RS03200 0.47 -1.5 125,704 - EX31_RS03200 0.47 +0.2 125,704 - EX31_RS03200 0.47 -0.6 125,704 - EX31_RS03200 0.47 -0.1 125,704 - EX31_RS03200 0.47 -0.2 125,704 - EX31_RS03200 0.47 +0.8 125,704 - EX31_RS03200 0.47 +0.3 125,704 - EX31_RS03200 0.47 +0.6 125,704 - EX31_RS03200 0.47 +0.3 125,716 + EX31_RS03200 0.48 +2.2 125,716 + EX31_RS03200 0.48 +0.6 125,717 - EX31_RS03200 0.48 +0.1 125,717 - EX31_RS03200 0.48 +1.1 125,776 + EX31_RS03200 0.52 +1.7 125,896 + EX31_RS03200 0.61 +1.7 125,896 + EX31_RS03200 0.61 +0.6
Or see this region's nucleotide sequence