Strain Fitness in Rahnella sp. WP5 around EX31_RS00855

Experiment: L-Serine (N); RCH2 media; shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS00850 and EX31_RS00855 are separated by 121 nucleotidesEX31_RS00855 and EX31_RS00860 are separated by 2 nucleotidesEX31_RS00860 and EX31_RS00865 are separated by 136 nucleotides EX31_RS00850: EX31_RS00850 - GntR family transcriptional regulator, at 184,909 to 185,610 _RS00850 EX31_RS00855: EX31_RS00855 - amidohydrolase, at 185,732 to 186,910 _RS00855 EX31_RS00860: EX31_RS00860 - pyrroline-5-carboxylate reductase, at 186,913 to 187,716 _RS00860 EX31_RS00865: EX31_RS00865 - ABC transporter permease, at 187,853 to 188,959 _RS00865 Position (kb) 185 186 187Strain fitness (log2 ratio) -2 -1 0 1 2 3at 184.829 kb on - strandat 184.829 kb on - strandat 184.832 kb on + strandat 184.993 kb on + strand, within EX31_RS00850at 184.994 kb on - strand, within EX31_RS00850at 185.167 kb on + strand, within EX31_RS00850at 185.184 kb on + strand, within EX31_RS00850at 185.186 kb on + strand, within EX31_RS00850at 185.186 kb on + strand, within EX31_RS00850at 185.186 kb on + strand, within EX31_RS00850at 185.187 kb on - strand, within EX31_RS00850at 185.345 kb on + strand, within EX31_RS00850at 185.345 kb on + strand, within EX31_RS00850at 185.352 kb on + strand, within EX31_RS00850at 185.364 kb on + strand, within EX31_RS00850at 185.365 kb on - strand, within EX31_RS00850at 185.489 kb on + strand, within EX31_RS00850at 185.489 kb on + strand, within EX31_RS00850at 185.489 kb on + strand, within EX31_RS00850at 185.489 kb on + strand, within EX31_RS00850at 185.535 kb on + strand, within EX31_RS00850at 185.625 kb on + strandat 185.625 kb on + strandat 185.652 kb on + strandat 185.652 kb on + strandat 185.653 kb on - strandat 185.665 kb on + strandat 185.729 kb on + strandat 185.729 kb on + strandat 185.729 kb on + strandat 185.730 kb on - strandat 185.730 kb on - strandat 185.730 kb on - strandat 185.731 kb on + strandat 185.731 kb on + strandat 185.731 kb on + strandat 185.732 kb on - strandat 185.732 kb on - strandat 185.756 kb on - strandat 185.858 kb on + strand, within EX31_RS00855at 185.858 kb on + strand, within EX31_RS00855at 185.858 kb on + strand, within EX31_RS00855at 185.858 kb on + strand, within EX31_RS00855at 185.858 kb on + strand, within EX31_RS00855at 185.951 kb on - strand, within EX31_RS00855at 185.961 kb on + strand, within EX31_RS00855at 186.040 kb on + strand, within EX31_RS00855at 186.104 kb on + strand, within EX31_RS00855at 186.104 kb on + strand, within EX31_RS00855at 186.104 kb on + strand, within EX31_RS00855at 186.104 kb on + strand, within EX31_RS00855at 186.104 kb on + strand, within EX31_RS00855at 186.104 kb on + strand, within EX31_RS00855at 186.150 kb on + strand, within EX31_RS00855at 186.453 kb on + strand, within EX31_RS00855at 186.453 kb on + strand, within EX31_RS00855at 186.453 kb on + strand, within EX31_RS00855at 186.454 kb on - strand, within EX31_RS00855at 186.454 kb on - strand, within EX31_RS00855at 186.454 kb on - strand, within EX31_RS00855at 186.546 kb on - strand, within EX31_RS00855at 186.696 kb on + strand, within EX31_RS00855at 186.697 kb on - strand, within EX31_RS00855at 186.697 kb on - strand, within EX31_RS00855at 186.697 kb on - strand, within EX31_RS00855at 186.697 kb on - strand, within EX31_RS00855at 186.755 kb on + strand, within EX31_RS00855at 186.756 kb on - strand, within EX31_RS00855at 186.799 kb on - strandat 186.799 kb on - strandat 186.799 kb on - strandat 186.908 kb on + strandat 186.908 kb on + strandat 186.908 kb on + strandat 186.908 kb on + strandat 186.912 kb on + strandat 186.912 kb on + strandat 186.912 kb on + strandat 186.912 kb on + strandat 186.921 kb on + strandat 186.921 kb on + strandat 186.922 kb on - strandat 186.931 kb on - strandat 187.065 kb on + strand, within EX31_RS00860at 187.065 kb on + strand, within EX31_RS00860at 187.066 kb on - strand, within EX31_RS00860at 187.068 kb on + strand, within EX31_RS00860at 187.068 kb on + strand, within EX31_RS00860at 187.068 kb on + strand, within EX31_RS00860at 187.068 kb on + strand, within EX31_RS00860at 187.134 kb on + strand, within EX31_RS00860at 187.134 kb on + strand, within EX31_RS00860at 187.134 kb on + strand, within EX31_RS00860at 187.137 kb on + strand, within EX31_RS00860at 187.137 kb on + strand, within EX31_RS00860at 187.137 kb on + strand, within EX31_RS00860at 187.137 kb on + strand, within EX31_RS00860at 187.138 kb on - strand, within EX31_RS00860at 187.198 kb on - strand, within EX31_RS00860at 187.200 kb on + strand, within EX31_RS00860at 187.201 kb on - strand, within EX31_RS00860at 187.324 kb on + strand, within EX31_RS00860at 187.324 kb on + strand, within EX31_RS00860at 187.324 kb on + strand, within EX31_RS00860at 187.324 kb on + strand, within EX31_RS00860at 187.325 kb on - strand, within EX31_RS00860at 187.325 kb on - strand, within EX31_RS00860at 187.435 kb on + strand, within EX31_RS00860at 187.435 kb on + strand, within EX31_RS00860at 187.435 kb on + strand, within EX31_RS00860at 187.435 kb on + strand, within EX31_RS00860at 187.435 kb on + strand, within EX31_RS00860at 187.435 kb on + strand, within EX31_RS00860at 187.435 kb on + strand, within EX31_RS00860at 187.435 kb on + strand, within EX31_RS00860at 187.435 kb on + strand, within EX31_RS00860at 187.435 kb on + strand, within EX31_RS00860at 187.436 kb on - strand, within EX31_RS00860at 187.444 kb on + strand, within EX31_RS00860at 187.445 kb on - strand, within EX31_RS00860at 187.548 kb on + strand, within EX31_RS00860at 187.548 kb on + strand, within EX31_RS00860at 187.548 kb on + strand, within EX31_RS00860at 187.548 kb on + strand, within EX31_RS00860at 187.587 kb on + strand, within EX31_RS00860at 187.587 kb on + strand, within EX31_RS00860at 187.587 kb on + strand, within EX31_RS00860at 187.587 kb on + strand, within EX31_RS00860at 187.587 kb on + strand, within EX31_RS00860at 187.588 kb on - strand, within EX31_RS00860at 187.588 kb on - strand, within EX31_RS00860at 187.590 kb on + strand, within EX31_RS00860at 187.590 kb on + strand, within EX31_RS00860at 187.590 kb on + strand, within EX31_RS00860at 187.590 kb on + strand, within EX31_RS00860at 187.590 kb on + strand, within EX31_RS00860at 187.591 kb on - strand, within EX31_RS00860at 187.622 kb on + strand, within EX31_RS00860at 187.622 kb on + strand, within EX31_RS00860at 187.623 kb on - strand, within EX31_RS00860at 187.623 kb on - strand, within EX31_RS00860at 187.624 kb on + strand, within EX31_RS00860at 187.624 kb on + strand, within EX31_RS00860at 187.624 kb on + strand, within EX31_RS00860at 187.683 kb on - strandat 187.689 kb on + strandat 187.741 kb on + strandat 187.741 kb on + strandat 187.742 kb on - strandat 187.742 kb on - strandat 187.789 kb on + strandat 187.789 kb on + strandat 187.789 kb on + strandat 187.790 kb on - strandat 187.790 kb on - strandat 187.790 kb on - strandat 187.790 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking
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184,829 - +1.1
184,829 - +0.4
184,832 + -0.5
184,993 + EX31_RS00850 0.12 -0.5
184,994 - EX31_RS00850 0.12 -1.6
185,167 + EX31_RS00850 0.37 -1.2
185,184 + EX31_RS00850 0.39 +0.1
185,186 + EX31_RS00850 0.39 -0.4
185,186 + EX31_RS00850 0.39 -0.1
185,186 + EX31_RS00850 0.39 +1.9
185,187 - EX31_RS00850 0.40 +2.9
185,345 + EX31_RS00850 0.62 +1.4
185,345 + EX31_RS00850 0.62 -1.1
185,352 + EX31_RS00850 0.63 +1.4
185,364 + EX31_RS00850 0.65 -0.1
185,365 - EX31_RS00850 0.65 +0.2
185,489 + EX31_RS00850 0.83 +1.1
185,489 + EX31_RS00850 0.83 +0.7
185,489 + EX31_RS00850 0.83 +0.5
185,489 + EX31_RS00850 0.83 +0.6
185,535 + EX31_RS00850 0.89 +1.6
185,625 + -0.1
185,625 + +0.6
185,652 + -0.3
185,652 + +1.5
185,653 - +0.6
185,665 + +0.8
185,729 + -0.4
185,729 + +1.2
185,729 + +0.4
185,730 - +0.9
185,730 - -0.6
185,730 - -0.1
185,731 + -0.1
185,731 + -0.1
185,731 + +0.5
185,732 - -1.6
185,732 - -0.8
185,756 - -0.6
185,858 + EX31_RS00855 0.11 -0.6
185,858 + EX31_RS00855 0.11 -0.4
185,858 + EX31_RS00855 0.11 -1.1
185,858 + EX31_RS00855 0.11 +1.4
185,858 + EX31_RS00855 0.11 -0.2
185,951 - EX31_RS00855 0.19 -1.4
185,961 + EX31_RS00855 0.19 -1.1
186,040 + EX31_RS00855 0.26 +0.9
186,104 + EX31_RS00855 0.32 +0.2
186,104 + EX31_RS00855 0.32 +1.1
186,104 + EX31_RS00855 0.32 +1.9
186,104 + EX31_RS00855 0.32 +0.8
186,104 + EX31_RS00855 0.32 -0.6
186,104 + EX31_RS00855 0.32 +0.4
186,150 + EX31_RS00855 0.35 +0.6
186,453 + EX31_RS00855 0.61 -0.6
186,453 + EX31_RS00855 0.61 +0.9
186,453 + EX31_RS00855 0.61 +0.1
186,454 - EX31_RS00855 0.61 -0.3
186,454 - EX31_RS00855 0.61 -1.9
186,454 - EX31_RS00855 0.61 -0.6
186,546 - EX31_RS00855 0.69 +0.9
186,696 + EX31_RS00855 0.82 -0.2
186,697 - EX31_RS00855 0.82 -0.3
186,697 - EX31_RS00855 0.82 -0.4
186,697 - EX31_RS00855 0.82 +0.7
186,697 - EX31_RS00855 0.82 -1.1
186,755 + EX31_RS00855 0.87 +0.1
186,756 - EX31_RS00855 0.87 -2.2
186,799 - +2.9
186,799 - +0.3
186,799 - +0.5
186,908 + +0.2
186,908 + +0.2
186,908 + -1.1
186,908 + +0.6
186,912 + -0.4
186,912 + +0.8
186,912 + +1.4
186,912 + +0.9
186,921 + -0.1
186,921 + -0.6
186,922 - +0.8
186,931 - +0.4
187,065 + EX31_RS00860 0.19 -1.2
187,065 + EX31_RS00860 0.19 -1.9
187,066 - EX31_RS00860 0.19 +0.4
187,068 + EX31_RS00860 0.19 -0.3
187,068 + EX31_RS00860 0.19 +0.4
187,068 + EX31_RS00860 0.19 +0.6
187,068 + EX31_RS00860 0.19 +1.3
187,134 + EX31_RS00860 0.27 +2.5
187,134 + EX31_RS00860 0.27 +0.1
187,134 + EX31_RS00860 0.27 +0.4
187,137 + EX31_RS00860 0.28 +0.0
187,137 + EX31_RS00860 0.28 +0.5
187,137 + EX31_RS00860 0.28 +1.7
187,137 + EX31_RS00860 0.28 -0.1
187,138 - EX31_RS00860 0.28 -0.5
187,198 - EX31_RS00860 0.35 +0.9
187,200 + EX31_RS00860 0.36 -0.2
187,201 - EX31_RS00860 0.36 +0.8
187,324 + EX31_RS00860 0.51 +0.9
187,324 + EX31_RS00860 0.51 -1.1
187,324 + EX31_RS00860 0.51 +0.9
187,324 + EX31_RS00860 0.51 -0.1
187,325 - EX31_RS00860 0.51 -0.2
187,325 - EX31_RS00860 0.51 -1.8
187,435 + EX31_RS00860 0.65 +1.3
187,435 + EX31_RS00860 0.65 -0.8
187,435 + EX31_RS00860 0.65 -0.1
187,435 + EX31_RS00860 0.65 +2.5
187,435 + EX31_RS00860 0.65 -1.2
187,435 + EX31_RS00860 0.65 +0.4
187,435 + EX31_RS00860 0.65 -0.4
187,435 + EX31_RS00860 0.65 +0.6
187,435 + EX31_RS00860 0.65 +0.7
187,435 + EX31_RS00860 0.65 +0.1
187,436 - EX31_RS00860 0.65 +0.6
187,444 + EX31_RS00860 0.66 +0.0
187,445 - EX31_RS00860 0.66 -0.2
187,548 + EX31_RS00860 0.79 -1.9
187,548 + EX31_RS00860 0.79 +0.3
187,548 + EX31_RS00860 0.79 +0.7
187,548 + EX31_RS00860 0.79 -0.1
187,587 + EX31_RS00860 0.84 +0.5
187,587 + EX31_RS00860 0.84 +0.4
187,587 + EX31_RS00860 0.84 +0.9
187,587 + EX31_RS00860 0.84 +0.9
187,587 + EX31_RS00860 0.84 +0.1
187,588 - EX31_RS00860 0.84 +1.2
187,588 - EX31_RS00860 0.84 -0.1
187,590 + EX31_RS00860 0.84 +2.3
187,590 + EX31_RS00860 0.84 +0.1
187,590 + EX31_RS00860 0.84 -1.0
187,590 + EX31_RS00860 0.84 +0.6
187,590 + EX31_RS00860 0.84 +0.0
187,591 - EX31_RS00860 0.84 +1.5
187,622 + EX31_RS00860 0.88 +0.9
187,622 + EX31_RS00860 0.88 +1.5
187,623 - EX31_RS00860 0.88 +1.9
187,623 - EX31_RS00860 0.88 +0.1
187,624 + EX31_RS00860 0.88 +2.9
187,624 + EX31_RS00860 0.88 -0.5
187,624 + EX31_RS00860 0.88 +0.1
187,683 - -0.6
187,689 + -0.1
187,741 + -0.1
187,741 + -1.2
187,742 - +0.4
187,742 - +0.4
187,789 + +0.2
187,789 + +0.5
187,789 + +0.6
187,790 - -1.2
187,790 - -0.6
187,790 - -1.8
187,790 - -0.8

Or see this region's nucleotide sequence