Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08295 and MMJJ_RS08300 are separated by 130 nucleotides MMJJ_RS08300 and MMJJ_RS08305 are separated by 140 nucleotides
MMJJ_RS08295: MMJJ_RS08295 - iron ABC transporter substrate-binding protein, at 1,529,125 to 1,530,249
_RS08295
MMJJ_RS08300: MMJJ_RS08300 - hypothetical protein, at 1,530,380 to 1,531,462
_RS08300
MMJJ_RS08305: MMJJ_RS08305 - peroxiredoxin, at 1,531,603 to 1,532,256
_RS08305
Position (kb)
1530
1531
1532 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1529.381 kb on - strand, within MMJJ_RS08295 at 1529.382 kb on + strand, within MMJJ_RS08295 at 1529.399 kb on - strand, within MMJJ_RS08295 at 1529.420 kb on - strand, within MMJJ_RS08295 at 1529.421 kb on + strand, within MMJJ_RS08295 at 1529.549 kb on - strand, within MMJJ_RS08295 at 1529.553 kb on - strand, within MMJJ_RS08295 at 1529.602 kb on - strand, within MMJJ_RS08295 at 1529.606 kb on - strand, within MMJJ_RS08295 at 1529.606 kb on - strand, within MMJJ_RS08295 at 1529.606 kb on - strand, within MMJJ_RS08295 at 1529.606 kb on - strand, within MMJJ_RS08295 at 1529.606 kb on - strand, within MMJJ_RS08295 at 1529.606 kb on - strand, within MMJJ_RS08295 at 1529.650 kb on - strand, within MMJJ_RS08295 at 1529.729 kb on + strand, within MMJJ_RS08295 at 1529.769 kb on - strand, within MMJJ_RS08295 at 1529.769 kb on - strand, within MMJJ_RS08295 at 1529.770 kb on + strand, within MMJJ_RS08295 at 1529.770 kb on + strand, within MMJJ_RS08295 at 1529.781 kb on + strand, within MMJJ_RS08295 at 1529.786 kb on + strand, within MMJJ_RS08295 at 1529.788 kb on - strand, within MMJJ_RS08295 at 1529.789 kb on + strand, within MMJJ_RS08295 at 1529.791 kb on - strand, within MMJJ_RS08295 at 1529.791 kb on - strand, within MMJJ_RS08295 at 1529.792 kb on + strand, within MMJJ_RS08295 at 1529.792 kb on + strand, within MMJJ_RS08295 at 1529.792 kb on + strand, within MMJJ_RS08295 at 1529.792 kb on + strand, within MMJJ_RS08295 at 1529.792 kb on + strand, within MMJJ_RS08295 at 1529.807 kb on - strand, within MMJJ_RS08295 at 1529.833 kb on + strand, within MMJJ_RS08295 at 1529.833 kb on + strand, within MMJJ_RS08295 at 1529.846 kb on - strand, within MMJJ_RS08295 at 1529.846 kb on - strand, within MMJJ_RS08295 at 1529.846 kb on - strand, within MMJJ_RS08295 at 1529.846 kb on - strand, within MMJJ_RS08295 at 1529.846 kb on - strand, within MMJJ_RS08295 at 1529.846 kb on - strand, within MMJJ_RS08295 at 1529.846 kb on - strand, within MMJJ_RS08295 at 1529.847 kb on + strand, within MMJJ_RS08295 at 1529.847 kb on + strand, within MMJJ_RS08295 at 1529.847 kb on + strand, within MMJJ_RS08295 at 1529.850 kb on - strand, within MMJJ_RS08295 at 1529.851 kb on + strand, within MMJJ_RS08295 at 1529.854 kb on - strand at 1529.854 kb on - strand, within MMJJ_RS08295 at 1529.855 kb on + strand, within MMJJ_RS08295 at 1529.855 kb on + strand, within MMJJ_RS08295 at 1529.855 kb on + strand, within MMJJ_RS08295 at 1529.855 kb on + strand, within MMJJ_RS08295 at 1529.858 kb on + strand, within MMJJ_RS08295 at 1529.859 kb on - strand, within MMJJ_RS08295 at 1529.859 kb on - strand, within MMJJ_RS08295 at 1529.860 kb on + strand, within MMJJ_RS08295 at 1529.878 kb on - strand, within MMJJ_RS08295 at 1529.878 kb on - strand, within MMJJ_RS08295 at 1529.878 kb on - strand, within MMJJ_RS08295 at 1529.878 kb on - strand, within MMJJ_RS08295 at 1529.879 kb on + strand, within MMJJ_RS08295 at 1529.879 kb on + strand, within MMJJ_RS08295 at 1529.879 kb on + strand, within MMJJ_RS08295 at 1529.879 kb on + strand, within MMJJ_RS08295 at 1529.938 kb on + strand, within MMJJ_RS08295 at 1529.992 kb on + strand, within MMJJ_RS08295 at 1530.005 kb on + strand, within MMJJ_RS08295 at 1530.045 kb on - strand, within MMJJ_RS08295 at 1530.049 kb on - strand, within MMJJ_RS08295 at 1530.177 kb on - strand at 1530.230 kb on + strand at 1530.341 kb on - strand at 1530.341 kb on - strand at 1530.381 kb on - strand at 1530.441 kb on + strand at 1530.467 kb on - strand at 1530.501 kb on - strand, within MMJJ_RS08300 at 1530.501 kb on - strand, within MMJJ_RS08300 at 1530.515 kb on + strand, within MMJJ_RS08300 at 1530.533 kb on + strand, within MMJJ_RS08300 at 1530.535 kb on + strand, within MMJJ_RS08300 at 1530.539 kb on - strand, within MMJJ_RS08300 at 1530.539 kb on - strand, within MMJJ_RS08300 at 1530.540 kb on + strand, within MMJJ_RS08300 at 1530.540 kb on + strand, within MMJJ_RS08300 at 1530.540 kb on + strand, within MMJJ_RS08300 at 1530.541 kb on - strand, within MMJJ_RS08300 at 1530.541 kb on - strand, within MMJJ_RS08300 at 1530.542 kb on + strand, within MMJJ_RS08300 at 1530.542 kb on + strand at 1530.542 kb on + strand, within MMJJ_RS08300 at 1530.542 kb on + strand, within MMJJ_RS08300 at 1530.593 kb on - strand, within MMJJ_RS08300 at 1530.647 kb on - strand, within MMJJ_RS08300 at 1530.648 kb on + strand, within MMJJ_RS08300 at 1530.648 kb on + strand, within MMJJ_RS08300 at 1530.659 kb on + strand, within MMJJ_RS08300 at 1530.672 kb on - strand, within MMJJ_RS08300 at 1530.778 kb on - strand, within MMJJ_RS08300 at 1530.830 kb on + strand, within MMJJ_RS08300 at 1530.961 kb on - strand, within MMJJ_RS08300 at 1530.961 kb on - strand, within MMJJ_RS08300 at 1530.961 kb on - strand, within MMJJ_RS08300 at 1530.962 kb on + strand, within MMJJ_RS08300 at 1530.964 kb on + strand, within MMJJ_RS08300 at 1530.964 kb on + strand, within MMJJ_RS08300 at 1531.016 kb on + strand, within MMJJ_RS08300 at 1531.020 kb on + strand, within MMJJ_RS08300 at 1531.140 kb on + strand, within MMJJ_RS08300 at 1531.140 kb on + strand, within MMJJ_RS08300 at 1531.140 kb on + strand, within MMJJ_RS08300 at 1531.158 kb on - strand, within MMJJ_RS08300 at 1531.163 kb on + strand, within MMJJ_RS08300 at 1531.185 kb on - strand, within MMJJ_RS08300 at 1531.186 kb on + strand, within MMJJ_RS08300 at 1531.186 kb on + strand, within MMJJ_RS08300 at 1531.220 kb on + strand, within MMJJ_RS08300 at 1531.260 kb on + strand, within MMJJ_RS08300 at 1531.289 kb on - strand, within MMJJ_RS08300 at 1531.321 kb on + strand, within MMJJ_RS08300 at 1531.321 kb on + strand, within MMJJ_RS08300 at 1531.329 kb on + strand, within MMJJ_RS08300 at 1531.356 kb on + strand at 1531.380 kb on - strand at 1531.381 kb on + strand at 1531.492 kb on + strand at 1531.498 kb on + strand at 1531.498 kb on + strand at 1531.512 kb on - strand at 1531.513 kb on + strand at 1531.513 kb on + strand at 1531.513 kb on + strand at 1531.513 kb on + strand at 1531.514 kb on - strand at 1531.516 kb on - strand at 1531.583 kb on + strand at 1531.604 kb on - strand at 1531.621 kb on + strand at 1531.623 kb on + strand at 1531.767 kb on + strand, within MMJJ_RS08305 at 1531.773 kb on - strand, within MMJJ_RS08305 at 1531.796 kb on + strand, within MMJJ_RS08305 at 1531.879 kb on - strand, within MMJJ_RS08305 at 1531.978 kb on - strand, within MMJJ_RS08305 at 1531.979 kb on + strand, within MMJJ_RS08305 at 1531.990 kb on - strand, within MMJJ_RS08305 at 1532.020 kb on - strand, within MMJJ_RS08305 at 1532.021 kb on + strand, within MMJJ_RS08305 at 1532.021 kb on + strand, within MMJJ_RS08305 at 1532.044 kb on + strand, within MMJJ_RS08305 at 1532.056 kb on - strand, within MMJJ_RS08305 at 1532.115 kb on - strand, within MMJJ_RS08305 at 1532.118 kb on - strand, within MMJJ_RS08305 at 1532.152 kb on - strand, within MMJJ_RS08305 at 1532.180 kb on - strand, within MMJJ_RS08305 at 1532.181 kb on + strand, within MMJJ_RS08305 at 1532.215 kb on - strand at 1532.215 kb on - strand at 1532.216 kb on + strand at 1532.218 kb on + strand at 1532.218 kb on + strand at 1532.248 kb on - strand at 1532.249 kb on + strand at 1532.260 kb on + strand at 1532.283 kb on - strand at 1532.283 kb on - strand at 1532.283 kb on - strand at 1532.284 kb on + strand at 1532.284 kb on + strand at 1532.284 kb on + strand at 1532.284 kb on + strand at 1532.284 kb on + strand at 1532.287 kb on - strand at 1532.288 kb on + strand at 1532.289 kb on - strand at 1532.290 kb on + strand at 1532.290 kb on + strand at 1532.290 kb on + strand at 1532.310 kb on - strand at 1532.311 kb on + strand at 1532.311 kb on + strand at 1532.311 kb on + strand at 1532.312 kb on - strand at 1532.313 kb on + strand at 1532.314 kb on - strand at 1532.314 kb on - strand at 1532.315 kb on + strand at 1532.315 kb on + strand at 1532.398 kb on - strand at 1532.398 kb on - strand at 1532.398 kb on - strand at 1532.398 kb on - strand at 1532.398 kb on - strand at 1532.398 kb on - strand at 1532.399 kb on + strand at 1532.399 kb on + strand at 1532.399 kb on + strand at 1532.400 kb on - strand at 1532.400 kb on - strand at 1532.400 kb on - strand at 1532.401 kb on + strand at 1532.401 kb on + strand at 1532.401 kb on + strand at 1532.401 kb on + strand at 1532.402 kb on - strand at 1532.402 kb on - strand at 1532.403 kb on + strand at 1532.403 kb on + strand at 1532.403 kb on + strand at 1532.403 kb on + strand at 1532.403 kb on + strand at 1532.413 kb on - strand at 1532.427 kb on + strand at 1532.436 kb on - strand at 1532.449 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE remove 1,529,381 - MMJJ_RS08295 0.23 +0.3 1,529,382 + MMJJ_RS08295 0.23 -0.7 1,529,399 - MMJJ_RS08295 0.24 +0.7 1,529,420 - MMJJ_RS08295 0.26 +1.8 1,529,421 + MMJJ_RS08295 0.26 +0.2 1,529,549 - MMJJ_RS08295 0.38 +0.3 1,529,553 - MMJJ_RS08295 0.38 +0.2 1,529,602 - MMJJ_RS08295 0.42 -1.3 1,529,606 - MMJJ_RS08295 0.43 +1.5 1,529,606 - MMJJ_RS08295 0.43 -0.6 1,529,606 - MMJJ_RS08295 0.43 -0.7 1,529,606 - MMJJ_RS08295 0.43 +0.4 1,529,606 - MMJJ_RS08295 0.43 +0.2 1,529,606 - MMJJ_RS08295 0.43 +1.7 1,529,650 - MMJJ_RS08295 0.47 -0.3 1,529,729 + MMJJ_RS08295 0.54 -0.2 1,529,769 - MMJJ_RS08295 0.57 +0.7 1,529,769 - MMJJ_RS08295 0.57 +0.2 1,529,770 + MMJJ_RS08295 0.57 +0.3 1,529,770 + MMJJ_RS08295 0.57 -0.0 1,529,781 + MMJJ_RS08295 0.58 +0.5 1,529,786 + MMJJ_RS08295 0.59 -0.9 1,529,788 - MMJJ_RS08295 0.59 +1.8 1,529,789 + MMJJ_RS08295 0.59 -1.7 1,529,791 - MMJJ_RS08295 0.59 -0.1 1,529,791 - MMJJ_RS08295 0.59 +0.8 1,529,792 + MMJJ_RS08295 0.59 +0.0 1,529,792 + MMJJ_RS08295 0.59 -1.1 1,529,792 + MMJJ_RS08295 0.59 +0.0 1,529,792 + MMJJ_RS08295 0.59 -0.6 1,529,792 + MMJJ_RS08295 0.59 -0.5 1,529,807 - MMJJ_RS08295 0.61 +0.3 1,529,833 + MMJJ_RS08295 0.63 +0.5 1,529,833 + MMJJ_RS08295 0.63 -0.9 1,529,846 - MMJJ_RS08295 0.64 +0.7 1,529,846 - MMJJ_RS08295 0.64 +0.3 1,529,846 - MMJJ_RS08295 0.64 +0.2 1,529,846 - MMJJ_RS08295 0.64 -0.4 1,529,846 - MMJJ_RS08295 0.64 -0.4 1,529,846 - MMJJ_RS08295 0.64 +1.6 1,529,846 - MMJJ_RS08295 0.64 -3.7 1,529,847 + MMJJ_RS08295 0.64 +2.2 1,529,847 + MMJJ_RS08295 0.64 -1.3 1,529,847 + MMJJ_RS08295 0.64 +1.2 1,529,850 - MMJJ_RS08295 0.64 -0.9 1,529,851 + MMJJ_RS08295 0.65 -0.1 1,529,854 - -1.3 1,529,854 - MMJJ_RS08295 0.65 +0.2 1,529,855 + MMJJ_RS08295 0.65 +0.2 1,529,855 + MMJJ_RS08295 0.65 +0.7 1,529,855 + MMJJ_RS08295 0.65 +1.4 1,529,855 + MMJJ_RS08295 0.65 +1.0 1,529,858 + MMJJ_RS08295 0.65 -0.0 1,529,859 - MMJJ_RS08295 0.65 +0.8 1,529,859 - MMJJ_RS08295 0.65 +1.6 1,529,860 + MMJJ_RS08295 0.65 +0.5 1,529,878 - MMJJ_RS08295 0.67 +2.3 1,529,878 - MMJJ_RS08295 0.67 -0.2 1,529,878 - MMJJ_RS08295 0.67 +0.2 1,529,878 - MMJJ_RS08295 0.67 +0.0 1,529,879 + MMJJ_RS08295 0.67 -0.5 1,529,879 + MMJJ_RS08295 0.67 +1.8 1,529,879 + MMJJ_RS08295 0.67 +0.0 1,529,879 + MMJJ_RS08295 0.67 +0.4 1,529,938 + MMJJ_RS08295 0.72 -0.1 1,529,992 + MMJJ_RS08295 0.77 -0.8 1,530,005 + MMJJ_RS08295 0.78 -0.5 1,530,045 - MMJJ_RS08295 0.82 +0.5 1,530,049 - MMJJ_RS08295 0.82 -0.1 1,530,177 - -0.5 1,530,230 + -0.3 1,530,341 - -0.8 1,530,341 - -0.1 1,530,381 - +0.7 1,530,441 + +0.2 1,530,467 - +2.1 1,530,501 - MMJJ_RS08300 0.11 +0.1 1,530,501 - MMJJ_RS08300 0.11 -1.5 1,530,515 + MMJJ_RS08300 0.12 +1.3 1,530,533 + MMJJ_RS08300 0.14 +0.1 1,530,535 + MMJJ_RS08300 0.14 -0.2 1,530,539 - MMJJ_RS08300 0.15 -0.1 1,530,539 - MMJJ_RS08300 0.15 -0.6 1,530,540 + MMJJ_RS08300 0.15 -0.7 1,530,540 + MMJJ_RS08300 0.15 +0.1 1,530,540 + MMJJ_RS08300 0.15 -0.0 1,530,541 - MMJJ_RS08300 0.15 +0.0 1,530,541 - MMJJ_RS08300 0.15 -0.8 1,530,542 + MMJJ_RS08300 0.15 -0.3 1,530,542 + -0.3 1,530,542 + MMJJ_RS08300 0.15 +0.1 1,530,542 + MMJJ_RS08300 0.15 +0.1 1,530,593 - MMJJ_RS08300 0.20 +0.4 1,530,647 - MMJJ_RS08300 0.25 +0.3 1,530,648 + MMJJ_RS08300 0.25 +0.4 1,530,648 + MMJJ_RS08300 0.25 +0.1 1,530,659 + MMJJ_RS08300 0.26 -0.7 1,530,672 - MMJJ_RS08300 0.27 -0.4 1,530,778 - MMJJ_RS08300 0.37 +0.0 1,530,830 + MMJJ_RS08300 0.42 -0.9 1,530,961 - MMJJ_RS08300 0.54 +0.4 1,530,961 - MMJJ_RS08300 0.54 +0.7 1,530,961 - MMJJ_RS08300 0.54 +0.8 1,530,962 + MMJJ_RS08300 0.54 -0.5 1,530,964 + MMJJ_RS08300 0.54 -0.6 1,530,964 + MMJJ_RS08300 0.54 -0.3 1,531,016 + MMJJ_RS08300 0.59 -0.0 1,531,020 + MMJJ_RS08300 0.59 -0.7 1,531,140 + MMJJ_RS08300 0.70 -0.3 1,531,140 + MMJJ_RS08300 0.70 -0.7 1,531,140 + MMJJ_RS08300 0.70 +0.9 1,531,158 - MMJJ_RS08300 0.72 +0.5 1,531,163 + MMJJ_RS08300 0.72 -1.1 1,531,185 - MMJJ_RS08300 0.74 +0.1 1,531,186 + MMJJ_RS08300 0.74 +0.5 1,531,186 + MMJJ_RS08300 0.74 -0.0 1,531,220 + MMJJ_RS08300 0.78 -0.3 1,531,260 + MMJJ_RS08300 0.81 -2.9 1,531,289 - MMJJ_RS08300 0.84 +0.2 1,531,321 + MMJJ_RS08300 0.87 -0.4 1,531,321 + MMJJ_RS08300 0.87 -0.3 1,531,329 + MMJJ_RS08300 0.88 -0.1 1,531,356 + -0.5 1,531,380 - +0.1 1,531,381 + +0.3 1,531,492 + -0.3 1,531,498 + -0.5 1,531,498 + +0.7 1,531,512 - +0.1 1,531,513 + -0.3 1,531,513 + -0.1 1,531,513 + +1.8 1,531,513 + +0.2 1,531,514 - -0.2 1,531,516 - -0.2 1,531,583 + +1.8 1,531,604 - -0.4 1,531,621 + +1.4 1,531,623 + -0.4 1,531,767 + MMJJ_RS08305 0.25 -0.3 1,531,773 - MMJJ_RS08305 0.26 +0.6 1,531,796 + MMJJ_RS08305 0.30 +0.7 1,531,879 - MMJJ_RS08305 0.42 +0.8 1,531,978 - MMJJ_RS08305 0.57 +0.2 1,531,979 + MMJJ_RS08305 0.57 +0.7 1,531,990 - MMJJ_RS08305 0.59 -0.2 1,532,020 - MMJJ_RS08305 0.64 +0.4 1,532,021 + MMJJ_RS08305 0.64 -0.8 1,532,021 + MMJJ_RS08305 0.64 +0.7 1,532,044 + MMJJ_RS08305 0.67 -0.3 1,532,056 - MMJJ_RS08305 0.69 +1.8 1,532,115 - MMJJ_RS08305 0.78 -0.4 1,532,118 - MMJJ_RS08305 0.79 +0.1 1,532,152 - MMJJ_RS08305 0.84 +0.0 1,532,180 - MMJJ_RS08305 0.88 -0.3 1,532,181 + MMJJ_RS08305 0.88 -0.0 1,532,215 - -0.8 1,532,215 - -0.5 1,532,216 + +0.4 1,532,218 + +0.1 1,532,218 + +0.1 1,532,248 - -0.5 1,532,249 + +0.4 1,532,260 + +0.3 1,532,283 - +1.7 1,532,283 - -0.2 1,532,283 - +0.3 1,532,284 + -1.0 1,532,284 + +0.0 1,532,284 + +1.5 1,532,284 + -0.1 1,532,284 + +0.7 1,532,287 - +0.6 1,532,288 + -0.3 1,532,289 - -0.4 1,532,290 + -5.1 1,532,290 + +0.2 1,532,290 + -0.1 1,532,310 - +0.3 1,532,311 + -0.2 1,532,311 + +0.4 1,532,311 + -0.1 1,532,312 - -0.6 1,532,313 + -0.2 1,532,314 - -0.2 1,532,314 - +2.5 1,532,315 + -0.5 1,532,315 + +0.9 1,532,398 - -0.1 1,532,398 - -0.0 1,532,398 - -0.8 1,532,398 - -0.1 1,532,398 - -0.2 1,532,398 - +0.4 1,532,399 + +0.3 1,532,399 + -0.0 1,532,399 + +2.9 1,532,400 - -1.0 1,532,400 - -0.1 1,532,400 - -0.2 1,532,401 + +1.3 1,532,401 + +1.6 1,532,401 + +0.4 1,532,401 + +0.5 1,532,402 - -1.3 1,532,402 - +0.2 1,532,403 + +0.1 1,532,403 + +1.7 1,532,403 + +2.1 1,532,403 + +0.4 1,532,403 + -0.5 1,532,413 - -0.0 1,532,427 + +0.5 1,532,436 - -1.4 1,532,449 + +0.6
Or see this region's nucleotide sequence