Experiment: R2A with Nickel (II) chloride 0.3125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS03355 and LRK54_RS03360 are separated by 75 nucleotides LRK54_RS03360 and LRK54_RS03365 overlap by 17 nucleotides LRK54_RS03365 and LRK54_RS03370 are separated by 64 nucleotides LRK54_RS03370 and LRK54_RS03375 are separated by 238 nucleotides
LRK54_RS03355: LRK54_RS03355 - tRNA lysidine(34) synthetase TilS, at 722,600 to 723,904
_RS03355
LRK54_RS03360: LRK54_RS03360 - exodeoxyribonuclease VII small subunit, at 723,980 to 724,237
_RS03360
LRK54_RS03365: LRK54_RS03365 - polyprenyl synthetase family protein, at 724,221 to 725,120
_RS03365
LRK54_RS03370: LRK54_RS03370 - DUF4870 domain-containing protein, at 725,185 to 725,586
_RS03370
LRK54_RS03375: LRK54_RS03375 - metalloprotease PmbA, at 725,825 to 727,192
_RS03375
Position (kb) 724
725
726 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 724.311 kb on + strand, within LRK54_RS03365 at 724.311 kb on + strand, within LRK54_RS03365 at 724.311 kb on + strand, within LRK54_RS03365 at 724.312 kb on - strand, within LRK54_RS03365 at 724.312 kb on - strand, within LRK54_RS03365 at 724.312 kb on - strand, within LRK54_RS03365 at 724.312 kb on - strand, within LRK54_RS03365 at 724.312 kb on - strand, within LRK54_RS03365 at 724.344 kb on + strand, within LRK54_RS03365 at 724.345 kb on - strand, within LRK54_RS03365 at 724.345 kb on - strand, within LRK54_RS03365 at 724.389 kb on + strand, within LRK54_RS03365 at 724.389 kb on + strand, within LRK54_RS03365 at 724.390 kb on - strand, within LRK54_RS03365 at 724.390 kb on - strand, within LRK54_RS03365 at 724.390 kb on - strand, within LRK54_RS03365 at 724.390 kb on - strand, within LRK54_RS03365 at 724.390 kb on - strand, within LRK54_RS03365 at 724.467 kb on + strand, within LRK54_RS03365 at 724.467 kb on + strand, within LRK54_RS03365 at 724.467 kb on + strand, within LRK54_RS03365 at 724.467 kb on + strand, within LRK54_RS03365 at 724.467 kb on + strand, within LRK54_RS03365 at 724.467 kb on + strand, within LRK54_RS03365 at 724.468 kb on - strand, within LRK54_RS03365 at 724.468 kb on - strand, within LRK54_RS03365 at 724.468 kb on - strand, within LRK54_RS03365 at 725.098 kb on - strand at 725.098 kb on - strand at 725.115 kb on + strand at 725.115 kb on + strand at 725.116 kb on - strand at 725.116 kb on - strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.219 kb on + strand at 725.220 kb on - strand at 725.220 kb on - strand at 725.220 kb on - strand at 725.220 kb on - strand at 725.220 kb on - strand at 725.220 kb on - strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.600 kb on + strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.601 kb on - strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.653 kb on + strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.654 kb on - strand at 725.811 kb on + strand at 725.811 kb on + strand at 725.811 kb on + strand at 725.811 kb on + strand at 725.811 kb on + strand at 725.812 kb on - strand at 725.812 kb on - strand at 725.812 kb on - strand at 725.812 kb on - strand at 725.823 kb on + strand at 725.823 kb on + strand at 725.823 kb on + strand at 725.823 kb on + strand at 725.823 kb on + strand at 725.823 kb on + strand at 725.823 kb on + strand at 725.823 kb on + strand at 725.824 kb on - strand at 725.824 kb on - strand at 725.824 kb on - strand at 725.824 kb on - strand at 725.824 kb on - strand at 725.824 kb on - strand at 725.824 kb on - strand at 725.824 kb on - strand at 725.826 kb on + strand at 725.826 kb on + strand at 725.826 kb on + strand at 725.826 kb on + strand at 725.826 kb on + strand at 725.826 kb on + strand at 725.826 kb on + strand at 725.826 kb on + strand at 725.826 kb on + strand at 725.827 kb on - strand at 725.827 kb on - strand at 725.827 kb on - strand at 725.827 kb on - strand at 725.827 kb on - strand at 725.827 kb on - strand at 725.827 kb on - strand at 725.827 kb on - strand at 725.827 kb on - strand at 725.827 kb on - strand at 725.967 kb on + strand, within LRK54_RS03375 at 726.015 kb on + strand, within LRK54_RS03375 at 726.015 kb on + strand, within LRK54_RS03375 at 726.016 kb on - strand, within LRK54_RS03375 at 726.016 kb on - strand, within LRK54_RS03375 at 726.016 kb on - strand, within LRK54_RS03375
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.3125 mM remove 724,311 + LRK54_RS03365 0.10 -0.7 724,311 + LRK54_RS03365 0.10 -2.0 724,311 + LRK54_RS03365 0.10 +1.7 724,312 - LRK54_RS03365 0.10 -0.9 724,312 - LRK54_RS03365 0.10 +0.1 724,312 - LRK54_RS03365 0.10 -1.3 724,312 - LRK54_RS03365 0.10 +0.1 724,312 - LRK54_RS03365 0.10 -0.3 724,344 + LRK54_RS03365 0.14 -0.7 724,345 - LRK54_RS03365 0.14 -0.8 724,345 - LRK54_RS03365 0.14 -0.7 724,389 + LRK54_RS03365 0.19 -0.4 724,389 + LRK54_RS03365 0.19 -1.6 724,390 - LRK54_RS03365 0.19 -0.1 724,390 - LRK54_RS03365 0.19 -1.4 724,390 - LRK54_RS03365 0.19 -1.9 724,390 - LRK54_RS03365 0.19 +0.1 724,390 - LRK54_RS03365 0.19 -0.8 724,467 + LRK54_RS03365 0.27 -1.2 724,467 + LRK54_RS03365 0.27 -0.2 724,467 + LRK54_RS03365 0.27 -2.5 724,467 + LRK54_RS03365 0.27 +3.1 724,467 + LRK54_RS03365 0.27 -1.8 724,467 + LRK54_RS03365 0.27 -0.6 724,468 - LRK54_RS03365 0.27 +0.2 724,468 - LRK54_RS03365 0.27 -0.1 724,468 - LRK54_RS03365 0.27 +0.4 725,098 - -0.3 725,098 - +0.5 725,115 + -3.5 725,115 + -0.8 725,116 - +0.1 725,116 - -0.1 725,219 + +2.1 725,219 + -0.7 725,219 + -1.3 725,219 + -0.2 725,219 + -0.5 725,219 + +1.0 725,219 + +0.2 725,219 + -1.0 725,219 + -1.6 725,219 + -1.2 725,219 + +0.3 725,219 + -0.8 725,220 - -0.7 725,220 - +0.6 725,220 - -0.9 725,220 - -3.6 725,220 - -2.2 725,220 - +0.1 725,600 + +0.4 725,600 + +0.2 725,600 + -1.2 725,600 + -2.2 725,600 + -0.2 725,600 + -0.9 725,600 + +1.2 725,600 + +0.1 725,600 + +0.2 725,600 + +0.2 725,600 + +0.7 725,600 + -0.2 725,601 - -0.4 725,601 - -0.4 725,601 - -0.5 725,601 - -1.5 725,601 - +0.3 725,601 - -0.7 725,601 - +0.4 725,601 - +1.7 725,601 - -0.6 725,601 - +0.3 725,601 - +1.6 725,601 - -0.2 725,653 + +0.8 725,653 + -0.4 725,653 + -0.8 725,653 + +0.6 725,653 + -0.2 725,653 + +0.2 725,653 + +0.1 725,653 + +0.8 725,653 + -0.7 725,653 + +0.8 725,653 + -1.2 725,653 + -0.9 725,653 + -2.1 725,653 + +0.3 725,653 + +0.2 725,653 + -0.2 725,653 + -0.2 725,653 + -0.3 725,654 - -1.1 725,654 - +0.8 725,654 - +0.5 725,654 - -1.2 725,654 - -1.3 725,654 - -0.5 725,654 - -0.9 725,654 - +1.2 725,654 - +2.1 725,654 - +1.6 725,654 - -0.9 725,654 - -1.2 725,654 - -0.5 725,811 + +0.8 725,811 + +1.4 725,811 + +2.9 725,811 + +0.7 725,811 + +0.4 725,812 - -0.6 725,812 - +0.8 725,812 - -0.3 725,812 - -0.1 725,823 + -1.2 725,823 + +1.3 725,823 + -0.4 725,823 + -1.4 725,823 + -2.7 725,823 + +1.8 725,823 + -0.5 725,823 + +0.1 725,824 - -0.0 725,824 - -0.7 725,824 - +0.8 725,824 - -1.4 725,824 - +0.8 725,824 - +0.7 725,824 - -1.3 725,824 - -0.1 725,826 + -1.1 725,826 + -0.7 725,826 + +0.7 725,826 + +0.3 725,826 + +0.5 725,826 + -0.2 725,826 + -0.6 725,826 + +0.6 725,826 + +0.2 725,827 - +1.0 725,827 - -0.7 725,827 - +0.0 725,827 - +1.7 725,827 - -0.2 725,827 - +0.3 725,827 - -2.1 725,827 - -0.7 725,827 - +5.0 725,827 - -2.2 725,967 + LRK54_RS03375 0.10 -2.9 726,015 + LRK54_RS03375 0.14 -1.8 726,015 + LRK54_RS03375 0.14 -2.2 726,016 - LRK54_RS03375 0.14 -0.2 726,016 - LRK54_RS03375 0.14 -1.2 726,016 - LRK54_RS03375 0.14 +2.3
Or see this region's nucleotide sequence