Experiment: R2A with Nickel (II) chloride 0.3125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS04855 and LRK54_RS04860 are separated by 159 nucleotides LRK54_RS04860 and LRK54_RS04865 are separated by 11 nucleotides LRK54_RS04865 and LRK54_RS04870 are separated by 41 nucleotides LRK54_RS04870 and LRK54_RS04875 are separated by 249 nucleotides
LRK54_RS04855: LRK54_RS04855 - 7-cyano-7-deazaguanine synthase QueC, at 1,030,988 to 1,031,674
_RS04855
LRK54_RS04860: LRK54_RS04860 - 7-carboxy-7-deazaguanine synthase QueE, at 1,031,834 to 1,032,526
_RS04860
LRK54_RS04865: LRK54_RS04865 - tol-pal system protein YbgF, at 1,032,538 to 1,033,359
_RS04865
LRK54_RS04870: LRK54_RS04870 - peptidoglycan-associated lipoprotein Pal, at 1,033,401 to 1,033,907
_RS04870
LRK54_RS04875: LRK54_RS04875 - Tol-Pal system beta propeller repeat protein TolB, at 1,034,157 to 1,035,476
_RS04875
Position (kb)
1032
1033
1034 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1031.556 kb on + strand, within LRK54_RS04855 at 1031.557 kb on - strand, within LRK54_RS04855 at 1031.583 kb on + strand, within LRK54_RS04855 at 1031.583 kb on + strand, within LRK54_RS04855 at 1031.583 kb on + strand, within LRK54_RS04855 at 1031.612 kb on - strand at 1031.612 kb on - strand at 1031.694 kb on - strand at 1031.694 kb on - strand at 1031.746 kb on - strand at 1031.746 kb on - strand at 1031.835 kb on + strand at 1031.835 kb on + strand at 1031.835 kb on + strand at 1031.836 kb on - strand at 1031.836 kb on - strand at 1031.836 kb on - strand at 1031.836 kb on - strand at 1031.836 kb on - strand at 1031.836 kb on - strand at 1031.836 kb on - strand at 1032.018 kb on + strand, within LRK54_RS04860 at 1032.018 kb on + strand, within LRK54_RS04860 at 1032.018 kb on + strand, within LRK54_RS04860 at 1032.018 kb on + strand, within LRK54_RS04860 at 1032.198 kb on + strand, within LRK54_RS04860 at 1032.198 kb on + strand, within LRK54_RS04860 at 1032.198 kb on + strand, within LRK54_RS04860 at 1032.311 kb on - strand, within LRK54_RS04860 at 1032.311 kb on - strand, within LRK54_RS04860 at 1032.339 kb on + strand, within LRK54_RS04860 at 1032.339 kb on + strand, within LRK54_RS04860 at 1032.339 kb on + strand, within LRK54_RS04860 at 1032.340 kb on - strand, within LRK54_RS04860 at 1032.340 kb on - strand, within LRK54_RS04860 at 1032.340 kb on - strand, within LRK54_RS04860 at 1032.340 kb on - strand, within LRK54_RS04860 at 1032.340 kb on - strand, within LRK54_RS04860 at 1032.340 kb on - strand, within LRK54_RS04860 at 1032.340 kb on - strand, within LRK54_RS04860 at 1032.348 kb on + strand, within LRK54_RS04860 at 1032.349 kb on - strand, within LRK54_RS04860 at 1032.349 kb on - strand, within LRK54_RS04860 at 1032.349 kb on - strand, within LRK54_RS04860 at 1032.531 kb on + strand at 1032.532 kb on - strand at 1032.535 kb on + strand at 1032.535 kb on + strand at 1032.535 kb on + strand at 1032.535 kb on + strand at 1032.535 kb on + strand at 1032.535 kb on + strand at 1032.535 kb on + strand at 1032.535 kb on + strand at 1032.535 kb on + strand at 1032.536 kb on - strand at 1032.536 kb on - strand at 1032.536 kb on - strand at 1032.536 kb on - strand at 1032.536 kb on - strand at 1032.536 kb on - strand at 1032.536 kb on - strand at 1032.536 kb on - strand at 1032.536 kb on - strand at 1032.536 kb on - strand at 1032.536 kb on - strand at 1032.536 kb on - strand at 1032.608 kb on + strand at 1032.608 kb on + strand at 1032.608 kb on + strand at 1032.608 kb on + strand at 1032.608 kb on + strand at 1032.608 kb on + strand at 1032.609 kb on - strand at 1032.609 kb on - strand at 1032.609 kb on - strand at 1032.609 kb on - strand at 1032.609 kb on - strand at 1032.675 kb on - strand, within LRK54_RS04865 at 1032.761 kb on + strand, within LRK54_RS04865 at 1032.761 kb on + strand, within LRK54_RS04865 at 1032.762 kb on - strand, within LRK54_RS04865 at 1032.762 kb on - strand, within LRK54_RS04865 at 1032.776 kb on + strand, within LRK54_RS04865 at 1032.776 kb on + strand, within LRK54_RS04865 at 1032.776 kb on + strand, within LRK54_RS04865 at 1032.776 kb on + strand, within LRK54_RS04865 at 1032.776 kb on + strand, within LRK54_RS04865 at 1032.776 kb on + strand, within LRK54_RS04865 at 1032.776 kb on + strand, within LRK54_RS04865 at 1032.776 kb on + strand, within LRK54_RS04865 at 1032.776 kb on + strand, within LRK54_RS04865 at 1032.777 kb on - strand, within LRK54_RS04865 at 1032.777 kb on - strand, within LRK54_RS04865 at 1032.777 kb on - strand, within LRK54_RS04865 at 1032.777 kb on - strand, within LRK54_RS04865 at 1032.777 kb on - strand, within LRK54_RS04865 at 1032.777 kb on - strand, within LRK54_RS04865 at 1032.777 kb on - strand, within LRK54_RS04865 at 1032.777 kb on - strand, within LRK54_RS04865 at 1032.777 kb on - strand, within LRK54_RS04865 at 1032.777 kb on - strand, within LRK54_RS04865 at 1032.777 kb on - strand, within LRK54_RS04865 at 1032.777 kb on - strand, within LRK54_RS04865 at 1032.777 kb on - strand, within LRK54_RS04865 at 1032.777 kb on - strand, within LRK54_RS04865 at 1032.777 kb on - strand, within LRK54_RS04865 at 1032.777 kb on - strand, within LRK54_RS04865 at 1032.779 kb on + strand, within LRK54_RS04865 at 1032.779 kb on + strand, within LRK54_RS04865 at 1032.779 kb on + strand, within LRK54_RS04865 at 1032.779 kb on + strand, within LRK54_RS04865 at 1032.780 kb on - strand, within LRK54_RS04865 at 1032.780 kb on - strand, within LRK54_RS04865 at 1032.780 kb on - strand, within LRK54_RS04865 at 1032.780 kb on - strand, within LRK54_RS04865 at 1032.780 kb on - strand, within LRK54_RS04865 at 1032.780 kb on - strand, within LRK54_RS04865 at 1032.780 kb on - strand, within LRK54_RS04865 at 1032.780 kb on - strand, within LRK54_RS04865 at 1032.780 kb on - strand, within LRK54_RS04865 at 1032.780 kb on - strand, within LRK54_RS04865 at 1032.780 kb on - strand, within LRK54_RS04865 at 1032.780 kb on - strand, within LRK54_RS04865 at 1032.780 kb on - strand, within LRK54_RS04865 at 1032.780 kb on - strand, within LRK54_RS04865 at 1032.780 kb on - strand, within LRK54_RS04865 at 1032.797 kb on + strand, within LRK54_RS04865 at 1032.798 kb on - strand, within LRK54_RS04865 at 1032.798 kb on - strand, within LRK54_RS04865 at 1032.798 kb on - strand, within LRK54_RS04865 at 1032.798 kb on - strand, within LRK54_RS04865 at 1032.798 kb on - strand, within LRK54_RS04865 at 1032.830 kb on + strand, within LRK54_RS04865 at 1032.831 kb on - strand, within LRK54_RS04865 at 1032.831 kb on - strand, within LRK54_RS04865 at 1032.831 kb on - strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.872 kb on + strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.873 kb on - strand, within LRK54_RS04865 at 1032.874 kb on - strand, within LRK54_RS04865 at 1032.908 kb on + strand, within LRK54_RS04865 at 1032.908 kb on + strand, within LRK54_RS04865 at 1032.909 kb on - strand, within LRK54_RS04865 at 1033.118 kb on + strand, within LRK54_RS04865 at 1033.118 kb on + strand, within LRK54_RS04865 at 1033.118 kb on + strand, within LRK54_RS04865 at 1033.118 kb on + strand, within LRK54_RS04865 at 1033.119 kb on - strand, within LRK54_RS04865 at 1033.119 kb on - strand, within LRK54_RS04865 at 1033.119 kb on - strand, within LRK54_RS04865 at 1033.119 kb on - strand, within LRK54_RS04865 at 1033.119 kb on - strand, within LRK54_RS04865 at 1033.403 kb on - strand at 1033.990 kb on - strand at 1033.990 kb on - strand at 1034.016 kb on - strand at 1034.016 kb on - strand at 1034.016 kb on - strand at 1034.016 kb on - strand at 1034.016 kb on - strand at 1034.016 kb on - strand at 1034.016 kb on - strand at 1034.016 kb on - strand at 1034.016 kb on - strand at 1034.016 kb on - strand at 1034.016 kb on - strand at 1034.016 kb on - strand at 1034.016 kb on - strand at 1034.023 kb on - strand at 1034.023 kb on - strand at 1034.023 kb on - strand at 1034.023 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.3125 mM remove 1,031,556 + LRK54_RS04855 0.83 -1.7 1,031,557 - LRK54_RS04855 0.83 -1.1 1,031,583 + LRK54_RS04855 0.87 -1.1 1,031,583 + LRK54_RS04855 0.87 -1.8 1,031,583 + LRK54_RS04855 0.87 -2.0 1,031,612 - +0.9 1,031,612 - -1.0 1,031,694 - -0.9 1,031,694 - +0.5 1,031,746 - +1.6 1,031,746 - +0.0 1,031,835 + -0.1 1,031,835 + -0.0 1,031,835 + +0.9 1,031,836 - -0.2 1,031,836 - -1.0 1,031,836 - +0.4 1,031,836 - -1.0 1,031,836 - +0.8 1,031,836 - +1.3 1,031,836 - -0.6 1,032,018 + LRK54_RS04860 0.27 -0.3 1,032,018 + LRK54_RS04860 0.27 +1.0 1,032,018 + LRK54_RS04860 0.27 -0.8 1,032,018 + LRK54_RS04860 0.27 +0.1 1,032,198 + LRK54_RS04860 0.53 -0.4 1,032,198 + LRK54_RS04860 0.53 -0.9 1,032,198 + LRK54_RS04860 0.53 -3.2 1,032,311 - LRK54_RS04860 0.69 -1.5 1,032,311 - LRK54_RS04860 0.69 -1.6 1,032,339 + LRK54_RS04860 0.73 -1.5 1,032,339 + LRK54_RS04860 0.73 -0.5 1,032,339 + LRK54_RS04860 0.73 +0.4 1,032,340 - LRK54_RS04860 0.73 -1.1 1,032,340 - LRK54_RS04860 0.73 -1.1 1,032,340 - LRK54_RS04860 0.73 +0.6 1,032,340 - LRK54_RS04860 0.73 +2.5 1,032,340 - LRK54_RS04860 0.73 +1.2 1,032,340 - LRK54_RS04860 0.73 +0.0 1,032,340 - LRK54_RS04860 0.73 +0.7 1,032,348 + LRK54_RS04860 0.74 -1.1 1,032,349 - LRK54_RS04860 0.74 -1.8 1,032,349 - LRK54_RS04860 0.74 +0.6 1,032,349 - LRK54_RS04860 0.74 -3.3 1,032,531 + +0.9 1,032,532 - +2.9 1,032,535 + -0.7 1,032,535 + -0.4 1,032,535 + -1.2 1,032,535 + +0.2 1,032,535 + -1.0 1,032,535 + -0.7 1,032,535 + +0.5 1,032,535 + +0.5 1,032,535 + -1.8 1,032,536 - -0.0 1,032,536 - +2.6 1,032,536 - -0.4 1,032,536 - +0.0 1,032,536 - +2.6 1,032,536 - -1.3 1,032,536 - +0.7 1,032,536 - +2.5 1,032,536 - -0.7 1,032,536 - -0.5 1,032,536 - +1.3 1,032,536 - -2.1 1,032,608 + -0.4 1,032,608 + -1.1 1,032,608 + -0.6 1,032,608 + -2.0 1,032,608 + +0.4 1,032,608 + +1.0 1,032,609 - -0.6 1,032,609 - -0.1 1,032,609 - -1.6 1,032,609 - +1.4 1,032,609 - +0.0 1,032,675 - LRK54_RS04865 0.17 -1.6 1,032,761 + LRK54_RS04865 0.27 -1.9 1,032,761 + LRK54_RS04865 0.27 -0.1 1,032,762 - LRK54_RS04865 0.27 -0.9 1,032,762 - LRK54_RS04865 0.27 -1.4 1,032,776 + LRK54_RS04865 0.29 -0.9 1,032,776 + LRK54_RS04865 0.29 -1.9 1,032,776 + LRK54_RS04865 0.29 -0.9 1,032,776 + LRK54_RS04865 0.29 +1.1 1,032,776 + LRK54_RS04865 0.29 -3.4 1,032,776 + LRK54_RS04865 0.29 -0.2 1,032,776 + LRK54_RS04865 0.29 +0.4 1,032,776 + LRK54_RS04865 0.29 -1.7 1,032,776 + LRK54_RS04865 0.29 +0.9 1,032,777 - LRK54_RS04865 0.29 +2.4 1,032,777 - LRK54_RS04865 0.29 -0.7 1,032,777 - LRK54_RS04865 0.29 +0.7 1,032,777 - LRK54_RS04865 0.29 -2.5 1,032,777 - LRK54_RS04865 0.29 +0.6 1,032,777 - LRK54_RS04865 0.29 -1.9 1,032,777 - LRK54_RS04865 0.29 -1.3 1,032,777 - LRK54_RS04865 0.29 -1.1 1,032,777 - LRK54_RS04865 0.29 +0.4 1,032,777 - LRK54_RS04865 0.29 -1.2 1,032,777 - LRK54_RS04865 0.29 -1.2 1,032,777 - LRK54_RS04865 0.29 -1.6 1,032,777 - LRK54_RS04865 0.29 -1.0 1,032,777 - LRK54_RS04865 0.29 -1.0 1,032,777 - LRK54_RS04865 0.29 -0.6 1,032,777 - LRK54_RS04865 0.29 -1.2 1,032,779 + LRK54_RS04865 0.29 +1.2 1,032,779 + LRK54_RS04865 0.29 -1.1 1,032,779 + LRK54_RS04865 0.29 -1.1 1,032,779 + LRK54_RS04865 0.29 +0.9 1,032,780 - LRK54_RS04865 0.29 +0.0 1,032,780 - LRK54_RS04865 0.29 +0.8 1,032,780 - LRK54_RS04865 0.29 -0.5 1,032,780 - LRK54_RS04865 0.29 -1.0 1,032,780 - LRK54_RS04865 0.29 -0.1 1,032,780 - LRK54_RS04865 0.29 +1.0 1,032,780 - LRK54_RS04865 0.29 -0.1 1,032,780 - LRK54_RS04865 0.29 -0.7 1,032,780 - LRK54_RS04865 0.29 -2.2 1,032,780 - LRK54_RS04865 0.29 +1.2 1,032,780 - LRK54_RS04865 0.29 -1.3 1,032,780 - LRK54_RS04865 0.29 -0.2 1,032,780 - LRK54_RS04865 0.29 -2.0 1,032,780 - LRK54_RS04865 0.29 -1.7 1,032,780 - LRK54_RS04865 0.29 -0.8 1,032,797 + LRK54_RS04865 0.32 -1.4 1,032,798 - LRK54_RS04865 0.32 -2.0 1,032,798 - LRK54_RS04865 0.32 -0.5 1,032,798 - LRK54_RS04865 0.32 -0.5 1,032,798 - LRK54_RS04865 0.32 -1.8 1,032,798 - LRK54_RS04865 0.32 +1.4 1,032,830 + LRK54_RS04865 0.36 +0.1 1,032,831 - LRK54_RS04865 0.36 -2.9 1,032,831 - LRK54_RS04865 0.36 -0.7 1,032,831 - LRK54_RS04865 0.36 -1.8 1,032,872 + LRK54_RS04865 0.41 -2.0 1,032,872 + LRK54_RS04865 0.41 +1.7 1,032,872 + LRK54_RS04865 0.41 -1.3 1,032,872 + LRK54_RS04865 0.41 +1.5 1,032,872 + LRK54_RS04865 0.41 -1.5 1,032,872 + LRK54_RS04865 0.41 -0.5 1,032,872 + LRK54_RS04865 0.41 +0.3 1,032,872 + LRK54_RS04865 0.41 -1.5 1,032,872 + LRK54_RS04865 0.41 -2.8 1,032,872 + LRK54_RS04865 0.41 -0.9 1,032,872 + LRK54_RS04865 0.41 -0.1 1,032,872 + LRK54_RS04865 0.41 -1.5 1,032,872 + LRK54_RS04865 0.41 -1.1 1,032,872 + LRK54_RS04865 0.41 -1.3 1,032,872 + LRK54_RS04865 0.41 -1.2 1,032,872 + LRK54_RS04865 0.41 -1.6 1,032,872 + LRK54_RS04865 0.41 +0.1 1,032,872 + LRK54_RS04865 0.41 +0.2 1,032,872 + LRK54_RS04865 0.41 +0.2 1,032,872 + LRK54_RS04865 0.41 +0.4 1,032,872 + LRK54_RS04865 0.41 -1.7 1,032,873 - LRK54_RS04865 0.41 +0.7 1,032,873 - LRK54_RS04865 0.41 -0.4 1,032,873 - LRK54_RS04865 0.41 -1.8 1,032,873 - LRK54_RS04865 0.41 -0.6 1,032,873 - LRK54_RS04865 0.41 -1.5 1,032,873 - LRK54_RS04865 0.41 -2.4 1,032,873 - LRK54_RS04865 0.41 -0.2 1,032,873 - LRK54_RS04865 0.41 -0.9 1,032,873 - LRK54_RS04865 0.41 +0.1 1,032,873 - LRK54_RS04865 0.41 -1.1 1,032,873 - LRK54_RS04865 0.41 +2.4 1,032,873 - LRK54_RS04865 0.41 -0.6 1,032,873 - LRK54_RS04865 0.41 -1.0 1,032,873 - LRK54_RS04865 0.41 +0.4 1,032,873 - LRK54_RS04865 0.41 -0.4 1,032,873 - LRK54_RS04865 0.41 -1.1 1,032,873 - LRK54_RS04865 0.41 -1.6 1,032,873 - LRK54_RS04865 0.41 +0.2 1,032,873 - LRK54_RS04865 0.41 -1.3 1,032,873 - LRK54_RS04865 0.41 +0.2 1,032,873 - LRK54_RS04865 0.41 -0.6 1,032,874 - LRK54_RS04865 0.41 +0.4 1,032,908 + LRK54_RS04865 0.45 -0.7 1,032,908 + LRK54_RS04865 0.45 -0.6 1,032,909 - LRK54_RS04865 0.45 -1.0 1,033,118 + LRK54_RS04865 0.71 -0.2 1,033,118 + LRK54_RS04865 0.71 -0.7 1,033,118 + LRK54_RS04865 0.71 -0.3 1,033,118 + LRK54_RS04865 0.71 -1.9 1,033,119 - LRK54_RS04865 0.71 -1.5 1,033,119 - LRK54_RS04865 0.71 -0.6 1,033,119 - LRK54_RS04865 0.71 -0.3 1,033,119 - LRK54_RS04865 0.71 +0.6 1,033,119 - LRK54_RS04865 0.71 -1.9 1,033,403 - +0.9 1,033,990 - +0.1 1,033,990 - -0.1 1,034,016 - +0.2 1,034,016 - +2.0 1,034,016 - +1.2 1,034,016 - -0.6 1,034,016 - -0.1 1,034,016 - -0.7 1,034,016 - -1.6 1,034,016 - +0.2 1,034,016 - -2.1 1,034,016 - -0.2 1,034,016 - -2.7 1,034,016 - -2.3 1,034,016 - +0.1 1,034,023 - -2.1 1,034,023 - -1.7 1,034,023 - +0.3 1,034,023 - -1.3
Or see this region's nucleotide sequence