Experiment: R2A with Cisplatin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS06540 and CA265_RS06545 are separated by 418 nucleotides CA265_RS06545 and CA265_RS06550 are separated by 2 nucleotides
CA265_RS06540: CA265_RS06540 - flagellar motor protein MotB, at 1,614,230 to 1,616,191
_RS06540
CA265_RS06545: CA265_RS06545 - DNA polymerase IV, at 1,616,610 to 1,617,929
_RS06545
CA265_RS06550: CA265_RS06550 - DNA polymerase III subunit alpha, at 1,617,932 to 1,620,946
_RS06550
Position (kb)
1616
1617
1618 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1615.715 kb on + strand, within CA265_RS06540 at 1615.716 kb on - strand, within CA265_RS06540 at 1615.716 kb on - strand, within CA265_RS06540 at 1615.794 kb on + strand, within CA265_RS06540 at 1615.843 kb on + strand, within CA265_RS06540 at 1615.860 kb on - strand, within CA265_RS06540 at 1615.903 kb on - strand, within CA265_RS06540 at 1615.912 kb on + strand, within CA265_RS06540 at 1615.937 kb on - strand, within CA265_RS06540 at 1615.994 kb on + strand, within CA265_RS06540 at 1615.995 kb on - strand at 1615.995 kb on - strand at 1616.002 kb on - strand at 1616.034 kb on + strand at 1616.039 kb on + strand at 1616.063 kb on - strand at 1616.088 kb on - strand at 1616.303 kb on - strand at 1616.303 kb on - strand at 1616.303 kb on - strand at 1616.348 kb on - strand at 1616.349 kb on + strand at 1616.380 kb on - strand at 1616.403 kb on - strand at 1616.412 kb on - strand at 1616.492 kb on - strand at 1616.549 kb on - strand at 1616.631 kb on - strand at 1616.684 kb on + strand at 1616.684 kb on + strand at 1616.752 kb on - strand, within CA265_RS06545 at 1617.003 kb on - strand, within CA265_RS06545 at 1617.174 kb on - strand, within CA265_RS06545 at 1617.180 kb on - strand, within CA265_RS06545 at 1617.377 kb on + strand, within CA265_RS06545 at 1617.469 kb on + strand, within CA265_RS06545 at 1617.470 kb on - strand, within CA265_RS06545 at 1617.470 kb on - strand, within CA265_RS06545 at 1617.476 kb on - strand, within CA265_RS06545 at 1617.481 kb on - strand, within CA265_RS06545 at 1617.482 kb on + strand, within CA265_RS06545 at 1617.523 kb on - strand, within CA265_RS06545 at 1617.551 kb on + strand, within CA265_RS06545 at 1617.577 kb on + strand, within CA265_RS06545 at 1617.578 kb on - strand, within CA265_RS06545 at 1617.657 kb on + strand, within CA265_RS06545 at 1617.658 kb on - strand, within CA265_RS06545 at 1617.659 kb on + strand, within CA265_RS06545 at 1617.678 kb on + strand, within CA265_RS06545 at 1617.680 kb on + strand, within CA265_RS06545 at 1617.680 kb on + strand, within CA265_RS06545 at 1617.707 kb on - strand, within CA265_RS06545 at 1617.746 kb on + strand, within CA265_RS06545 at 1617.921 kb on + strand at 1617.940 kb on - strand at 1617.956 kb on + strand at 1617.973 kb on + strand at 1618.032 kb on + strand at 1618.048 kb on + strand at 1618.065 kb on + strand at 1618.088 kb on - strand at 1618.123 kb on + strand at 1618.183 kb on + strand at 1618.184 kb on - strand at 1618.193 kb on + strand at 1618.193 kb on + strand at 1618.193 kb on + strand at 1618.194 kb on - strand at 1618.307 kb on + strand, within CA265_RS06550 at 1618.308 kb on - strand, within CA265_RS06550 at 1618.309 kb on + strand, within CA265_RS06550 at 1618.344 kb on + strand, within CA265_RS06550 at 1618.344 kb on + strand, within CA265_RS06550 at 1618.347 kb on + strand, within CA265_RS06550 at 1618.348 kb on - strand, within CA265_RS06550 at 1618.355 kb on - strand, within CA265_RS06550 at 1618.455 kb on + strand, within CA265_RS06550 at 1618.462 kb on + strand, within CA265_RS06550 at 1618.488 kb on - strand, within CA265_RS06550 at 1618.518 kb on + strand, within CA265_RS06550 at 1618.519 kb on - strand, within CA265_RS06550 at 1618.522 kb on + strand, within CA265_RS06550 at 1618.523 kb on - strand, within CA265_RS06550 at 1618.523 kb on - strand, within CA265_RS06550 at 1618.703 kb on + strand, within CA265_RS06550 at 1618.726 kb on + strand, within CA265_RS06550 at 1618.786 kb on + strand, within CA265_RS06550 at 1618.834 kb on + strand, within CA265_RS06550 at 1618.865 kb on + strand, within CA265_RS06550 at 1618.866 kb on - strand, within CA265_RS06550 at 1618.866 kb on - strand, within CA265_RS06550 at 1618.870 kb on + strand, within CA265_RS06550 at 1618.871 kb on - strand, within CA265_RS06550 at 1618.871 kb on - strand, within CA265_RS06550 at 1618.875 kb on + strand, within CA265_RS06550 at 1618.880 kb on + strand, within CA265_RS06550 at 1618.904 kb on + strand, within CA265_RS06550
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.005 mg/ml remove 1,615,715 + CA265_RS06540 0.76 -1.7 1,615,716 - CA265_RS06540 0.76 +0.1 1,615,716 - CA265_RS06540 0.76 +0.0 1,615,794 + CA265_RS06540 0.80 -0.3 1,615,843 + CA265_RS06540 0.82 +0.4 1,615,860 - CA265_RS06540 0.83 -0.6 1,615,903 - CA265_RS06540 0.85 -0.8 1,615,912 + CA265_RS06540 0.86 +0.4 1,615,937 - CA265_RS06540 0.87 -1.1 1,615,994 + CA265_RS06540 0.90 +0.4 1,615,995 - -0.3 1,615,995 - -0.1 1,616,002 - -1.3 1,616,034 + +0.1 1,616,039 + +0.6 1,616,063 - -0.5 1,616,088 - -0.2 1,616,303 - -1.2 1,616,303 - -0.0 1,616,303 - -0.8 1,616,348 - -0.5 1,616,349 + +0.2 1,616,380 - -1.1 1,616,403 - -0.3 1,616,412 - +0.1 1,616,492 - +0.3 1,616,549 - -0.3 1,616,631 - -1.7 1,616,684 + -1.0 1,616,684 + -1.6 1,616,752 - CA265_RS06545 0.11 -0.2 1,617,003 - CA265_RS06545 0.30 -1.6 1,617,174 - CA265_RS06545 0.43 -1.4 1,617,180 - CA265_RS06545 0.43 -1.6 1,617,377 + CA265_RS06545 0.58 -0.8 1,617,469 + CA265_RS06545 0.65 -0.6 1,617,470 - CA265_RS06545 0.65 -0.3 1,617,470 - CA265_RS06545 0.65 -1.7 1,617,476 - CA265_RS06545 0.66 -2.3 1,617,481 - CA265_RS06545 0.66 -0.6 1,617,482 + CA265_RS06545 0.66 -1.4 1,617,523 - CA265_RS06545 0.69 -1.1 1,617,551 + CA265_RS06545 0.71 -0.8 1,617,577 + CA265_RS06545 0.73 -0.6 1,617,578 - CA265_RS06545 0.73 -1.4 1,617,657 + CA265_RS06545 0.79 -0.5 1,617,658 - CA265_RS06545 0.79 -0.6 1,617,659 + CA265_RS06545 0.79 -0.8 1,617,678 + CA265_RS06545 0.81 -1.2 1,617,680 + CA265_RS06545 0.81 -0.1 1,617,680 + CA265_RS06545 0.81 +0.1 1,617,707 - CA265_RS06545 0.83 -1.5 1,617,746 + CA265_RS06545 0.86 -1.7 1,617,921 + -0.0 1,617,940 - -0.8 1,617,956 + -1.0 1,617,973 + -0.5 1,618,032 + -0.3 1,618,048 + -1.5 1,618,065 + -1.4 1,618,088 - -0.3 1,618,123 + -1.0 1,618,183 + +0.5 1,618,184 - -0.5 1,618,193 + +2.6 1,618,193 + -1.1 1,618,193 + -0.5 1,618,194 - +0.5 1,618,307 + CA265_RS06550 0.12 -0.2 1,618,308 - CA265_RS06550 0.12 -0.8 1,618,309 + CA265_RS06550 0.13 -1.7 1,618,344 + CA265_RS06550 0.14 -0.4 1,618,344 + CA265_RS06550 0.14 -0.1 1,618,347 + CA265_RS06550 0.14 +0.1 1,618,348 - CA265_RS06550 0.14 -1.2 1,618,355 - CA265_RS06550 0.14 -1.3 1,618,455 + CA265_RS06550 0.17 -0.3 1,618,462 + CA265_RS06550 0.18 -2.2 1,618,488 - CA265_RS06550 0.18 -0.6 1,618,518 + CA265_RS06550 0.19 -0.6 1,618,519 - CA265_RS06550 0.19 -1.1 1,618,522 + CA265_RS06550 0.20 -0.5 1,618,523 - CA265_RS06550 0.20 -0.5 1,618,523 - CA265_RS06550 0.20 -0.6 1,618,703 + CA265_RS06550 0.26 -0.9 1,618,726 + CA265_RS06550 0.26 -0.5 1,618,786 + CA265_RS06550 0.28 -1.1 1,618,834 + CA265_RS06550 0.30 -1.9 1,618,865 + CA265_RS06550 0.31 -1.4 1,618,866 - CA265_RS06550 0.31 +0.1 1,618,866 - CA265_RS06550 0.31 -0.9 1,618,870 + CA265_RS06550 0.31 -0.3 1,618,871 - CA265_RS06550 0.31 +1.1 1,618,871 - CA265_RS06550 0.31 -0.9 1,618,875 + CA265_RS06550 0.31 +0.7 1,618,880 + CA265_RS06550 0.31 -0.9 1,618,904 + CA265_RS06550 0.32 -1.7
Or see this region's nucleotide sequence