Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02155 and EX31_RS02160 are separated by 275 nucleotides EX31_RS02160 and EX31_RS02165 overlap by 4 nucleotides EX31_RS02165 and EX31_RS02170 are separated by 200 nucleotides
EX31_RS02155: EX31_RS02155 - HD domain-containing protein, at 469,910 to 471,040
_RS02155
EX31_RS02160: EX31_RS02160 - helix-turn-helix domain-containing protein, at 471,316 to 472,098
_RS02160
EX31_RS02165: EX31_RS02165 - hypothetical protein, at 472,095 to 472,586
_RS02165
EX31_RS02170: EX31_RS02170 - porin, at 472,787 to 473,353
_RS02170
Position (kb) 471
472
473 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 470.333 kb on + strand, within EX31_RS02155 at 470.334 kb on - strand, within EX31_RS02155 at 470.416 kb on + strand, within EX31_RS02155 at 470.513 kb on + strand, within EX31_RS02155 at 470.514 kb on - strand, within EX31_RS02155 at 470.871 kb on - strand, within EX31_RS02155 at 470.944 kb on + strand at 470.944 kb on + strand at 470.944 kb on + strand at 470.945 kb on - strand at 470.945 kb on - strand at 470.945 kb on - strand at 470.946 kb on + strand at 470.946 kb on + strand at 470.946 kb on + strand at 470.946 kb on + strand at 470.947 kb on - strand at 470.947 kb on - strand at 470.947 kb on - strand at 470.947 kb on - strand at 470.947 kb on - strand at 470.947 kb on - strand at 470.984 kb on + strand at 470.985 kb on - strand at 471.031 kb on + strand at 471.032 kb on - strand at 471.032 kb on - strand at 471.042 kb on + strand at 471.043 kb on - strand at 471.121 kb on + strand at 471.121 kb on + strand at 471.121 kb on + strand at 471.121 kb on + strand at 471.121 kb on + strand at 471.121 kb on + strand at 471.121 kb on + strand at 471.121 kb on + strand at 471.122 kb on - strand at 471.122 kb on - strand at 471.122 kb on - strand at 471.122 kb on - strand at 471.122 kb on - strand at 471.122 kb on - strand at 471.123 kb on + strand at 471.123 kb on + strand at 471.123 kb on + strand at 471.123 kb on + strand at 471.123 kb on + strand at 471.123 kb on + strand at 471.123 kb on + strand at 471.123 kb on + strand at 471.123 kb on + strand at 471.123 kb on + strand at 471.123 kb on + strand at 471.124 kb on - strand at 471.124 kb on - strand at 471.124 kb on - strand at 471.124 kb on - strand at 471.124 kb on - strand at 471.125 kb on + strand at 471.126 kb on - strand at 471.127 kb on + strand at 471.128 kb on - strand at 471.128 kb on - strand at 471.163 kb on + strand at 471.163 kb on + strand at 471.166 kb on + strand at 471.166 kb on + strand at 471.166 kb on + strand at 471.166 kb on + strand at 471.167 kb on - strand at 471.167 kb on - strand at 471.205 kb on + strand at 471.206 kb on - strand at 471.206 kb on - strand at 471.206 kb on - strand at 471.236 kb on + strand at 471.236 kb on + strand at 471.243 kb on + strand at 471.243 kb on + strand at 471.243 kb on + strand at 471.243 kb on + strand at 471.243 kb on + strand at 471.244 kb on - strand at 471.244 kb on - strand at 471.244 kb on - strand at 471.244 kb on - strand at 471.245 kb on + strand at 471.246 kb on - strand at 471.248 kb on + strand at 471.248 kb on + strand at 471.248 kb on + strand at 471.251 kb on + strand at 471.589 kb on - strand, within EX31_RS02160 at 471.610 kb on - strand, within EX31_RS02160 at 471.750 kb on + strand, within EX31_RS02160 at 471.858 kb on + strand, within EX31_RS02160 at 471.951 kb on + strand, within EX31_RS02160 at 471.952 kb on - strand, within EX31_RS02160 at 471.970 kb on + strand, within EX31_RS02160 at 471.970 kb on + strand, within EX31_RS02160 at 471.971 kb on - strand, within EX31_RS02160 at 471.972 kb on + strand, within EX31_RS02160 at 471.984 kb on - strand, within EX31_RS02160 at 472.041 kb on + strand at 472.041 kb on + strand at 472.041 kb on + strand at 472.041 kb on + strand at 472.042 kb on - strand at 472.044 kb on + strand at 472.053 kb on + strand at 472.078 kb on + strand at 472.078 kb on + strand at 472.080 kb on + strand at 472.080 kb on + strand at 472.080 kb on + strand at 472.081 kb on - strand at 472.081 kb on - strand at 472.123 kb on - strand at 472.167 kb on + strand, within EX31_RS02165 at 472.168 kb on - strand, within EX31_RS02165 at 472.187 kb on + strand, within EX31_RS02165 at 472.194 kb on - strand, within EX31_RS02165 at 472.301 kb on + strand, within EX31_RS02165 at 472.317 kb on + strand, within EX31_RS02165 at 472.321 kb on + strand, within EX31_RS02165 at 472.321 kb on + strand, within EX31_RS02165 at 472.344 kb on + strand, within EX31_RS02165 at 472.344 kb on + strand, within EX31_RS02165 at 472.344 kb on + strand, within EX31_RS02165 at 472.344 kb on + strand, within EX31_RS02165 at 472.345 kb on - strand, within EX31_RS02165 at 472.345 kb on - strand, within EX31_RS02165 at 472.345 kb on - strand, within EX31_RS02165 at 472.345 kb on - strand, within EX31_RS02165 at 472.392 kb on - strand, within EX31_RS02165 at 472.392 kb on - strand, within EX31_RS02165 at 472.399 kb on - strand, within EX31_RS02165 at 472.536 kb on + strand, within EX31_RS02165 at 472.536 kb on + strand, within EX31_RS02165 at 472.536 kb on + strand, within EX31_RS02165 at 472.536 kb on + strand, within EX31_RS02165 at 472.537 kb on - strand at 472.537 kb on - strand at 472.537 kb on - strand at 472.550 kb on + strand at 472.554 kb on + strand at 472.651 kb on + strand at 472.651 kb on + strand at 472.668 kb on + strand at 472.670 kb on + strand at 472.702 kb on - strand at 472.776 kb on + strand at 472.781 kb on - strand at 472.792 kb on + strand at 472.795 kb on + strand at 472.795 kb on + strand at 472.795 kb on + strand at 472.795 kb on + strand at 472.796 kb on - strand at 472.841 kb on - strand at 472.841 kb on - strand at 473.011 kb on + strand, within EX31_RS02170 at 473.039 kb on - strand, within EX31_RS02170 at 473.039 kb on - strand, within EX31_RS02170 at 473.060 kb on + strand, within EX31_RS02170 at 473.060 kb on + strand, within EX31_RS02170 at 473.061 kb on - strand, within EX31_RS02170 at 473.062 kb on + strand, within EX31_RS02170 at 473.063 kb on - strand, within EX31_RS02170 at 473.074 kb on + strand, within EX31_RS02170 at 473.074 kb on + strand, within EX31_RS02170 at 473.083 kb on + strand, within EX31_RS02170
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 470,333 + EX31_RS02155 0.37 +0.3 470,334 - EX31_RS02155 0.37 -0.1 470,416 + EX31_RS02155 0.45 -0.4 470,513 + EX31_RS02155 0.53 -0.6 470,514 - EX31_RS02155 0.53 -0.1 470,871 - EX31_RS02155 0.85 -0.4 470,944 + -0.9 470,944 + -2.2 470,944 + +0.7 470,945 - +3.3 470,945 - +0.0 470,945 - +1.6 470,946 + -0.5 470,946 + -0.3 470,946 + +2.5 470,946 + +0.4 470,947 - +0.6 470,947 - +0.8 470,947 - +0.2 470,947 - -0.7 470,947 - +1.6 470,947 - -0.5 470,984 + +0.3 470,985 - +0.6 471,031 + +0.1 471,032 - +1.2 471,032 - -0.2 471,042 + +0.9 471,043 - -0.0 471,121 + +0.9 471,121 + -0.1 471,121 + +1.3 471,121 + +1.3 471,121 + -0.1 471,121 + -0.8 471,121 + -0.5 471,121 + +0.3 471,122 - +0.7 471,122 - +0.2 471,122 - +1.2 471,122 - -0.0 471,122 - -0.4 471,122 - -0.5 471,123 + +0.3 471,123 + +1.2 471,123 + -1.1 471,123 + -1.8 471,123 + +0.8 471,123 + -0.1 471,123 + +0.9 471,123 + +0.7 471,123 + +0.2 471,123 + +0.2 471,123 + -0.4 471,124 - -1.5 471,124 - -0.4 471,124 - +0.1 471,124 - +0.9 471,124 - +1.1 471,125 + +0.6 471,126 - +1.3 471,127 + -1.2 471,128 - -0.1 471,128 - +1.3 471,163 + -0.7 471,163 + -0.4 471,166 + +0.5 471,166 + -0.4 471,166 + +0.3 471,166 + -0.7 471,167 - +0.8 471,167 - +1.2 471,205 + +1.9 471,206 - +0.6 471,206 - -2.2 471,206 - +0.8 471,236 + +0.7 471,236 + -0.1 471,243 + +0.6 471,243 + +0.2 471,243 + -1.1 471,243 + -0.1 471,243 + +0.9 471,244 - -1.7 471,244 - +0.5 471,244 - +2.5 471,244 - -0.2 471,245 + +0.7 471,246 - -0.4 471,248 + -0.4 471,248 + -0.8 471,248 + -0.8 471,251 + +0.4 471,589 - EX31_RS02160 0.35 -1.8 471,610 - EX31_RS02160 0.38 -0.4 471,750 + EX31_RS02160 0.55 +0.9 471,858 + EX31_RS02160 0.69 -0.7 471,951 + EX31_RS02160 0.81 -0.1 471,952 - EX31_RS02160 0.81 -2.2 471,970 + EX31_RS02160 0.84 +0.8 471,970 + EX31_RS02160 0.84 -1.0 471,971 - EX31_RS02160 0.84 -1.4 471,972 + EX31_RS02160 0.84 -0.2 471,984 - EX31_RS02160 0.85 -0.1 472,041 + +0.7 472,041 + +0.1 472,041 + +0.2 472,041 + +1.9 472,042 - -1.7 472,044 + +1.9 472,053 + +0.3 472,078 + -0.2 472,078 + -0.6 472,080 + +0.3 472,080 + -0.2 472,080 + +0.3 472,081 - +1.8 472,081 - +1.5 472,123 - -0.6 472,167 + EX31_RS02165 0.15 -0.6 472,168 - EX31_RS02165 0.15 -1.1 472,187 + EX31_RS02165 0.19 +0.9 472,194 - EX31_RS02165 0.20 -0.4 472,301 + EX31_RS02165 0.42 +0.7 472,317 + EX31_RS02165 0.45 -0.1 472,321 + EX31_RS02165 0.46 -0.2 472,321 + EX31_RS02165 0.46 +1.3 472,344 + EX31_RS02165 0.51 +0.3 472,344 + EX31_RS02165 0.51 -0.8 472,344 + EX31_RS02165 0.51 -0.9 472,344 + EX31_RS02165 0.51 -0.1 472,345 - EX31_RS02165 0.51 -1.2 472,345 - EX31_RS02165 0.51 -0.1 472,345 - EX31_RS02165 0.51 +1.9 472,345 - EX31_RS02165 0.51 +0.9 472,392 - EX31_RS02165 0.60 +0.1 472,392 - EX31_RS02165 0.60 -0.1 472,399 - EX31_RS02165 0.62 -1.4 472,536 + EX31_RS02165 0.90 +0.1 472,536 + EX31_RS02165 0.90 +0.0 472,536 + EX31_RS02165 0.90 +0.7 472,536 + EX31_RS02165 0.90 +1.3 472,537 - +2.2 472,537 - -0.8 472,537 - +1.9 472,550 + -0.4 472,554 + +1.3 472,651 + +0.7 472,651 + +1.6 472,668 + +1.1 472,670 + +0.8 472,702 - +1.3 472,776 + +0.3 472,781 - -1.2 472,792 + +0.1 472,795 + -0.7 472,795 + -0.4 472,795 + +0.0 472,795 + +1.0 472,796 - -1.4 472,841 - -0.1 472,841 - -0.5 473,011 + EX31_RS02170 0.40 -0.4 473,039 - EX31_RS02170 0.44 -0.3 473,039 - EX31_RS02170 0.44 -0.4 473,060 + EX31_RS02170 0.48 +1.2 473,060 + EX31_RS02170 0.48 -0.4 473,061 - EX31_RS02170 0.48 +0.9 473,062 + EX31_RS02170 0.49 -0.7 473,063 - EX31_RS02170 0.49 -2.5 473,074 + EX31_RS02170 0.51 +0.4 473,074 + EX31_RS02170 0.51 +0.4 473,083 + EX31_RS02170 0.52 +0.3
Or see this region's nucleotide sequence