Experiment: Beta-Lactose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02029 and CCNA_02030 are separated by 2 nucleotides CCNA_02030 and CCNA_02031 are separated by 21 nucleotides CCNA_02031 and CCNA_02032 overlap by 4 nucleotides CCNA_02032 and CCNA_02033 overlap by 31 nucleotides
CCNA_02029: CCNA_02029 - NADH-quinone oxidoreductase chain D, at 2,174,263 to 2,175,513
_02029
CCNA_02030: CCNA_02030 - hypothetical protein, at 2,175,516 to 2,175,911
_02030
CCNA_02031: CCNA_02031 - NADH-quinone oxidoreductase chain C, at 2,175,933 to 2,176,526
_02031
CCNA_02032: CCNA_02032 - NADH-quinone oxidoreductase chain B, at 2,176,523 to 2,177,125
_02032
CCNA_02033: CCNA_02033 - NADH-quinone oxidoreductase chain A, at 2,177,095 to 2,177,472
_02033
Position (kb)
2175
2176
2177 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 2175.678 kb on - strand, within CCNA_02030 at 2175.782 kb on - strand, within CCNA_02030 at 2175.810 kb on - strand, within CCNA_02030 at 2175.914 kb on - strand at 2175.941 kb on - strand at 2177.475 kb on - strand at 2177.498 kb on + strand at 2177.498 kb on + strand at 2177.506 kb on - strand at 2177.506 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose (C) remove 2,175,678 - CCNA_02030 0.41 +3.8 2,175,782 - CCNA_02030 0.67 +3.3 2,175,810 - CCNA_02030 0.74 +3.3 2,175,914 - +3.9 2,175,941 - +3.5 2,177,475 - +4.9 2,177,498 + +0.7 2,177,498 + +1.8 2,177,506 - +4.3 2,177,506 - +2.6
Or see this region's nucleotide sequence