Experiment: R2A with Zinc 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS17420 and CA265_RS17425 are separated by 103 nucleotides CA265_RS17425 and CA265_RS17430 are separated by 53 nucleotides CA265_RS17430 and CA265_RS17435 are separated by 93 nucleotides CA265_RS17435 and CA265_RS17440 are separated by 323 nucleotides
CA265_RS17420: CA265_RS17420 - glyoxalase, at 4,347,224 to 4,347,643
_RS17420
CA265_RS17425: CA265_RS17425 - tRNA-Val, at 4,347,747 to 4,347,821
_RS17425
CA265_RS17430: CA265_RS17430 - tRNA-Val, at 4,347,875 to 4,347,952
_RS17430
CA265_RS17435: CA265_RS17435 - DNA repair protein RecO, at 4,348,046 to 4,348,771
_RS17435
CA265_RS17440: CA265_RS17440 - Fis family transcriptional regulator, at 4,349,095 to 4,351,245
_RS17440
Position (kb)
4348
4349 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 4347.047 kb on - strand at 4347.047 kb on - strand at 4347.088 kb on - strand at 4347.155 kb on - strand at 4347.171 kb on - strand at 4347.246 kb on + strand at 4347.247 kb on - strand at 4347.374 kb on - strand, within CA265_RS17420 at 4347.491 kb on - strand, within CA265_RS17420 at 4347.553 kb on - strand, within CA265_RS17420 at 4347.553 kb on - strand, within CA265_RS17420 at 4347.553 kb on - strand, within CA265_RS17420 at 4347.573 kb on - strand, within CA265_RS17420 at 4347.595 kb on + strand, within CA265_RS17420 at 4347.596 kb on - strand, within CA265_RS17420 at 4347.596 kb on - strand, within CA265_RS17420 at 4347.596 kb on - strand, within CA265_RS17420 at 4347.684 kb on + strand at 4347.684 kb on + strand at 4347.684 kb on + strand at 4347.685 kb on - strand at 4347.685 kb on - strand at 4347.728 kb on + strand at 4347.744 kb on - strand at 4347.829 kb on + strand at 4348.082 kb on + strand at 4348.106 kb on + strand at 4348.125 kb on - strand, within CA265_RS17435 at 4348.129 kb on + strand, within CA265_RS17435 at 4348.167 kb on - strand, within CA265_RS17435 at 4348.192 kb on + strand, within CA265_RS17435 at 4348.207 kb on + strand, within CA265_RS17435 at 4348.249 kb on + strand, within CA265_RS17435 at 4348.472 kb on + strand, within CA265_RS17435 at 4348.496 kb on - strand, within CA265_RS17435 at 4348.496 kb on - strand, within CA265_RS17435 at 4348.588 kb on - strand, within CA265_RS17435 at 4348.671 kb on + strand, within CA265_RS17435 at 4348.671 kb on + strand, within CA265_RS17435 at 4348.729 kb on - strand at 4348.729 kb on - strand at 4348.797 kb on + strand at 4348.797 kb on + strand at 4348.868 kb on + strand at 4348.871 kb on + strand at 4348.871 kb on + strand at 4348.959 kb on - strand at 4348.978 kb on + strand at 4349.053 kb on + strand at 4349.102 kb on + strand at 4349.108 kb on + strand at 4349.108 kb on + strand at 4349.187 kb on + strand at 4349.202 kb on - strand at 4349.284 kb on - strand at 4349.324 kb on - strand, within CA265_RS17440 at 4349.325 kb on + strand, within CA265_RS17440 at 4349.325 kb on + strand, within CA265_RS17440 at 4349.421 kb on + strand, within CA265_RS17440 at 4349.421 kb on + strand, within CA265_RS17440 at 4349.421 kb on + strand, within CA265_RS17440 at 4349.421 kb on + strand, within CA265_RS17440 at 4349.427 kb on + strand, within CA265_RS17440 at 4349.428 kb on - strand, within CA265_RS17440 at 4349.490 kb on + strand, within CA265_RS17440 at 4349.490 kb on + strand, within CA265_RS17440 at 4349.491 kb on - strand, within CA265_RS17440 at 4349.527 kb on - strand, within CA265_RS17440 at 4349.636 kb on + strand, within CA265_RS17440 at 4349.655 kb on - strand, within CA265_RS17440 at 4349.655 kb on - strand, within CA265_RS17440 at 4349.727 kb on + strand, within CA265_RS17440 at 4349.736 kb on + strand, within CA265_RS17440 at 4349.770 kb on - strand, within CA265_RS17440
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Zinc 0.2 mM remove 4,347,047 - +0.4 4,347,047 - -0.4 4,347,088 - -1.5 4,347,155 - +1.8 4,347,171 - +1.0 4,347,246 + -1.8 4,347,247 - -0.9 4,347,374 - CA265_RS17420 0.36 -1.5 4,347,491 - CA265_RS17420 0.64 +0.8 4,347,553 - CA265_RS17420 0.78 +0.3 4,347,553 - CA265_RS17420 0.78 +0.6 4,347,553 - CA265_RS17420 0.78 -0.0 4,347,573 - CA265_RS17420 0.83 -0.3 4,347,595 + CA265_RS17420 0.88 -0.2 4,347,596 - CA265_RS17420 0.89 +1.7 4,347,596 - CA265_RS17420 0.89 -3.3 4,347,596 - CA265_RS17420 0.89 +0.9 4,347,684 + +1.0 4,347,684 + +0.7 4,347,684 + +1.2 4,347,685 - -0.3 4,347,685 - -0.8 4,347,728 + +0.1 4,347,744 - -0.1 4,347,829 + +1.7 4,348,082 + -2.8 4,348,106 + -0.2 4,348,125 - CA265_RS17435 0.11 -2.1 4,348,129 + CA265_RS17435 0.11 +0.4 4,348,167 - CA265_RS17435 0.17 +0.7 4,348,192 + CA265_RS17435 0.20 -0.2 4,348,207 + CA265_RS17435 0.22 -0.4 4,348,249 + CA265_RS17435 0.28 -1.1 4,348,472 + CA265_RS17435 0.59 +1.1 4,348,496 - CA265_RS17435 0.62 -1.8 4,348,496 - CA265_RS17435 0.62 -0.3 4,348,588 - CA265_RS17435 0.75 -0.4 4,348,671 + CA265_RS17435 0.86 -1.3 4,348,671 + CA265_RS17435 0.86 -1.2 4,348,729 - +1.4 4,348,729 - -0.9 4,348,797 + -1.2 4,348,797 + -0.1 4,348,868 + -0.6 4,348,871 + -1.2 4,348,871 + +0.4 4,348,959 - +2.3 4,348,978 + +0.9 4,349,053 + +0.4 4,349,102 + +1.8 4,349,108 + +0.9 4,349,108 + -1.1 4,349,187 + +0.9 4,349,202 - -0.3 4,349,284 - +2.9 4,349,324 - CA265_RS17440 0.11 -0.6 4,349,325 + CA265_RS17440 0.11 +0.9 4,349,325 + CA265_RS17440 0.11 +1.9 4,349,421 + CA265_RS17440 0.15 +0.5 4,349,421 + CA265_RS17440 0.15 +1.8 4,349,421 + CA265_RS17440 0.15 +1.3 4,349,421 + CA265_RS17440 0.15 +2.9 4,349,427 + CA265_RS17440 0.15 +1.1 4,349,428 - CA265_RS17440 0.15 +2.5 4,349,490 + CA265_RS17440 0.18 +5.3 4,349,490 + CA265_RS17440 0.18 +2.6 4,349,491 - CA265_RS17440 0.18 +2.1 4,349,527 - CA265_RS17440 0.20 +1.6 4,349,636 + CA265_RS17440 0.25 +0.1 4,349,655 - CA265_RS17440 0.26 -1.6 4,349,655 - CA265_RS17440 0.26 +2.2 4,349,727 + CA265_RS17440 0.29 -1.4 4,349,736 + CA265_RS17440 0.30 +1.5 4,349,770 - CA265_RS17440 0.31 -1.2
Or see this region's nucleotide sequence