Experiment: Nickel (II) chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_4986 and Ac3H11_4987 are separated by 13 nucleotides Ac3H11_4987 and Ac3H11_4988 overlap by 4 nucleotides Ac3H11_4988 and Ac3H11_4989 are separated by 101 nucleotides
Ac3H11_4986 - High-affinity leucine-specific transport system, periplasmic binding protein LivK (TC 3.A.1.4.1), at 149,922 to 151,103
_4986
Ac3H11_4987 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 151,117 to 151,935
_4987
Ac3H11_4988 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 151,932 to 153,734
_4988
Ac3H11_4989 - Enoyl-CoA hydratase (EC 4.2.1.17), at 153,836 to 154,636
_4989
Position (kb)
151
152
153
154 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 151.171 kb on + strand at 151.171 kb on + strand at 151.171 kb on + strand at 151.171 kb on + strand at 151.171 kb on + strand at 151.172 kb on - strand at 151.172 kb on - strand at 151.172 kb on - strand at 151.462 kb on + strand, within Ac3H11_4987 at 151.462 kb on + strand, within Ac3H11_4987 at 151.462 kb on + strand, within Ac3H11_4987 at 151.462 kb on + strand, within Ac3H11_4987 at 151.462 kb on + strand, within Ac3H11_4987 at 151.462 kb on + strand, within Ac3H11_4987 at 151.462 kb on + strand, within Ac3H11_4987 at 151.463 kb on - strand, within Ac3H11_4987 at 151.463 kb on - strand, within Ac3H11_4987 at 151.463 kb on - strand, within Ac3H11_4987 at 151.463 kb on - strand, within Ac3H11_4987 at 151.463 kb on - strand, within Ac3H11_4987 at 151.463 kb on - strand, within Ac3H11_4987 at 151.463 kb on - strand, within Ac3H11_4987 at 151.485 kb on + strand, within Ac3H11_4987 at 151.485 kb on + strand, within Ac3H11_4987 at 151.485 kb on + strand, within Ac3H11_4987 at 151.486 kb on - strand, within Ac3H11_4987 at 151.486 kb on - strand, within Ac3H11_4987 at 151.510 kb on + strand, within Ac3H11_4987 at 151.510 kb on + strand, within Ac3H11_4987 at 151.510 kb on + strand, within Ac3H11_4987 at 151.511 kb on - strand, within Ac3H11_4987 at 151.511 kb on - strand, within Ac3H11_4987 at 151.516 kb on + strand, within Ac3H11_4987 at 151.516 kb on + strand, within Ac3H11_4987 at 151.516 kb on + strand, within Ac3H11_4987 at 151.516 kb on + strand, within Ac3H11_4987 at 151.516 kb on + strand, within Ac3H11_4987 at 151.516 kb on + strand, within Ac3H11_4987 at 151.516 kb on + strand, within Ac3H11_4987 at 151.517 kb on - strand, within Ac3H11_4987 at 151.559 kb on - strand, within Ac3H11_4987 at 151.676 kb on + strand, within Ac3H11_4987 at 151.676 kb on + strand, within Ac3H11_4987 at 151.676 kb on + strand, within Ac3H11_4987 at 151.676 kb on + strand, within Ac3H11_4987 at 151.676 kb on + strand, within Ac3H11_4987 at 151.676 kb on + strand, within Ac3H11_4987 at 151.676 kb on + strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.774 kb on + strand, within Ac3H11_4987 at 151.775 kb on - strand, within Ac3H11_4987 at 151.852 kb on + strand, within Ac3H11_4987 at 151.906 kb on + strand at 151.906 kb on + strand at 151.907 kb on - strand at 151.907 kb on - strand at 151.907 kb on - strand at 151.907 kb on - strand at 151.907 kb on - strand at 152.055 kb on + strand at 152.206 kb on - strand, within Ac3H11_4988 at 152.206 kb on - strand, within Ac3H11_4988 at 152.235 kb on + strand, within Ac3H11_4988 at 152.235 kb on + strand, within Ac3H11_4988 at 152.235 kb on + strand, within Ac3H11_4988 at 152.235 kb on + strand, within Ac3H11_4988 at 152.235 kb on + strand, within Ac3H11_4988 at 152.236 kb on - strand, within Ac3H11_4988 at 152.236 kb on - strand, within Ac3H11_4988 at 152.236 kb on - strand, within Ac3H11_4988 at 152.502 kb on + strand, within Ac3H11_4988 at 152.502 kb on + strand, within Ac3H11_4988 at 152.502 kb on + strand, within Ac3H11_4988 at 152.503 kb on - strand, within Ac3H11_4988 at 152.503 kb on - strand, within Ac3H11_4988 at 152.503 kb on - strand, within Ac3H11_4988 at 152.623 kb on - strand, within Ac3H11_4988 at 152.623 kb on - strand, within Ac3H11_4988 at 152.623 kb on - strand, within Ac3H11_4988 at 152.854 kb on - strand, within Ac3H11_4988 at 152.919 kb on + strand, within Ac3H11_4988 at 152.919 kb on + strand, within Ac3H11_4988 at 152.920 kb on - strand, within Ac3H11_4988 at 152.931 kb on + strand, within Ac3H11_4988 at 152.931 kb on + strand, within Ac3H11_4988 at 152.931 kb on + strand, within Ac3H11_4988 at 152.932 kb on - strand, within Ac3H11_4988 at 152.932 kb on - strand, within Ac3H11_4988 at 153.066 kb on + strand, within Ac3H11_4988 at 153.066 kb on + strand, within Ac3H11_4988 at 153.066 kb on + strand, within Ac3H11_4988 at 153.066 kb on + strand, within Ac3H11_4988 at 153.066 kb on + strand, within Ac3H11_4988 at 153.067 kb on - strand, within Ac3H11_4988 at 153.067 kb on - strand, within Ac3H11_4988 at 153.067 kb on - strand, within Ac3H11_4988 at 153.067 kb on - strand, within Ac3H11_4988 at 153.067 kb on - strand, within Ac3H11_4988 at 153.067 kb on - strand, within Ac3H11_4988 at 153.219 kb on + strand, within Ac3H11_4988 at 153.220 kb on - strand, within Ac3H11_4988 at 153.220 kb on - strand, within Ac3H11_4988 at 153.220 kb on - strand, within Ac3H11_4988 at 153.220 kb on - strand, within Ac3H11_4988 at 153.516 kb on + strand, within Ac3H11_4988 at 153.693 kb on + strand at 153.693 kb on + strand at 153.693 kb on + strand at 153.694 kb on - strand at 153.694 kb on - strand at 153.702 kb on + strand at 153.702 kb on + strand at 153.702 kb on + strand at 153.703 kb on - strand at 153.703 kb on - strand at 153.726 kb on + strand at 153.726 kb on + strand at 153.727 kb on - strand at 153.796 kb on + strand at 153.797 kb on - strand at 154.214 kb on + strand, within Ac3H11_4989 at 154.214 kb on + strand, within Ac3H11_4989 at 154.214 kb on + strand, within Ac3H11_4989 at 154.215 kb on - strand, within Ac3H11_4989 at 154.253 kb on + strand, within Ac3H11_4989 at 154.253 kb on + strand, within Ac3H11_4989 at 154.254 kb on - strand, within Ac3H11_4989 at 154.254 kb on - strand, within Ac3H11_4989 at 154.472 kb on + strand, within Ac3H11_4989 at 154.726 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.6 mM remove 151,171 + +0.8 151,171 + -0.8 151,171 + +0.7 151,171 + -2.5 151,171 + -1.8 151,172 - -0.0 151,172 - -2.5 151,172 - -0.3 151,462 + Ac3H11_4987 0.42 -0.8 151,462 + Ac3H11_4987 0.42 -0.5 151,462 + Ac3H11_4987 0.42 -1.8 151,462 + Ac3H11_4987 0.42 -0.8 151,462 + Ac3H11_4987 0.42 -0.7 151,462 + Ac3H11_4987 0.42 +1.1 151,462 + Ac3H11_4987 0.42 -0.7 151,463 - Ac3H11_4987 0.42 -0.1 151,463 - Ac3H11_4987 0.42 +0.6 151,463 - Ac3H11_4987 0.42 -0.1 151,463 - Ac3H11_4987 0.42 +0.2 151,463 - Ac3H11_4987 0.42 +1.0 151,463 - Ac3H11_4987 0.42 +0.9 151,463 - Ac3H11_4987 0.42 +1.7 151,485 + Ac3H11_4987 0.45 -0.2 151,485 + Ac3H11_4987 0.45 -0.4 151,485 + Ac3H11_4987 0.45 +0.9 151,486 - Ac3H11_4987 0.45 +1.1 151,486 - Ac3H11_4987 0.45 +0.1 151,510 + Ac3H11_4987 0.48 +1.2 151,510 + Ac3H11_4987 0.48 -0.1 151,510 + Ac3H11_4987 0.48 -3.6 151,511 - Ac3H11_4987 0.48 -0.1 151,511 - Ac3H11_4987 0.48 +0.0 151,516 + Ac3H11_4987 0.49 -0.4 151,516 + Ac3H11_4987 0.49 +0.3 151,516 + Ac3H11_4987 0.49 +0.9 151,516 + Ac3H11_4987 0.49 -0.5 151,516 + Ac3H11_4987 0.49 -1.0 151,516 + Ac3H11_4987 0.49 +0.5 151,516 + Ac3H11_4987 0.49 -0.0 151,517 - Ac3H11_4987 0.49 -0.1 151,559 - Ac3H11_4987 0.54 -0.2 151,676 + Ac3H11_4987 0.68 -0.3 151,676 + Ac3H11_4987 0.68 +0.6 151,676 + Ac3H11_4987 0.68 -1.2 151,676 + Ac3H11_4987 0.68 -0.1 151,676 + Ac3H11_4987 0.68 -0.4 151,676 + Ac3H11_4987 0.68 +0.3 151,676 + Ac3H11_4987 0.68 +0.6 151,677 - Ac3H11_4987 0.68 -0.5 151,677 - Ac3H11_4987 0.68 +0.4 151,677 - Ac3H11_4987 0.68 -0.0 151,677 - Ac3H11_4987 0.68 -1.2 151,677 - Ac3H11_4987 0.68 +0.4 151,677 - Ac3H11_4987 0.68 -1.5 151,677 - Ac3H11_4987 0.68 +0.3 151,677 - Ac3H11_4987 0.68 -0.1 151,677 - Ac3H11_4987 0.68 -1.9 151,677 - Ac3H11_4987 0.68 +0.2 151,677 - Ac3H11_4987 0.68 +0.2 151,677 - Ac3H11_4987 0.68 -0.1 151,774 + Ac3H11_4987 0.80 +0.7 151,775 - Ac3H11_4987 0.80 +0.5 151,852 + Ac3H11_4987 0.90 +0.8 151,906 + -0.6 151,906 + +0.8 151,907 - -0.0 151,907 - -1.2 151,907 - -0.3 151,907 - +0.3 151,907 - -1.2 152,055 + +1.0 152,206 - Ac3H11_4988 0.15 +0.1 152,206 - Ac3H11_4988 0.15 -0.4 152,235 + Ac3H11_4988 0.17 +0.4 152,235 + Ac3H11_4988 0.17 -0.9 152,235 + Ac3H11_4988 0.17 -0.0 152,235 + Ac3H11_4988 0.17 -0.0 152,235 + Ac3H11_4988 0.17 -0.1 152,236 - Ac3H11_4988 0.17 -0.2 152,236 - Ac3H11_4988 0.17 -0.2 152,236 - Ac3H11_4988 0.17 +0.1 152,502 + Ac3H11_4988 0.32 -0.9 152,502 + Ac3H11_4988 0.32 +0.1 152,502 + Ac3H11_4988 0.32 -0.8 152,503 - Ac3H11_4988 0.32 +0.4 152,503 - Ac3H11_4988 0.32 -0.1 152,503 - Ac3H11_4988 0.32 -0.7 152,623 - Ac3H11_4988 0.38 -0.0 152,623 - Ac3H11_4988 0.38 +0.5 152,623 - Ac3H11_4988 0.38 +0.3 152,854 - Ac3H11_4988 0.51 +0.6 152,919 + Ac3H11_4988 0.55 -0.0 152,919 + Ac3H11_4988 0.55 +0.3 152,920 - Ac3H11_4988 0.55 -0.1 152,931 + Ac3H11_4988 0.55 +0.2 152,931 + Ac3H11_4988 0.55 +0.4 152,931 + Ac3H11_4988 0.55 -0.6 152,932 - Ac3H11_4988 0.55 -0.1 152,932 - Ac3H11_4988 0.55 +0.2 153,066 + Ac3H11_4988 0.63 -1.4 153,066 + Ac3H11_4988 0.63 +0.4 153,066 + Ac3H11_4988 0.63 +0.2 153,066 + Ac3H11_4988 0.63 -2.5 153,066 + Ac3H11_4988 0.63 -0.3 153,067 - Ac3H11_4988 0.63 -0.6 153,067 - Ac3H11_4988 0.63 -0.3 153,067 - Ac3H11_4988 0.63 -0.1 153,067 - Ac3H11_4988 0.63 -1.5 153,067 - Ac3H11_4988 0.63 -0.7 153,067 - Ac3H11_4988 0.63 +0.3 153,219 + Ac3H11_4988 0.71 +0.7 153,220 - Ac3H11_4988 0.71 -0.3 153,220 - Ac3H11_4988 0.71 -0.7 153,220 - Ac3H11_4988 0.71 +0.0 153,220 - Ac3H11_4988 0.71 +0.6 153,516 + Ac3H11_4988 0.88 -0.9 153,693 + -0.5 153,693 + +0.1 153,693 + +0.8 153,694 - -0.1 153,694 - +0.7 153,702 + -0.6 153,702 + -0.2 153,702 + -0.4 153,703 - +0.0 153,703 - -0.0 153,726 + +0.5 153,726 + +0.9 153,727 - -2.2 153,796 + -1.6 153,797 - -0.2 154,214 + Ac3H11_4989 0.47 -0.5 154,214 + Ac3H11_4989 0.47 -0.8 154,214 + Ac3H11_4989 0.47 +0.5 154,215 - Ac3H11_4989 0.47 -0.2 154,253 + Ac3H11_4989 0.52 -0.1 154,253 + Ac3H11_4989 0.52 -0.8 154,254 - Ac3H11_4989 0.52 +0.0 154,254 - Ac3H11_4989 0.52 +0.5 154,472 + Ac3H11_4989 0.79 -1.2 154,726 - -0.3
Or see this region's nucleotide sequence