Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01300 and EX31_RS01305 overlap by 4 nucleotides EX31_RS01305 and EX31_RS01310 are separated by 193 nucleotides EX31_RS01310 and EX31_RS01315 are separated by 31 nucleotides
EX31_RS01300: EX31_RS01300 - alpha,alpha-trehalose-phosphate synthase, at 288,310 to 289,716
_RS01300
EX31_RS01305: EX31_RS01305 - trehalose-phosphatase, at 289,713 to 290,495
_RS01305
EX31_RS01310: EX31_RS01310 - DUF2493 domain-containing protein, at 290,689 to 291,054
_RS01310
EX31_RS01315: EX31_RS01315 - Na/Pi cotransporter family protein, at 291,086 to 292,759
_RS01315
Position (kb) 289
290
291 Strain fitness (log2 ratio) -2
-1
0
1
2 at 288.734 kb on + strand, within EX31_RS01300 at 288.735 kb on - strand, within EX31_RS01300 at 288.738 kb on + strand, within EX31_RS01300 at 288.789 kb on - strand, within EX31_RS01300 at 289.011 kb on + strand, within EX31_RS01300 at 289.038 kb on - strand, within EX31_RS01300 at 289.040 kb on - strand, within EX31_RS01300 at 289.162 kb on + strand, within EX31_RS01300 at 289.169 kb on + strand, within EX31_RS01300 at 289.258 kb on + strand, within EX31_RS01300 at 289.259 kb on - strand, within EX31_RS01300 at 289.306 kb on + strand, within EX31_RS01300 at 289.306 kb on + strand, within EX31_RS01300 at 289.307 kb on - strand, within EX31_RS01300 at 289.332 kb on + strand, within EX31_RS01300 at 289.333 kb on - strand, within EX31_RS01300 at 289.431 kb on + strand, within EX31_RS01300 at 289.478 kb on + strand, within EX31_RS01300 at 289.482 kb on - strand, within EX31_RS01300 at 289.482 kb on - strand, within EX31_RS01300 at 289.521 kb on - strand, within EX31_RS01300 at 289.524 kb on + strand, within EX31_RS01300 at 289.524 kb on + strand, within EX31_RS01300 at 289.524 kb on + strand, within EX31_RS01300 at 289.573 kb on + strand, within EX31_RS01300 at 289.582 kb on + strand at 289.583 kb on - strand at 289.583 kb on - strand at 289.583 kb on - strand at 289.617 kb on + strand at 289.617 kb on + strand at 289.618 kb on - strand at 289.734 kb on - strand at 289.734 kb on - strand at 289.746 kb on - strand at 289.746 kb on - strand at 289.746 kb on - strand at 289.746 kb on - strand at 289.755 kb on + strand at 289.755 kb on + strand at 289.756 kb on - strand at 289.911 kb on - strand, within EX31_RS01305 at 289.911 kb on - strand, within EX31_RS01305 at 289.946 kb on - strand, within EX31_RS01305 at 289.962 kb on - strand, within EX31_RS01305 at 289.962 kb on - strand, within EX31_RS01305 at 290.052 kb on + strand, within EX31_RS01305 at 290.053 kb on - strand, within EX31_RS01305 at 290.055 kb on + strand, within EX31_RS01305 at 290.056 kb on - strand, within EX31_RS01305 at 290.056 kb on - strand, within EX31_RS01305 at 290.440 kb on + strand at 290.530 kb on + strand at 290.530 kb on + strand at 290.531 kb on - strand at 290.584 kb on - strand at 290.584 kb on - strand at 290.584 kb on - strand at 290.584 kb on - strand at 290.584 kb on - strand at 290.587 kb on + strand at 290.587 kb on + strand at 290.587 kb on + strand at 290.588 kb on - strand at 290.588 kb on - strand at 290.588 kb on - strand at 290.588 kb on - strand at 290.588 kb on - strand at 290.588 kb on - strand at 290.590 kb on - strand at 290.590 kb on - strand at 290.590 kb on - strand at 290.823 kb on + strand, within EX31_RS01310 at 290.824 kb on - strand, within EX31_RS01310 at 290.840 kb on - strand, within EX31_RS01310 at 291.015 kb on + strand, within EX31_RS01310 at 291.016 kb on - strand, within EX31_RS01310 at 291.016 kb on - strand, within EX31_RS01310 at 291.016 kb on - strand, within EX31_RS01310 at 291.059 kb on + strand at 291.059 kb on + strand at 291.060 kb on - strand at 291.060 kb on - strand at 291.060 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 288,734 + EX31_RS01300 0.30 +0.6 288,735 - EX31_RS01300 0.30 +1.6 288,738 + EX31_RS01300 0.30 -0.5 288,789 - EX31_RS01300 0.34 -0.2 289,011 + EX31_RS01300 0.50 +0.9 289,038 - EX31_RS01300 0.52 -0.1 289,040 - EX31_RS01300 0.52 +1.5 289,162 + EX31_RS01300 0.61 -1.1 289,169 + EX31_RS01300 0.61 +0.3 289,258 + EX31_RS01300 0.67 +0.3 289,259 - EX31_RS01300 0.67 +0.0 289,306 + EX31_RS01300 0.71 +0.0 289,306 + EX31_RS01300 0.71 +0.3 289,307 - EX31_RS01300 0.71 -0.7 289,332 + EX31_RS01300 0.73 -0.1 289,333 - EX31_RS01300 0.73 +1.3 289,431 + EX31_RS01300 0.80 +1.0 289,478 + EX31_RS01300 0.83 -0.2 289,482 - EX31_RS01300 0.83 -1.7 289,482 - EX31_RS01300 0.83 -0.8 289,521 - EX31_RS01300 0.86 -0.1 289,524 + EX31_RS01300 0.86 +1.1 289,524 + EX31_RS01300 0.86 +1.5 289,524 + EX31_RS01300 0.86 -0.7 289,573 + EX31_RS01300 0.90 -0.7 289,582 + +0.4 289,583 - +0.6 289,583 - -0.2 289,583 - -0.1 289,617 + -0.1 289,617 + +2.5 289,618 - -0.7 289,734 - +1.3 289,734 - -0.0 289,746 - -0.6 289,746 - +0.6 289,746 - -0.1 289,746 - +0.3 289,755 + +1.1 289,755 + -0.2 289,756 - -0.7 289,911 - EX31_RS01305 0.25 -0.8 289,911 - EX31_RS01305 0.25 -2.4 289,946 - EX31_RS01305 0.30 -2.2 289,962 - EX31_RS01305 0.32 -0.2 289,962 - EX31_RS01305 0.32 -0.1 290,052 + EX31_RS01305 0.43 +1.9 290,053 - EX31_RS01305 0.43 -0.6 290,055 + EX31_RS01305 0.44 -0.2 290,056 - EX31_RS01305 0.44 -1.1 290,056 - EX31_RS01305 0.44 +0.2 290,440 + +1.5 290,530 + +0.3 290,530 + +0.7 290,531 - -0.4 290,584 - -1.4 290,584 - -0.2 290,584 - -0.7 290,584 - +0.8 290,584 - +2.4 290,587 + -1.4 290,587 + +0.5 290,587 + +0.9 290,588 - -0.4 290,588 - +0.7 290,588 - +0.6 290,588 - -0.8 290,588 - +0.0 290,588 - +1.2 290,590 - -0.7 290,590 - -0.1 290,590 - +0.1 290,823 + EX31_RS01310 0.37 -2.3 290,824 - EX31_RS01310 0.37 +0.3 290,840 - EX31_RS01310 0.41 +0.7 291,015 + EX31_RS01310 0.89 +0.6 291,016 - EX31_RS01310 0.89 +0.8 291,016 - EX31_RS01310 0.89 -1.7 291,016 - EX31_RS01310 0.89 +1.5 291,059 + -1.1 291,059 + -1.1 291,060 - +1.2 291,060 - +0.4 291,060 - -1.9
Or see this region's nucleotide sequence