Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01080 and EX31_RS01085 overlap by 4 nucleotides EX31_RS01085 and EX31_RS01090 overlap by 4 nucleotides EX31_RS01090 and EX31_RS01095 overlap by 4 nucleotides EX31_RS01095 and EX31_RS01100 are separated by 139 nucleotides
EX31_RS01080: EX31_RS01080 - metal ABC transporter substrate-binding protein, at 235,343 to 236,284
_RS01080
EX31_RS01085: EX31_RS01085 - ATP-binding cassette domain-containing protein, at 236,281 to 237,060
_RS01085
EX31_RS01090: EX31_RS01090 - metal ABC transporter permease, at 237,057 to 237,911
_RS01090
EX31_RS01095: EX31_RS01095 - metal ABC transporter permease, at 237,908 to 238,762
_RS01095
EX31_RS01100: EX31_RS01100 - hypothetical protein, at 238,902 to 239,081
_RS01100
Position (kb) 237
238 Strain fitness (log2 ratio) -2
-1
0
1
2 at 236.186 kb on + strand, within EX31_RS01080 at 236.186 kb on + strand, within EX31_RS01080 at 236.186 kb on + strand, within EX31_RS01080 at 236.186 kb on + strand, within EX31_RS01080 at 236.228 kb on + strand at 236.228 kb on + strand at 236.228 kb on + strand at 236.228 kb on + strand at 236.229 kb on - strand at 236.316 kb on + strand at 236.316 kb on + strand at 236.329 kb on + strand at 236.329 kb on + strand at 236.329 kb on + strand at 236.329 kb on + strand at 236.329 kb on + strand at 236.329 kb on + strand at 236.418 kb on + strand, within EX31_RS01085 at 236.433 kb on + strand, within EX31_RS01085 at 236.433 kb on + strand, within EX31_RS01085 at 236.527 kb on + strand, within EX31_RS01085 at 236.527 kb on + strand, within EX31_RS01085 at 236.528 kb on - strand, within EX31_RS01085 at 236.641 kb on - strand, within EX31_RS01085 at 236.965 kb on - strand, within EX31_RS01085 at 237.012 kb on + strand at 237.012 kb on + strand at 237.088 kb on - strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.093 kb on + strand at 237.094 kb on - strand at 237.095 kb on + strand at 237.095 kb on + strand at 237.096 kb on - strand at 237.276 kb on - strand, within EX31_RS01090 at 237.379 kb on + strand, within EX31_RS01090 at 237.434 kb on + strand, within EX31_RS01090 at 237.463 kb on + strand, within EX31_RS01090 at 237.476 kb on + strand, within EX31_RS01090 at 237.509 kb on + strand, within EX31_RS01090 at 237.602 kb on + strand, within EX31_RS01090 at 237.631 kb on + strand, within EX31_RS01090 at 237.691 kb on - strand, within EX31_RS01090 at 237.778 kb on - strand, within EX31_RS01090 at 237.890 kb on + strand at 237.956 kb on - strand at 238.080 kb on + strand, within EX31_RS01095 at 238.091 kb on + strand, within EX31_RS01095 at 238.219 kb on + strand, within EX31_RS01095 at 238.434 kb on + strand, within EX31_RS01095 at 238.448 kb on + strand, within EX31_RS01095 at 238.449 kb on - strand, within EX31_RS01095 at 238.462 kb on + strand, within EX31_RS01095 at 238.462 kb on + strand, within EX31_RS01095 at 238.462 kb on + strand, within EX31_RS01095 at 238.462 kb on + strand, within EX31_RS01095 at 238.462 kb on + strand, within EX31_RS01095 at 238.462 kb on + strand, within EX31_RS01095 at 238.463 kb on - strand, within EX31_RS01095 at 238.640 kb on + strand, within EX31_RS01095 at 238.640 kb on + strand, within EX31_RS01095 at 238.640 kb on + strand, within EX31_RS01095 at 238.640 kb on + strand, within EX31_RS01095 at 238.641 kb on - strand, within EX31_RS01095 at 238.641 kb on - strand, within EX31_RS01095 at 238.641 kb on - strand, within EX31_RS01095 at 238.641 kb on - strand, within EX31_RS01095 at 238.642 kb on + strand, within EX31_RS01095 at 238.642 kb on + strand, within EX31_RS01095 at 238.756 kb on + strand at 238.760 kb on + strand at 238.760 kb on + strand at 238.760 kb on + strand at 238.841 kb on - strand at 238.884 kb on + strand at 238.884 kb on + strand at 238.885 kb on - strand at 238.901 kb on + strand at 238.901 kb on + strand at 238.901 kb on + strand at 238.901 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 236,186 + EX31_RS01080 0.89 -0.9 236,186 + EX31_RS01080 0.89 +0.5 236,186 + EX31_RS01080 0.89 +0.0 236,186 + EX31_RS01080 0.89 -0.1 236,228 + +0.3 236,228 + -0.6 236,228 + -0.5 236,228 + +0.4 236,229 - +2.5 236,316 + +0.0 236,316 + +0.5 236,329 + +0.2 236,329 + +0.5 236,329 + -1.5 236,329 + +0.3 236,329 + -0.1 236,329 + +1.5 236,418 + EX31_RS01085 0.18 +1.9 236,433 + EX31_RS01085 0.19 -0.1 236,433 + EX31_RS01085 0.19 +0.0 236,527 + EX31_RS01085 0.32 -1.8 236,527 + EX31_RS01085 0.32 +0.9 236,528 - EX31_RS01085 0.32 +0.0 236,641 - EX31_RS01085 0.46 +0.3 236,965 - EX31_RS01085 0.88 -1.4 237,012 + +0.1 237,012 + +1.1 237,088 - +0.5 237,093 + -0.2 237,093 + -0.4 237,093 + -0.9 237,093 + +1.2 237,093 + -2.6 237,093 + +2.5 237,093 + -2.3 237,093 + -0.7 237,093 + +0.7 237,093 + -0.7 237,093 + +0.5 237,093 + +1.3 237,093 + -0.4 237,093 + +0.0 237,093 + -0.1 237,093 + +0.7 237,093 + -0.4 237,093 + -0.1 237,093 + -1.7 237,093 + -0.0 237,094 - -1.1 237,095 + -0.1 237,095 + +0.6 237,096 - +1.9 237,276 - EX31_RS01090 0.26 +0.9 237,379 + EX31_RS01090 0.38 +1.3 237,434 + EX31_RS01090 0.44 +0.9 237,463 + EX31_RS01090 0.47 -0.9 237,476 + EX31_RS01090 0.49 -0.5 237,509 + EX31_RS01090 0.53 -0.1 237,602 + EX31_RS01090 0.64 -1.0 237,631 + EX31_RS01090 0.67 +0.9 237,691 - EX31_RS01090 0.74 -0.5 237,778 - EX31_RS01090 0.84 -1.8 237,890 + +0.5 237,956 - -0.2 238,080 + EX31_RS01095 0.20 +0.9 238,091 + EX31_RS01095 0.21 +1.3 238,219 + EX31_RS01095 0.36 -0.4 238,434 + EX31_RS01095 0.62 -0.1 238,448 + EX31_RS01095 0.63 +0.9 238,449 - EX31_RS01095 0.63 +0.7 238,462 + EX31_RS01095 0.65 +0.9 238,462 + EX31_RS01095 0.65 +0.9 238,462 + EX31_RS01095 0.65 -0.2 238,462 + EX31_RS01095 0.65 +0.9 238,462 + EX31_RS01095 0.65 +1.1 238,462 + EX31_RS01095 0.65 -1.7 238,463 - EX31_RS01095 0.65 +0.9 238,640 + EX31_RS01095 0.86 +0.2 238,640 + EX31_RS01095 0.86 -0.1 238,640 + EX31_RS01095 0.86 +0.4 238,640 + EX31_RS01095 0.86 -0.1 238,641 - EX31_RS01095 0.86 -0.4 238,641 - EX31_RS01095 0.86 -1.3 238,641 - EX31_RS01095 0.86 +1.9 238,641 - EX31_RS01095 0.86 -0.6 238,642 + EX31_RS01095 0.86 -1.3 238,642 + EX31_RS01095 0.86 -0.8 238,756 + +1.1 238,760 + -0.4 238,760 + +0.4 238,760 + +1.7 238,841 - +1.7 238,884 + +1.6 238,884 + -0.7 238,885 - +0.3 238,901 + +0.9 238,901 + +0.7 238,901 + +1.9 238,901 + +0.1
Or see this region's nucleotide sequence